Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,938,8870CT31.2% 361.2 / 131.2 199S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (12/125);  new base T (62/0);  total (74/125)
Fisher's exact test for biased strand distribution p-value = 8.78e-40
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 240 total aligned reads displayed.

ACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACG  >  AP012306/3938738‑3939036
                                                                                                                                                     |                                                                                                                                                      
aCTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttc                                                                                                                                                        <  1:3588002/150‑1 (MQ=255)
   cATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttcttt                                                                                                                                                     <  1:3300468/150‑1 (MQ=255)
   cATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGACTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttcttt                                                                                                                                                     <  2:1408591/150‑1 (MQ=255)
       gAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTttttctttttct                                                                                                                                                 <  2:3398944/150‑1 (MQ=255)
        aaaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTg                                                                                                                                                <  2:2314194/150‑1 (MQ=255)
         aaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGCCAtttttttttttttttt                                                                                                                                               >  2:400284/1‑146 (MQ=255)
            aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCAt                                                                                                                                            >  2:1908900/1‑150 (MQ=255)
            aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCAt                                                                                                                                            >  1:2034626/1‑150 (MQ=255)
               cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATg‑ag                                                                                                                                        <  1:1102512/150‑1 (MQ=255)
                gTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATg‑aga                                                                                                                                       <  1:2479617/150‑1 (MQ=255)
                 tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGaa                                                                                                                                      <  1:965373/150‑1 (MQ=255)
                 tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGaa                                                                                                                                      <  2:2736999/150‑1 (MQ=255)
                   aTGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATG‑AGAAgg                                                                                                                                    >  1:1564614/1‑149 (MQ=255)
                    tgtgtagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGAACTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTg                                                                                                                                   <  2:1571304/147‑1 (MQ=255)
                    tGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTG‑AGAAGTg                                                                                                                                   >  1:2939255/1‑150 (MQ=255)
                     gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGa                                                                                                                                  <  1:1018270/150‑1 (MQ=255)
                        tagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAgcg                                                                                                                               <  2:580026/150‑1 (MQ=255)
                         agtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAgcgc                                                                                                                              <  1:811799/150‑1 (MQ=255)
                          gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATT‑AGAAGTGAGGGCa                                                                                                                             >  1:2882708/1‑150 (MQ=255)
                              cGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCagaga                                                                                                                         <  1:1394815/150‑1 (MQ=255)
                                gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATT‑AGAAGGGAGCGCAGAGAgg                                                                                                                       >  1:859797/1‑150 (MQ=255)
                                 tATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAggg                                                                                                                      <  1:434655/150‑1 (MQ=255)
                                   ttGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTTCCTG‑AGAAGTGAGCGGAGAGAGGGAt                                                                                                                    >  1:2868282/1‑150 (MQ=255)
                                    tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGAtt                                                                                                                   <  1:1352655/150‑1 (MQ=255)
                                    tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGAGTTTTTTTTTTTTTTGCCCTT‑AGAGGAAGGAGCAGAGGGGGaaa                                                                                                                   >  2:2101802/1‑148 (MQ=255)
                                      agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTAt                                                                                                                 <  2:723924/150‑1 (MQ=255)
                                         gTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCCTT‑GGAAGTGAGCGGCGGGAGGGGTTTTCtt                                                                                                              >  2:1528382/1‑150 (MQ=255)
                                          tttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTT‑AGAAGTGAGGGCCGAGAGGGGTTATCttt                                                                                                             >  1:670662/1‑149 (MQ=255)
                                           ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATT‑AGAAGTGAGGGCAGAGAGGGAGTATCTTGc                                                                                                            >  2:1265300/1‑150 (MQ=255)
                                           ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATG‑AGAAGTGAGGGGAGGGGGGGGGTATCtttg                                                                                                            >  2:2326122/1‑148 (MQ=255)
                                            tATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTTCCTG‑AGAAGGGAAGGCAGGAAGGGGTTTTCttttc                                                                                                           >  2:1363293/1‑147 (MQ=255)
                                             aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTG‑AGAAGTGAGCGCAGAGAGGGGTTATCTTTCct                                                                                                          >  1:2386865/1‑150 (MQ=255)
                                               tCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCctga                                                                                                        <  2:3564295/150‑1 (MQ=255)
                                                cAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCctgac                                                                                                       <  1:1159314/150‑1 (MQ=255)
                                                 aGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATT‑AGAAGTGAGGGGAGAGGGGGAGTATCTTTCctgact                                                                                                      >  2:1239535/1‑150 (MQ=255)
                                                  gAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCctgactg                                                                                                     <  2:4147702/150‑1 (MQ=255)
                                                     aGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTGACTGAAc                                                                                                  >  2:2652912/1‑150 (MQ=255)
                                                       tttGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTg                                                                                                <  1:3963018/150‑1 (MQ=255)
                                                         tGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGc                                                                                              <  1:753393/150‑1 (MQ=255)
                                                           cGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATG‑AGGAGTGAGCGGAGAGAGGGGTTATCTTTCCTGTCTGGACttggga                                                                                            >  2:266983/1‑145 (MQ=255)
                                                           cGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTG‑AGAAGTGGGCGCAGAGAGGGGGTATTTTTCCTGACTGAACTGGCgg                                                                                            >  1:1062594/1‑149 (MQ=255)
                                                            gggTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCATGAAGAAG‑GAGCGCGGAGGGGGATTTTCTTTCCTGGCTGAAATGGCGaa                                                                                           >  2:1952862/1‑149 (MQ=255)
                                                            gggTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTG‑AGAAGTGAGCGCAGAGAGGGGTTTTTTTGCCTGACTGGACTGGCggt                                                                                           >  2:2434230/1‑148 (MQ=255)
                                                            gggTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGAt                                                                                           <  1:4083066/150‑1 (MQ=255)
                                                               tAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTaa                                                                                        <  2:1496428/150‑1 (MQ=255)
                                                                 aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATG‑AGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGGTTaaat                                                                                      >  2:2286500/1‑148 (MQ=255)
                                                                   ccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATG‑AGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTTAACTGGCGGTTAAAttt                                                                                    >  2:1427821/1‑150 (MQ=255)
                                                                      tataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTTCCTG‑AGAAAGGGGGGCAGAGAGGGGTTATCTTTCCTTGCTTGACTGGCGGTTAAGttttcc                                                                                 >  2:1117576/1‑147 (MQ=255)
                                                                              ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATG‑AGAAGGGAGCGCAGAGAGGGGTTATCTTTCCTGACTGAACTGGCGGTTAAATTTCCCCGCAcccc                                                                         >  2:3595078/1‑149 (MQ=255)
                                                                              ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATG‑AGAAAAGGGGGCCGGGAGGGGGTATTTTTTCTGCCTGGACTGGCGGATAAATTTTcgcgctcccc                                                                         >  2:4224065/1‑144 (MQ=255)
                                                                                aTACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGc                                                                       <  1:4000768/150‑1 (MQ=255)
                                                                                aTACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGc                                                                       <  2:3726086/150‑1 (MQ=255)
                                                                                  aCCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGt                                                                     <  1:2588438/150‑1 (MQ=255)
                                                                                   ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGtt                                                                    <  2:897454/150‑1 (MQ=255)
                                                                                       taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGt                                                                <  2:3709801/150‑1 (MQ=255)
                                                                                        aCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGtt                                                               <  2:2799729/150‑1 (MQ=255)
                                                                                           aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTAtt                                                            <  2:2859188/150‑1 (MQ=255)
                                                                                            gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTg                                                           <  1:556454/150‑1 (MQ=255)
                                                                                            gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTg                                                           <  1:182552/150‑1 (MQ=255)
                                                                                             tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTAATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGc                                                          <  2:246415/150‑1 (MQ=255)
                                                                                              cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCg                                                         <  1:641756/150‑1 (MQ=255)
                                                                                              cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCg                                                         <  2:2994099/150‑1 (MQ=255)
                                                                                                 gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgat                                                      <  2:1820663/150‑1 (MQ=255)
                                                                                                       tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatgat                                                <  2:1056940/150‑1 (MQ=255)
                                                                                                          cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATAtt                                             <  1:516351/150‑1 (MQ=255)
                                                                                                           aGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTTTCATG‑AGAAGTGAGCGCAGAGAGGGATTTTTTTTCCTTACTTAAATTGGGGTTTAGTTTTCCCCTACCCCGCGGTTTATTATTTTGGATGATGataatt                                            >  2:90855/1‑147 (MQ=255)
                                                                                                             gATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGaa                                          <  2:4154400/150‑1 (MQ=255)
                                                                                                               tGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTG‑AGAAGTGAGCGCAGAGAGGGGTTATTTTTCCTGACTGAACTGGGGGTTAAGTTTTCGCGGACCCCGCGGTTTGTTTTTTCGGGTGGTTATATTTAAga                                        >  2:1455531/1‑149 (MQ=255)
                                                                                                                 ccATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTc                                      <  2:3130429/150‑1 (MQ=255)
                                                                                                                  cATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTG‑AGAAGTGGGCGCAGAGAGGGGTTATCTTTCCTTACTGAACTTGCGATTTAGTTTTCCCGTACCCGGCGGTTTGTTTTTTCGGGTGATGATATTTaaagtcg                                     >  2:407111/1‑145 (MQ=255)
                                                                                                                  cATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCg                                     <  1:2818271/150‑1 (MQ=255)
                                                                                                                   aTGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCgg                                    <  2:3931968/150‑1 (MQ=255)
                                                                                                                    tGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGc                                   <  2:2582745/150‑1 (MQ=255)
                                                                                                                    tGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGc                                   <  2:2127634/150‑1 (MQ=255)
                                                                                                                     gTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTG‑AGAAGGGGGGGGAGAGAGGGGGTTTTTTTCCTTACTGAACTGGCGATTTAGTTTTCCCGGACCCGGCGGTTTGTTTTTTTGGGTGGTTGTATTTAAACTCgggg                                  >  2:3642113/1‑148 (MQ=255)
                                                                                                                      tCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTg                                 <  1:2246044/150‑1 (MQ=255)
                                                                                                                      tCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTg                                 <  2:4267481/150‑1 (MQ=255)
                                                                                                                         cgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGAtt                              <  2:11505/150‑1 (MQ=255)
                                                                                                                           gATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTgg                            <  1:2078379/150‑1 (MQ=255)
                                                                                                                            aTCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGt                           <  1:3672493/150‑1 (MQ=255)
                                                                                                                             tctcGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTG‑AGAAGGGAGGGCAGAGAGGGGGTTTTTTTCCTTACTGTACTGGCGATTAAGTTTTCGCCGACCCGGCGGTTTGTTTTTTTGGGTGGTTGTATTTAAGCCCGGCTGGTTgggt                          >  2:1196003/1‑148 (MQ=255)
                                                                                                                             tctcGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGtt                          <  1:1802369/150‑1 (MQ=255)
                                                                                                                               tcGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCa                        <  1:414800/150‑1 (MQ=255)
                                                                                                                               tcGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCa                        <  1:2357018/150‑1 (MQ=255)
                                                                                                                                cGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTG‑AGAAATGAGCGCAGAGAGGGGTTATCTTTCCTGACTTAACTGGCGATTTAGTTTTCGCGTACCCGGCGGTTTGTTATTTCGGATGATGATATTTTAGCTCGGCTGGTTGGTTTAt                       >  2:688984/1‑150 (MQ=255)
                                                                                                                                 gTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAtt                      <  2:417026/150‑1 (MQ=255)
                                                                                                                                  tttGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTg                     <  1:3689235/150‑1 (MQ=255)
                                                                                                                                    tGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTc                   <  1:1939777/150‑1 (MQ=255)
                                                                                                                                           gTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCa            >  1:2803380/1‑150 (MQ=255)
                                                                                                                                            tCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAc           <  1:1720779/150‑1 (MQ=255)
                                                                                                                                             cATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAcc          >  1:3536103/1‑150 (MQ=255)
                                                                                                                                              aTTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCg         >  1:2292527/1‑150 (MQ=255)
                                                                                                                                              aTTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCg         <  2:3493828/150‑1 (MQ=255)
                                                                                                                                              aTTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCg         >  2:3190325/1‑150 (MQ=255)
                                                                                                                                              aTTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCg         <  1:3851762/150‑1 (MQ=255)
                                                                                                                                              aTTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCg         >  1:1210063/1‑150 (MQ=255)
                                                                                                                                                 ttttctttttctGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTg      >  1:3030796/1‑150 (MQ=255)
                                                                                                                                                 ttttctttttctGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTg      >  2:3016769/1‑150 (MQ=255)
                                                                                                                                                 ttttctttttctGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTg      <  1:133601/150‑1 (MQ=255)
                                                                                                                                                  tttctttttctGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTgg     >  1:4056919/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
ACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATG‑AGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACG  >  AP012306/3938738‑3939036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑