Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306935,0540GT20.4% 273.6 / 36.3 93intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
Reads supporting (aligned to +/- strand):  ref base G (19/55);  new base T (19/0);  total (38/55)
Fisher's exact test for biased strand distribution p-value = 1.23e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 167 total aligned reads displayed.

AGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGC‑GC‑AGGGGGCGGCCAATCGCCGCCGCCCCCTGC‑TGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACA  >  AP012306/934908‑935203
                                                                                                                                                    |                                                                                                                                                         
  tATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑ttttt‑ttttg                                                                                                                                                     >  1:4610449/1‑148 (MQ=255)
  tATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑gtttg‑ttttg                                                                                                                                                     >  1:3078246/1‑148 (MQ=255)
  tATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTgg                                                                                                                                                     >  1:1517541/1‑150 (MQ=255)
    ttGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑gtttg‑ttttggg                                                                                                                                                   >  2:1708723/1‑146 (MQ=255)
    ttGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTT‑TTTTGCt                                                                                                                                                   >  1:4641107/1‑150 (MQ=255)
     tGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCtt                                                                                                                                                  <  1:1906760/150‑1 (MQ=255)
      gggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑ATTTT‑TTTTGCttt                                                                                                                                                 >  2:3225066/1‑149 (MQ=255)
       ggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGGGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTca                                                                                                                                                <  2:1715211/150‑1 (MQ=255)
       ggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGG‑TTTTT‑TTTGGTTTc                                                                                                                                                 >  2:128120/1‑150 (MQ=255)
        gATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTTGCTTtag                                                                                                                                               >  1:4662230/1‑150 (MQ=255)
          ttGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTT‑TTTTGGTTctgca                                                                                                                                             >  2:5206447/1‑150 (MQ=255)
          ttGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTcagca                                                                                                                                             <  1:5013594/150‑1 (MQ=255)
           tGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTTGCTTcagcag                                                                                                                                            >  1:3480164/1‑150 (MQ=255)
           tGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTcagcag                                                                                                                                            <  2:4226319/150‑1 (MQ=255)
           tGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTcagcag                                                                                                                                            <  1:3716714/150‑1 (MQ=255)
           tGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTT‑TTTTGTTTctggag                                                                                                                                            >  2:2136709/1‑145 (MQ=255)
           tGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTG‑TTTTGCTTctgcag                                                                                                                                            >  1:1429044/1‑150 (MQ=255)
             tAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTT‑TTTGTGTTcaccagac                                                                                                                                          >  2:5101418/1‑148 (MQ=255)
             tAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑CTTTT‑TTTTGCTTcaaccacc                                                                                                                                          >  2:1184690/1‑144 (MQ=255)
                    tAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTTGCTTCAGCAGCGGGGTgcg                                                                                                                                   >  1:409513/1‑150 (MQ=255)
                    tAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTgcg                                                                                                                                   <  1:2039352/150‑1 (MQ=255)
                    tAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTgcg                                                                                                                                   <  1:1147950/150‑1 (MQ=255)
                     aGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTT‑TTTTGCTTCAGCCGCGGGGTgggc                                                                                                                                  >  2:770370/1‑150 (MQ=255)
                        ttGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTTGCTTCCGCACCGGGGTGGGCGcc                                                                                                                               >  1:3631599/1‑150 (MQ=255)
                        ttGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTcc                                                                                                                               <  1:4201309/150‑1 (MQ=255)
                         tGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTTGCGTCAGCAACGGGGTgccgttcc                                                                                                                              >  1:2976600/1‑144 (MQ=255)
                         tGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTT‑TTTTTTCTCTGCCGCGGGGTGCGCcccc                                                                                                                              >  1:3799856/1‑150 (MQ=255)
                         tGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTT‑TTTTGCTTTCGCAGCGGGGTGCGCTccc                                                                                                                              >  2:288548/1‑150 (MQ=255)
                          gCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTT‑TTTGGCTTCAGCAGCGGGTTGCGCTcccc                                                                                                                             >  1:728309/1‑149 (MQ=255)
                          gCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTTGCTTCAGCAGCGGGGTGCGCTcccc                                                                                                                             >  1:390129/1‑149 (MQ=255)
                           cTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCtt                                                                                                                            <  1:3355631/150‑1 (MQ=255)
                           cTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCtt                                                                                                                            <  2:1907903/150‑1 (MQ=255)
                           cTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCtt                                                                                                                            <  1:1823486/150‑1 (MQ=255)
                           cTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAAGGACTTAGCCTGCGGC‑GG‑ATTTT‑TTTTGGTTCACCAACGGGGGGGGCTcccct                                                                                                                            >  2:416341/1‑148 (MQ=255)
                            tGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTGTTTTGGTTTCA‑CCGCGGGGTGCGCTcccctt                                                                                                                           >  1:330/1‑147 (MQ=255)
                              ggTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTT‑TTTTGGCTCCGCAGCGGGGTGGGCcccccttat                                                                                                                         >  1:436915/1‑145 (MQ=255)
                              ggTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTGGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAAt                                                                                                                         <  2:3131998/150‑1 (MQ=255)
                               gTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTTGCTTCAGCAGCGGGGTGCGCTCCCCTTAtg                                                                                                                        >  1:1558304/1‑150 (MQ=255)
                                tAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAAtgt                                                                                                                       <  1:4838172/150‑1 (MQ=255)
                                 aaaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTTGGTTCAGCAGCGGGGTGCGCCCCCCTAAtttt                                                                                                                      >  1:4443591/1‑147 (MQ=255)
                                    gcgcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTT‑TTTTGCTTCAGCAGCGGGTTGCGCTCCCCTTATGTTCCt                                                                                                                   >  2:689730/1‑150 (MQ=255)
                                      gcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTT‑TTTTGGTTCCGCAGCGGGGTGGGCTCCCCTTATTTGGCTcc                                                                                                                 >  2:4248709/1‑149 (MQ=255)
                                      gcCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCg                                                                                                                 <  1:1889069/150‑1 (MQ=255)
                                         gTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTT‑TTTGTCTTCCGCAGCGGGGTTCGCTCCCCTTATTTGGCTCGccc                                                                                                              >  2:2567534/1‑149 (MQ=255)
                                          tCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTT‑TTTTGCTTCCGCAGCCGGCTGCCCCCCCCTTATTTGcccccccct                                                                                                             >  1:2834194/1‑143 (MQ=255)
                                           cAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTT‑TTTTGGTTCCGCAGCGGGGTGCGCCCCCCTTATGTGGCTCGcccca                                                                                                            >  2:1684741/1‑147 (MQ=255)
                                            aGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCatat                                                                                                           <  2:3131316/150‑1 (MQ=255)
                                            aGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCatat                                                                                                           <  1:3246998/150‑1 (MQ=255)
                                            aGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTT‑TTTTGCTTCTGCAGCGGGGTGTGCTCCCCTCATTTGGCCCGCCatat                                                                                                           >  2:231752/1‑150 (MQ=255)
                                            aGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTG‑TTTTGCTTCAGCAGCGGGGTGCGCTCCCCTTATGTGCCTCGCCatat                                                                                                           >  1:2682798/1‑150 (MQ=255)
                                              cggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTT‑TTTGGTTTCACCA‑CGGGGTGCGCTCCCTTTATTTGTCTCCCCCTATaa                                                                                                         >  2:2743471/1‑150 (MQ=255)
                                               ggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTT‑TTTTGCTTCAGCAGCGGGGTGCGCTCCCCTTATGTGGCTCGCCCTATaaa                                                                                                        >  1:2960553/1‑150 (MQ=255)
                                               ggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑CTTTT‑TTTTGGTTTTTCAGCCGGGTTCGCTCCCCTTATTTGGCCCGCCCTATaaa                                                                                                        >  1:1193792/1‑150 (MQ=255)
                                                gcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAAt                                                                                                       <  1:3995665/150‑1 (MQ=255)
                                                  gATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTT‑TTTGGCTTCCGCAGCGGGTTGCGCTCCCCTAATGTGGCTCGCCCTATTAAATg                                                                                                     >  1:4508612/1‑150 (MQ=255)
                                                  gATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTg                                                                                                     <  1:86338/150‑1 (MQ=255)
                                                   aTGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTT‑TTTTGCTTCAGCAGCGGGGTGGGCCCCCCTTATTTGGCTCCCCCCATaaaatta                                                                                                    >  2:892950/1‑146 (MQ=255)
                                                    tGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTT‑TTTTGGTTCTGCAGCGGGGTTCGCTCCCCTTATTTGCCTCGCCCTATAAATTTaa                                                                                                   >  1:3563933/1‑150 (MQ=255)
                                                      cAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATg                                                                                                 <  2:4958816/150‑1 (MQ=255)
                                                       aaaaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTTGCCTCAGCAGCGGGGTGCGCCCCCTTTATGTGCCTCGCCCTATAAATTGAATgg                                                                                                >  1:448399/1‑150 (MQ=255)
                                                       aaaaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATgg                                                                                                <  2:3998430/150‑1 (MQ=255)
                                                       aaaaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGGTGCGCTCCCCTTATGTGCCTCCCCCTATAAAATTAATgg                                                                                                >  1:260928/1‑150 (MQ=255)
                                                         aaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTT‑TTTTTCTTCATCAGCGGGGTTGGCCCCCTTTATTTGCCTCCCCCTTTAAAATTAAtaggg                                                                                              >  2:1669335/1‑146 (MQ=255)
                                                          aaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGc                                                                                             <  1:799648/150‑1 (MQ=255)
                                                           aCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCa                                                                                            <  1:611122/150‑1 (MQ=255)
                                                                        gCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTT‑TTTTGCTTCCGCAGCGGGGTGCGCTCCCCTTATGTGGCCCGCCCTATTAATTTAATGGTGGAGGGGGGGC‑GC‑Agg                                                                             >  2:2925990/1‑150 (MQ=255)
                                                                          cGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGGTGCGCCCCCCTAATTTGGCTCCCCCCATAAATTGGAAGGTGCCAGGGGGGC‑GC‑Cgggg                                                                           >  2:405810/1‑150 (MQ=255)
                                                                           gATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTT‑TTTTTCTTCAGCGTCGGGGTGCGCCCCCCTAATTGGCCCCGCCCTCTAAATTGAATTGTGGAGGGAGCGG‑GC‑Cggggg                                                                          >  2:4204160/1‑150 (MQ=255)
                                                                           gATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTT‑TTTTGGTTTAGCCGCGGGGTGCGCTCCCCTTATGTGCCTCCCCCTATAAATTTAATTGGGGAGGGGGGGC‑GC‑Aggggg                                                                          >  2:720800/1‑150 (MQ=255)
                                                                              gcgcACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTTGCTTTAGCCGCGGGGTGCGCTCCCCTTATGTTCCTCGCCATATAAATTTTATGGGGCCGGGGGGGC‑GC‑Cgggggggg                                                                       >  2:2879319/1‑150 (MQ=255)
                                                                                    tATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGC‑GC‑AGGGGGCGGCCAATc                                                                 <  2:4136136/150‑1 (MQ=255)
                                                                                     aTCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTTGTTTCAGCAGCGGGGTGCGGTCCCCTAATTTGGCCCGCCCTCTTAATTTGATTGTGGTGGGGGCGG‑CC‑CGGGGGCGGcccaacc                                                                >  2:4520793/1‑145 (MQ=255)
                                                                                         ttCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTT‑TTTTGGTTCAGCAGCGGGGTGTGCTCCCCTTATTTGGCTCTCCCATTAAAATTTATTGTGCAGGGGGCGC‑GCAAGGGGGGGGCCCA‑cgccgc                                                            >  2:3387596/1‑150 (MQ=255)
                                                                                           cGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGC‑GC‑AGGGGGCGGCCAATcgccgccg                                                          <  1:2419450/150‑1 (MQ=255)
                                                                                              cTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTT‑TTTTGCCTCCGCCGCGGGGTGCGCCCCCTTTATATGCCCCGCCATCTTAATTTTAATGTGCGGGGAGAGC‑GCGGGGGGGGGGCCAACCcccgccccc                                                        >  2:4524548/1‑150 (MQ=255)
                                                                                              cTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑ATTTT‑TTTCGCTTCACCAGCGGGGTGTGCCCCCTTTTTGTGGCGCGCCCTCTTAAATGTGTGGTGCAGGGGGGGC‑GC‑AGGGGGCGGCCCACCGCCGCCGccc                                                       >  2:2040284/1‑150 (MQ=255)
                                                                                                ccgccgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTT‑TTTTGCTTCAGCCGCGGGGTGGGCCCCCCTCATTTGTCTCTCCCTATAAAATTAATGGGGGCGGGGGGGCGGC‑GGGGGGGGGCCCA‑CCCCCCCGccccc                                                     >  2:1159000/1‑150 (MQ=255)
                                                                                                ccgccgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGC‑GC‑AGGGGGCGGCCAATCGCCGCCGccccc                                                     <  1:2217851/150‑1 (MQ=255)
                                                                                                 cgccgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGC‑GC‑AGGGGGCGGCCAATCGCCGCCGCCCCct                                                    <  1:1697032/150‑1 (MQ=255)
                                                                                                    cgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGC‑GC‑AGGGGGCGGCCAATCGCCGCCGCCCCctgc‑t                                                <  2:3777528/150‑1 (MQ=255)
                                                                                                    cgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGC‑GC‑AGGGGGCGGCCAATCGCCGCCGCCCCctgc‑t                                                <  1:2429160/150‑1 (MQ=255)
                                                                                                            ttGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGC‑GC‑AGGGGGCGGCCAATCGCCGCCGCCCCCTGC‑TGTCCCGGc                                        <  2:1384665/150‑1 (MQ=255)
                                                                                                            ttGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGC‑GC‑AGGGGGCGGCCAATCGCCGCCGCCCCCTGC‑TGTCCCGGc                                        <  1:4281145/150‑1 (MQ=255)
                                                                                                               cAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCTGCAAAGGGGTGCGGGCCCCTTATGGGGCTCGGCATATTATTTTGGTGGTTCAGGGGGGGC‑GC‑GGGGGGGGGCCAAACCGCGCCCCCCCCCGCGTGTCCGGGcgt                                      >  2:3541258/1‑148 (MQ=255)
                                                                                                                aGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTT‑TTTTGCTTTTGCAGCGGGGTGGGCTCCCCTTATGTGGCCCCCCCTATTAAATGGATGGTGCGGGGGGCGC‑GC‑CGGGGGGGGGCCATCGCCGCCGCCCCCCGC‑TGTCCCgggcctc                                    >  2:1835596/1‑145 (MQ=255)
                                                                                                                aGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGC‑GC‑AGGGGGCGGCCAATCGCCGCCGCCCCCTGC‑TGTCCCGGCCTTc                                    <  1:2516939/150‑1 (MQ=255)
                                                                                                                 ggAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGC‑GC‑AGGGGGCGGCCAATCGCCGCCGCCCCCTGC‑TGTCCCGGCCTTCg                                   <  2:4158070/150‑1 (MQ=255)
                                                                                                                           gggACTTAGCCTGCGGC‑GG‑GTTTT‑TTTGGCTTCAGCAGCGGGGTGCGCGCCCCCTATTGGCCTCGCCCTCTAAAATGAAACGCGCAGCGGGCGC‑GC‑AGGGGGCGGCCCATCGCCGCCCCCCCCCGC‑TGTCCCCGCCCTCGGGGGACGcgt                         >  2:1542031/1‑148 (MQ=255)
                                                                                                                              actaggCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCTATATAAATTGAATGGTGCAGGGAGCGC‑GC‑AGGGGGCGGCCAATCGCCGCCGCCCCCTGC‑TGTCCCGGCCTTCGGGGAACGCTTCAg                      <  2:4062273/145‑1 (MQ=255)
                                                                                                                                ttAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGC‑GC‑AGGGGGCGGCCAATCGCCGCCGCCCCCTGC‑TGTCCCGGCCTTCGGGGAACGCTTCAGCg                    <  1:5179599/150‑1 (MQ=255)
                                                                                                                                   gCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGC‑GC‑AGGGGGCGGCCAATCGCCGCCGCCCCCTGC‑TGTCCCGGCCTTCGGGGAACGCTTCAGCGAtt                 <  2:367983/150‑1 (MQ=255)
                                                                                                                                         ggc‑gg‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGC‑GC‑AGGGGGCGGCCAATCGCCGCCGCCCCCTGC‑TGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACg           >  1:2044242/1‑150 (MQ=255)
                                                                                                                                             gg‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGC‑GC‑AGGGGGCGGCCAATCGCCGCCGCCCCCTGC‑TGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGcca        >  1:623771/1‑150 (MQ=255)
                                                                                                                                             gg‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGC‑GC‑AGGGGGCGGCCAATCGCCGCCGCCCCCTGC‑TGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGcca        >  2:4663384/1‑150 (MQ=255)
                                                                                                                                              g‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGC‑GC‑AGGGGGCGGCCAATCGCCGCCGCCCCCTGC‑TGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccac       <  2:1082398/150‑1 (MQ=255)
                                                                                                                                              g‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGC‑GC‑AGGGGGCGGCCAATCGCCGCCGCCCCCTGC‑TGTCCCGGCCTTCGGGGAACGCTTCAACGATTTTGACGccac       >  2:4298130/1‑150 (MQ=255)
                                                                                                                                                ttttG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGC‑GC‑AGGGGGCGGCCAATCGCCGCCGCCCCCTGC‑TGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccacc      >  1:4902698/1‑150 (MQ=255)
                                                                                                                                                 tttg‑tttgGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGC‑GC‑AGGGGGCGGCCAATCGCCGCCGCCCCCTGC‑TGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccacca     >  2:1715720/1‑150 (MQ=255)
                                                                                                                                                  ttg‑tttgGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGC‑GC‑AGGGGGCGGCCAATCGCCGCCGCCCCCTGC‑TGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCaa    >  1:950243/1‑150 (MQ=255)
                                                                                                                                                    g‑tttgGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGC‑GC‑AGGGGGCGGCCAATCGCCGCCGCCCCCTGC‑TGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAaca  >  2:3894124/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                         
AGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTG‑TTTGGCTTCAGCAGCGGGTTGCGCTCCCTTAATGTGCCTCGCCATATAAATTGAATGGTGCAGGGAGCGC‑GC‑AGGGGGCGGCCAATCGCCGCCGCCCCCTGC‑TGTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACA  >  AP012306/934908‑935203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑