Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,372,7010TG25.0% 216.3 / 12.2 96noncoding (54/76 nt)gltUtRNA‑Glu
Reads supporting (aligned to +/- strand):  ref base T (3/69);  new base G (24/0);  total (27/69)
Fisher's exact test for biased strand distribution p-value = 1.12e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 164 total aligned reads displayed.

CACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTA  >  AP012306/3372553‑3372757
                                                                                                                                                      |                                                        
cACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AAGGGtt                                                         >  2:4747934/1‑150 (MQ=17)
 aCCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAACAAGGGGtt                                                         >  1:1662700/1‑150 (MQ=17)
 aCCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTc                                                        <  2:3273280/150‑1 (MQ=32)
 aCCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGGTc                                                        >  1:1024173/1‑150 (MQ=17)
  cctcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCg                                                       >  2:1467654/1‑150 (MQ=32)
  cctcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAA‑AGGGGtttg                                                       >  2:4610504/1‑148 (MQ=17)
   ctcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAA‑AGGGGCTCgc                                                      >  2:4104371/1‑149 (MQ=17)
   ctcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGAAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGGTCGa                                                      >  1:424438/1‑150 (MQ=17)
     ccttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCTTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAAt                                                    <  2:140326/150‑1 (MQ=17)
     ccttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGGAAC‑AGGGGGTCGAAt                                                    >  2:4335906/1‑150 (MQ=17)
      cttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGCAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCCCCCTTTCACGGC‑GGTAAC‑ACGGGGTCGAATc                                                   >  1:4174164/1‑150 (MQ=255)
      cttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AAGGGGTCGAATc                                                   >  2:39115/1‑150 (MQ=17)
       ttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AAGGGGTCGAATcc                                                  >  1:4173068/1‑150 (MQ=17)
        taccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATccc                                                 >  1:3204239/1‑150 (MQ=32)
        taccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATccc                                                 <  1:481441/150‑1 (MQ=32)
        taccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAA‑AGGGGGGCGAATccc                                                 >  2:2475204/1‑150 (MQ=12)
         accttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATcccc                                                <  2:725656/150‑1 (MQ=32)
         accttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑ACGGGCTCGTATcccc                                                >  1:2835311/1‑150 (MQ=12)
          ccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCt                                               <  2:325192/150‑1 (MQ=32)
          ccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGGTCGAATccccc                                               >  1:2675598/1‑149 (MQ=17)
          ccctaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCt                                               <  1:1962465/147‑1 (MQ=17)
           cttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AAGGGTTCGAATccccca                                              >  1:2301460/1‑148 (MQ=17)
           cttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGGAAA‑AAGGGGTCGAATccccca                                              >  2:3809844/1‑148 (MQ=255)
            ttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAA‑AGGGGGTCGAATCCCCTan                                             >  1:2120694/1‑149 (MQ=17)
             taAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGGTCGAATcccccaag                                            >  1:1367155/1‑146 (MQ=17)
             taAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGGTCGAATCCCCTAgg                                            >  1:4448926/1‑150 (MQ=17)
             taAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGGTCGAATCCCCTAgg                                            >  1:435467/1‑150 (MQ=17)
              aaaGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGGTACA‑AGGGGTTCGAATCCCCTggg                                            >  1:5267455/1‑150 (MQ=11)
              aaaGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAggg                                           <  1:2981860/150‑1 (MQ=32)
              aaaGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAggg                                           <  1:547567/150‑1 (MQ=32)
              aaaGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAggg                                           <  2:3491490/150‑1 (MQ=32)
              aaaGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAggg                                           <  2:3219030/150‑1 (MQ=32)
              aaaGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAA‑AGGGGGTCGAATCCCCTAggg                                           >  1:1969566/1‑150 (MQ=17)
                agaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAA‑AAGGGGTCGAATCCCCTAAGGGa                                         >  1:1618209/1‑150 (MQ=255)
                 gaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGAc                                        <  1:765523/150‑1 (MQ=32)
                  aagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACg                                       <  2:1748572/150‑1 (MQ=32)
                  aagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AAGGGGTCGAATCCCCCCGGGGACg                                       >  1:1000811/1‑150 (MQ=255)
                   agCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACGc                                      <  2:262477/150‑1 (MQ=32)
                        tCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACGCCACtt                                 <  2:5158869/150‑1 (MQ=32)
                        tCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGGTCGAATCCCCCAGGGGGCGCCAcct                                 >  1:1322758/1‑148 (MQ=255)
                         cTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACGCCACTTg                                <  1:240153/150‑1 (MQ=32)
                         cTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGGGTGAATCCCCCAGGGGGCGccccctt                                >  2:1506096/1‑145 (MQ=11)
                         cTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAA‑AAGGGGTCGGATCCCCCAGGGGACGccccctt                                >  2:5053499/1‑145 (MQ=11)
                           ttGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AAGGGGTCGAATCCCCTAGGGGGCGCCCCTTTCt                              >  1:863119/1‑150 (MQ=11)
                            tGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTg                             <  2:2314424/150‑1 (MQ=32)
                             gCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTCGGGGACGCCACTTGCTgg                            >  1:1105403/1‑150 (MQ=17)
                             gCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTgg                            <  1:4535173/150‑1 (MQ=32)
                             gCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTgg                            <  1:276708/150‑1 (MQ=32)
                             gCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAAACCCCCAAGGGGCGCCACCTGCTgg                            >  1:2384290/1‑150 (MQ=11)
                             gCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGGAAC‑AAGGGTTCGAATCCCCTAAGGGAAGCCACTTGCTgg                            >  2:3397494/1‑150 (MQ=255)
                              cAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGt                           <  1:1197700/150‑1 (MQ=32)
                              cAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AAGGGTTCGAATACCCTAAGTGGCGCCCATTGCTggg                           >  1:652663/1‑149 (MQ=11)
                              cAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AAGGGGTCGCATCCCCCCAGGGGCGCCCCTTTCTggg                           >  1:1473323/1‑149 (MQ=11)
                               aGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACGCCCCTTGCTGGtt                          >  1:4661528/1‑150 (MQ=17)
                                gTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGGTCGAATCCCCTAGGGGACGCCACTTTCTGGttt                         >  2:1997068/1‑150 (MQ=17)
                                gTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGGTCGAATCCCCTAGGGGACGCCACTTGCTGGttt                         >  1:3655686/1‑150 (MQ=17)
                                 tGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACCCCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTtg                        <  2:406331/150‑1 (MQ=17)
                                 tGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTtg                        <  2:3857407/150‑1 (MQ=32)
                                 tGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGGTCGAATCCCCTAGGGGACGCCACTTGCTGGTTtg                        >  1:2101777/1‑150 (MQ=17)
                                 tGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGCAAC‑AGGGGTTCGCATCCCCTAGGGGAGGCCACTTGCTGGTTtg                        >  2:2271624/1‑150 (MQ=12)
                                  gCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGGTCGAATCCCCTTGGGGACGCCACTTGCTGGttttt                       >  1:3965430/1‑148 (MQ=12)
                                    tCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGGTCGAATCCCCCAGGGGGCGCCACTTGCTGGGTTgtga                     >  2:527082/1‑150 (MQ=255)
                                    tCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AAGGGGTCGAATCCCCTAGGGGGGGCCCCCTGCCGGGTTgtga                     >  2:2816082/1‑150 (MQ=11)
                                     cacacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgag                    <  2:1542744/150‑1 (MQ=32)
                                     cacacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AAGGGTTCGGATCCCCTAGGGGGCGCCACTTGCTGGTTTgtgag                    >  1:2065211/1‑150 (MQ=12)
                                      acacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgagt                   <  1:4928706/150‑1 (MQ=32)
                                      acacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTgtgagt                   <  1:5190903/150‑1 (MQ=32)
                                       cacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAA‑ACAGGGTCGAATACCCTCTGGGGAGCCACCTGCTCGGTTttgggtg                  >  1:2486031/1‑150 (MQ=11)
                                       cacaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGGAAC‑AGGGGGTGGGAACCCCCAGGGGCCGCCACCTGCTGGTTtgtggggg                  >  2:1886758/1‑146 (MQ=11)
                                        acaGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGGTCGAATCCCCTAGGGGACGCCACTTGCTGGGTTgtgagtgg                 >  1:1132050/1‑149 (MQ=17)
                                         caGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGGTCGAATCCCCTAGGGGACGCCCCTTGCTGGGTTgtgagtgga                >  1:3391671/1‑148 (MQ=255)
                                          aGATTGTCTGATAGGAAGTTGAAAGCAAGGGGTCCTGGGAAGCAGACGGGTACCTCACCTTCGTCTAGATGCCCTGGACTGCGCCCTTTCACGGG‑GGTAAC‑AGGGGGTCGAATTCCCTTGGGGACCACGGTTGCTGGTTTGTGAGTGaaa               <  2:240724/150‑1 (MQ=2)
                                          aGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGaaa               <  1:1824963/150‑1 (MQ=32)
                                          aGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AAGGGGTCGAATCCCCCAGGGGACGCCACTTGCTGGTTTGTGAGTGaaa               >  1:968925/1‑150 (MQ=12)
                                           gATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAg              <  1:655139/150‑1 (MQ=32)
                                           gATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGGTCGAATCCCCTAGGGGGCGCCCCTTGCTGGGTTTTGAGTGGAAg              >  2:3103546/1‑150 (MQ=11)
                                            aTTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGt             <  1:5119613/150‑1 (MQ=32)
                                            aTTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGt             <  1:4281921/150‑1 (MQ=32)
                                            aTTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGt             <  2:2339997/150‑1 (MQ=32)
                                            aTTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGGTCGAATTCCCTATGGGGCGCCACCTGCTGGTTTGTGAGTGaaaat             >  1:3730329/1‑148 (MQ=11)
                                            aTTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGGTCGAAACCCCCAGGGGACGCCCCTTGCTGGGTTGTGAGTGAAAga             >  2:4955109/1‑149 (MQ=11)
                                            aTTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAA‑CGGGGGCTGTATACCCTCAGGGAGGCCACGCGCAGGTTTgtgagttagaga             >  2:3582431/1‑144 (MQ=11)
                                             ttGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTc            <  1:2563723/150‑1 (MQ=32)
                                             ttGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTc            <  2:4565151/150‑1 (MQ=32)
                                              tGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCa           <  2:3987678/150‑1 (MQ=32)
                                               gTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCAc          <  1:956521/150‑1 (MQ=32)
                                               gTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AAGGGGTCGAATCCCCCCGGGGACGCCCCTTGCTGGTTTTTGAGTGAAAGGCAc          >  2:658299/1‑150 (MQ=11)
                                               gTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AAGGGGTCGAATCCCCCAAGGGGAGCCACTTGCTGGGTTGTGAGTGAAAGGCAc          >  1:5089662/1‑150 (MQ=11)
                                                 cTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGGTCGAATCCCCTCGGGGACGCCACTTGCTGGTTTGTGAGTGAAAAACAccc        >  1:4156675/1‑149 (MQ=255)
                                                 cTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑ACGGGGTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGGAAGTCACCt        >  1:394529/1‑150 (MQ=12)
                                                 cTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AAGGGGTCGAATCCCCCAGGGGACGCCACCTGCTGGTTTGTGAGGGAAAGTCACCt        >  1:4997085/1‑150 (MQ=11)
                                                  tGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGGAAA‑AGGGGGTCGAATGCCCCAAGGGGCGCCACTTGCTGGTTTGTGAGGGAAAGGCACCTg       >  2:1588916/1‑150 (MQ=11)
                                                   gATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGc      <  1:3021321/150‑1 (MQ=32)
                                                    aTAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGcc     <  1:3206303/150‑1 (MQ=32)
                                                    aTAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AAGGGGTCGAATCCCCTAGGGGGCGCCACTTGCTGGGTTGTGAGTGAAAGTCACCTGcc     >  1:3481403/1‑150 (MQ=255)
                                                    aTAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AAGGGGTCGAAACCCCCCGGGGGCGCCCCCTGCTGGGTTGTGAGGGAAAAGCACCCGcc     >  2:1171981/1‑150 (MQ=11)
                                                     tAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCt    <  2:363278/150‑1 (MQ=32)
                                                      aGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AAGGGGTCGAAACCCCCAGGGGGGGCCACTTTCTGGTTTGTGAGTGGAAGGCACCCGCCtt   >  2:3413189/1‑150 (MQ=11)
                                                       ggAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGGTCGAATCCCCTAGGGGGCGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCttt  >  1:1574245/1‑149 (MQ=14)
                                                       ggAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AAGGGGTCGAATCCCCCAGGGGGCGCCCCCTGCTGGGTTGTGAGTGAAAGTCACCCGCCttt  >  2:1168141/1‑149 (MQ=11)
                                                                                                                                                      |                                                        
CACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGC‑GGTAAC‑AGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTA  >  AP012306/3372553‑3372757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑