Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,412,0490TG26.5% 842.4 / 36.8 352intergenic (+25/‑122)proM/aslBtRNA‑Pro/predicted regulator of arylsulfatase activity
Reads supporting (aligned to +/- strand):  ref base T (97/161);  new base G (93/0);  total (191/161)
Fisher's exact test for biased strand distribution p-value = 1.38e-31
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 381 total aligned reads displayed.

GCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTT  >  AP012306/3411900‑3412198
                                                                                                                                                     |                                                                                                                                                      
     acGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGtttttt                                                                                                                                                   <  2:3067663/150‑1 (MQ=255)
       gACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGGTTTTTGt                                                                                                                                                 >  2:1041203/1‑150 (MQ=255)
        aCTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGtt                                                                                                                                                <  2:3553111/150‑1 (MQ=255)
         cTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGGTTTTTGttt                                                                                                                                               >  2:874369/1‑150 (MQ=255)
          tttAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTTGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGGTTTTTGtttt                                                                                                                                              >  1:4214187/1‑150 (MQ=255)
          tttAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCCCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGtttt                                                                                                                                              <  1:538708/150‑1 (MQ=255)
           ttAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTc                                                                                                                                             <  2:3664684/150‑1 (MQ=255)
           ttAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTc                                                                                                                                             <  1:1823335/150‑1 (MQ=255)
           ttAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGGTTTTTGTTTTc                                                                                                                                             >  2:3991876/1‑150 (MQ=255)
           ttAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGGTTTTTGTTTTc                                                                                                                                             >  1:4078839/1‑150 (MQ=255)
             aaaGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCtg                                                                                                                                           <  1:2793919/150‑1 (MQ=255)
             aaaGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCtg                                                                                                                                           >  2:493071/1‑150 (MQ=255)
             aaaGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCtg                                                                                                                                           >  1:3575585/1‑150 (MQ=255)
                    tGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCAttt                                                                                                                                    >  2:1930077/1‑150 (MQ=255)
                     gAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCGCTCGCCGACCAATTTTGAACCCCGCTTCGGTGGGGGTTTTTATTTTCTTTGCCTTTc                                                                                                                                   >  2:3498316/1‑150 (MQ=39)
                      aaCTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCg                                                                                                                                  <  1:3943697/150‑1 (MQ=255)
                       aCTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGt                                                                                                                                 <  1:1098709/150‑1 (MQ=255)
                        cTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTc                                                                                                                                >  2:590818/1‑150 (MQ=255)
                        cTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGGTTTTTGTTTTCTGTGCCTTTCGTc                                                                                                                                >  1:635623/1‑150 (MQ=255)
                         tAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCa                                                                                                                               <  1:756920/150‑1 (MQ=255)
                         tAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCa                                                                                                                               <  1:164113/150‑1 (MQ=255)
                         tAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCa                                                                                                                               <  1:1324595/150‑1 (MQ=255)
                            aaaTTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGGTTTTTGGTTTCTGTGCATTTTGTCAccc                                                                                                                            >  2:1211999/1‑150 (MQ=255)
                             aaTTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGCAGCGCAACTGGTTTGGGACCAGTGGATCGGAGGTTCGAATCCTCGCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTTTTTTCTTTGGATTTTCTCAcact                                                                                                                           >  2:751107/1‑150 (MQ=255)
                              aTTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCAccctc                                                                                                                          >  1:2605913/1‑150 (MQ=255)
                              aTTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGGTTTTTGTTTTCTGTGCATTTCGTCAccctc                                                                                                                          >  2:1404272/1‑150 (MQ=255)
                               ttCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTTTGTGCATTTCGTCAccctcc                                                                                                                         >  2:3638631/1‑150 (MQ=255)
                                    aaaGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCTGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCg                                                                                                                    >  1:2925723/1‑150 (MQ=255)
                                    aaaGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCg                                                                                                                    <  1:2121416/150‑1 (MQ=255)
                                      aGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCa                                                                                                                  <  2:1469329/150‑1 (MQ=255)
                                        cAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGGTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCCTCGCCAt                                                                                                                >  1:3155834/1‑150 (MQ=255)
                                         aGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGGTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATa                                                                                                               >  2:2803678/1‑150 (MQ=255)
                                           tATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGGTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATaaa                                                                                                             >  2:3913526/1‑150 (MQ=255)
                                               tCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGcc                                                                                                         <  2:1686692/150‑1 (MQ=255)
                                                cGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGccc                                                                                                        >  2:369099/1‑150 (MQ=255)
                                                 ggCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGGTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCg                                                                                                       >  2:2741803/1‑150 (MQ=255)
                                                 ggCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGGTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCg                                                                                                       >  2:2992071/1‑150 (MQ=255)
                                                  gCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGt                                                                                                      <  1:2363387/150‑1 (MQ=255)
                                                   cGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGGTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGta                                                                                                     >  2:2232504/1‑150 (MQ=255)
                                                         gcgcAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACt                                                                                               <  1:1200600/150‑1 (MQ=255)
                                                         gcgcAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACt                                                                                               <  2:4268880/150‑1 (MQ=255)
                                                         gcgcAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACt                                                                                               <  2:329180/150‑1 (MQ=255)
                                                            cAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑t                                                                                           <  1:1736188/150‑1 (MQ=255)
                                                              gCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTg                                                                                         <  1:306799/150‑1 (MQ=255)
                                                               cTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGc                                                                                        <  2:3336106/150‑1 (MQ=255)
                                                               cTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGc                                                                                        <  1:3090832/150‑1 (MQ=255)
                                                                 tGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGccc                                                                                      >  1:3678454/1‑150 (MQ=255)
                                                                    tAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCAc                                                                                   >  1:3535979/1‑150 (MQ=255)
                                                                     aGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCCTTTCGTCACCCTCCCCTCGCCATAAACGCCCGTAATAACTCA‑TTGGCCCAca                                                                                  >  1:2129698/1‑149 (MQ=255)
                                                                     aGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACg                                                                                  >  1:2462141/1‑150 (MQ=255)
                                                                         cAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTAt                                                                              <  1:1391353/150‑1 (MQ=255)
                                                                           aCTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGa                                                                            <  2:3364070/150‑1 (MQ=255)
                                                                              ggTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGAttt                                                                         >  1:1545771/1‑150 (MQ=255)
                                                                              ggTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGGTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGAttt                                                                         >  2:4154005/1‑150 (MQ=255)
                                                                               gTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTc                                                                        <  1:348076/150‑1 (MQ=255)
                                                                                tttGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCg                                                                       <  1:1019834/150‑1 (MQ=255)
                                                                                 ttGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGc                                                                      <  2:2466015/150‑1 (MQ=255)
                                                                                 ttGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGGTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCCCGGTATGATTTCGc                                                                      >  2:3247718/1‑150 (MQ=255)
                                                                                   gggACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTTA‑TTGCCCCACGGTATGATTTCGccc                                                                    <  1:4166933/150‑1 (MQ=255)
                                                                                    ggACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGGTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTTCCCCACGGGATGATTTCGCCCt                                                                   >  2:930418/1‑150 (MQ=255)
                                                                                     gACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCtt                                                                  >  1:1469611/1‑150 (MQ=255)
                                                                                     gACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCCTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTTGCCCtt                                                                  >  2:4219317/1‑150 (MQ=255)
                                                                                      aCCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGTCCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTTTTTTATGTGCATTTTGTTACCCTCCCCTCGCAATAAACCCCCCTAATAACTCC‑ATTCCCCACCGTATGAATTCGCCCTTa                                                                 >  2:2000951/1‑150 (MQ=255)
                                                                                      aCCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTa                                                                 <  2:1794015/150‑1 (MQ=255)
                                                                                      aCCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGGTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTa                                                                 >  1:1889146/1‑150 (MQ=255)
                                                                                       ccAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTaa                                                                >  1:3389289/1‑150 (MQ=255)
                                                                                        cAGTGGGTCGGGGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGGTTTTTTTTTTTTGTGCATTTTGTCACCCCCCCCTCGCAATAAAAGCCCGTAATAACTCACTTCCCCCCCGGATGGTTTTCCCCCTTaa                                                                >  2:3168258/1‑150 (MQ=255)
                                                                                         aGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACg                                                              <  1:2023093/150‑1 (MQ=255)
                                                                                           tGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTa                                                            >  1:229941/1‑150 (MQ=255)
                                                                                           tGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGGTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTa                                                            >  2:4108235/1‑150 (MQ=255)
                                                                                                 ggAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAg                                                      <  2:2960924/150‑1 (MQ=255)
                                                                                                 ggAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGGTTTTTGTTTTCTGTGCTTTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTTCCCCACGGTATGATTTCGCCCTTAACGTATTGAAg                                                      >  2:2091549/1‑150 (MQ=255)
                                                                                                   aGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGa                                                    >  1:2533755/1‑150 (MQ=255)
                                                                                                   aGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGGTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGa                                                    >  2:2596004/1‑150 (MQ=255)
                                                                                                    ggTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGAt                                                   <  1:2689491/150‑1 (MQ=255)
                                                                                                      ttCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGGTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGa                                                 >  2:1839327/1‑150 (MQ=255)
                                                                                                       tCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGAc                                                >  2:3781484/1‑150 (MQ=255)
                                                                                                        cGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACt                                               >  1:819498/1‑150 (MQ=255)
                                                                                                          aaTCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTTAAGGATGACTTc                                             >  2:111777/1‑150 (MQ=255)
                                                                                                            tCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAg                                           <  2:2670837/150‑1 (MQ=255)
                                                                                                            tCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGGTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAg                                           >  1:3006026/1‑150 (MQ=255)
                                                                                                             ccTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAgg                                          >  1:445342/1‑150 (MQ=255)
                                                                                                                   tcGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGa                                    <  2:942897/150‑1 (MQ=255)
                                                                                                                   tcGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCCCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATTATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGa                                    >  2:845457/1‑150 (MQ=255)
                                                                                                                       cGACCAATTTTGAACCCCGCTTCGGCGGGGGTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTTAAGGATGACTTCAGGCAAGGAGCGa                                >  2:1540481/1‑150 (MQ=255)
                                                                                                                        gACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGAc                               <  2:2372361/150‑1 (MQ=255)
                                                                                                                         aCCAATTTTGAACCCCGCTTCGGCGGGGGTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGGATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGAcc                              >  2:1761162/1‑150 (MQ=255)
                                                                                                                           cAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCAt                            >  1:159038/1‑150 (MQ=255)
                                                                                                                              ttttGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCAtgct                         >  1:1770074/1‑150 (MQ=255)
                                                                                                                                  gAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCaa                     >  2:1300335/1‑150 (MQ=255)
                                                                                                                                     ccccGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCCCGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAg                  >  2:2895138/1‑150 (MQ=255)
                                                                                                                                        cGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGtt               <  2:2047917/150‑1 (MQ=255)
                                                                                                                                        cGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCCCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGtt               >  2:2540623/1‑150 (MQ=255)
                                                                                                                                         gCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTc              <  2:523635/150‑1 (MQ=255)
                                                                                                                                             cggcggGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAAc          <  1:4001494/150‑1 (MQ=255)
                                                                                                                                             cggcggGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAAc          <  1:1694179/150‑1 (MQ=255)
                                                                                                                                               gcggGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTGGAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAAcgc        <  1:3819967/150‑1 (MQ=255)
                                                                                                                                                cggGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAAcgcg       >  1:923407/1‑150 (MQ=255)
                                                                                                                                                 ggggTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGt      >  1:333417/1‑150 (MQ=255)
                                                                                                                                                 ggggTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGt      >  2:3016314/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCA‑TTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTT  >  AP012306/3411900‑3412198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑