Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG42.6% 479.5 / 92.0 312intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
Reads supporting (aligned to +/- strand):  ref base T (71/108);  new base G (133/0);  total (204/108)
Fisher's exact test for biased strand distribution p-value = 8.67e-36
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 348 total aligned reads displayed.

GCTGGGGATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGT  >  AP012306/1091843‑1092141
                                                                                                                                                     |                                                                                                                                                      
gCTGGGGATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGt                                                                                                                                                        <  2:3036036/150‑1 (MQ=255)
  tGGGGATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGttt                                                                                                                                                      <  2:4855690/150‑1 (MQ=255)
    gggATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGttttt                                                                                                                                                    <  2:1191795/150‑1 (MQ=255)
    gggATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGttttt                                                                                                                                                    >  1:1508338/1‑150 (MQ=255)
      gATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGgtttttt                                                                                                                                                  >  1:3813297/1‑150 (MQ=255)
      gATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGgtttttt                                                                                                                                                  >  1:4912145/1‑150 (MQ=255)
       aTGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTa                                                                                                                                                 >  1:3163625/1‑150 (MQ=255)
         gACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATg                                                                                                                                               >  1:1352356/1‑150 (MQ=255)
           cGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATtgg                                                                                                                                             >  1:4025476/1‑150 (MQ=255)
           cGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATggg                                                                                                                                             >  2:2880493/1‑150 (MQ=255)
             ttATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTTTGGGCa                                                                                                                                           >  2:145933/1‑150 (MQ=255)
               aTTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTTTGGGCACg                                                                                                                                         >  2:318340/1‑150 (MQ=255)
               aTTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACg                                                                                                                                         >  2:104561/1‑150 (MQ=255)
                   ctgctgTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGc                                                                                                                                     <  1:1143095/150‑1 (MQ=255)
                      ctgTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCggg                                                                                                                                  <  1:2538148/150‑1 (MQ=255)
                      ctgTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCggg                                                                                                                                  <  1:2851972/150‑1 (MQ=255)
                       tgTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCgggg                                                                                                                                 >  1:3927892/1‑150 (MQ=255)
                       tgTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGATTTTTTATTGGCACCGTGCgggg                                                                                                                                 >  1:3448672/1‑150 (MQ=255)
                           cccGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTTTTGGGACGGTGCGGGGGGAg                                                                                                                             >  2:1305039/1‑150 (MQ=255)
                           cccGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTTTGGGGACGGTGCgggggggg                                                                                                                             >  2:4769784/1‑146 (MQ=255)
                             cGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTTTGGGCACGGGGCGGGGTGAggt                                                                                                                           >  2:599435/1‑148 (MQ=255)
                               ttCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATTGGCACGGTGCGGGGGGGGTTGt                                                                                                                         >  2:4309679/1‑150 (MQ=255)
                                tctcGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTc                                                                                                                        <  1:4535407/150‑1 (MQ=255)
                                tctcGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGTTGTc                                                                                                                        >  2:457763/1‑150 (MQ=255)
                                    gcgTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGAt                                                                                                                    <  1:3554172/150‑1 (MQ=255)
                                    gcgTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGAt                                                                                                                    <  1:2089374/150‑1 (MQ=255)
                                    gcgTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTTTGGGCACGGGGCGGGGGGAGGTGTCGGAt                                                                                                                    >  2:3960111/1‑150 (MQ=255)
                                       tCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATgcg                                                                                                                 <  1:2935641/150‑1 (MQ=255)
                                       tCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGGGCGGGGGGAGGTGTCGGATgcg                                                                                                                 >  2:2734153/1‑150 (MQ=255)
                                         tGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGcgct                                                                                                               >  1:4377756/1‑150 (MQ=255)
                                          gATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATTGGGACGGGGCGGGGGGAGTTGTCGGGTGcgctt                                                                                                              >  2:413608/1‑150 (MQ=255)
                                           aTACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGcgcttc                                                                                                             <  1:1691685/150‑1 (MQ=255)
                                            tACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGcgcttcg                                                                                                            <  1:3288549/150‑1 (MQ=255)
                                              cacaTCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGcgcttcgct                                                                                                          <  1:2743136/150‑1 (MQ=255)
                                                caTCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTTTTGGCACGGTGCGGGGGGGGTTGTCGGAAGcgcctcgcttt                                                                                                        >  2:3767623/1‑149 (MQ=255)
                                                 aTCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGGACGGTGGGGGGGGAGTTGGCGGGTGGGGTTCGGTTAt                                                                                                       >  2:3415986/1‑150 (MQ=255)
                                                  tCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATc                                                                                                      >  2:2400307/1‑150 (MQ=255)
                                                  tCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATc                                                                                                      <  1:5027574/150‑1 (MQ=255)
                                                    tGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCg                                                                                                    <  2:4015317/150‑1 (MQ=255)
                                                     ggAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGa                                                                                                   <  2:1851014/150‑1 (MQ=255)
                                                      gAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGGACGGGGCGGGGGGAGGTGTCGGGTGCGCGTCGGTTTTTCGAc                                                                                                  >  2:3613362/1‑150 (MQ=255)
                                                         cGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTa                                                                                               <  1:3721552/150‑1 (MQ=255)
                                                          gTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTAc                                                                                              >  1:1009221/1‑150 (MQ=255)
                                                          gTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTAc                                                                                              >  1:637039/1‑150 (MQ=255)
                                                           tACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATTGGGCCGGCGCGGGGGGGGGTGTGGGGTGCGCCTTTCTTATCCGaacccca                                                                                             >  2:5226579/1‑144 (MQ=255)
                                                            aCCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAg                                                                                            <  1:4858728/150‑1 (MQ=255)
                                                              cGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGGGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAggg                                                                                          >  2:2149318/1‑150 (MQ=255)
                                                               ggTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAgggg                                                                                         >  2:4362420/1‑150 (MQ=255)
                                                                   gAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGggagga                                                                                     >  1:3979837/1‑150 (MQ=255)
                                                                    aGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGat                                                                                    <  1:1078506/150‑1 (MQ=255)
                                                                     gTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGata                                                                                   <  2:2811514/150‑1 (MQ=255)
                                                                        tCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTg                                                                               >  2:5066028/1‑150 (MQ=255)
                                                                         cTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGt                                                                              <  1:229585/150‑1 (MQ=255)
                                                                          tGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTa                                                                             >  1:2799485/1‑150 (MQ=255)
                                                                           gACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGGGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGGGGATA‑TTGTAg                                                                            >  1:4250473/1‑150 (MQ=255)
                                                                             cacGTAAGTACCGGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGc                                                                          <  2:4930761/150‑1 (MQ=255)
                                                                                      aCCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGGGGGTA‑TTGTAGGCCCGGGAAAc                                                                 >  2:4632976/1‑150 (MQ=255)
                                                                                      aCCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAAc                                                                 >  1:3666917/1‑150 (MQ=255)
                                                                                        cAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGGGGGTA‑TTGTAGGCCCGGGAAACgt                                                               >  2:2952159/1‑150 (MQ=255)
                                                                                         aGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACgtg                                                              >  1:1616039/1‑150 (MQ=255)
                                                                                         aGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGGAAAAgtg                                                              >  2:2657383/1‑150 (MQ=255)
                                                                                           cTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACgtggt                                                            >  1:4659201/1‑150 (MQ=255)
                                                                                             ggTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACGTGGTGc                                                          >  1:1962366/1‑150 (MQ=255)
                                                                                              gTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACGTGGTGcc                                                         <  1:984214/150‑1 (MQ=255)
                                                                                               tGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACGTGGTGCCa                                                        >  1:2228619/1‑150 (MQ=255)
                                                                                                gcgcgcTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACGTGGTGCCAt                                                       <  1:4207437/150‑1 (MQ=255)
                                                                                                 cgcgcTTGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACGTGGTGCCATc                                                      <  2:4984571/150‑1 (MQ=255)
                                                                                                  gcgcTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACGTGGTGCCATCg                                                     <  2:1719230/150‑1 (MQ=255)
                                                                                                   cgcTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGGTA‑TTGTAGGCCCGGTAAACGTGGTGCCATCgg                                                    >  2:3668159/1‑150 (MQ=255)
                                                                                                    gcTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACGTGGTGCCATCggg                                                   >  1:5116005/1‑150 (MQ=255)
                                                                                                     cTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACGTGGTGCCATCGGGc                                                  <  2:790069/150‑1 (MQ=255)
                                                                                                       cGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACGTGGTGCCATCGGGCaa                                                <  1:583853/150‑1 (MQ=255)
                                                                                                       cGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGGTA‑TTGTAGGCCCGGTAAACGTGGTGCCATCGGGCaa                                                >  2:2307411/1‑150 (MQ=255)
                                                                                                        gTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACGTGGTGCCATCGGGCaaa                                               <  2:3295435/150‑1 (MQ=255)
                                                                                                         tCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGGACGGTGCGGGGGGGGTTGTCGGATGCGGCTCGCTTATCCGCCCTACAGGGGAGGGTA‑TTTTAGGACCGGGAAACATGGGGCCATCGGGCcaaa                                              >  2:4217019/1‑150 (MQ=255)
                                                                                                          cACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAAt                                             >  1:1897309/1‑150 (MQ=255)
                                                                                                          cACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAAt                                             >  1:3483907/1‑150 (MQ=255)
                                                                                                          cACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAAt                                             >  1:78464/1‑150 (MQ=255)
                                                                                                           aCTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAAtt                                            >  1:1808129/1‑150 (MQ=255)
                                                                                                             tAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTTTGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCCTCGCTTATTCCACCTACAGGGGGGGGTA‑TTTTAGGCCCGGGAAACGTGGGGGCATCGGGCCAAAATAg                                          >  2:1216002/1‑150 (MQ=255)
                                                                                                               aGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGGAAACGTGGTGCCATCGGGCAAAATTAGCt                                        >  2:156720/1‑150 (MQ=255)
                                                                                                                         aGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACa                              <  2:1655377/150‑1 (MQ=255)
                                                                                                                         aGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGGGGGTA‑TTGTAGGCCCGGTAAAAGTGGTGGCATCGGGGAAAATTAGCTCGGGGGAACa                              >  2:2562083/1‑150 (MQ=255)
                                                                                                                            tCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGGTA‑TTGTAGGCCCGGGAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGt                           >  1:1429344/1‑150 (MQ=255)
                                                                                                                            tCACATAGACCCTGCTTCGGCGGGGGTTTTTTTTGGGGACGGGGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATTCGACCTACAGGGGGGGGTA‑TTGTAGGCCCGGGAAACGTGGGGGCCTCGGGCAAAATTAGCTCGGGGGAACAggg                           >  2:2381533/1‑149 (MQ=255)
                                                                                                                             cacaTAGACCCTGCTTCGGCGGGGTTTTTTTATGGGGACGGGGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGGTA‑TTGTAGGCCCGGTAAACGTGGGGCCATCGGGCAAAATTAGCTCGGAGTAACAgggt                          >  2:1924095/1‑148 (MQ=255)
                                                                                                                             cacaTAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGtt                          <  1:895383/150‑1 (MQ=255)
                                                                                                                             cacaTAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGtt                          <  1:2559443/150‑1 (MQ=255)
                                                                                                                              acaTAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGttt                         <  2:1143450/150‑1 (MQ=255)
                                                                                                                              acaTAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGttt                         >  1:269759/1‑150 (MQ=255)
                                                                                                                              acaTAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGGGCGGGGGGAGGTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGGGGGTA‑TTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGGGGAACAGGttt                         >  2:1778027/1‑150 (MQ=255)
                                                                                                                                aTAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACGTGGTGCTATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTg                       <  2:2167771/150‑1 (MQ=255)
                                                                                                                                aTAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGGGCGGGGGGAGTTGTCGGGTGCGCTTCGCTTATCCGACCTACAGGGGGGGATA‑TTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTg                       >  2:760574/1‑150 (MQ=255)
                                                                                                                                  aGACCCTGCTTCGGCGGGGGTTTTTTTTTGGGACGGGGCGGGGGGGGGTGTCGGGTGCGCCTCGCTTTTTCCACCTACAGGGGGGGGTATTTTTGGGCCCGGGAAAGGGGGGGCATCCGGGGAAAATTACTCCGGGGAAAAAGGttttat                      >  2:3903666/1‑148 (MQ=255)
                                                                                                                                       cTGCTTCGGCGGGGGTTTTTTTTGGGCACGGTGCGGGGGGAGTTGTCGGGTGCGCTTCGCTTATCCGACCTACAGGGGGGGGTA‑TTGTAGGGCCGGTAAACGTGGTGCCATCCGGCAAAATTAGCTCGGAGGAACAGGGTTTGATTAttt                >  2:3727678/1‑150 (MQ=255)
                                                                                                                                            tCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGa           <  1:2400307/150‑1 (MQ=255)
                                                                                                                                              ggcggGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAg         <  2:2291982/150‑1 (MQ=255)
                                                                                                                                              ggcggGGTTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAg         >  2:694199/1‑150 (MQ=255)
                                                                                                                                                 ggggTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGa      >  1:3008886/1‑150 (MQ=255)
                                                                                                                                                   ggTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGAtg    <  1:4332210/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GCTGGGGATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATA‑TTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGT  >  AP012306/1091843‑1092141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑