Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306257,1370TG20.5% 539.7 / 11.2 221L496V (TTG→GTG) betTcholine transporter of high affinity
Reads supporting (aligned to +/- strand):  ref base T (127/47);  new base G (45/0);  total (174/47)
Fisher's exact test for biased strand distribution p-value = 7.23e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 300 total aligned reads displayed.

CCCCCGGCTGGCTGCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTACACTAAACAG  >  AP012306/256988‑257286
                                                                                                                                                     |                                                                                                                                                     
 ccccGGCTGGCTGCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGtt                                                                                                                                                      <  1:2351492/150‑1 (MQ=255)
 ccccGGCTGGCTGCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGtt                                                                                                                                                      <  1:2639748/150‑1 (MQ=255)
    cGGCTGGCTGCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGta                                                                                                                                                   <  1:3007685/150‑1 (MQ=255)
     ggctggctGCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGtat                                                                                                                                                  <  1:824161/150‑1 (MQ=255)
      gctggctgCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGtata                                                                                                                                                 >  1:3835947/1‑150 (MQ=255)
       ctggctgCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTTTGATCTTCTTCGTGATGGCGGGGTTGTATaa                                                                                                                                                <  2:3244945/150‑1 (MQ=255)
       ctggctgCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATaa                                                                                                                                                <  1:4037209/150‑1 (MQ=255)
        tggctgCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATaaa                                                                                                                                               <  1:1090180/150‑1 (MQ=255)
         ggctgCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAAt                                                                                                                                              >  2:3604023/1‑150 (MQ=255)
           ctgCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAAtct                                                                                                                                            <  2:2502103/150‑1 (MQ=255)
           ctgCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAAtct                                                                                                                                            <  1:582957/150‑1 (MQ=255)
                cgTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGaa                                                                                                                                       <  1:1234447/150‑1 (MQ=255)
                 gTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAg                                                                                                                                      >  1:3273097/1‑150 (MQ=255)
                   cttctGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGt                                                                                                                                    <  1:3243608/150‑1 (MQ=255)
                    ttctGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTa                                                                                                                                   >  2:3042016/1‑150 (MQ=255)
                     tctGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTag                                                                                                                                  <  1:2274969/150‑1 (MQ=255)
                     tctGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTag                                                                                                                                  <  1:3084414/150‑1 (MQ=255)
                      ctGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTaga                                                                                                                                 <  1:3883921/150‑1 (MQ=255)
                      ctGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGTaga                                                                                                                                 >  1:3108833/1‑150 (MQ=255)
                       tGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTagaa                                                                                                                                >  1:3439952/1‑150 (MQ=255)
                        ggTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTagaag                                                                                                                               <  1:249376/150‑1 (MQ=255)
                          tCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGAt                                                                                                                             <  1:4797002/150‑1 (MQ=255)
                             gTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGTAGAAGATTAc                                                                                                                          >  2:3299662/1‑150 (MQ=255)
                                  gATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGTAGAAGATTAccgccg                                                                                                                     >  2:2904125/1‑150 (MQ=255)
                                    ttGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTg                                                                                                                   >  2:3576767/1‑150 (MQ=255)
                                        ccTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAg                                                                                                               >  2:4401944/1‑150 (MQ=255)
                                             tgACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGGAGAAGATTACCGCCGTGAAAGTGCCa                                                                                                          >  1:5251346/1‑150 (MQ=255)
                                               aCGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAAc                                                                                                        >  1:430458/1‑150 (MQ=255)
                                                 gCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACcg                                                                                                      >  1:4886934/1‑150 (MQ=255)
                                                 gCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGAGCCAACcg                                                                                                      >  2:3518726/1‑150 (MQ=255)
                                                     ggCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCCCGAt                                                                                                  >  1:4423509/1‑150 (MQ=255)
                                                         tGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGGAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATaacg                                                                                              >  2:2093287/1‑150 (MQ=255)
                                                            tgatgaCTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGGAGAAGATTACCGCCGTGGAAGTGCCAACCGCGATaccgcac                                                                                           >  1:1817980/1‑150 (MQ=255)
                                                               tgaCTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATaccgcaccgc                                                                                        >  1:1401360/1‑150 (MQ=255)
                                                                 aCTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGa                                                                                      >  2:510278/1‑150 (MQ=255)
                                                                  cTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGAc                                                                                     >  1:1747405/1‑150 (MQ=255)
                                                                   tAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGAcc                                                                                    >  2:716604/1‑150 (MQ=255)
                                                                     aCGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGc                                                                                  >  1:3673655/1‑150 (MQ=255)
                                                                        ggATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCCCACCGCGACCGCTgg                                                                               >  2:4388188/1‑150 (MQ=255)
                                                                         gATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTggg                                                                              >  1:4065177/1‑150 (MQ=255)
                                                                           tatCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGGAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGc                                                                            >  1:933422/1‑150 (MQ=255)
                                                                                gcgcTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGCTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATAACGCACCACGACCGGTCGGGCTTCAg                                                                       >  2:107015/1‑150 (MQ=255)
                                                                                  gcTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGa                                                                     >  2:1950728/1‑150 (MQ=255)
                                                                                   cTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGAt                                                                    >  1:2124599/1‑150 (MQ=255)
                                                                                     gCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAAACTCTGGAGGGAGAAGATTACCGCCGGGAAAGAGCCAACCGCGATACCGCACCGCGACCGCTGGGGCCTCAGGAtgg                                                                  >  2:3659752/1‑148 (MQ=255)
                                                                                        aaaCACCGGGGTGTTTGGGGGCCTGCCTTTCGGGTTTGTGATTTTCTGCGTGTGGGGGGGGTTGTAAAAATCTCGGAGGGAAGAAGTTTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCt                                                               <  2:4973288/150‑1 (MQ=255)
                                                                                        aaaCACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCCCGACCGCCGGGGCTTCAGGATCGCCt                                                               >  2:4487466/1‑150 (MQ=255)
                                                                                         aaCACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTg                                                              >  1:2860035/1‑150 (MQ=255)
                                                                                         aaCACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTg                                                              >  2:3749408/1‑150 (MQ=255)
                                                                                          acacCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCCCACCGCGACCGCTGGGGCTTCAGGATCGCCTGa                                                             >  2:1481546/1‑150 (MQ=255)
                                                                                          acacCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGa                                                             >  2:545089/1‑150 (MQ=255)
                                                                                             ccacGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCt                                                          >  2:4517235/1‑150 (MQ=255)
                                                                                               agggTGATTGGGGGGCTGCCTTTCGGTTTTGTATTCTTCTTGGGGGGGGGGGGGTTGTATAAATCTCGGAGGGAAGAAGATTCCCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTgg                                                        <  2:1722056/148‑1 (MQ=255)
                                                                                                cGGGGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGGGATGGCGGGGGGGTATTAATCTCTGAAGGGAAAAGAATACCGTCGTTGGCGGGCCAACAGCGAAACCCCACCGCGACGGCTGGGGGTTCAGGATCGCCTTCGCTGGa                                                       >  2:1282733/1‑150 (MQ=255)
                                                                                                 ggTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGGATAAATCTCTGTAAGAAGAAGATTACCGCCGTGGAAATGCCCAACCCGATACCGCACCCCGACCGCTGTGGCTTCAGGATCGCCTGTGCTGGaa                                                      >  2:5145727/1‑150 (MQ=255)
                                                                                                  gTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGaaa                                                     >  1:2576401/1‑150 (MQ=255)
                                                                                                     aTTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTTGaaaaaa                                                  >  2:5198683/1‑150 (MQ=255)
                                                                                                     aTTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGaaaaaa                                                  >  2:1445152/1‑150 (MQ=255)
                                                                                                      ttATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAAc                                                 >  1:1024109/1‑150 (MQ=255)
                                                                                                       tATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGGAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACg                                                >  2:13667/1‑150 (MQ=255)
                                                                                                        aTGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAAGTAAAAGATTACCCCCGTGAAAGTGCCGACCGCGGTACCCCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGt                                               >  2:4673562/1‑150 (MQ=255)
                                                                                                         gggggCTGCCTTTCAGTTTTGTTTTTTTCTGGGGGGGGGGGGGTTTGAATAACTCTGGGAGGGAAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGtc                                              <  2:5083709/149‑1 (MQ=255)
                                                                                                          ggggCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGtct                                             >  1:3337484/1‑150 (MQ=255)
                                                                                                          ggggCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGGAAAAACGtct                                             >  1:1933941/1‑150 (MQ=255)
                                                                                                           gggCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAACATTACCGCCGTGGAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGGAAAAACGtctc                                            >  2:3982068/1‑150 (MQ=255)
                                                                                                            ggCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGGAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGtctct                                           >  1:2904590/1‑150 (MQ=255)
                                                                                                              cTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTcg                                         >  1:518798/1‑150 (MQ=255)
                                                                                                              cTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGCAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCCCCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACCGCTCTcg                                         >  2:806525/1‑150 (MQ=255)
                                                                                                               tGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGGAGAAGATTACCGCCGTGAAAGGGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGGCTCTcgc                                        >  2:921604/1‑150 (MQ=255)
                                                                                                               tGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTcgc                                        >  1:645982/1‑150 (MQ=255)
                                                                                                                gctgTTCAGTTTTGTGTTTTTCTTGGTGTGGGGGGGTTTATATAAATCTGAGAGGGTAGAAGTTTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTcgcg                                       <  2:184192/147‑1 (MQ=255)
                                                                                                                  cGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGcc                                     >  1:2757588/1‑150 (MQ=255)
                                                                                                                  cGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGAACGCCTGAGCTGGAAAAAACGTCTCTCGCGcc                                     >  1:924069/1‑150 (MQ=255)
                                                                                                                   gTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATTTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCt                                    >  2:48730/1‑150 (MQ=255)
                                                                                                                      catttttGTGATCTTCTTCGTGTGGGGGGGTTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCtgat                                 <  1:4951865/146‑1 (MQ=255)
                                                                                                                       aGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTTTTAATTTCTGAAGGTAGAAGATTACCCCCGTGAAAGAGCCAAACGCGATAACGCCCCGCGAACGCTGGGGGGTCAGGATCGCCTGAGATGGGAAAAACGTCTCTCGCGCCtgatg                                >  2:116269/1‑150 (MQ=255)
                                                                                                                         ttttGTGTTCTTCTTGGTGGGGGGGGGTTTGAATAAATCTCTGAAGGAAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGaa                              <  1:2784331/149‑1 (MQ=255)
                                                                                                                         cTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGaa                              >  2:3174719/1‑150 (MQ=255)
                                                                                                                         cTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCCCGCCTGATGaa                              >  2:2741429/1‑150 (MQ=255)
                                                                                                                          tttGTGATCTTCTTCGTGATGGCGGGGGTGTTTAAATATCTGCAGGTGGCGGATTACCGCCGTGTAAGTTCCAAACGCGATACCGCACCCCGTCCCCTGGGGGCTTGTCATCGCCTTTGATCGCAAAAACCTCTCTCGCGCCTGATGaaa                             >  2:264894/1‑149 (MQ=255)
                                                                                                                           ttGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAAtt                            >  2:42451/1‑150 (MQ=255)
                                                                                                                           ttGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAAtt                            >  2:2419329/1‑150 (MQ=255)
                                                                                                                              tcaTCTTTTTCGTGGGGGGGGGGTGTAATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATc                         <  1:5143963/148‑1 (MQ=255)
                                                                                                                               gATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGGAGAAGATTACCGCCGTGAAAGGGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATcc                        >  2:1612012/1‑150 (MQ=255)
                                                                                                                                      ttcGTGATGGCGGGGGTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCCCACCGCGACCGCTGGGGCTTCAGGAGCGCCTGAGCTGGAAAAAACCTCTCTCGCGCCTGATGAATTATCCGGGCAcg                 >  2:405638/1‑150 (MQ=255)
                                                                                                                                        cGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCAcgcg               >  1:4656661/1‑150 (MQ=255)
                                                                                                                                        cGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCAcgcg               >  1:487288/1‑150 (MQ=255)
                                                                                                                                        cGTGATGGCGGGGGTGTATAAATCTCTGAAGGGAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCAcgcg               >  2:2715778/1‑150 (MQ=255)
                                                                                                                                         gTGATGGCGGGGTTGTATAAATCTCTGAAGGGAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGt              >  1:4244364/1‑150 (MQ=255)
                                                                                                                                          tGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGtt             >  1:5068631/1‑150 (MQ=255)
                                                                                                                                           gggggggggtTTGTAAAAATCTGTGAAGGAAAAAGTTTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTa            <  1:1137317/144‑1 (MQ=255)
                                                                                                                                           gATGGCGGGGGTGTATAAATCTCTGAAGGGAGAAGATTTCCGCCGGGAAAGTGCCAACCCCGAAACCGCCCCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAAAGTCTCTCGCGCCTGATGAAATATCCGGGCACGCGTTa            >  2:2296753/1‑150 (MQ=255)
                                                                                                                                             tGGCGGGGTGATAAAAATCTCGGAGGAAAAATTATTGCCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTaca          <  1:4611770/150‑1 (MQ=255)
                                                                                                                                                gggggTTTTAAAAATCTCTGAAGGTAGAATTTTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTACACTa       <  1:2215483/149‑1 (MQ=255)
                                                                                                                                                cGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATTGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTACACTa       >  2:3452800/1‑150 (MQ=255)
                                                                                                                                                cGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTACACTa       >  1:766284/1‑150 (MQ=255)
                                                                                                                                                cGGGGGTGTATAAATCTCTGAAGGGAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTACACTa       >  2:887679/1‑150 (MQ=255)
                                                                                                                                                   gtttGTATAATTTTCTAAAGGGAGAGGTTTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTACACTAAAc    <  1:454727/148‑1 (MQ=255)
                                                                                                                                                     ttGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGGTCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTACACTAAACAg  >  1:4724165/1‑150 (MQ=255)
                                                                                                                                                     ttGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTACACTAAACAg  >  2:3856195/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCCCCGGCTGGCTGCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTACACTAAACAG  >  AP012306/256988‑257286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑