BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	1,587,616	A→C	L41R (CTG→CGG)	yobF ←	predicted protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	1,587,616	0	A	C	100.0%	994.7 / NA	243	L41R (CTG→CGG)	yobF	predicted protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (166/77); total (166/77)

Only 100 of 252 total aligned reads displayed.

CCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACC  >  AP012306/1587469‑1587762

 ccTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGc                                                                                                                                                 >  2:4199122/1‑150 (MQ=255)

  cTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCa                                                                                                                                                >  1:3749442/1‑150 (MQ=255)

     gATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATaa                                                                                                                                             >  1:2232668/1‑150 (MQ=255)

      aTAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAAc                                                                                                                                            >  1:3920934/1‑150 (MQ=255)

        aGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACAt                                                                                                                                          >  1:1390757/1‑150 (MQ=255)

         gCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATa                                                                                                                                         >  1:1522920/1‑150 (MQ=255)

          cGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAg                                                                                                                                        >  1:1122455/1‑150 (MQ=255)

          cGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAg                                                                                                                                        >  1:2436229/1‑150 (MQ=255)

           ggAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGa                                                                                                                                       >  2:1524651/1‑150 (MQ=255)

             agaAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATa                                                                                                                                     >  1:640054/1‑150 (MQ=255)

             agaAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATa                                                                                                                                     >  1:227660/1‑150 (MQ=255)

                aGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATaaaa                                                                                                                                  >  2:2386676/1‑150 (MQ=255)

                  tgtACAAACACCTTTTGGCTCCCATCACCGGGAGTAA‑GAAACCAAAACCTT‑TTATCCTCTTCGACCCACTTAA‑CCTGTCCTTTAATTTTCTCCATTGAAAAAATTCC‑TTAGTTTTTTTTCTCCGCCCGCCGGCATAACATAGATAAAACt                                                                                                                                <  2:743652/150‑1 (MQ=255)

                   gtACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTg                                                                                                                               >  2:759052/1‑150 (MQ=255)

                   gtACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTg                                                                                                                               >  1:3679543/1‑150 (MQ=255)

                   gtACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTg                                                                                                                               >  1:1999520/1‑150 (MQ=255)

                      cGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGaca                                                                                                                            >  2:1791524/1‑150 (MQ=255)

                         acacATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACAt                                                                                                                         >  1:1647784/1‑150 (MQ=255)

                              tCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGCTAAAACTGACACATTACTg                                                                                                                    >  1:1694962/1‑150 (MQ=255)

                               ttttGCTGCCATCGGCGGGAATAATGAAGCAAAATCT‑T‑TTAGCCTCTTTAACCCCCTTAC‑CCTGCCTTTTAATCTTTCCCATTTAAAAAATTCT‑TTAGTTTTTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGc                                                                                                                   <  2:2222139/149‑1 (MQ=255)

                               cTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGc                                                                                                                   >  1:3246833/1‑150 (MQ=255)

                                    ctgcCATCAGCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAg                                                                                                              >  2:1191956/1‑150 (MQ=255)

                                      gcCATCAGCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGc                                                                                                            >  1:1135710/1‑150 (MQ=255)

                                       ccATCAGCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCa                                                                                                           >  2:2003755/1‑150 (MQ=255)

                                        cATCAGCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCAc                                                                                                          >  2:3150066/1‑150 (MQ=255)

                                            aGCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAat                                                                                                      >  2:2901765/1‑150 (MQ=255)

                                             gCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAata                                                                                                     >  2:4186080/1‑150 (MQ=255)

                                             gCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAata                                                                                                     >  2:3873505/1‑150 (MQ=255)

                                               ggaaatAATGAAGCAAAACCT‑T‑TTAGCCTTGTTG‑ACCCCTTAACCCTCCCTTTTAATC‑TTGCCTATTAAAAAATTCCTTTAGTTTTTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAAtata                                                                                                   <  2:4073762/145‑1 (MQ=255)

                                               cGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAAtata                                                                                                   >  2:3790444/1‑150 (MQ=255)

                                                ggAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATaa                                                                                                  >  2:3915484/1‑150 (MQ=255)

                                                 gagaAATGAAGCAAAACCT‑T‑TTAGCCTCTTTAACCCACTTAC‑CCTGACTTTTAATTTTTCCCATTAAAAAAATTCC‑TTAGTTTTTTTTCTCCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAg                                                                                                 <  2:2421695/150‑1 (MQ=255)

                                                    taataaACCAAAAACC‑T‑TTAGACTCGTTAAACCACTTAA‑CCTGACCTTAAATCTTTGCCATTTAAAAAATTCT‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCt                                                                                              <  2:3339203/150‑1 (MQ=255)

                                                    tAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCt                                                                                              >  2:4132352/1‑150 (MQ=255)

                                                     aaTGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTc                                                                                             >  2:2537239/1‑150 (MQ=255)

                                                     aaTGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTc                                                                                             >  2:2791481/1‑150 (MQ=255)

                                                     aaTGAAGCAAAATCT‑T‑TTAGACTCGTTGAACCATTTAC‑CCTGTCTTTTAATCTTTGCCATTAAAAAAATTCT‑TTAGTTTTTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTc                                                                                             <  2:1354825/150‑1 (MQ=255)

                                                     aaTGAAG‑CAAAACC‑TCTTAGACTCGTCAAACCACTTAC‑CCTGACTTTTAATCTTTGCGATTTAAAAAATTCT‑TTAGATTTTTTTCTCCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTc                                                                                             <  2:3602575/150‑1 (MQ=255)

                                                         aaGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCa                                                                                         >  1:768555/1‑150 (MQ=255)

                                                         aaGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCa                                                                                         >  2:3831561/1‑150 (MQ=255)

                                                         aaGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCCCGGCa                                                                                         >  2:4352321/1‑150 (MQ=255)

                                                            ccAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCagag                                                                                      >  2:2700200/1‑150 (MQ=255)

                                                             cAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAgaga                                                                                     >  1:1304064/1‑150 (MQ=255)

                                                             aaaaaCC‑T‑TTAGACTTGTAGAACACCTAAA‑CCTGTCCTTTATTTTTCGCATTTAAAAAAATTCC‑TTGTATTTTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAgaga                                                                                     <  2:840555/149‑1 (MQ=255)

                                                              aaaaCC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGaa                                                                                    >  1:935510/1‑150 (MQ=255)

                                                              aaaaCC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGaa                                                                                    >  2:329515/1‑150 (MQ=255)

                                                               aaaCC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAg                                                                                   >  1:1940946/1‑150 (MQ=255)

                                                                aaCC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGc                                                                                  >  2:3011159/1‑150 (MQ=255)

                                                                     t‑ttAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTa                                                                              >  2:4060172/1‑150 (MQ=255)

                                                                        tAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACCGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTAtt                                                                            >  2:260460/1‑150 (MQ=255)

                                                                        tAGACTCGTTAACCCACTAAA‑CCTGCCCTTTAATCTTTCCCATTTAAAAAATTCC‑TTAGTTTGTTTTCTCCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTAtt                                                                            <  2:3775695/150‑1 (MQ=255)

                                                                            cTCGTTGACCCACTTAA‑CCTGACCTTTAATCTTTGCAATTTAAAAAATTCT‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATtcaac                                                                        <  1:3976053/150‑1 (MQ=255)

                                                                             tCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATtcaacg                                                                       >  1:3025758/1‑150 (MQ=255)

                                                                              cGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATtcaacgt                                                                      >  2:562572/1‑150 (MQ=255)

                                                                                  gAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATtcaacgtcaac                                                                  >  2:209476/1‑150 (MQ=255)

                                                                                    aCCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTcaacgtcaacgt                                                                >  1:2641085/1‑150 (MQ=255)

                                                                                         ttAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTa                                                           >  1:2826844/1‑150 (MQ=255)

                                                                                          tAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTAc                                                          >  1:2685871/1‑150 (MQ=255)

                                                                                           aa‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACt                                                         >  2:894632/1‑150 (MQ=255)

                                                                                           aa‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCCGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACt                                                         >  1:1063356/1‑150 (MQ=255)

                                                                                              ccTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCa                                                       >  2:2679940/1‑150 (MQ=255)

                                                                                                ttccttttATTTTTGGCTATTTAAAAATTTCT‑TTAGTTTTTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAgg                                                     <  2:3440422/145‑1 (MQ=255)

                                                                                                tgccttttAATCTTTGCCATTTAAAAAATTCT‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAgg                                                     <  1:1235362/145‑1 (MQ=255)

                                                                                                  aCCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGAc                                                   >  1:3432718/1‑150 (MQ=255)

                                                                                                   cttttAATCTTTGCTATTAAAAAATTTCC‑TTAGATTTTTTTCTCCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGAct                                                  <  2:1797504/148‑1 (MQ=255)

                                                                                                    cTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGActt                                                 >  1:540785/1‑150 (MQ=255)

                                                                                                      ttAATCTTTGCCATTTAAAAAATTCT‑TTAGATTGTTTTCTCCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGActtct                                               <  1:514693/150‑1 (MQ=255)

                                                                                                           ttttCCCATTTAAAAAATTCT‑TTAGTTTTTTTTCTCCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACt                                          <  2:3329528/149‑1 (MQ=255)

                                                                                                             ttGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGa                                        >  2:1664355/1‑150 (MQ=255)

                                                                                                             ttGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGCATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGa                                        >  1:1279211/1‑150 (MQ=255)

                                                                                                               gCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGaaa                                      >  1:1363395/1‑150 (MQ=255)

                                                                                                               gCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGaaa                                      >  1:2025051/1‑150 (MQ=255)

                                                                                                                ccATTTGAAAAATTCC‑TTAGATTGTTTTTTGCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGaaaa                                     <  1:2336419/150‑1 (MQ=255)

                                                                                                                caattaaaaaatTTCT‑TTAGTTTTTTTCCTGCCCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGaaaa                                     <  2:1865003/143‑1 (MQ=255)

                                                                                                                 ttttaaaaaatTTCT‑TTATTTTTTTTTCTCCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAAt                                    <  2:1018652/144‑1 (MQ=255)

                                                                                                                 cATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAAt                                    >  2:1510478/1‑150 (MQ=255)

                                                                                                                 cATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAAt                                    >  1:2324422/1‑150 (MQ=255)

                                                                                                                 cATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAAt                                    >  1:1611224/1‑150 (MQ=255)

                                                                                                                   tttGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGc                                  >  1:4285507/1‑150 (MQ=255)

                                                                                                                     tGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACCGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCca                                >  1:2551128/1‑150 (MQ=255)

                                                                                                                        aaaaTTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCcacac                             >  1:4306558/1‑150 (MQ=255)

                                                                                                                         aaaTTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCcacaca                            >  2:680115/1‑150 (MQ=255)

                                                                                                                           aTTCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATa                          >  2:2155349/1‑150 (MQ=255)

                                                                                                                            ttCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATaa                         >  1:1803659/1‑150 (MQ=255)

                                                                                                                             tCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATaaa                        >  1:2407805/1‑150 (MQ=255)

                                                                                                                             tCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATaaa                        >  1:3378427/1‑150 (MQ=255)

                                                                                                                             tCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATaaa                        >  2:4263623/1‑150 (MQ=255)

                                                                                                                             tCC‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATaaa                        >  1:2964118/1‑150 (MQ=255)

                                                                                                                              tcttaaGATTGTTTTCTGCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATaaa                        <  1:689898/145‑1 (MQ=255)

                                                                                                                              cc‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAAc                       >  1:3556936/1‑150 (MQ=255)

                                                                                                                               c‑TTAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACa                      <  1:4062324/150‑1 (MQ=255)

                                                                                                                                 ttAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAg                     >  1:4173773/1‑150 (MQ=255)

                                                                                                                                  tAGATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGAGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGa                    >  2:458034/1‑150 (MQ=255)

                                                                                                                                    gATTGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAAc                  >  1:812444/1‑150 (MQ=255)

                                                                                                                                       tGTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGt               >  2:1415449/1‑150 (MQ=255)

                                                                                                                                        gTTTTCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTa              >  1:3481352/1‑150 (MQ=255)

                                                                                                                                         ttttCTTCGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTAc             >  2:1745357/1‑150 (MQ=255)

                                                                                                                                               tcGCCCGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGtt       <  2:227991/150‑1 (MQ=255)

                                                                                                                                               ccgcccgccGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGtt       <  2:2447097/149‑1 (MQ=255)

                                                                                                                                                    cGCCGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAAcc  <  2:2643481/150‑1 (MQ=255)

CCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACC‑T‑TTAGACTCGTTGAACCACTTAA‑CCTGACCTTTAATCTTTGCCATTTGAAAAATTCC‑TTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACGTCAACGTGTTTACTCAGGACTTCTTTACTGAAAATGCCACACATAAACAGAACTGTACCTCGTTTAACC  >  AP012306/1587469‑1587762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`