Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,226,5570AG25.0% 428.5 / 70.1 188G55G (GGA→GGG) ydcUpredicted spermidine/putrescine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (20/121);  new base G (47/0);  total (67/121)
Fisher's exact test for biased strand distribution p-value = 1.06e-28
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 214 total aligned reads displayed.

TGCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGC  >  AP012306/1226409‑1226706
                                                                                                                                                    |                                                                                                                                                      
  cAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAttt                                                                                                                                                     <  1:237045/150‑1 (MQ=255)
  cAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGCGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGttt                                                                                                                                                     >  1:397410/1‑150 (MQ=255)
   aaTCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAtttt                                                                                                                                                    <  1:3654062/150‑1 (MQ=255)
       aCCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATAc                                                                                                                                                >  1:1212772/1‑150 (MQ=255)
       aCCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATAc                                                                                                                                                >  1:2869637/1‑150 (MQ=255)
          ttCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACttt                                                                                                                                             <  1:2194170/150‑1 (MQ=255)
           tCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTTTTCtttt                                                                                                                                            >  2:1072772/1‑150 (MQ=255)
              cGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGAc                                                                                                                                         <  1:2806883/150‑1 (MQ=255)
               gTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTTTAAttttttag                                                                                                                                        >  1:923513/1‑146 (MQ=255)
                tCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTCttttttcgg                                                                                                                                       >  2:2760572/1‑145 (MQ=255)
                 ccAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGAt                                                                                                                                      <  2:113279/150‑1 (MQ=255)
                  cAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGAtt                                                                                                                                     <  1:2030910/150‑1 (MQ=255)
                  cAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGAtt                                                                                                                                     <  1:2885596/150‑1 (MQ=255)
                   aGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGAttt                                                                                                                                    <  1:47917/150‑1 (MQ=255)
                     gTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCa                                                                                                                                  <  1:3728716/150‑1 (MQ=255)
                      tCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCAc                                                                                                                                 <  1:3493950/150‑1 (MQ=255)
                       cTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTCAcc                                                                                                                                >  1:3356146/1‑150 (MQ=255)
                       cTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCAcc                                                                                                                                <  1:2262304/150‑1 (MQ=255)
                       cTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCAcc                                                                                                                                <  1:3368755/150‑1 (MQ=255)
                        tGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTATTTTTTCGAttttccca                                                                                                                               >  2:1629524/1‑145 (MQ=255)
                         gggTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCAt                                                                                                                              <  2:2021047/150‑1 (MQ=255)
                           gTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGt                                                                                                                            <  1:3853896/150‑1 (MQ=255)
                            tAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTATTTTTTCGATTTTACCATGTc                                                                                                                           >  2:3478837/1‑150 (MQ=255)
                             aaGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCg                                                                                                                          <  2:545704/150‑1 (MQ=255)
                              aGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCgg                                                                                                                         <  1:2953945/150‑1 (MQ=255)
                               ggTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTTACCATGTCGGt                                                                                                                        >  1:3159613/1‑150 (MQ=255)
                                  gtCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTGACGATTTTACCCTGTCGGTAAc                                                                                                                     >  1:2981361/1‑150 (MQ=255)
                                  gtCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAAc                                                                                                                     <  2:4259455/150‑1 (MQ=255)
                                     cGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATACTTTTGACGATTTCACCATGTCGGTAACGcc                                                                                                                  >  1:112566/1‑150 (MQ=255)
                                      ggTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCg                                                                                                                 <  2:283553/150‑1 (MQ=255)
                                         ttcttcTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGaa                                                                                                              <  1:4341143/150‑1 (MQ=255)
                                           cttctGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTTCGATTTTACCATTTCGGTAACCCCGGAACt                                                                                                            >  2:1538341/1‑150 (MQ=255)
                                               tGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACg                                                                                                        <  1:3799845/150‑1 (MQ=255)
                                                ggCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATCTCGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTTTTCTTTTTTCGATTTTACCATGTTGGTGAAGCCGGGACTGTCGc                                                                                                       >  2:2490591/1‑150 (MQ=255)
                                                 gCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCt                                                                                                      <  2:369229/150‑1 (MQ=255)
                                                    tAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGc                                                                                                   <  1:2252976/150‑1 (MQ=255)
                                                    tAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGc                                                                                                   <  1:728505/150‑1 (MQ=255)
                                                    tAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGc                                                                                                   <  1:1794979/150‑1 (MQ=255)
                                                            ggctggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATcc                                                                                           <  1:183650/150‑1 (MQ=255)
                                                              ctggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATACTTTTGACGATTTTACCATGTCGGTAACGCCCGGACTGACGCTCGCGAAAATccct                                                                                         >  1:223597/1‑148 (MQ=255)
                                                               tggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTg                                                                                        <  2:1449407/150‑1 (MQ=255)
                                                                ggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTTCGATTTTACCATGTCGGTAACGCCGGGACTGACGCTGGCGGATATCCGTgc                                                                                       >  2:3810122/1‑150 (MQ=255)
                                                                ggggctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTgc                                                                                       <  2:3159656/150‑1 (MQ=255)
                                                                   gctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCt                                                                                    <  1:2496010/150‑1 (MQ=255)
                                                                    ctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑g                                                                                  <  1:2034567/150‑1 (MQ=255)
                                                                    ctgTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑g                                                                                  <  2:2068376/150‑1 (MQ=255)
                                                                      gTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATTCTTTTGACGGTTTTCCCATGTCGGTAACGCCGGGAATGACGCCGGCGGAAATTCGTGCGCT‑Gtt                                                                                >  2:2593009/1‑150 (MQ=255)
                                                                       tttttACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑Gttt                                                                               <  1:3456773/150‑1 (MQ=255)
                                                                        ttttACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTa                                                                              <  2:3956761/150‑1 (MQ=255)
                                                                             ctgctgCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCg                                                                         <  1:4101187/150‑1 (MQ=255)
                                                                             ctgctgCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCg                                                                         <  2:328777/150‑1 (MQ=255)
                                                                             ctgctgCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCg                                                                         <  2:4325296/150‑1 (MQ=255)
                                                                             ctgctgCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCg                                                                         <  1:3702125/150‑1 (MQ=255)
                                                                               gctgctTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGc                                                                       <  2:2391086/150‑1 (MQ=255)
                                                                               gctgctTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGc                                                                       <  2:2313271/150‑1 (MQ=255)
                                                                                   ctTGGCTCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAAt                                                                   <  2:933257/150‑1 (MQ=255)
                                                                                   ctTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTATTTTTTCGATTTTACCATGTCGGGAAAGCCCGGAATGACGCTGGGGAATATCCGTGCGCT‑GTTTTATTCCGCGGAt                                                                   >  2:2892621/1‑150 (MQ=255)
                                                                                   ctTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTTCGATTTTTCCATGTCGGTAAAGCCGGGAACGACGCCGGCGGAAATTCGTGCGCT‑GTTTTATTCCGCGAAt                                                                   >  2:3177457/1‑150 (MQ=255)
                                                                                   ctTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAAt                                                                   <  2:2323929/150‑1 (MQ=255)
                                                                                    ttGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAAtt                                                                  <  1:1968187/150‑1 (MQ=255)
                                                                                     tGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTa                                                                 <  1:1025650/150‑1 (MQ=255)
                                                                                        cccGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGa                                                              <  2:2434222/150‑1 (MQ=255)
                                                                                         ccGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTGCGATTTCACCCTGTCGGTAACGCCGGAACTGACGCCGGCGGATATCCGTGCGCT‑GGTTTATCCGGCGAATTACGAc                                                             >  2:3377435/1‑150 (MQ=255)
                                                                                          cGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTCTCCCTGTCGGGAACGCCCGGACTGACGCTGGCGGATATCCGTGCGGT‑GTTTTATACGGCGAATTTCGGca                                                            >  2:1971541/1‑150 (MQ=255)
                                                                                           gCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTATTTTTACGCTTTTACCACGTCGGTGATGCCCGGACTGACGCCGGCGGATAATCGCGGGCTGGTTTATTCCGG‑GAAtttacacaa                                                           >  2:4398134/1‑143 (MQ=255)
                                                                                           gCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTGCGATTTTACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTTATCCGGCGAATTACGAcat                                                           >  2:463574/1‑150 (MQ=255)
                                                                                            cTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTCTTTTTTCGATTTTCCCATGTCGGTAACGCCGGGACTGACGCTGGCGAATATCCGTGGGCT‑GTTTAATCCGGCGGATTACGGcatc                                                          >  2:3873195/1‑150 (MQ=255)
                                                                                             tAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGAcatca                                                         <  2:1951548/150‑1 (MQ=255)
                                                                                              aaTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTTTACTTTTTGCGATTTTACCATGTCGGGAACGCCCGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATTCGGCGGAATACGAcctcat                                                        >  2:1304524/1‑150 (MQ=255)
                                                                                              aaTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGAcatcat                                                        <  2:4210833/150‑1 (MQ=255)
                                                                                              aaTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGAcatcat                                                        <  1:74707/150‑1 (MQ=255)
                                                                                               aTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATACTTTTTTCGATTTTCCCCTATCGGGAAAGCCCGGAATGACGCTGGCGAATATCCGTGCGCT‑GTTTTATCCCGCGGATTTCGACATCAtt                                                       >  2:4134378/1‑150 (MQ=255)
                                                                                               aTGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGACATCAtt                                                       <  2:9575/150‑1 (MQ=255)
                                                                                                    gTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGACATCATTCTCCg                                                  <  1:457451/150‑1 (MQ=255)
                                                                                                      ttGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGACATCATTCTCCGCa                                                <  2:869556/150‑1 (MQ=255)
                                                                                                       tGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATACTTTTTGCGATTTCACCATGTCGGTAACGCCGGGACTGACGCCGGGGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGACATCATTCTCCCCAc                                               >  2:737840/1‑150 (MQ=255)
                                                                                                        ggCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGACATCATTCTCCGCACg                                              <  1:1868868/150‑1 (MQ=255)
                                                                                                               tCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTa                                       <  1:1547426/150‑1 (MQ=255)
                                                                                                               tCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTa                                       <  1:2082841/150‑1 (MQ=255)
                                                                                                                      cGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGGTTTTACCATGTCGGTAAAGCCGGGACTGACGCTGGCGGATATTCGTGCGCT‑GTTTAATCCGGCGAATTACGACATCATTTTCCCCCCGCTGACTATGGCtgt                                >  2:3519223/1‑150 (MQ=255)
                                                                                                                       ggCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCtgtg                               <  1:1470876/150‑1 (MQ=255)
                                                                                                                       ggCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCtgtg                               <  1:163202/150‑1 (MQ=255)
                                                                                                                            gctgctGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTTACCATGTCGGGAAAGCCGGGACTGACGCTGGGGAATATCCGTGCGCT‑GTTTTATCCGGCGAATTACGACATCATTTTCCGCACGCTGACTATGGCTGGGGGAgg                          >  2:4108124/1‑149 (MQ=255)
                                                                                                                             ctgctgACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTc                         <  1:3335512/150‑1 (MQ=255)
                                                                                                                                  gACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTAt                    >  1:566601/1‑150 (MQ=255)
                                                                                                                                  gACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGACATCATTCTCCGCACCCTGACTATGGCTGTGGCAGTCACCAt                    >  1:1269898/1‑150 (MQ=255)
                                                                                                                                    cacTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCg                  <  1:391662/150‑1 (MQ=255)
                                                                                                                                     acTGTTATGGCAGGGATTTTATACTTTTTACGATTTTACCATGTCGGGAACGCCGGAACTGACGCTGGCGAAAATCCGTGCGCT‑GTTTTATCCGGCGAATTACGACCTCATTTTCCGCCCGCCGACTTTTGCTGTGGGCGGCACTAtttc                 >  2:4351479/1‑147 (MQ=255)
                                                                                                                                     acTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGc                 <  1:356669/150‑1 (MQ=255)
                                                                                                                                       tGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCa               <  1:2054634/150‑1 (MQ=255)
                                                                                                                                         ttATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAgc             <  2:2372353/150‑1 (MQ=255)
                                                                                                                                          tATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGGCGTCACTATCGCCcgcg            >  2:4315763/1‑150 (MQ=255)
                                                                                                                                            tGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGcc          >  2:159327/1‑150 (MQ=255)
                                                                                                                                             ggCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCa         >  2:1827300/1‑150 (MQ=255)
                                                                                                                                              gCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCAt        >  1:2439626/1‑150 (MQ=255)
                                                                                                                                              gCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCAt        >  2:1638462/1‑150 (MQ=255)
                                                                                                                                                aGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTc      >  2:3633724/1‑150 (MQ=255)
                                                                                                                                                  ggATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTg    >  1:3041480/1‑150 (MQ=255)
                                                                                                                                                  ggATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTg    <  1:2652491/150‑1 (MQ=255)
                                                                                                                                                    aTTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGAT‑GTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGc  <  1:882462/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
TGCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCCGGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCT‑GTTTAATCCGGCGAATTACGACATCATTCTCCGCACGCTGACTATGGCTGTGGCAGTCACTATCGCCAGCGCCATTCTGGC  >  AP012306/1226409‑1226706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑