Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,758,9090TG26.3% 570.1 / 15.6 225G33G (GGT→GGG) agaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
Reads supporting (aligned to +/- strand):  ref base T (41/124);  new base G (59/0);  total (101/124)
Fisher's exact test for biased strand distribution p-value = 2.70e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 311 total aligned reads displayed.

AAGCGCAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGA  >  AP012306/2758760‑2759058
                                                                                                                                                     |                                                                                                                                                         
 aGCGCAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGtt                                                                                                                                                          <  1:3640410/150‑1 (MQ=255)
        ccGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTg                                                                                                                                                   <  1:1153970/150‑1 (MQ=255)
         cGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGgtttttcttg                                                                                                                                                  >  2:916298/1‑144 (MQ=255)
          gCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGGGGGTTTTCCTtgg                                                                                                                                                 >  2:1991994/1‑150 (MQ=255)
           cAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGc                                                                                                                                                <  1:3179417/150‑1 (MQ=255)
            aGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCTGGGCg                                                                                                                                               >  1:4039700/1‑150 (MQ=255)
              cccGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGAt                                                                                                                                             <  1:2201260/150‑1 (MQ=255)
              cccGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCCGGGCGAt                                                                                                                                             >  1:1249269/1‑150 (MQ=255)
                 gTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑tt                                                                                                                                         <  1:3213154/150‑1 (MQ=255)
                  ttGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTg                                                                                                                                        <  2:3223658/150‑1 (MQ=255)
                      cAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTCCTGGGCGAAG‑TTGCCgg                                                                                                                                    >  2:1760386/1‑149 (MQ=255)
                       aGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAt                                                                                                                                   <  2:4215924/150‑1 (MQ=255)
                       aGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCCTGGGGGTG‑TTGCCGAt                                                                                                                                   >  2:2944905/1‑150 (MQ=255)
                         tAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑cc                                                                                                                                <  2:3488649/150‑1 (MQ=255)
                          aaaaaTGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCTGGGCGATG‑TTGCCGCT‑CC‑t                                                                                                                              >  2:2113445/1‑150 (MQ=255)
                           aaaaTGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGCT‑CC‑T‑g                                                                                                                            >  1:478953/1‑150 (MQ=255)
                           aaaaTGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTCCCGGGCGGTG‑TTTCCGAT‑cccg                                                                                                                              >  2:1072535/1‑148 (MQ=255)
                                gaagaaGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCTGGGGGATG‑TTGCCCAT‑CC‑T‑Gaaaaa                                                                                                                       >  2:399045/1‑150 (MQ=255)
                                gaagaaGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑Gaaaaa                                                                                                                       >  2:1632556/1‑150 (MQ=255)
                                  agaagaGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGa                                                                                                                     <  2:2838436/150‑1 (MQ=255)
                                  agaagaGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTCCTGGGCGATG‑TTGCCCGT‑CC‑T‑TAAAAAGa                                                                                                                     >  2:3669018/1‑150 (MQ=255)
                                  agaagaGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTCCCGTGCGATG‑TTTCCGGT‑CC‑T‑GCAAAAGa                                                                                                                     >  1:3116201/1‑150 (MQ=255)
                                  agaagaGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTCCCGGGCGATG‑TTTGCGGT‑CC‑T‑GAAAAAGa                                                                                                                     >  2:1100501/1‑150 (MQ=255)
                                    aagaGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGAtt                                                                                                                   >  2:332115/1‑150 (MQ=255)
                                     agagGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGAttt                                                                                                                  >  2:256461/1‑150 (MQ=255)
                                      gagGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTta                                                                                                                 <  1:1454738/150‑1 (MQ=255)
                                      gagGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTta                                                                                                                 >  1:3541580/1‑150 (MQ=255)
                                       agGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTtat                                                                                                                <  1:2638353/150‑1 (MQ=255)
                                              cAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTTCTGGGGGGTG‑TTGCCGATCCC‑T‑GAAAAAAATTT‑TAAAgacg                                                                                                         >  2:2025643/1‑150 (MQ=255)
                                              cAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTACTTGGCGGTG‑TTGCCGGT‑CCTT‑GAAAAAAATTTTTAAgacg                                                                                                          >  2:31192/1‑147 (MQ=255)
                                                gCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGgacgac                                                                                                       <  1:1845731/150‑1 (MQ=255)
                                                  aaTGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACaa                                                                                                     <  1:3371248/150‑1 (MQ=255)
                                                  aaTGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTCCCAGGAGAGG‑TTTACGCA‑CC‑C‑GGAAAAAATTTATAAAgaggcaaa                                                                                                     >  2:220355/1‑144 (MQ=255)
                                                  aaTGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCTGGGGGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACaa                                                                                                     >  2:2702848/1‑150 (MQ=255)
                                                   aTGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACaaa                                                                                                    >  2:572661/1‑150 (MQ=255)
                                                     gggATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAAcc                                                                                                  <  2:2633409/150‑1 (MQ=255)
                                                     gggATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAAcc                                                                                                  <  1:2388960/150‑1 (MQ=255)
                                                       gATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGGT‑CC‑T‑GAAAAAGATTTATAAGGAAGACAAAACgg                                                                                                >  1:444360/1‑150 (MQ=255)
                                                       gATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCgg                                                                                                <  1:1340179/150‑1 (MQ=255)
                                                       gATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAAGACGACAAAcccg                                                                                                >  1:3006977/1‑148 (MQ=255)
                                                        aTCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCggg                                                                                               <  1:4092403/150‑1 (MQ=255)
                                                        aTCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCGGGGCGGTG‑TTGGCTGG‑CC‑TCAAAAAAAATTTTTAAGGA‑GGCAAACCcgg                                                                                               >  2:3034716/1‑150 (MQ=255)
                                                              aaTCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTCCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAg                                                                                         >  2:1781901/1‑150 (MQ=255)
                                                              aaTCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCTGGGCGATG‑TTGCCCAT‑CC‑T‑GAAAAAGAATTTTAAGGGCGACAAAACCGGCCTaaa                                                                                         >  2:1472798/1‑149 (MQ=255)
                                                                  aGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGcgcg                                                                                     >  1:1677326/1‑150 (MQ=255)
                                                                      aaaaaGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGa                                                                                 <  1:2875101/150‑1 (MQ=255)
                                                                      aaaaaGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCCGGGCGATG‑TTGCCCAT‑CC‑C‑GGAAAAGATTTATTAAGGCGACAAACCGGGGTTAAACCCGGCGa                                                                                 >  2:1210677/1‑150 (MQ=255)
                                                                       aaaaGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTTCCTGGGCGATG‑TTGCCGGT‑CC‑T‑GAAAAAAATTTATAAGGACGACAAAACGGGCTTAAGCGCGGCGAt                                                                                >  2:412825/1‑150 (MQ=255)
                                                                       aaaaGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCCGGGGGATG‑TTGCCGGG‑CC‑T‑GAAAAAGATTTATAAAGACGACAAACCAGGCGTAAGCgggggggt                                                                                >  2:1413227/1‑146 (MQ=255)
                                                                            atatCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGGT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGGTGAAAg                                                                           >  2:21748/1‑150 (MQ=255)
                                                                             tatCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGa                                                                          <  1:280965/150‑1 (MQ=255)
                                                                             tatCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGa                                                                          <  1:2425448/150‑1 (MQ=255)
                                                                               tCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTGCCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATa                                                                        <  1:2982977/150‑1 (MQ=255)
                                                                                 aCCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGGAAGATAAc                                                                      >  1:3446503/1‑150 (MQ=255)
                                                                                 aCCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAAc                                                                      <  1:759150/150‑1 (MQ=255)
                                                                                 aCCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTCCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGGAAGATAAc                                                                      >  1:2545043/1‑150 (MQ=255)
                                                                                  cccGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCTGGGGGGTG‑TTGCCGAT‑CC‑T‑GAAAAAAATTTTTAAGGACGACAAAACCGGCTTAAGCGCGGCGGTGAAAGATaaac                                                                     >  2:3401652/1‑148 (MQ=255)
                                                                                    cGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTc                                                                   <  1:3066591/150‑1 (MQ=255)
                                                                                     gTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTTCCTGGGGGGTG‑TTGCCGGT‑CC‑T‑GGAAAAGATTTATAAGGACGACAAACCGGGGTTAAACGCGGCGGTGAAAGAAAAACTCg                                                                  >  2:535629/1‑150 (MQ=255)
                                                                                      tCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGa                                                                 >  1:1605552/1‑150 (MQ=255)
                                                                                        tGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGGGGGTTTACCGAGGCCATT‑ATGCCCAA‑CC‑T‑GAAAAAGATTTTTAAGGACCCCAACCCGGGCTTAAGCGCGGCGGTTAAAGATAACCCCGAAt                                                               >  2:680948/1‑150 (MQ=255)
                                                                                          ggCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAAttt                                                             <  2:2812611/150‑1 (MQ=255)
                                                                                           gCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATtta                                                            <  2:2410130/150‑1 (MQ=255)
                                                                                            cTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATttat                                                           <  1:4266231/150‑1 (MQ=255)
                                                                                               tcgttcgtCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGGGGGTTTCACTGGGCGAGG‑TGGGCCGT‑CC‑T‑GAAAAAGGTTTATAAAGACGGCAAACCCGGGGTAAGCGCGGCGGTGAAAGAGAACCTCGAATTTATTaa                                                        >  2:4030613/1‑150 (MQ=255)
                                                                                                 gttcgtCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCCGGGCGATG‑TTGCCGCA‑CC‑T‑TAAAAAGAATTTTAAGGGAGACAAACCCGGGCTAAGCGCGGCGATGAAAAATAAACTCCAAATTTTTaaaa                                                      >  2:889819/1‑148 (MQ=255)
                                                                                                    cgtcgCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATaccc                                                   <  1:3826830/150‑1 (MQ=255)
                                                                                                     gtcgCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATaccca                                                  <  1:3257140/150‑1 (MQ=255)
                                                                                                      tcgCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATacccac                                                 <  2:229363/150‑1 (MQ=255)
                                                                                                       cgCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATacccacc                                                <  1:873431/150‑1 (MQ=255)
                                                                                                        gctgctGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATacccaccc                                               >  1:1759354/1‑150 (MQ=255)
                                                                                                              gcAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCt                                         <  1:1328896/150‑1 (MQ=255)
                                                                                                               cAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCGCCCGAATCTg                                        <  2:1867125/150‑1 (MQ=255)
                                                                                                               cAAGCGAGCTTTAACTACGAAAGGACGCAGGCGGGCGGGTTTACCTGGGCGATG‑TTTCCGAT‑CC‑T‑GAAAAAGATTTATAAAGACGACAAACCGGGCTTAAGCGCGGCGGTGAAAGATAACCTCGAATTTATTAATACCCCCCCGAATCTg                                        >  2:3876967/1‑150 (MQ=255)
                                                                                                                aaGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTgg                                       <  1:1014988/150‑1 (MQ=255)
                                                                                                                 aGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTTCTGGGCGGGGTTTGCCG‑T‑CC‑T‑GAAAAAAAGTTGTAAGGGAGCAAAACCGGGGGTTAGCGCGGGGGGGGAAGATAAACTCGAATTTTTTAATAACCACCCCAATTTGGt                                      >  2:617670/1‑150 (MQ=255)
                                                                                                                    gAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTCCTGGGGGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGGAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCgg                                   >  2:2366341/1‑150 (MQ=255)
                                                                                                                     aGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAAATTATTAATACCCACCCGAATCTGGTCGGa                                  >  1:3721824/1‑150 (MQ=255)
                                                                                                                       cTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTTCCTGGGGGATG‑TTTCCGAT‑CC‑T‑GAAAAAGATTTTTAAAGGAGACAAAACGGGCTTTAGCGCGGGGGTGAAAGATAACCCCGGATTTTTTAATAACCACCCGCATCTGGTCGGGtt                                >  2:1715214/1‑150 (MQ=255)
                                                                                                                       cTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTCCCGGGCGATG‑TTTCCGCT‑CC‑T‑GAAAAAAATTTTTAACGACGACAAACCGGGGTTTAGCGCGGCGGAGAAAAATAAACTCGAAATTATTAAAACCCACCCCAATCTTGGTGGAtt                                >  2:3011360/1‑150 (MQ=255)
                                                                                                                         ttAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTcc                              <  1:4071657/150‑1 (MQ=255)
                                                                                                                           aaCTACGAAAGGATGCAGGCGGTGGGCTTTTTCTCGGGGATG‑GTGGCGGA‑CC‑T‑CAAAAAGATTTATAAGGTCGGCAAAACGGGCGTAAGGCCGGAGGTGGAAAATAACCTCGGAGTTGTTATTACCCACCCGAATGCGGGCGGACTcccg                            >  2:3731023/1‑148 (MQ=255)
                                                                                                                           aaCTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTg                            <  1:3557904/150‑1 (MQ=255)
                                                                                                                           aaCTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTg                            <  1:674840/150‑1 (MQ=255)
                                                                                                                               aCGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATgg                        <  2:3572360/150‑1 (MQ=255)
                                                                                                                                    aGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGtta                   <  1:804228/150‑1 (MQ=255)
                                                                                                                                     ggATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGttat                  <  2:4227313/150‑1 (MQ=255)
                                                                                                                                          cAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAAtt             >  1:525882/1‑150 (MQ=255)
                                                                                                                                           aGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAAttt            >  1:2127001/1‑150 (MQ=255)
                                                                                                                                             gcgggcggTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCg          <  1:2030330/150‑1 (MQ=255)
                                                                                                                                               gggcggTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGAt        <  1:2675524/150‑1 (MQ=255)
                                                                                                                                                 gcggTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATgg      <  1:4112957/150‑1 (MQ=255)
                                                                                                                                                  cggTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGga     <  1:968214/150‑1 (MQ=255)
                                                                                                                                                   ggTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGgaa    >  1:607683/1‑150 (MQ=255)
                                                                                                                                                   ggTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGgaa    >  2:1299661/1‑150 (MQ=255)
                                                                                                                                                    gTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGgaag   >  1:3430231/1‑150 (MQ=255)
                                                                                                                                                     ttttACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGgaaga  <  1:2312806/150‑1 (MQ=255)
                                                                                                                                                     ttttACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGgaaga  <  1:911344/150‑1 (MQ=255)
                                                                                                                                                     ttttACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGgaaga  <  2:754570/150‑1 (MQ=255)
                                                                                                                                                     ttttACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGgaaga  >  1:1511266/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                         
AAGCGCAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGTTTTACCTGGGCGATG‑TTGCCGAT‑CC‑T‑GAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGA  >  AP012306/2758760‑2759058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑