BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	1,430,401	C→G	E248Q (GAA→CAA)	ydhT ←	conserved protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	1,430,401	0	C	G	99.7%	1462.0 / ‑5.0	349	E248Q (GAA→CAA)	ydhT	conserved protein
Reads supporting (aligned to +/- strand): ref base C (0/1); new base G (151/197); total (151/198)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 354 total aligned reads displayed.

AGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGC  >  AP012306/1430254‑1430547

 ggCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAAt                                                                                                                                                 <  2:610673/150‑1 (MQ=255)

  gCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATg                                                                                                                                                <  2:961608/150‑1 (MQ=255)

   cGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATgg                                                                                                                                               >  1:1836749/1‑150 (MQ=255)

    gATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGc                                                                                                                                              <  1:4943133/150‑1 (MQ=255)

    gATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGc                                                                                                                                              >  1:2350742/1‑150 (MQ=255)

    gATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGc                                                                                                                                              <  1:2062249/150‑1 (MQ=255)

     aTGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCg                                                                                                                                             <  1:4576884/150‑1 (MQ=255)

      tGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGa                                                                                                                                            <  2:491698/150‑1 (MQ=255)

          tCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGgc                                                                                                                                        >  1:757515/1‑150 (MQ=255)

            gggCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTACATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGgcga                                                                                                                                      <  1:4191531/150‑1 (MQ=255)

             ggCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGgcgaa                                                                                                                                     <  1:1033036/150‑1 (MQ=255)

             ggCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGgcgaa                                                                                                                                     <  2:4472957/150‑1 (MQ=255)

               cGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGgcgaagc                                                                                                                                   <  1:1592420/150‑1 (MQ=255)

               cGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGgcgaagc                                                                                                                                   >  1:3906976/1‑150 (MQ=255)

                ggTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGgcgaagcg                                                                                                                                  >  2:5227827/1‑150 (MQ=255)

                ggTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGgcgaagcg                                                                                                                                  >  1:1262587/1‑150 (MQ=255)

                  tCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGgcgaagcgaa                                                                                                                                <  1:585036/150‑1 (MQ=255)

                    gTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAAct                                                                                                                              <  1:2144577/150‑1 (MQ=255)

                    gTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAAct                                                                                                                              <  2:4888223/150‑1 (MQ=255)

                      aaaGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAActct                                                                                                                            <  1:2472445/150‑1 (MQ=255)

                       aaGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCtt                                                                                                                           <  2:1212703/150‑1 (MQ=255)

                           cTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGc                                                                                                                       <  1:329929/150‑1 (MQ=255)

                             gCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCg                                                                                                                     >  2:4697813/1‑150 (MQ=255)

                              cATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGt                                                                                                                    <  1:4334025/150‑1 (MQ=255)

                               aTCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTc                                                                                                                   >  1:2918424/1‑150 (MQ=255)

                               aTCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTc                                                                                                                   <  1:3187648/150‑1 (MQ=255)

                                      gCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGatat                                                                                                            <  2:2453641/150‑1 (MQ=255)

                                      gCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGatat                                                                                                            <  1:3812136/150‑1 (MQ=255)

                                        tcgtcgGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGc                                                                                                          <  2:4977553/150‑1 (MQ=255)

                                         cgtcgGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGcc                                                                                                         >  1:276775/1‑150 (MQ=255)

                                          gtcgGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCa                                                                                                        >  1:865598/1‑150 (MQ=255)

                                          gtcgGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCa                                                                                                        >  1:4150176/1‑150 (MQ=255)

                                          gtcgGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCa                                                                                                        >  1:4391794/1‑150 (MQ=255)

                                            cgGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGt                                                                                                      <  2:4193199/150‑1 (MQ=255)

                                            cgGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGt                                                                                                      <  2:3738483/150‑1 (MQ=255)

                                             ggCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTg                                                                                                     >  1:1432570/1‑150 (MQ=255)

                                              gCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTgc                                                                                                    <  2:3852680/150‑1 (MQ=255)

                                              gCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTgc                                                                                                    <  1:2573160/150‑1 (MQ=255)

                                              gCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTgc                                                                                                    <  1:4789496/150‑1 (MQ=255)

                                               cGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTgcg                                                                                                   >  1:1176767/1‑150 (MQ=255)

                                                gTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTgcgg                                                                                                  <  1:2873071/150‑1 (MQ=255)

                                                gTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTgcgg                                                                                                  >  2:5037387/1‑150 (MQ=255)

                                                 tCTTTCGTTTGATTAAAGGAAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTgcggc                                                                                                 >  2:2241787/1‑150 (MQ=255)

                                                        tttGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTtcct                                                                                          >  2:1823769/1‑150 (MQ=255)

                                                         ttGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTtcctc                                                                                         >  2:1035979/1‑150 (MQ=255)

                                                         ttGATTAAAGGTAAAGCTACCAGCACTCAACAAACTGATGATGTTAATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTATCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCTGTCATGATATGCCAGTGCGGCGCTttctc                                                                                         <  2:112602/150‑1 (MQ=255)

                                                          tGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTtcctcc                                                                                        >  1:3259951/1‑150 (MQ=255)

                                                             ttAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTgg                                                                                     <  1:5240471/150‑1 (MQ=255)

                                                                aaGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGccc                                                                                  >  1:2017922/1‑150 (MQ=255)

                                                                     aaaGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGaa                                                                             >  1:4595279/1‑150 (MQ=255)

                                                                     aaaGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGaa                                                                             >  2:3253231/1‑150 (MQ=255)

                                                                      aaGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGaat                                                                            >  1:3495720/1‑150 (MQ=255)

                                                                               gCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTc                                                                   >  2:3300434/1‑150 (MQ=255)

                                                                                     aacaaACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCAc                                                             >  2:3681057/1‑150 (MQ=255)

                                                                                     aacaaACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCAc                                                             <  2:3408796/150‑1 (MQ=255)

                                                                                       caaACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGc                                                           >  2:3171613/1‑150 (MQ=255)

                                                                                         aaCTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGccc                                                         <  1:1743959/150‑1 (MQ=255)

                                                                                            tGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTg                                                      >  2:4642166/1‑150 (MQ=255)

                                                                                            tGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTg                                                      <  1:1378680/150‑1 (MQ=255)

                                                                                            tGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTg                                                      >  1:1644258/1‑150 (MQ=255)

                                                                                             gATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGc                                                     >  1:4366230/1‑150 (MQ=255)

                                                                                             gATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCCAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGc                                                     <  2:2125574/150‑1 (MQ=255)

                                                                                                gCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCa                                                  <  2:2085861/150‑1 (MQ=255)

                                                                                                  tGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACt                                                <  1:1014949/150‑1 (MQ=255)

                                                                                                     tCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTAcc                                             <  2:4017848/150‑1 (MQ=255)

                                                                                                      cATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCg                                            <  2:2802482/150‑1 (MQ=255)

                                                                                                             aCGATAGCGGATTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACCGCGAAGAGAACTCTTTCGCCGTCATGATATGCGAGTTCGGCGCTTCCTGCTGGGCCGAGATTAACTTTGTCCACCACCCCCATGCTCACTACCGTTGAAtt                                     >  2:3046073/1‑150 (MQ=255)

                                                                                                             aCGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAAtt                                     <  2:3925915/150‑1 (MQ=255)

                                                                                                              cGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAAttt                                    <  2:2876411/150‑1 (MQ=255)

                                                                                                               gATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAAtttt                                   <  1:5026515/150‑1 (MQ=255)

                                                                                                                  aGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCAc                                >  2:3450875/1‑150 (MQ=255)

                                                                                                                  aGCGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCAc                                <  2:165190/150‑1 (MQ=255)

                                                                                                                    cGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCg                              <  1:1548454/150‑1 (MQ=255)

                                                                                                                    cGGACTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCg                              >  1:662174/1‑150 (MQ=255)

                                                                                                                        cTCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTgg                          >  1:2218883/1‑150 (MQ=255)

                                                                                                                         tCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGc                         <  2:2985367/150‑1 (MQ=255)

                                                                                                                         tCCGGCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGc                         <  2:3540739/150‑1 (MQ=255)

                                                                                                                            ggCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAat                      <  2:721142/150‑1 (MQ=255)

                                                                                                                             gCTCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAatg                     <  1:4506137/150‑1 (MQ=255)

                                                                                                                               tctaaTATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAatgat                   <  2:2347353/147‑1 (MQ=255)

                                                                                                                               tCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAatgat                   >  1:4979154/1‑150 (MQ=255)

                                                                                                                               tCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAatgat                   <  1:3048506/150‑1 (MQ=255)

                                                                                                                               tCGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAatgat                   >  1:3405229/1‑150 (MQ=255)

                                                                                                                                cGAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAatgatg                  >  1:4700861/1‑150 (MQ=255)

                                                                                                                                 gAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGAtgt                 >  2:4137427/1‑150 (MQ=255)

                                                                                                                                 gAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGAtgt                 >  1:1932208/1‑150 (MQ=255)

                                                                                                                                 gAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGAtgt                 <  2:1164878/150‑1 (MQ=255)

                                                                                                                                 gAATATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGAtgt                 <  1:2408695/150‑1 (MQ=255)

                                                                                                                                  aaTATTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGAtgtg                >  1:3968215/1‑150 (MQ=255)

                                                                                                                                  aaTATTGTTTTCACCATGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGAtgtg                <  2:4310170/150‑1 (MQ=255)

                                                                                                                                    tatTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTa              <  1:4268735/150‑1 (MQ=255)

                                                                                                                                     atTGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTAc             >  1:3728110/1‑150 (MQ=255)

                                                                                                                                      ttGTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACa            >  1:2700720/1‑150 (MQ=255)

                                                                                                                                        gTTTTCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCTCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACAtt          <  1:955382/150‑1 (MQ=255)

                                                                                                                                         ttttCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTg         <  2:1535043/150‑1 (MQ=255)

                                                                                                                                         ttttCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTg         <  2:243825/150‑1 (MQ=255)

                                                                                                                                          tttCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGt        <  2:676635/150‑1 (MQ=255)

                                                                                                                                           ttCACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTc       >  1:1869516/1‑150 (MQ=255)

                                                                                                                                             cACCTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTcgc     <  1:3128202/150‑1 (MQ=255)

                                                                                                                                                cTTGAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTcgccgc  <  2:2757221/150‑1 (MQ=255)

AGGCGATGGCTCGGGCGGTCGTAAAGCCTGCATCCCGTGCTCGTCGGCGTCTTTCGTTTGATTAAAGGTAAAGCTATCAGCACTCAACAAACTGATGCTGTTCATTAAGACGATAGCGGACTCCGGCTCGAATATTGTTTTCACCTTCAATGGCGACGGCGAAGCGAACTCTTTCGCCGTCATGATATGCCAGTGCGGCGCTTCCTCCTGGCCCGAGAATAATTGAGTCGACCACGCCCGTGCTCACTACCGTTGAATTTTCACCGCTGGCAATGATGTGTACATTGTCGCCGC  >  AP012306/1430254‑1430547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`