Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,376,6120TG23.9% 449.4 / 26.0 180V326G (GTA→GGA) ygbNpredicted transporter
Reads supporting (aligned to +/- strand):  ref base T (36/101);  new base G (43/0);  total (79/101)
Fisher's exact test for biased strand distribution p-value = 6.00e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 253 total aligned reads displayed.

CCAATGGTAGCTCTAATGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTACCAGCCGCATTTATTATTTCACTGGCGCTGCGTGCATCGCAGGGGTCAGCAACCGTAGCAATACTGAC  >  AP012306/2376464‑2376759
                                                                                                                                                    |                                                                                                                                                   
ccAATGGTAGCTCTAATGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTTCTTGGGCTGGGGGGGTa                                                                                                                                                    >  1:1076617/1‑150 (MQ=255)
 cAATGGTAGCTCTAATGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTTGTTACTGGTGCTGGAGGGGGAt                                                                                                                                                   >  1:2860403/1‑150 (MQ=255)
  aaTGGTAGCTCTAATGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGGAtt                                                                                                                                                  >  1:2677412/1‑150 (MQ=255)
   aTGGTAGCTCTAATGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTAttt                                                                                                                                                 <  2:2916857/150‑1 (MQ=255)
      gTAGCTCTAATGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGc                                                                                                                                              <  1:2376326/150‑1 (MQ=255)
       tAGCTCTAATGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGGATTTGGCa                                                                                                                                             >  1:3706029/1‑150 (MQ=255)
       tAGCTCTAATGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGGGCTGGGGGGGGATTTGGCa                                                                                                                                             >  2:208169/1‑150 (MQ=255)
         gCTCTAATGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTTGTTACTGGTGCTGGGGGGGGATTTGGCaaa                                                                                                                                           >  1:3223926/1‑150 (MQ=255)
         gCTCTAATGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGGATTTGGCaaa                                                                                                                                           >  2:2662824/1‑150 (MQ=255)
          ctctAATGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAg                                                                                                                                          <  1:1036476/150‑1 (MQ=255)
          ctctAATGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTACTGATTTTTGTTACTAGGGGTTGAGGGGGGTTTggtagaa                                                                                                                                          >  1:1521616/1‑145 (MQ=255)
           tctAATGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGt                                                                                                                                         <  1:1608166/150‑1 (MQ=255)
            ctAATGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTg                                                                                                                                        <  1:1127458/150‑1 (MQ=255)
              aaTGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCt                                                                                                                                      <  2:224353/150‑1 (MQ=255)
                tGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCtgg                                                                                                                                    <  1:4237847/150‑1 (MQ=255)
                tGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGAGTTTTGGTACTGGTGCTGGAGGGGGATTTTGCAAAGTGCtgg                                                                                                                                    >  2:863890/1‑150 (MQ=255)
                tGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGGGGTTTTTGTTACTGGTGCTGGGGGGGGATTTGGCAAAGtggtgg                                                                                                                                    >  2:3841186/1‑150 (MQ=255)
                   ttGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCtggtgg                                                                                                                                 <  1:409160/150‑1 (MQ=255)
                   ttGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCtggtgg                                                                                                                                 <  1:775147/150‑1 (MQ=255)
                    tGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGa                                                                                                                                <  2:1103604/150‑1 (MQ=255)
                      cgcTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAAt                                                                                                                              <  2:625843/150‑1 (MQ=255)
                        cTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGGATTTGGCAAAGTGCTGGTGGGATCg                                                                                                                            >  2:3421190/1‑150 (MQ=255)
                         tGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGGAGGGATTTTTGTTACTGGTGGTGGAGGGGGATTTTGCAAAATGCTGGTGGGATCgg                                                                                                                           >  2:2735238/1‑150 (MQ=255)
                           gTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGc                                                                                                                         <  2:4223719/150‑1 (MQ=255)
                           gTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGGGATTTTTGTTACTGGTGCTGGAGGGGGATTTGGCAAAGTGCTGGTGGGATCGGGc                                                                                                                         >  1:1665652/1‑150 (MQ=255)
                              cTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCGCAGCGCTTCCTACTGCGGCGGTAGTGATTTTTGTTACTGGTGCTGGAGGGGGATTTTGCAAAGTGCTGGTGGGATCGGGCggc                                                                                                                      >  2:3222648/1‑148 (MQ=255)
                                ggCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTTGCAAAGTGCTGGTGGAATCGGGCGTCgg                                                                                                                    >  1:1200583/1‑150 (MQ=255)
                                ggCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCgg                                                                                                                    <  2:1162156/150‑1 (MQ=255)
                                 gCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGc                                                                                                                   <  2:3788285/150‑1 (MQ=255)
                                 gCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGc                                                                                                                   <  1:3413112/150‑1 (MQ=255)
                                   aTTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGGGGGGGATTTTGCAAAGTGCTGGGGGGATCGGGGGTCGGCaa                                                                                                                 >  2:1419566/1‑150 (MQ=255)
                                     tCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAg                                                                                                               <  2:1325928/150‑1 (MQ=255)
                                      cTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGGGGGGGATTTGGCAAAATGCTGGTGGGATCGGGCGGCGGCAAAGc                                                                                                              >  2:3528466/1‑150 (MQ=255)
                                       tGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGcc                                                                                                             <  2:533737/150‑1 (MQ=255)
                                         gTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGGGCTGGAGGGGGATTTTGCAAAGGGCTGGGGGGATCGGGCGTCGGCAAAGCCCt                                                                                                           >  2:4088097/1‑150 (MQ=255)
                                          ttATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCAGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCtt                                                                                                          <  2:3988527/150‑1 (MQ=255)
                                           tATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTg                                                                                                         <  2:2400126/150‑1 (MQ=255)
                                           tATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTg                                                                                                         <  2:1518536/150‑1 (MQ=255)
                                              tGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGGATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCa                                                                                                      >  2:460399/1‑150 (MQ=255)
                                               ggCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGGATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCCTGccca                                                                                                     >  1:1442357/1‑148 (MQ=255)
                                                gCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAAc                                                                                                    <  2:3075548/150‑1 (MQ=255)
                                                  tttACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACAt                                                                                                  <  2:1926907/150‑1 (MQ=255)
                                                  tttACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACAt                                                                                                  <  2:1637996/150‑1 (MQ=255)
                                                  tttACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGGATTTGGCAAAGTGCTGGTGGGATCGGGCGGCGGCAAAGCCCTTGCCAACaa                                                                                                  >  2:775502/1‑149 (MQ=255)
                                                   ttACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATg                                                                                                 <  1:1249482/150‑1 (MQ=255)
                                                    tACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGc                                                                                                <  2:2733562/150‑1 (MQ=255)
                                                      cGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGGAGGGATTTTTGTTTCTGGTGGTGGGGGGGGATTTTGGCAAGGGCTGGGGGGATCGGGGGTCGGCCAAACCCTTTCCAACATGCTa                                                                                              >  2:3357803/1‑150 (MQ=255)
                                                         cgcgGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACaa                                                                                           <  1:444520/150‑1 (MQ=255)
                                                           cgGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGGGCTGGGGGGGGATTTGGCAAAGGGCTGGTGGAATCGGGCGGCGGCAAAGCCCTTTCCAACATGCTACAAAt                                                                                         >  2:503133/1‑150 (MQ=255)
                                                            ggCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATg                                                                                        >  1:689537/1‑150 (MQ=255)
                                                            ggCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGGATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATg                                                                                        >  1:3651605/1‑150 (MQ=255)
                                                               tGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGAtt                                                                                     <  2:4100932/150‑1 (MQ=255)
                                                               tGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGGATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGAtt                                                                                     >  1:3976767/1‑150 (MQ=255)
                                                                ggAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTg                                                                                    <  1:2300101/150‑1 (MQ=255)
                                                                 gAGCTTACAACACACCAGCGACATTATTGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGGGGGGGATTTGGCAAAGTGCTGGTGGAATCGGGGGGCGGCAAAGCCCTTGCCAACAAGCTACAAATGATTGa                                                                                   >  2:2988224/1‑150 (MQ=255)
                                                                      tACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGGATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGc                                                                              >  1:3366867/1‑150 (MQ=255)
                                                                      tACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGGATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGc                                                                              >  1:1750936/1‑150 (MQ=255)
                                                                        caacaCACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGATACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCct                                                                            <  2:3433602/150‑1 (MQ=255)
                                                                          acacacCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCctct                                                                          <  1:2211785/150‑1 (MQ=255)
                                                                            acacCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGt                                                                        <  1:925143/150‑1 (MQ=255)
                                                                            acacCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGAAATGGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGt                                                                        <  2:899895/150‑1 (MQ=255)
                                                                             cacCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGtt                                                                       <  1:2438906/150‑1 (MQ=255)
                                                                              acCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGGGGGGGATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTa                                                                      >  2:2444680/1‑150 (MQ=255)
                                                                                cAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTAcc                                                                    <  2:2343708/150‑1 (MQ=255)
                                                                                 aGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGGTTTTGGGTACTGGTGCTGGAGGGGGATTTGGCAAAGTGCTGGTGGGATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTTCCa                                                                   >  2:740313/1‑150 (MQ=255)
                                                                                 aGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGGGATTTTTGTTTCTGGGGCTGGGGGGGGATTTTGCAAAATGCTGGGGGGAACGGGCGGCGGCCAAACCCCTTCCCACATGCTACAAAAGATTTACCCGCCCCCGtttaca                                                                   >  2:3155789/1‑146 (MQ=255)
                                                                                 aGCGACATTAGGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTACCa                                                                   <  2:3749368/150‑1 (MQ=255)
                                                                                  gCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTACCAg                                                                  <  1:2080138/150‑1 (MQ=255)
                                                                                   cGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTACCAGc                                                                 <  1:3549973/150‑1 (MQ=255)
                                                                                     aCATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTTGTTAATGGGGGTGGGGGGGGATTTGGCAAAGTGCTGGTGGGATCGGGCGGCGGCAAAACCCCTTCCAACATGCTACAAAAGATTTACCCTCCCCTGTTTCCAGCCg                                                               >  2:1027070/1‑150 (MQ=255)
                                                                                      cATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGGGGGGGATTTGGCAAAGTGCTGGGGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCCCTGTTTCCAGCCGc                                                              >  2:3881180/1‑150 (MQ=255)
                                                                                         tATGGGCTCAGCGCTTCCTCCTGCGGCGGTAGTGATTTTGGTTACGGGTGCTGGGGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAGCCCCTTGCCAACATGCTACAAATGATTGCCCTGCCTCTGTTACCAGCCGCAtt                                                           <  2:2306274/150‑1 (MQ=255)
                                                                                         tATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTACCAGCCGCAtt                                                           <  1:1781993/150‑1 (MQ=255)
                                                                                          aTGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAACCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTACCAGCCGCAttt                                                          <  2:79625/150‑1 (MQ=255)
                                                                                             ggCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGGGCTGGGGGGGGATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCCCTGTTACCAGCCGCATttatt                                                       >  2:1043480/1‑150 (MQ=255)
                                                                                              gcgaaGCGCTTCATACTGCGCAGGTAGTGATTTTGGTTATTGGTCCTGGAGGGGTATTTGGAAAAGTGCTGGTGGAACCGGGCGTCGGCAAAGCCCTTGCCAACATGCTAAAAATGATTGCCCTGCCTCTGTTACCAGCCGCATttatta                                                      <  2:2861693/146‑1 (MQ=255)
                                                                                              gCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTTGTTACTGGGGCTGGGGGGGGATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTACCAGCCGCATttatta                                                      >  2:755071/1‑150 (MQ=255)
                                                                                              gCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTACCAGCCGCATttatta                                                      <  2:2580123/150‑1 (MQ=255)
                                                                                              gCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTACCAGCCGCATttatta                                                      <  2:3630652/150‑1 (MQ=255)
                                                                                                tCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTAAAAATGATTGACCTGCCTCTGTTACCAGCCGCAtttgtagca                                                    <  2:1546253/150‑7 (MQ=255)
                                                                                                     ttttCCTACGGCGGGGTTAGGGATTTTGGTTACTGGTGCGGGAGGGGTATTTGGAAAGGTTCGGGGGGAATCGGGCGTCGGAAAGGCCCTTGCCAACATCCTAAAAATGATTGACCTGCCTCTGTTACCAGCCGCATTTATTATTTCACt                                               <  2:2052343/148‑1 (MQ=255)
                                                                                                     ggttCCTTCTGGGGCGGTGTTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAGTTGTTGGTGGAAGCGGGCGTCGCCAAGCCCCTTGCCAACATGCTACAAATAATTGACCTGCCTCTGTTACCAGCCGCATTTATTATTTCACt                                               <  2:2186441/148‑1 (MQ=255)
                                                                                                     gcTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGGGGGGGATTTTGCAAAGTGCTGGTGGGATCGGGCGGCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTACCAGCCGCATTTATTATTTCACt                                               >  2:3046123/1‑150 (MQ=255)
                                                                                                       ttCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTACCAGCCGCATTTATTATTTCACTgg                                             <  1:1969753/150‑1 (MQ=255)
                                                                                                         ccTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTACCAGCCGCATTTATTATTTCACTGgcg                                           <  1:3794083/150‑1 (MQ=255)
                                                                                                             cTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTACCAGCCGCATTTATTATTTCACTGGCGCTGc                                       <  1:212075/150‑1 (MQ=255)
                                                                                                                     gTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTACCAGCCGCATTTATTATTTCACTGGCGCTGCGTGCATCg                               >  2:1407930/1‑150 (MQ=255)
                                                                                                                          gATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTACCAGCCGCATTTATTATTTCACTGGCGCTGCGTGCATCGCAggg                          >  2:3589801/1‑150 (MQ=255)
                                                                                                                           aTTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTACCAGCCGCATTTATTATTTCACTGGCGCTGCGTGCATCGCAgggg                         <  2:3364893/150‑1 (MQ=255)
                                                                                                                           aTTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTACCAGCCGCATTTATTATTTCACTGGCGCTGCGTGCATCGCAgggg                         >  2:3955906/1‑150 (MQ=255)
                                                                                                                                  ttACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTACCAGCCGCATTTATTATTTCACTGGCGCTGCGTGCATCGCAGGGGTCAGCaa                  >  1:1318426/1‑150 (MQ=255)
                                                                                                                                  ttACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTACCAGCCGCATTTATTATTTCACTGGCGCTGCGTGCATCGCAGGGGTCAGCaa                  >  2:1750822/1‑150 (MQ=255)
                                                                                                                                    aCTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTACCAGCCGCATTTATTATTTCACTGGCGCTGCGTGCATCGCAGGGGTCAGCAAcc                >  1:2528071/1‑150 (MQ=255)
                                                                                                                                          gCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTACCAGCCGCATTTATTATTTCACTGGCGCTGCGTGCATCGCAGGGGTCAGCAACCGTAGCa          >  1:1899053/1‑150 (MQ=255)
                                                                                                                                            tGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTACCAGCCGCATTTATTATTTCACTGGCGCTGCGTGCATCGCAGGGGTCAGCAACCGTAGCAAt        >  1:739788/1‑150 (MQ=255)
                                                                                                                                              gAGGGTTATTTGGCAAAGTGCTGGTGAAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTACCAGCCGCATTTATTATTTCACTGGCGCTGCGTGCATCGCAGGGGTCAGCAACCGTAGCAATAc      <  2:986141/150‑1 (MQ=255)
                                                                                                                                               gggggTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTAAAAATGATTGCCCTGCCTCTGTTACCAGCCGCATTTATTATTTCACTGGCGCTGCGTGCATCGCAGGGGTCAGCAACCGTAGCAATACt     <  2:667238/149‑1 (MQ=255)
                                                                                                                                               gggggTATTTGGAAAAGTGCTGGTGGAATCGGGCGTCGGCAAGGCCTTTGCCAACAGGCTAAAAATGATTGACCTGCCTCTGTTACCAGCCGCATTTATTATTTCACTGGCGCTGCGTGCATCGCAGGGGTCAGCAACCGTAGCAATACt     <  2:1006453/149‑1 (MQ=255)
                                                                                                                                                ggggTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTACCAGCCGCATTTATTATTTCACTGGCGCTGCGTGCATCGCAGGGGTCAGCAACCGTAGCAATACTg    >  2:1248709/1‑150 (MQ=255)
                                                                                                                                                  ggTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTACCAGCCGCATTTATTATTTCACTGGCGCTGCGTGCATCGCAGGGGTCAGCAACCGTAGCAATACTGAc  >  1:803580/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCAATGGTAGCTCTAATGATTGCGCTGGTGCTGGCATTCTGGTTATTGGCTTTACGTCGCGGCTGGAGCTTACAACACACCAGCGACATTATGGGCTCAGCGCTTCCTACTGCGGCGGTAGTGATTTTGGTTACTGGTGCTGGAGGGGTATTTGGCAAAGTGCTGGTGGAATCGGGCGTCGGCAAAGCCCTTGCCAACATGCTACAAATGATTGACCTGCCTCTGTTACCAGCCGCATTTATTATTTCACTGGCGCTGCGTGCATCGCAGGGGTCAGCAACCGTAGCAATACTGAC  >  AP012306/2376464‑2376759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑