Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,560,2430TG23.7% 401.6 / 21.3 169N419T (AAC→ACC) pepPproline aminopeptidase P II
Reads supporting (aligned to +/- strand):  ref base T (51/78);  new base G (40/0);  total (91/78)
Fisher's exact test for biased strand distribution p-value = 1.04e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 201 total aligned reads displayed.

GGCAGCGCCCCGTGACTTAA‑CCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCC  >  AP012306/2560094‑2560392
                                                                                                                                                      |                                                                                                                                                     
ggCCGCCCCCCGTGACTTAA‑CCGGAAAATACCCACCCCCAGCGCCCCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGt                                                                                                                                                       <  2:2532753/150‑1 (MQ=255)
ggCAGCGCCCCGTGACTTAA‑CCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGt                                                                                                                                                       >  1:3219204/1‑150 (MQ=255)
gccAGCGCCCCGTGACTTAC‑CCGGAAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGt                                                                                                                                                       <  1:3421150/148‑1 (MQ=255)
      cccccGTG‑CTTAACCCGGAAAATCCCCCGCCCCGGCGCCGCCCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGt                                                                                                                                                 <  2:4121696/149‑1 (MQ=255)
       ccccGTGACTTAA‑CCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGtt                                                                                                                                                >  1:1739915/1‑150 (MQ=255)
        cccGTGACTTAA‑CCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTTGTTa                                                                                                                                               >  2:3428051/1‑150 (MQ=255)
         ccGTGACTTAA‑CCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGGTAc                                                                                                                                              >  1:2111377/1‑150 (MQ=255)
           gTGACTTAA‑CCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTActg                                                                                                                                            >  2:1534673/1‑148 (MQ=255)
           gTGACTTAA‑CCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCg                                                                                                                                            >  1:1932943/1‑150 (MQ=255)
              aCTTAC‑CCGGAAAATGGCCGGCGCCGGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGtt                                                                                                                                         <  2:3087707/150‑1 (MQ=255)
               ctaaa‑CCGGAAAATGCCCAGCCCCAGCGTCCCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGttt                                                                                                                                        <  2:3624405/147‑1 (MQ=255)
                      cGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTTCCGGGTTTCGTaa                                                                                                                                  >  1:1890946/1‑150 (MQ=255)
                          aaaTAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTTGTTAGCTGTTTCGGTGATtac                                                                                                                              >  1:2154061/1‑150 (MQ=255)
                             tAGCCAGCGCCGGCGCCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAAt                                                                                                                           <  2:1299240/150‑1 (MQ=255)
                                 cagcgccagcgTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGGTTCGGGAATCACAATgtcg                                                                                                                       >  2:3287626/1‑150 (MQ=255)
                                 cagcgccagcgTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGCGGGGGTTTCGTTTCCGGTTTCGGTAAACCCAATgttc                                                                                                                       >  2:4057580/1‑148 (MQ=255)
                                  agcgccagcgTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATgtcgt                                                                                                                      >  1:734894/1‑150 (MQ=255)
                                   gcgccggcgCCCCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATgtcgtc                                                                                                                     <  2:2250456/150‑1 (MQ=255)
                                       cggcgcCGCCCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCa                                                                                                                 <  2:3025327/148‑1 (MQ=255)
                                        agcgTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCaa                                                                                                                <  1:1390716/150‑1 (MQ=255)
                                          cgTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATa                                                                                                              <  2:2927649/150‑1 (MQ=255)
                                          cgTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGGTTCGGTAATCACAATGTCGTCTTCAATa                                                                                                              >  1:1688242/1‑150 (MQ=255)
                                              gcgcCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGaa                                                                                                          <  1:2644854/150‑1 (MQ=255)
                                               ggcccgccATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAAt                                                                                                         <  1:430687/149‑1 (MQ=255)
                                               cgcccgccATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAAt                                                                                                         <  1:3773647/150‑1 (MQ=255)
                                               cgcccgccATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAAt                                                                                                         <  1:644825/150‑1 (MQ=255)
                                               cccccgccATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAAt                                                                                                         <  1:3013177/148‑1 (MQ=255)
                                                  ccgccATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGcc                                                                                                      >  2:2467849/1‑150 (MQ=255)
                                                  ccgccATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGGCTTCAATACGAATGcc                                                                                                      >  2:1955812/1‑150 (MQ=255)
                                                   cgccATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGGGAGGGTTTCGTTACCGGTTTCGGTAATCACCAAGTCGCCTTCAATACGAATGCCg                                                                                                     >  2:1072243/1‑150 (MQ=255)
                                                     ccATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGAt                                                                                                   <  1:1006229/150‑1 (MQ=255)
                                                       aTGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATAc                                                                                                 <  2:2195727/150‑1 (MQ=255)
                                                       aTGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGGTTCGGGAATCACAATGTCGTCTTCAATACGAATGCCGATAc                                                                                                 >  1:4162469/1‑150 (MQ=255)
                                                         gccgccACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACcg                                                                                               <  2:837173/150‑1 (MQ=255)
                                                          ccgccACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACcgc                                                                                              <  1:1872402/150‑1 (MQ=255)
                                                              cACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCCATACGAATGCCGATACCGCGata                                                                                          >  1:1090594/1‑150 (MQ=255)
                                                              cACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGata                                                                                          >  2:3756301/1‑150 (MQ=255)
                                                                  gacgaTGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGt                                                                                      >  1:574044/1‑150 (MQ=255)
                                                                    cgaTGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGCTTTCGCTACCGGTTTCCGTAAACACCATCTCGGCTTCAAAACGAATGCCCGTACCGCGATATTGTTc                                                                                    >  2:1671733/1‑150 (MQ=255)
                                                                     gatgatTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGGAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCt                                                                                   >  2:1566958/1‑150 (MQ=255)
                                                                      atgatTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGCGGTTTTCGGTACCGGTTTCAGTTATCCCCATGTCGTCTTCAATACGAATGCCGCTACCGCGATATTGTTCtt                                                                                  >  2:3616924/1‑149 (MQ=255)
                                                                      atgatTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTg                                                                                  <  1:998776/150‑1 (MQ=255)
                                                                      atgatTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCAGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTg                                                                                  <  1:4260576/150‑1 (MQ=255)
                                                                        gatTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGc                                                                                >  2:1465978/1‑150 (MQ=255)
                                                                         atTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACTCTGGCGGAGAGGATTTCGTTACCGGCTTCGGTAATCACAATGTCCTCTTCCAAACAAATGCCGACACCGCGGTATTGtttcagca                                                                               >  2:3376398/1‑144 (MQ=255)
                                                                         atTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCa                                                                               >  1:773014/1‑150 (MQ=255)
                                                                           tACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACt                                                                             <  2:3731948/150‑1 (MQ=255)
                                                                              gCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCt                                                                          <  1:3652952/150‑1 (MQ=255)
                                                                               cTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTTGCACTTCTg                                                                         >  1:2517302/1‑150 (MQ=255)
                                                                               cTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTg                                                                         >  1:3449343/1‑150 (MQ=255)
                                                                                 cATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCa                                                                       <  1:567061/150‑1 (MQ=255)
                                                                                   ttGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGGTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTTGCACTTCTGCATc                                                                     >  1:2490689/1‑150 (MQ=255)
                                                                                    tGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATcc                                                                    <  2:1723219/150‑1 (MQ=255)
                                                                                     gCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCg                                                                   <  1:469328/150‑1 (MQ=255)
                                                                                     gCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTTATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGGTATTGTTCTGGCACTTCTGCATCCg                                                                   >  2:1429620/1‑150 (MQ=255)
                                                                                       tttCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGgc                                                                 <  1:481121/150‑1 (MQ=255)
                                                                                       tttCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGgc                                                                 <  1:272931/150‑1 (MQ=255)
                                                                                         tctcGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATTCCCATACCGCGATATTGTTCTGGCCCTTCTGCATCCGgcgc                                                               >  2:3303169/1‑150 (MQ=255)
                                                                                            cGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAat                                                            <  2:3793793/150‑1 (MQ=255)
                                                                                             gcagcaACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAata                                                           >  1:2569437/1‑150 (MQ=255)
                                                                                               agcaACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAAtata                                                         <  1:4050177/150‑1 (MQ=255)
                                                                                                 caACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACa                                                       >  1:4228680/1‑150 (MQ=255)
                                                                                                  aaCCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAAGTTTTTGTTACCCGTTTCGGGAATCACAATTTCGTCTTCAATACGCAAGCCGATACCGCGATATTGTTCTAGCACTTCTGCATCCGGGGCAATATTCaa                                                      >  2:552159/1‑149 (MQ=255)
                                                                                                     cATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGccc                                                   <  2:744095/150‑1 (MQ=255)
                                                                                                     cATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAAAGCCGATACCGCGATATTTTTCTTGCACTTCTTCATCCCGCGCCATATACAGccc                                                   >  2:3145679/1‑150 (MQ=255)
                                                                                                      aTCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCt                                                  <  1:2488135/150‑1 (MQ=255)
                                                                                                       tCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTg                                                 <  1:186132/150‑1 (MQ=255)
                                                                                                        cAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTgg                                                <  2:2693255/150‑1 (MQ=255)
                                                                                                        cAACGCTTCGATGTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAAAGTCGTCTTCAATACGAAAGCCGATACCGCGATATTTTTCTTGCACTTCTTCATCCGGCGCAATATACAACCCCgg                                                >  2:4196343/1‑150 (MQ=255)
                                                                                                          aCGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGAGGTGGGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGct                                              >  2:3703077/1‑150 (MQ=255)
                                                                                                            gCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGGGGGTTTCGGTTCCGGGTTCGGTAATCACAATGTCGTCTTCTATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCCTCCGGCGCAATATACAGCCCTGGctct                                            >  2:1701098/1‑150 (MQ=255)
                                                                                                             cTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTa                                           <  1:2236864/150‑1 (MQ=255)
                                                                                                               tCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGGTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTTGCTCTACg                                         >  1:2771185/1‑150 (MQ=255)
                                                                                                                 gATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGGCTTCAATACGAATGCCGATACCCCGGTATTTTTCTGGCACTTCTGCATCCGGCGCAATATTCAGCCCCGGCTCTACGGt                                       >  2:444070/1‑150 (MQ=255)
                                                                                                                  aTTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGGTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTc                                      >  1:327525/1‑150 (MQ=255)
                                                                                                                     tcttcCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATACACAGCCCTGGCTCTACGGTCAGt                                   >  1:2920913/1‑150 (MQ=255)
                                                                                                                       ttcCGGCTCTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTAc                                 <  1:1580507/150‑1 (MQ=255)
                                                                                                                           ggCTTTTTCACCACGCTGGCGGTTGGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATAAGAATTCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAACCCTGGCTCTACGGTTAGTATCATg                             >  2:15214/1‑150 (MQ=255)
                                                                                                                            gCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGc                            >  2:2781622/1‑150 (MQ=255)
                                                                                                                             cTTTTTCACCACGCTGGCGGTGAGGTTTTTGGTACCGTTTTTGGTAATTACCAAGTCGTCTTCAATACTAATTCCGACACCCCTACATTGTTATGGTACTTCTGCACCCAGCGTAATATACCGCCCCTGATCTACGCCCACTACCATGcc                           >  2:1579512/1‑150 (MQ=255)
                                                                                                                             cTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGGTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGcc                           >  2:1833457/1‑150 (MQ=255)
                                                                                                                              tttttCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGccc                          <  1:876334/150‑1 (MQ=255)
                                                                                                                               ttttCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCg                         <  1:2308909/150‑1 (MQ=255)
                                                                                                                               ttttCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGGCAGTACCATGCCCg                         >  1:3948510/1‑150 (MQ=255)
                                                                                                                               ttttCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGACGTCTTCAATACGAATGGCGAAACCGCGATATTGTTCTTGCACTTCTGCATCCGGCGCAATATACAGCCCCGGCTCTACGGTCAGTACCCTGCCCg                         >  2:3861386/1‑150 (MQ=255)
                                                                                                                                tttCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTTTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCgg                        <  2:2338660/150‑1 (MQ=255)
                                                                                                                                 ttCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGt                       <  1:2831785/150‑1 (MQ=255)
                                                                                                                                   caccacGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTTGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTc                     >  1:2412833/1‑150 (MQ=255)
                                                                                                                                       acGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGGAATCACAAAGTCGGCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATTCCCGGGTCCCGa                 >  2:2744042/1‑150 (MQ=255)
                                                                                                                                         gCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAAt               <  2:2455830/150‑1 (MQ=255)
                                                                                                                                           tGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCGGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGGACCATGCCCGGGTCCAGAATgc             >  2:1133836/1‑150 (MQ=255)
                                                                                                                                            ggCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATgcg            >  1:3932596/1‑150 (MQ=255)
                                                                                                                                            ggCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATgcg            >  2:3972838/1‑150 (MQ=255)
                                                                                                                                              cGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGcgcg          >  2:927861/1‑150 (MQ=255)
                                                                                                                                                 tGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCgcgagc       <  2:2371379/150‑1 (MQ=255)
                                                                                                                                                   aGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCgcgagcga     >  2:2116910/1‑150 (MQ=255)
                                                                                                                                                     gTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATc   >  2:3554043/1‑150 (MQ=255)
                                                                                                                                                     gTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATc   <  2:633422/150‑1 (MQ=255)
                                                                                                                                                      ttttCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATcc  >  2:1201490/1‑150 (MQ=255)
                                                                                                                                                      ttttCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATcc  >  1:1262326/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GGCAGCGCCCCGTGACTTAA‑CCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCC  >  AP012306/2560094‑2560392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑