Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,309,9140AC27.6% 227.8 / 10.1 116L23F (TTA→TTC) lsrCAI2 transporter
Reads supporting (aligned to +/- strand):  ref base A (51/33);  new base C (0/32);  total (51/65)
Fisher's exact test for biased strand distribution p-value = 9.90e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 186 total aligned reads displayed.

CTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAAT  >  AP012306/1309765‑1310063
                                                                                                                                                     |                                                                                                                                                       
ctctGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTa                                                                                                                                                         <  1:2281256/150‑1 (MQ=255)
 tctGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTAc                                                                                                                                                        >  1:4263859/1‑150 (MQ=255)
    gCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCg                                                                                                                                                     >  2:4056944/1‑150 (MQ=255)
    gCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCg                                                                                                                                                     <  2:1591778/150‑1 (MQ=255)
       cTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGGGCTGCTGGTTGTATTACCCgggt                                                                                                                                                  >  2:2980394/1‑148 (MQ=255)
            cGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTtctc                                                                                                                                             <  1:1836658/150‑1 (MQ=255)
                cgcgATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GAcc                                                                                                                                        >  1:3512783/1‑150 (MQ=255)
                 gcgATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGCTTTCTC‑GACCg                                                                                                                                       >  1:281513/1‑150 (MQ=255)
                 gcgATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGGGCTGCTGTTTGTATTACCCGGGTTTCTC‑GACCg                                                                                                                                       >  2:1597874/1‑150 (MQ=255)
                  cgATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGc                                                                                                                                      >  2:2297816/1‑150 (MQ=255)
                    atatTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCa                                                                                                                                    <  1:724359/150‑1 (MQ=255)
                    atatTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGGGCTGCTGTTTGTATTACCCCGGTTTCTC‑GACCGCCa                                                                                                                                    >  1:1017903/1‑150 (MQ=255)
                      atTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGGTTTCTC‑GACCGCCAGt                                                                                                                                  >  2:22778/1‑150 (MQ=255)
                           tGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGGGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCCCTGCTGGCGGTGGTGCTGCTGTTTGTATTCCCCGGTTTTCTC‑GACCGCCAGTATTTa                                                                                                                             <  2:3848584/150‑1 (MQ=255)
                           ggTCGAGATTATTAGGCGCTTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTAATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGGGGTGGTGCTGCTGTTTTTTTTCCCCGTTTTTCTC‑GACCGCCAGTATTTa                                                                                                                             <  2:549006/149‑1 (MQ=255)
                            gTCGAGACTATTATGCGCGTTGCTTTCGGCGATAGTCAGCGTCGGGGGGCGTCATGCTGAGTTTTATTCAGAACACCCGTGAAACCCCGGCCCGGCGGGCGGGGGTGCTGCTTTTTTTATTCCCCGTTTTTCTC‑GACCGCCAGTATTTaa                                                                                                                            <  2:3928296/150‑1 (MQ=255)
                            gTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTaa                                                                                                                            >  1:1934030/1‑150 (MQ=255)
                             tCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTCCCCGGTTTTCTC‑GACCGCCAGTATTTAAg                                                                                                                           <  1:1543065/150‑1 (MQ=255)
                              cGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAgt                                                                                                                          >  1:3294741/1‑150 (MQ=255)
                               gagaCTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAgtg                                                                                                                         >  1:435704/1‑150 (MQ=255)
                                agaCTTTTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCTCTGCTGGGGGTGGTGCTGCTGTTTGTATTCCCCGGTTTTCTC‑GACCGCCAGTATTTAAgtgt                                                                                                                        <  2:3997950/150‑1 (MQ=255)
                                     attatGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGGTGCTGTTTGTATTACCCGGGTTTCTC‑GACCGCCAGTATTTAAGTGTGCaaa                                                                                                                   >  2:472678/1‑150 (MQ=255)
                                     attatGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTAACCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGTaaa                                                                                                                   >  2:2942069/1‑150 (MQ=255)
                                      ttatGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGGGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAAc                                                                                                                  >  2:541212/1‑150 (MQ=255)
                                       tatGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTTTTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACg                                                                                                                 <  1:3099137/150‑1 (MQ=255)
                                          gcgcGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTg                                                                                                              >  1:1232091/1‑150 (MQ=255)
                                            gcgTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTTTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGAc                                                                                                            >  2:1949748/1‑150 (MQ=255)
                                            gcgTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGAc                                                                                                            >  2:540648/1‑150 (MQ=255)
                                             cgTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAATTTTATTCAGAACAACCGTGAAATCCCGGCACTGCGGGGGGTGGTGCTGTTGTTTTTATTCCCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGAcc                                                                                                           <  2:2351065/150‑1 (MQ=255)
                                             cgTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGAcc                                                                                                           >  1:290918/1‑150 (MQ=255)
                                             cgTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGAcc                                                                                                           <  1:319715/150‑1 (MQ=255)
                                              gTTGCCTTCGGCGATAGTCAGCGTCAGGGGGGGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTCCCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCa                                                                                                          <  1:1318634/150‑1 (MQ=255)
                                              gTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCa                                                                                                          >  2:1731806/1‑150 (MQ=255)
                                              gTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGGGCTGCTGTTTGTATTATCCGGGTTTCTC‑GACCGCCCGTATTTTAGTGTGCAAACGCTGGCCa                                                                                                          >  2:4256033/1‑150 (MQ=255)
                                                   cTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGtt                                                                                                     <  1:3350988/150‑1 (MQ=255)
                                                   cTTCGCCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGGTGCTGTTTGTATTAACCGGTTTTCTC‑GACCGCCAGTATTTTAGTGTGCGAACGCTGACCATgggt                                                                                                     >  2:3031863/1‑148 (MQ=255)
                                                       ggCGATAGTCAGCGTCAGGAGGCGCCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGGGCTTGTGTTTGTATTTCCCGGCTTTTCC‑CACCGCCAGGATTTTAGGGGGCAAACGCCCACCATGGGTtat‑a                                                                                                >  2:1643719/1‑150 (MQ=255)
                                                        gCGATAGTCAGCGTCAGGGGGCTTCATGCTGAAGTTTATTCAGAACAACCGGAAAATCCCGGCACTGCGGGCGGTGGTGCTGCTGTTTGTATTCCCCGTTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑ag                                                                                               <  2:633436/150‑1 (MQ=255)
                                                              gTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTAAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTCCCCGTTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAgcgc                                                                                         <  1:1987226/150‑1 (MQ=255)
                                                                     tCAGGGGGCGTCATGCTGAAGTTTATTCAGAACACCCGTGAAATCCCGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTCCCCGTTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAatc                                                                                  <  2:959512/150‑1 (MQ=255)
                                                                      cAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAatcc                                                                                 <  1:3467070/150‑1 (MQ=255)
                                                                          aggCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGGGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAatcctgat                                                                             >  2:3514350/1‑150 (MQ=255)
                                                                                 aTGCTGAATTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTCCCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCctgctg                                                                      <  2:2908104/150‑1 (MQ=255)
                                                                                   ggtGAATTTTATGCAGAACACCCGTAAAATCACGGCACTGCGGGCGGGGGTGGTGCTTTTTGTATTACCGGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGc                                                                    <  2:727182/148‑1 (MQ=255)
                                                                                   gCTGAAGTTTATTCAGAACACCCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCGGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGc                                                                    <  1:368417/150‑1 (MQ=255)
                                                                                     tGAATTTTATTCAGAACACCCGTAAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTCCCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCaa                                                                  <  1:707386/150‑1 (MQ=255)
                                                                                      gAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAAt                                                                 >  1:3530689/1‑150 (MQ=255)
                                                                                      gAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGGGGGGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAAt                                                                 >  2:285345/1‑150 (MQ=255)
                                                                                       aattttATTCAAAACAACCGTGAAATCACGGCACTGGTGGCGGTGGTGCTGCTTTTTGTATTACCCGTTTTCCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATg                                                                <  2:3114427/147‑1 (MQ=255)
                                                                                       aaGTTTATTCAGAACACCCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTCCCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATg                                                                <  1:3145276/150‑1 (MQ=255)
                                                                                       aaGTTTATTCAGAACAACCGGAAAACCCCGGCCCTGCTGGCGGTGGTGCTGCTGTTTTTATCCCCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATg                                                                <  2:1606814/150‑1 (MQ=255)
                                                                                        attttATTCAGAACAACCGGGAAATCACGGCCCTGCTGGGGGTGGTGCTGCTGTTTGTATTCCCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATgg                                                               <  2:1686096/148‑1 (MQ=255)
                                                                                            tATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGgcgc                                                           >  1:267695/1‑150 (MQ=255)
                                                                                            tATTCAGAACAACCGTAAAATCACGGCACTGCGGGCGGTGGTGCTGCTTTTTGTATTCCCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGgcgc                                                           <  1:2302098/150‑1 (MQ=255)
                                                                                               tCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTTTTTGTATTCCCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGAc                                                        <  2:4059557/150‑1 (MQ=255)
                                                                                                cAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGGTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACg                                                       >  1:992928/1‑150 (MQ=255)
                                                                                                      aacCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTa                                                 >  2:3712015/1‑150 (MQ=255)
                                                                                                      aacCGCAAAATCCCGCAACGGCTGGGTGTGGTGCTGCTGTTTGATTTACCCGTTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTa                                                 <  2:2216327/150‑1 (MQ=255)
                                                                                                        ccGTGAAATCACGGCACTGCTGGCGGTGGTGATGCTGTTTTTATTACCCCGTTTTCTC‑GACCACCAGTATTTAAGTGTGCAAACGCTGACCAAGGGTTATAAGCA‑CGCGCAAATCCTGATCCTGCTGGCGATTGGGGCGACGCTGGTAAt                                               >  2:4170514/1‑150 (MQ=255)
                                                                                                        ccGTAAAACCACGGCCCGGCTGGGGGGGTTGGGGCTGTTTGTATTACCTGTTTTTCCC‑CACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAAt                                               <  2:174026/150‑1 (MQ=255)
                                                                                                         cGTGAAATCCCGGCTCTGCGGGCGGTGGTGCTGCTGTTTGTATTCCCCGTTTTTCCC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATg                                              <  2:3510970/150‑1 (MQ=255)
                                                                                                         cGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTCCCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATg                                              <  1:2465785/150‑1 (MQ=255)
                                                                                                         cGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATg                                              <  2:2675086/150‑1 (MQ=255)
                                                                                                         cGTGAAACCACGGCCCTGCTGGCGGTGGTGCTGCTGTTTGTATTCCCCGGTTTTCCC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATg                                              <  1:1362620/150‑1 (MQ=255)
                                                                                                            gAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCtt                                           >  1:4062788/1‑150 (MQ=255)
                                                                                                                tCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GATCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTAcgc                                       <  2:931863/150‑1 (MQ=255)
                                                                                                                    ggCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCaa                                   <  1:2368303/150‑1 (MQ=255)
                                                                                                                    ggCACTGCTGGCGGTGGGGCTGCTGTTTGTATTACCCGGTTTTCTC‑GGCCGCCAGGATTTAAGGGTGCAAACGCTGACCCTGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGCCGCTGGTAATGCTTTCGCGCaa                                   >  2:2802193/1‑150 (MQ=255)
                                                                                                                     gCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGGTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAat                                  >  1:53588/1‑150 (MQ=255)
                                                                                                                      cgcggcgggCGGTGGTGCTGCTGTTTGTATTCCCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAata                                 <  1:981924/143‑1 (MQ=255)
                                                                                                                      ccctgctgGCGGTGGTGCTGCTGTTTGTATTCCCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAata                                 <  2:683326/148‑1 (MQ=255)
                                                                                                                      cACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAata                                 >  1:3564123/1‑150 (MQ=255)
                                                                                                                      cACTGCTGGCGGGGGTGCTGCTGTTTTTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCCTGGTTTAT‑AGCGGCGCGGAAATCCTGATCCTGCTGGCAATGGGCGCGGCGGTGGGAATGCTTACGCGCAata                                 >  2:1334999/1‑150 (MQ=255)
                                                                                                                       aCTGCTGGCGGTGGTGTTGCTGTTTGTATTCCCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAatat                                <  1:2076869/150‑1 (MQ=255)
                                                                                                                        ctgctgGCGGTGGTGCTGCTGTTTGTATTCCCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATAtt                               <  1:3955556/150‑1 (MQ=255)
                                                                                                                        ctgctgGCGGTGGGGCTGCTGTTTGTATTACCCGGGTTTCTC‑GACCGCCCGGATTTAAGGGTGCAAACGCTGACCATGGGTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGGGCGGCGCTGGGAATGCTTTCGCGCAATAtt                               >  2:2184982/1‑150 (MQ=255)
                                                                                                                          gctgGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGa                             <  1:2733261/150‑1 (MQ=255)
                                                                                                                          gctgGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGa                             <  1:2543939/150‑1 (MQ=255)
                                                                                                                          ctggggggtggcGCCGCT‑TTTGTTTTCCCCCTTTTTCCCGGACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGa                             <  2:1060507/144‑1 (MQ=255)
                                                                                                                             ggCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGt                          >  2:3404543/1‑150 (MQ=255)
                                                                                                                                ggtggtGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTc                       <  2:1256505/150‑1 (MQ=255)
                                                                                                                                  tggtgCTGCTGTTTGTTTTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAg                     <  2:2536674/150‑1 (MQ=255)
                                                                                                                                  tggtgCTGCTGTTTGTATTCCCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAg                     <  2:4062788/150‑1 (MQ=255)
                                                                                                                                     tgcgcctgTTTGTATTCCCCGTTTTCCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTgg                  <  2:1868665/145‑1 (MQ=255)
                                                                                                                                      gctggtTTTTGTATTCCCCGGTTTTCTG‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTggg                 <  2:2352047/150‑1 (MQ=255)
                                                                                                                                      gctgctGTTTGTATTACCCGTTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTggg                 <  1:975633/150‑1 (MQ=255)
                                                                                                                                       ctgctgTTTGTATTCCCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGt                <  1:275743/150‑1 (MQ=255)
                                                                                                                                       ctgctgTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGt                <  1:1674127/150‑1 (MQ=255)
                                                                                                                                       ctgctgTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGt                <  1:3622569/150‑1 (MQ=255)
                                                                                                                                         gctgTTTGTATTACCGGTTTTTCAC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTc              <  1:687696/150‑1 (MQ=255)
                                                                                                                                         gctgTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTc              <  1:2311553/150‑1 (MQ=255)
                                                                                                                                              ttGTATTCCCGGTTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTa         <  1:1021931/150‑1 (MQ=255)
                                                                                                                                              ttGTATTCCCCGTTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTa         <  2:1799575/150‑1 (MQ=255)
                                                                                                                                               tGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTAc        <  1:3481768/150‑1 (MQ=255)
                                                                                                                                                 tATTCCCCGTTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCg      <  2:2097205/150‑1 (MQ=255)
                                                                                                                                                 tATTCCCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCg      <  2:2182603/150‑1 (MQ=255)
                                                                                                                                                 tATTACCCGGTTTTCTC‑GACAGCCAGTATTTAAGTGTGCAAACGCTGACAATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTAcag      >  2:4248066/1‑148 (MQ=255)
                                                                                                                                                  aTTCCCCGTTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCgg     <  2:3830224/150‑1 (MQ=255)
                                                                                                                                                     aCCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAAt  >  2:2596582/1‑150 (MQ=255)
                                                                                                                                                     aCCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAAt  >  2:330799/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
CTCTGCACTGACCGAGCGCGATATTAATGTCGAGACTATTATGCGCGTTGCCTTCGGCGATAGTCAGCGTCAGGAGGCGTCATGCTGAAGTTTATTCAGAACAACCGTGAAATCACGGCACTGCTGGCGGTGGTGCTGCTGTTTGTATTACCCGGTTTTCTC‑GACCGCCAGTATTTAAGTGTGCAAACGCTGACCATGGTTTAT‑AGCAGCGCGCAAATCCTGATCCTGCTGGCAATGGGCGCGACGCTGGTAATGCTTACGCGCAATATTGATGTTTCAGTGGGTTCGATTACCGGAAT  >  AP012306/1309765‑1310063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑