BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	1,437,493	A→T	E71D (GAA→GAT)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	1,437,493	0	A	T	100.0%	1009.7 / NA	243	E71D (GAA→GAT)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (149/94); total (149/94)

Only 100 of 247 total aligned reads displayed.

AATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGG  >  AP012306/1437346‑1437639

aaTGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGatat                                                                                                                                                  >  1:962185/1‑150 (MQ=255)

   gCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCg                                                                                                                                               >  1:3499070/1‑150 (MQ=255)

    cTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGc                                                                                                                                              >  1:863076/1‑150 (MQ=255)

      ggACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCAc                                                                                                                                            >  1:518853/1‑150 (MQ=255)

      ggACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCAc                                                                                                                                            >  1:3472274/1‑150 (MQ=255)

       gACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCAcc                                                                                                                                           >  2:3891534/1‑150 (MQ=255)

        aCGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCa                                                                                                                                          >  1:2239419/1‑150 (MQ=255)

        aCGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCa                                                                                                                                          >  1:1307586/1‑150 (MQ=255)

         cGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCAt                                                                                                                                         >  2:3033548/1‑150 (MQ=255)

             ggCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGaaa                                                                                                                                     >  2:1774493/1‑150 (MQ=255)

               cATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACt                                                                                                                                   >  2:3998731/1‑150 (MQ=255)

                aTGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTg                                                                                                                                  >  1:1003449/1‑150 (MQ=255)

                 tGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTgg                                                                                                                                 >  1:1233631/1‑150 (MQ=255)

                     cGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAgg                                                                                                                             >  1:3748614/1‑150 (MQ=255)

                      gTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGc                                                                                                                            >  2:960963/1‑150 (MQ=255)

                       ttATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCg                                                                                                                           >  1:207404/1‑150 (MQ=255)

                                tGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAacgacg                                                                                                                  >  2:3394408/1‑150 (MQ=255)

                                 gAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGt                                                                                                                 >  1:1420555/1‑150 (MQ=255)

                                 gAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGt                                                                                                                 >  2:4374420/1‑150 (MQ=255)

                                     ttCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTtct                                                                                                             >  2:3876574/1‑150 (MQ=255)

                                          tcATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGaa                                                                                                        >  1:1499269/1‑150 (MQ=255)

                                            aTGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAg                                                                                                      >  1:4109668/1‑150 (MQ=255)

                                                tGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTgg                                                                                                  >  1:2776418/1‑150 (MQ=255)

                                                     aggaaaaaCCCGGTCAGCGAATCAAAACTCTGCGAAGGGTGAGAAAAAAAACGGAAAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTAGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGa                                                                                             <  1:3074370/144‑1 (MQ=255)

                                                     aggCAGACCACGGTCACCGCATACAGAATCGGCACAACGAGATAAGAAAAAATGAAAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGa                                                                                             <  2:4240048/148‑1 (MQ=255)

                                                         aGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCtt                                                                                         >  2:1483278/1‑150 (MQ=255)

                                                          gAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCttt                                                                                        >  1:2999642/1‑150 (MQ=255)

                                                           aaCACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTa                                                                                       >  2:1155430/1‑150 (MQ=255)

                                                           aaCACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTa                                                                                       >  1:3817325/1‑150 (MQ=255)

                                                            acccGGTCAGCACATTAAAAATCGGCGAAGCGAGAAGAAAAAAACTGAAAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTAc                                                                                      <  1:3406638/147‑1 (MQ=255)

                                                             cacGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACt                                                                                     >  1:3109990/1‑150 (MQ=255)

                                                             cacGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACt                                                                                     >  1:1935522/1‑150 (MQ=255)

                                                             cacGCTCAGCGAATTCAGAACCGGCGAAACGGGAAGAGCAAAAC‑GGTAAAAGCCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACt                                                                                     <  1:1827214/150‑1 (MQ=255)

                                                              acGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACtt                                                                                    >  2:2841103/1‑150 (MQ=255)

                                                                     gcgcATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTcaccac                                                                             >  2:1211924/1‑150 (MQ=255)

                                                                     gcgcATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTcaccac                                                                             >  1:2655734/1‑150 (MQ=255)

                                                                       gcATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTg                                                                           >  2:3643117/1‑150 (MQ=255)

                                                                       ctaTTAAGATTCTGCGCAAGGTGAGAAAAAAAATTGAAAAAC‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTg                                                                           <  2:656945/148‑1 (MQ=255)

                                                                         ataaaGAATCTGCGAAACGTGATAAAAAAAACGGAAAAAC‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGAt                                                                         <  2:4162692/146‑1 (MQ=255)

                                                                          ttCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATa                                                                        >  2:2213582/1‑150 (MQ=255)

                                                                             aGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAAt                                                                     >  1:1147071/1‑150 (MQ=255)

                                                                             aGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAAt                                                                     >  1:3965356/1‑150 (MQ=255)

                                                                              gAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATc                                                                    >  1:759746/1‑150 (MQ=255)

                                                                                aTCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTg                                                                  >  1:4203447/1‑150 (MQ=255)

                                                                                aTCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTg                                                                  >  1:39873/1‑150 (MQ=255)

                                                                                 tCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGt                                                                 >  1:1557720/1‑150 (MQ=255)

                                                                                 tCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGt                                                                 >  1:1654917/1‑150 (MQ=255)

                                                                                   ggcgaAACGTGATGAG‑AAAAATGGAAAAACCCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTa                                                               <  1:325333/149‑1 (MQ=255)

                                                                                    gcgcAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTAt                                                              >  2:782809/1‑150 (MQ=255)

                                                                                     cgaaaCGTGAGAAGAAAAACTGAAAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATc                                                             <  2:2155862/147‑1 (MQ=255)

                                                                                           gTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAAc                                                       >  2:526131/1‑150 (MQ=255)

                                                                                           gTGATGAAAAAAACTGAAAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAAc                                                       <  1:701153/150‑1 (MQ=255)

                                                                                            tgatgaGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACa                                                      >  2:1958454/1‑150 (MQ=255)

                                                                                            tgatgaGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACa                                                      >  1:2900647/1‑150 (MQ=255)

                                                                                            tgatgaGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACa                                                      >  2:3589571/1‑150 (MQ=255)

                                                                                             gagaaaaaaaaATGGAAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAg                                                     <  2:2450964/143‑1 (MQ=255)

                                                                                                 aaaaaaaATGGAAAAACCCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGa                                                  <  2:1615423/147‑1 (MQ=255)

                                                                                                       actgaaaaaa‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGtt                                           <  1:227236/144‑1 (MQ=255)

                                                                                                        gtGGAAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTg                                          <  1:2404428/149‑1 (MQ=255)

                                                                                                        ataatAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTg                                          <  2:3654836/146‑1 (MQ=255)

                                                                                                            tAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGt                                      >  1:2025820/1‑150 (MQ=255)

                                                                                                             aaaa‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTa                                     >  1:459424/1‑150 (MQ=255)

                                                                                                               aa‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAAc                                   >  2:3518592/1‑150 (MQ=255)

                                                                                                                a‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACg                                  <  1:4310358/150‑1 (MQ=255)

                                                                                                                  ccgccgCTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGt                                 <  2:2069299/150‑1 (MQ=255)

                                                                                                                     ccgcTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATg                              <  2:3348947/150‑1 (MQ=255)

                                                                                                                      cgcTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGa                             <  1:365747/150‑1 (MQ=255)

                                                                                                                       gcTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGaa                            >  1:4023051/1‑150 (MQ=255)

                                                                                                                       gcTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGaa                            >  2:3258044/1‑150 (MQ=255)

                                                                                                                        cTATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAg                           <  1:1688500/150‑1 (MQ=255)

                                                                                                                         tATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAgg                          <  1:117374/150‑1 (MQ=255)

                                                                                                                         tATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAgg                          <  1:99505/150‑1 (MQ=255)

                                                                                                                         tATCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAgg                          >  1:3554449/1‑150 (MQ=255)

                                                                                                                          aTCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGt                         >  2:2761765/1‑150 (MQ=255)

                                                                                                                          aTCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGt                         <  1:2865104/150‑1 (MQ=255)

                                                                                                                          aTCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGt                         <  1:2738840/150‑1 (MQ=255)

                                                                                                                          aTCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGt                         <  2:474535/150‑1 (MQ=255)

                                                                                                                          aTCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGt                         <  2:1043988/150‑1 (MQ=255)

                                                                                                                           tCCTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGtt                        >  2:2559948/1‑150 (MQ=255)

                                                                                                                             cTGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTc                      <  2:2514035/150‑1 (MQ=255)

                                                                                                                              tGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCa                     <  2:2960942/150‑1 (MQ=255)

                                                                                                                              tGCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCa                     >  2:3022529/1‑150 (MQ=255)

                                                                                                                               gCTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCac                    >  1:2703271/1‑150 (MQ=255)

                                                                                                                                cTTGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCact                   <  2:1830859/150‑1 (MQ=255)

                                                                                                                                 ttGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCacta                  <  2:4355765/150‑1 (MQ=255)

                                                                                                                                  tGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCactac                 <  2:2915630/150‑1 (MQ=255)

                                                                                                                                  tGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCactac                 >  2:3100386/1‑150 (MQ=255)

                                                                                                                                  tGATACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCactac                 >  1:1584172/1‑150 (MQ=255)

                                                                                                                                     tACCAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGa              <  2:2381603/150‑1 (MQ=255)

                                                                                                                                        cAAAGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACct           <  2:1917091/150‑1 (MQ=255)

                                                                                                                                           aGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACctgtc        >  1:3877842/1‑150 (MQ=255)

                                                                                                                                           aGGTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACctgtc        >  2:1560370/1‑150 (MQ=255)

                                                                                                                                            ggTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACctgtct       >  2:1902598/1‑150 (MQ=255)

                                                                                                                                             gTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACctgtctg      >  1:1861/1‑150 (MQ=255)

                                                                                                                                             gTCCGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACctgtctg      <  2:129641/150‑1 (MQ=255)

                                                                                                                                               ccGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGtt    >  2:2717889/1‑150 (MQ=255)

                                                                                                                                                cGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTg   >  2:1102209/1‑150 (MQ=255)

                                                                                                                                                cGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTg   <  2:1663175/150‑1 (MQ=255)

                                                                                                                                                cGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTg   >  2:1204260/1‑150 (MQ=255)

                                                                                                                                                cGGATATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTg   >  1:830583/1‑150 (MQ=255)

AATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAA‑CCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGACGTTTCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGG  >  AP012306/1437346‑1437639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`