Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,527,3680AC26.2% 1205.0 / 97.9 469L140F (TTA→TTC) rhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
Reads supporting (aligned to +/- strand):  ref base A (287/59);  new base C (0/123);  total (287/182)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 554 total aligned reads displayed.

CGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTG  >  AP012306/3527219‑3527517
                                                                                                                                                      |                                                                                                                                                     
cGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑a                                                                                                                                                       >  2:1828192/1‑150 (MQ=255)
  aTTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑Acc                                                                                                                                                     >  2:1866342/1‑150 (MQ=255)
    ttCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCt                                                                                                                                                   <  1:1165500/150‑1 (MQ=255)
    ttCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCt                                                                                                                                                   >  1:3847036/1‑150 (MQ=255)
     tCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTc                                                                                                                                                  <  1:414655/150‑1 (MQ=255)
      cAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCg                                                                                                                                                 <  1:3243831/150‑1 (MQ=255)
        gTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGAc                                                                                                                                               >  2:1714978/1‑150 (MQ=255)
          tcctcGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCg                                                                                                                                             >  2:3351253/3‑150 (MQ=255)
            ctcGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGcc                                                                                                                                           <  2:4120092/150‑1 (MQ=255)
              cGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAg                                                                                                                                         <  1:615355/150‑1 (MQ=255)
                     cTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCgag                                                                                                                                  >  2:3233000/1‑150 (MQ=255)
                        aaTCTGTGGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCGGGTGCGACGGCGGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑CCCCTCGACCGCCAGTCGCGAGATc                                                                                                                               <  2:3988042/150‑1 (MQ=255)
                          tCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGAAGGAAGGGGAAAATGATTT‑CCCCCCGACCGCCAGTCGCGAGATCtt                                                                                                                             <  2:2722306/150‑1 (MQ=255)
                           cAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTg                                                                                                                            >  1:2710063/1‑150 (MQ=255)
                               tgctgcCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTa                                                                                                                        >  1:3422655/1‑150 (MQ=255)
                               tgctgcCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTa                                                                                                                        >  1:1203382/1‑150 (MQ=255)
                                  tgcCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGc                                                                                                                     >  2:1110699/1‑150 (MQ=255)
                                     cacaAGAGTGGGATGGGCGTTATCCGCTTCCAGGGCGGGTTAACCACGGGTTGTCGGAGCGGGTGCAACAGTTGGTTGCAAAGTTGGAACGGGGGGAAGGGAAAAATAATTT‑ACCCTCGCCCGCCAGTCGCGAGATCTTGTTTATGCAAt                                                                                                                  <  2:2476730/150‑1 (MQ=255)
                                      acaAGAGCTGGATGGGCAGTATCCGTCTCAATGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAAtt                                                                                                                 >  2:761890/1‑150 (MQ=255)
                                              tGGATGGGCAGTATCCGTCTCCCTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑CCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGctct                                                                                                         <  1:1425684/150‑1 (MQ=255)
                                                  tGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtgct                                                                                                     >  1:2470821/1‑150 (MQ=255)
                                                  ggggCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCGGGGGCGACGGCGGGTTGCACAGATGGAACAGCAGGAGGGGAAAAATGTTTT‑CCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtgct                                                                                                     <  2:2439899/149‑1 (MQ=255)
                                                   gggCATTATCCGTCCCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGGGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGTTTT‑CCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtgctg                                                                                                    <  2:1264141/150‑1 (MQ=255)
                                                    ggCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtgctgc                                                                                                   >  1:721440/1‑150 (MQ=255)
                                                       aGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTa                                                                                                >  1:1521056/1‑150 (MQ=255)
                                                        gTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTaa                                                                                               >  1:953880/1‑150 (MQ=255)
                                                        gTATCCGCCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGAACTTGTTTATGCAATTACTGCTCTTGCTGCGTaa                                                                                               >  2:1031253/1‑150 (MQ=255)
                                                          aTCCGTCTCACGGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAAAAGCAGGAAGGGGAAAATGTTTT‑CCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTaaaa                                                                                             <  2:254556/150‑1 (MQ=255)
                                                           tCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCCCAGATGGAACAGCAGGAAGGGGAAAATGATTT‑CCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAg                                                                                            <  1:1300890/150‑1 (MQ=255)
                                                           tCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAg                                                                                            >  1:1687911/1‑150 (MQ=255)
                                                            ccGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGc                                                                                           <  1:1091675/150‑1 (MQ=255)
                                                             cGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCa                                                                                          >  2:2283775/1‑150 (MQ=255)
                                                                 tcACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGttt                                                                                      <  1:3318890/150‑1 (MQ=255)
                                                                  cccTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCCACAGCTGGTTGCACAGAGGGAACAGCAGGAAGGGGAAAATCATTT‑CCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTg                                                                                     <  2:1346470/148‑1 (MQ=255)
                                                                   aCTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGc                                                                                    >  1:1017838/1‑150 (MQ=255)
                                                                     tGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAg                                                                                  >  1:3363905/1‑150 (MQ=255)
                                                                     tGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAg                                                                                  <  1:451284/150‑1 (MQ=255)
                                                                     tGGCGCGTAAACCACAGCGTATTGCAGCAGGGGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAg                                                                                  <  2:3718682/150‑1 (MQ=255)
                                                                       gcgcGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGga                                                                                >  1:4072044/1‑150 (MQ=255)
                                                                        ggcgTTAACCCCGGCTTATTGCGGCAGGTGCGACAGTTGGTTCCCAGGAGGAAACAGCAGGAAGGGGAAAATTATTC‑CCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGgag                                                                               <  2:1967902/149‑1 (MQ=255)
                                                                        cgcgTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGgag                                                                               >  2:3105364/1‑150 (MQ=255)
                                                                          cgTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGaa                                                                             >  1:1364058/1‑150 (MQ=255)
                                                                          cgTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGaa                                                                             >  2:3684071/1‑150 (MQ=255)
                                                                          cgTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGaa                                                                             >  2:1767344/1‑150 (MQ=255)
                                                                          cgTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGaa                                                                             >  1:3634389/1‑150 (MQ=255)
                                                                           gTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAAc                                                                            >  2:710326/1‑150 (MQ=255)
                                                                             tAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGTTTT‑CCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCt                                                                          <  1:2294981/150‑1 (MQ=255)
                                                                              aaCCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTg                                                                         >  2:3561623/1‑150 (MQ=255)
                                                                                 cacaGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGaa                                                                      >  1:2955032/1‑150 (MQ=255)
                                                                                 cacaGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGaa                                                                      <  1:2893367/150‑1 (MQ=255)
                                                                                       gTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAgc                                                                <  1:207331/150‑1 (MQ=255)
                                                                                       gTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAgc                                                                >  1:3739875/1‑150 (MQ=255)
                                                                                       gTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAgc                                                                >  1:3546335/1‑150 (MQ=255)
                                                                                       gTATGGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGAAAAATGATTT‑CCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAgc                                                                <  1:2795802/150‑1 (MQ=255)
                                                                                        tATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAgcg                                                               >  2:3745984/1‑150 (MQ=255)
                                                                                           tGCAGGAGGTGCGACAGCTGGTGGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑CCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCAt                                                            <  1:809154/150‑1 (MQ=255)
                                                                                             cagcagGTGCGACGGTTGGTGGCACAGATGGAACAGCAGAAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACCGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCa                                                          <  2:3510703/150‑1 (MQ=255)
                                                                                             cagcagGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCa                                                          <  1:210460/150‑1 (MQ=255)
                                                                                              agcagGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAc                                                         >  1:2403595/1‑150 (MQ=255)
                                                                                                 agGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGtc                                                      >  1:3586506/1‑150 (MQ=255)
                                                                                                  ggggCGACAGCTGGTTGCACAGAAGAAACAGCAGGAAGGGAAAAATGATTT‑TCCCCCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGtct                                                     <  2:2988813/147‑1 (MQ=255)
                                                                                                  ggTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGtct                                                     >  2:266189/1‑150 (MQ=255)
                                                                                                   gTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGtctc                                                    >  2:1618266/1‑150 (MQ=255)
                                                                                                   gTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGtctc                                                    >  2:593547/1‑150 (MQ=255)
                                                                                                       gACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACt                                                >  1:1846312/1‑150 (MQ=255)
                                                                                                        aCAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑CCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACtt                                               <  1:3969051/150‑1 (MQ=255)
                                                                                                           gCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑CCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCt                                            <  1:1748376/150‑1 (MQ=255)
                                                                                                            cTGGTTGCACGGATGGAACAGCAGGAGGGGAAAAATGTTTT‑CCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCtt                                           <  2:4034470/150‑1 (MQ=255)
                                                                                                            cTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCtt                                           >  1:3931497/1‑150 (MQ=255)
                                                                                                                ttGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctgg                                       >  1:2570059/1‑150 (MQ=255)
                                                                                                                ttGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctgg                                       >  1:2608190/1‑150 (MQ=255)
                                                                                                                 tGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑CCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggc                                      <  1:3470715/150‑1 (MQ=255)
                                                                                                                 tGCACAGATGGAACAGCAGGAAGGGAAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggc                                      <  2:1378873/150‑1 (MQ=255)
                                                                                                                  gCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcc                                     >  2:1685155/1‑150 (MQ=255)
                                                                                                                   cacaGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcct                                    >  1:3032299/1‑150 (MQ=255)
                                                                                                                     caGATGCAAGAGCAAGAAGGGGAAAGTCATTT‑CCCCTCGCCCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcctgg                                  <  2:2848856/150‑1 (MQ=255)
                                                                                                                        agggAACGGCAGGAAGGGGAAAATGATTT‑CCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCctggctg                               <  2:2164859/148‑1 (MQ=255)
                                                                                                                         tGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCctggctgg                              >  2:629217/1‑150 (MQ=255)
                                                                                                                         tGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCctggctgg                              >  1:877917/1‑150 (MQ=255)
                                                                                                                          ggAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGTCCTGGCTgga                             >  1:4209630/1‑150 (MQ=255)
                                                                                                                            aaCAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTggagg                           >  2:918983/1‑150 (MQ=255)
                                                                                                                              cagcagGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGAc                         >  2:3051437/1‑150 (MQ=255)
                                                                                                                                gcagGAAGGGGAAAATGATTT‑CCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCa                       <  1:2852600/150‑1 (MQ=255)
                                                                                                                                gcagAAGGGGGAAAATAATTC‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCa                       <  2:425253/150‑1 (MQ=255)
                                                                                                                                 cagGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAt                      >  1:921015/1‑150 (MQ=255)
                                                                                                                                  agGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAtt                     >  2:3787517/1‑150 (MQ=255)
                                                                                                                                      aGGGGAAATTTATTTCCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTg                  <  2:2072534/150‑1 (MQ=255)
                                                                                                                                      aGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGc                 >  1:3152864/1‑150 (MQ=255)
                                                                                                                                       ggggAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGcc                >  2:3864003/1‑150 (MQ=255)
                                                                                                                                         ggAAAATGATTT‑CCCCCCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGa              <  2:1178272/150‑1 (MQ=255)
                                                                                                                                          gAAAATTTTTT‑CCCCCAGCCCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGAt             <  2:1509231/150‑1 (MQ=255)
                                                                                                                                           aaaaTGATTT‑CCCTTCATCCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATg            <  2:504801/150‑1 (MQ=255)
                                                                                                                                           aaaaTGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATg            >  1:1982886/1‑150 (MQ=255)
                                                                                                                                             aaTGATTT‑CCCCTCGCCCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAg          <  2:3929978/150‑1 (MQ=255)
                                                                                                                                               tatttt‑CCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCCGGCCTGGCTGGAGGACCATTTTGCCGATGAGGt        <  2:2910792/147‑1 (MQ=255)
                                                                                                                                               tGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGt        >  2:1944540/1‑150 (MQ=255)
                                                                                                                                                   tt‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAAt    >  1:2205993/1‑150 (MQ=255)
                                                                                                                                                    t‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAAtt   >  1:3089424/1‑150 (MQ=255)
                                                                                                                                                      aCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTg  <  1:549319/150‑1 (MQ=255)
                                                                                                                                                      aCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTg  >  1:1609839/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTT‑ACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTG  >  AP012306/3527219‑3527517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑