Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306202,4170TG25.8% 522.5 / 34.3 230V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base T (74/96);  new base G (59/0);  total (134/96)
Fisher's exact test for biased strand distribution p-value = 1.05e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 279 total aligned reads displayed.

TTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACCG  >  AP012306/202269‑202566
                                                                                                                                                    |                                                                                                                                                      
   aaaGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGAttt                                                                                                                                                    >  1:3355261/1‑150 (MQ=255)
     aGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGGCGGGGGAttttt                                                                                                                                                  >  2:34806/1‑148 (MQ=255)
        gcCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGAc                                                                                                                                               >  1:1014780/1‑150 (MQ=255)
           aGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGGAGGGGGATTTTTGGCGCa                                                                                                                                            >  2:1893391/1‑150 (MQ=255)
           aGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCa                                                                                                                                            <  2:3714938/150‑1 (MQ=255)
            ggATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCaa                                                                                                                                           <  1:3011489/150‑1 (MQ=255)
                 gctgcATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGGAGGGGGATTTTTGACGCaaaaaag                                                                                                                                      >  2:1589986/1‑148 (MQ=255)
                 gctgcATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTTTGACGCCAGAAgg                                                                                                                                      >  1:3128698/1‑150 (MQ=255)
                   tgcATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGgtg                                                                                                                                    >  1:2147585/1‑150 (MQ=255)
                    gcATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGGTTTTTGGCGCCAAAAggggg                                                                                                                                   >  2:1338892/1‑147 (MQ=255)
                     cATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGtgtg                                                                                                                                  <  2:4116545/150‑1 (MQ=255)
                      atatTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCCAGAAGGTGTgg                                                                                                                                 >  1:2599656/1‑150 (MQ=255)
                       tatTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGGAGGGGGATTTTTGGCGCCAGAAGGGGTggg                                                                                                                                >  2:2605810/1‑149 (MQ=255)
                       tatTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGc                                                                                                                                >  1:2254010/1‑150 (MQ=255)
                          tGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑a                                                                                                                            <  2:1422739/150‑1 (MQ=255)
                            tCGGGCGTAACGGCGGACGCAAGCTAAAGAAAATCTGGCAAGAGCTGGGGGGGCCGCCGTGGCTACGGGACACCACGGCAGTGCTGTTTTATGGCGAAACGCTGGTTGCGGCGGCAGGGGGGTTTGTGAGGGAAGAAGGGGGGGcgggag                                                                                                                           >  2:2586930/1‑145 (MQ=255)
                             cGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCCGGGGGATTTGTGACGCAAGAAGGTGTGGCTG‑AAgg                                                                                                                         >  1:838834/1‑150 (MQ=255)
                              gggCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGt                                                                                                                        >  1:276042/1‑150 (MQ=255)
                                       ggcggACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGc                                                                                                               >  1:1778333/1‑150 (MQ=255)
                                          ggACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTa                                                                                                            >  1:4128561/1‑150 (MQ=255)
                                           gACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGGGAGAATGGCGTaa                                                                                                           >  1:946277/1‑150 (MQ=255)
                                            aCGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAg                                                                                                          >  2:2893398/1‑150 (MQ=255)
                                            aCGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAg                                                                                                          >  1:1424534/1‑150 (MQ=255)
                                               tAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGttt                                                                                                       >  1:1496179/1‑150 (MQ=255)
                                               tAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGGGAGAATGGCGTAAGttt                                                                                                       >  1:2278464/1‑150 (MQ=255)
                                                aaGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGtttt                                                                                                      <  1:606630/150‑1 (MQ=255)
                                                aaGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGGGAGAATGGCGTAAGtttt                                                                                                      >  2:1717671/1‑150 (MQ=255)
                                                 aGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTg                                                                                                     >  1:2034011/1‑150 (MQ=255)
                                                  gCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGt                                                                                                    <  1:391221/150‑1 (MQ=255)
                                                   cTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGGCGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTc                                                                                                   >  1:1170910/1‑150 (MQ=255)
                                                    tAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCCGGGGGATTTGTGGCGCAAGAAGGTGTGGCTG‑AAGGGGAGAATGGCGTAAGTTTTGTCt                                                                                                  >  2:4217840/1‑150 (MQ=255)
                                                    tAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCt                                                                                                  <  1:188112/150‑1 (MQ=255)
                                                    tAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCt                                                                                                  >  1:4073009/1‑150 (MQ=255)
                                                    tAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCt                                                                                                  <  2:787261/150‑1 (MQ=255)
                                                      aaGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTgg                                                                                                <  2:965450/150‑1 (MQ=255)
                                                        gAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCa                                                                                              <  2:1819076/150‑1 (MQ=255)
                                                         aaaaTCTGGCAAGAGCTGGGCGTGCCGACGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGGGAGAATGGCGTAAGTTTTGTCTGGCAg                                                                                             >  2:2399369/1‑150 (MQ=255)
                                                            aTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGaaa                                                                                          <  2:894404/150‑1 (MQ=255)
                                                            aTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGaaa                                                                                          <  2:2185206/150‑1 (MQ=255)
                                                             tCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCCGGGGTATTTGTGACGCAAAAAGGTGTGGCCGTAAGGGGGGAA‑GGCGTAAGGTTTGTCTTGGCGaaaa                                                                                         >  2:1020083/1‑150 (MQ=255)
                                                             tCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGaaaa                                                                                         <  1:2460655/150‑1 (MQ=255)
                                                             tCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGaaaa                                                                                         >  1:3801469/1‑150 (MQ=255)
                                                               tGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACg                                                                                       <  1:2577316/150‑1 (MQ=255)
                                                               tGGCAAGAGCAGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACg                                                                                       <  1:3046622/150‑1 (MQ=255)
                                                                ggCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGc                                                                                      >  2:1639027/1‑150 (MQ=255)
                                                                ggCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGc                                                                                      >  2:4167832/1‑150 (MQ=255)
                                                                 gCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCt                                                                                     <  1:265400/150‑1 (MQ=255)
                                                                  cAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCCGGGAGATTTGTGACGCAAGAAGGGGTGGCTG‑AAGGTGGGCATGGGGTAAGTTTTGTCTGGCAGCAAACGCtt                                                                                    >  2:2728097/1‑150 (MQ=255)
                                                                  cAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCtt                                                                                    <  2:3607766/150‑1 (MQ=255)
                                                                   aaGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTa                                                                                   <  1:2970915/150‑1 (MQ=255)
                                                                    agagCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAg                                                                                  >  2:1060238/1‑150 (MQ=255)
                                                                        cTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTaa                                                                              >  1:1266120/1‑150 (MQ=255)
                                                                        cTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTaa                                                                              <  1:105964/150‑1 (MQ=255)
                                                                         tGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCCGGGGGATTTGTGAAGCAAGGAGGTGTGGCTG‑GAGGGGAGAAAAGGGTGAGTTTTGTCTGGGAGGAAAAGCTTGGTTAAg                                                                             >  2:1111404/1‑150 (MQ=255)
                                                                           ggCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTg                                                                           >  1:3169346/1‑150 (MQ=255)
                                                                           ggCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGGGTGGCTG‑AAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTg                                                                           >  2:2990441/1‑150 (MQ=255)
                                                                              gTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAActtacgc                                                                                  <  2:1946181/140‑7 (MQ=255)
                                                                              gTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGaaa                                                                        <  1:2210984/150‑1 (MQ=255)
                                                                                gccgccGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGc                                                                      <  2:1403019/150‑1 (MQ=255)
                                                                                gccgccGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGc                                                                      >  1:3938294/1‑150 (MQ=255)
                                                                                gccgccGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGATTTGTCTGGCAGAAAACGCTTAGTTAAGTGaaaac                                                                      >  1:790664/1‑148 (MQ=255)
                                                                                        ggCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAg                                                              >  1:2860137/1‑150 (MQ=255)
                                                                                        ggCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGGTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGGTAAg                                                              >  1:3420292/1‑150 (MQ=255)
                                                                                              gTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑GAGGTGAGAATGGCGTAAGTTTTTTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAAACGCAt                                                        >  2:37983/1‑150 (MQ=255)
                                                                                                gACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCa                                                      >  1:1971586/1‑150 (MQ=255)
                                                                                                   accacGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAAc                                                   <  2:2471001/150‑1 (MQ=255)
                                                                                                    ccacGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAACCGGATAAGAAGCATCAAACg                                                  >  2:3409773/1‑150 (MQ=255)
                                                                                                      acGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGGAAGGTTTGTCTGGCAGAAAACGCTTTGTTAAGTGAAAGCCGGGTAAGGCGCATCAAACGTc                                                >  2:1221812/1‑150 (MQ=255)
                                                                                                        gCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGc                                              <  1:4199368/150‑1 (MQ=255)
                                                                                                        gCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGc                                              >  1:1311579/1‑150 (MQ=255)
                                                                                                         ccACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAACCGGATAAGACGCATCAAACGTCGCa                                             >  1:3464561/1‑150 (MQ=255)
                                                                                                         ccACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTG‑GAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCa                                             >  2:3894715/1‑150 (MQ=255)
                                                                                                            ctgctgTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATcc                                          <  2:3742717/150‑1 (MQ=255)
                                                                                                            ctgctgTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATcc                                          >  2:2504785/1‑150 (MQ=255)
                                                                                                            ctgctgTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGGGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATcc                                          >  2:1973599/1‑150 (MQ=255)
                                                                                                             tgctgTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCg                                         <  1:454435/150‑1 (MQ=255)
                                                                                                                   tttATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGaaa                                   <  2:435903/150‑1 (MQ=255)
                                                                                                                     tATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGt                                 <  1:3698802/150‑1 (MQ=255)
                                                                                                                      aTGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTc                                >  2:3959200/1‑150 (MQ=255)
                                                                                                                         gCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAAt                             >  2:1136654/1‑150 (MQ=255)
                                                                                                                         gCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAAt                             <  2:3500359/150‑1 (MQ=255)
                                                                                                                          cGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATc                            <  1:1031907/150‑1 (MQ=255)
                                                                                                                           gAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCa                           >  2:3538484/1‑150 (MQ=255)
                                                                                                                             aaCGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAgg                         <  2:671903/150‑1 (MQ=255)
                                                                                                                              aCGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGa                        <  2:1537438/150‑1 (MQ=255)
                                                                                                                              aCGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGa                        >  1:2337980/1‑150 (MQ=255)
                                                                                                                               accttcttGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGAt                       >  2:932567/7‑150 (MQ=255)
                                                                                                                                gCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGAtt                      >  1:428783/1‑150 (MQ=255)
                                                                                                                                gCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGGTAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGAtt                      >  1:1686067/1‑150 (MQ=255)
                                                                                                                                 cTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTc                     <  1:758847/150‑1 (MQ=255)
                                                                                                                                 cTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTc                     >  2:3325132/1‑150 (MQ=255)
                                                                                                                                  tGATTGCGGCGGCCGGGGTATTTGTGACGCAAGAAGGGGTGGATG‑TAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGGAAGCCGGATAAGACGCCTCAAACGTCGCATCCGGCGAAAGGCAATCAGGATTCg                    >  2:2302103/1‑150 (MQ=255)
                                                                                                                                    aTTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCt                  >  1:1834412/1‑150 (MQ=255)
                                                                                                                                        cggcggCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTacc              >  2:3241022/1‑150 (MQ=255)
                                                                                                                                         ggcggcAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTacca             >  1:2395719/1‑150 (MQ=255)
                                                                                                                                            ggcAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTaccacca          >  1:241987/1‑150 (MQ=255)
                                                                                                                                              cAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTaccaccacc        >  1:3308558/1‑150 (MQ=255)
                                                                                                                                                 gggTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTa     >  1:375819/1‑150 (MQ=255)
                                                                                                                                                  ggTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTAc    >  2:504023/1‑150 (MQ=255)
                                                                                                                                                   gTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTAcc   <  1:1602114/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
TTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTG‑AAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACCG  >  AP012306/202269‑202566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑