Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,182,8610AC26.9% 286.9 / 28.1 119L198F (TTA→TTC) yiaNpredicted transporter
Reads supporting (aligned to +/- strand):  ref base A (76/11);  new base C (0/32);  total (76/43)
Fisher's exact test for biased strand distribution p-value = 5.72e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 210 total aligned reads displayed.

TTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGC‑A‑TGATGATGGGCGCAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGC  >  AP012306/3182713‑3183010
                                                                                                                                                                |                                                                                                                                                       
ttattCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTTCCCCCTGC‑A‑TGATTATTGGGGCAACGCTT‑AT‑GC‑TTA‑CC‑T‑GGTGGGGGGAGGCCA‑GGCC‑CCCTTA‑TTTaa                                                                                                                                                        >  2:505451/1‑149 (MQ=255)
 tattCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTTCCCCTGGG‑G‑TGGTGGTGGGCGCAAAGCTC‑AT‑GC‑TTT‑TC‑T‑GGGGGGGGCGGGGCC‑GTTG‑CCTTTA‑TTTAcc                                                                                                                                                       >  2:2887753/1‑150 (MQ=255)
  attCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGGATTTCCCCCCGC‑C‑TGATTATGGGCGCAACGCTG‑AT‑GC‑TTT‑CC‑T‑GGGGGGGGGGGGCCC‑GTCG‑CCCTTA‑TTTAccc                                                                                                                                                      >  2:115384/1‑150 (MQ=255)
  attCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCCTTTCCCCCGGG‑A‑CGATGCTGGGTGCGACGCCG‑GC‑GC‑TTT‑TC‑T‑GGTGGGGGCAGGCCC‑GGCG‑TCTtta‑cttactc                                                                                                                                                      >  2:536868/1‑148 (MQ=255)
    tCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGGATTGCCCCCGGC‑G‑TGATTATTGGGGGAAAGCCG‑AT‑TC‑TTA‑CC‑T‑GGGGGGGGGAGGCCA‑GGCGCCCTTAT‑TTTAcccc                                                                                                                                                     >  2:438838/1‑150 (MQ=255)
    tCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTTGCCCCCGC‑C‑TGATTATGGGGGCAACGCTG‑AT‑GT‑TTA‑CC‑T‑GGTGGTGGGAGGCCC‑GGCG‑CCTTTA‑TTTTTCCCg                                                                                                                                                    >  2:2783165/1‑150 (MQ=255)
       cccTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGGGTTGGCCCCGGG‑A‑CGAGGGGGGGGGGAAAGCTG‑AT‑GG‑TTT‑CC‑T‑GGGGGGGGGGGGGGA‑GTCG‑GCTTTA‑ATTTCCCCGccc                                                                                                                                                 >  2:1780868/1‑149 (MQ=255)
        ccTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGC‑A‑TGATGATGGGCGCAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTACCCCGCCAg                                                                                                                                                <  1:2173883/150‑1 (MQ=255)
         cTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGGATTTCCCCCGGG‑G‑TGATGATTGGGGCAACGCCG‑GT‑TC‑TTA‑CC‑T‑TGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTTCCCCCCCAGc                                                                                                                                               >  2:2040900/1‑150 (MQ=255)
         cTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTTCCCCCGGC‑A‑TGATGGTGGGGGCAACGCTG‑AT‑GC‑TTT‑CC‑T‑TGGGGGGGCGGGGCC‑GTCG‑CCTTTA‑TTTTCCCCCCCCGc                                                                                                                                               >  2:1410282/1‑150 (MQ=255)
          ttCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGGGTTGGCCCCGGC‑G‑TGGTGATGGGGGGGACGCTG‑GT‑GG‑TTT‑CC‑T‑GGTGGGGGGGGGGCC‑GGCG‑CCCTTA‑TTTTACCCGCCCGCa                                                                                                                                              >  2:726842/1‑150 (MQ=255)
             cATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCCTTTCCCCCCGC‑C‑TGATGATGGGGGCAACGCCG‑AT‑GC‑TTACCC‑T‑GGGGGGGGGGGGCCA‑GCGG‑CCTT‑A‑TTTTCCCCCCCCGCaaaa                                                                                                                                           >  2:2485660/1‑150 (MQ=255)
               ttCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCGTTGCCCCTGGC‑G‑TGGTGATGGGCGCCACCCCGTAT‑CC‑TTC‑CC‑C‑GG‑GGGGGGGGGCCA‑AGCG‑CCTTGA‑TTTACCCCCCCCGCAAAAAg                                                                                                                                         >  2:2720816/1‑150 (MQ=255)
                tCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTCGC‑A‑TGATTATGGGCGGAACGCTG‑AT‑GC‑TTT‑CC‑T‑GGTGGTGGGAGGGCA‑GTCG‑CCTTAA‑TTTATCCCCCCAGCaaaaaaa                                                                                                                                        >  2:1305072/1‑148 (MQ=255)
                 ccGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGC‑A‑TGATGATGGGCGCAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCa                                                                                                                                       <  1:775916/150‑1 (MQ=255)
                   gTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCCTTGCCCCCGGC‑C‑TGATGGTGGGGGCAACGCTG‑AT‑GT‑TTT‑CCTT‑GGGGGGGGGGGGCCG‑GCGC‑CCTTAT‑TTTTCCCCCCCGCCAAAAAGaaa                                                                                                                                      >  2:1727555/1‑150 (MQ=255)
                     ttattaTCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCCTTGCCCCCGGC‑G‑TGATGGTGGGGGCAAAGCTG‑AT‑GC‑TTT‑CC‑T‑GGTGGGGGGAGGGCC‑GTCG‑CCTTTA‑TTTTTCCCGCCCGCAAAAAGCAACGa                                                                                                                                   >  2:3863542/1‑150 (MQ=255)
                      tattatCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCGATGCCCCTCGC‑G‑TGGGGATGGGGGGGAAGCTG‑GT‑GC‑TTT‑CC‑T‑CGTGGTGGCAGGGCA‑GGGC‑CCTTTA‑TTTTCCCCCCCCGCCAAAAACAACgcg                                                                                                                                  >  2:1367544/1‑148 (MQ=255)
                      tattatCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCCCTGTCCCTCGC‑C‑CTGTGGTTGGCGCAAAACTG‑AT‑GCTTTA‑CC‑A‑GG‑GGTGGCAAGCCC‑ATTG‑CCCTTA‑TTTACCCCCCCCGCCAAAAAAAACgct                                                                                                                                  >  2:2966260/1‑148 (MQ=255)
                        ttatCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCCTTTCCCCCGGC‑C‑TGGTGATGGGCGCGAAGCAA‑TG‑GC‑GTT‑CC‑T‑GGTGGTGGGGGGGCA‑CGCG‑CCCTAA‑TTTTCCCCCCCCGCCAAAAGCCACGAAGc                                                                                                                                >  2:3296358/1‑150 (MQ=255)
                        ttatCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTTCCCCCGGC‑A‑TGATGATGGGCGCCACGCTG‑GT‑GC‑TTT‑CC‑T‑GGTGGGGGCGGGGCC‑GGCG‑CCCTTA‑TTTTTCCCCCCCGCCAAAAACAAAGAtac                                                                                                                                >  2:3131321/1‑148 (MQ=255)
                          atCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTAGGGC‑GGGCATTGCCCCTGGC‑A‑TGATGAGGGGCGCAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGGGGGGGCAGGCCA‑GTCG‑CCTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAg                                                                                                                              <  2:463460/150‑1 (MQ=255)
                            cTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGGATTGTCCCCCGG‑A‑TGGTGTTGGGGGGAACGGTG‑GTGGT‑TTT‑CC‑T‑GG‑GGGGGGGGGCCAGGTGG‑CCTT‑A‑TTTATCCCCCCAGCCAAAAACAAACATggagga                                                                                                                            >  2:2022100/1‑150 (MQ=255)
                             ttCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGGGTTGCCCCCCGC‑A‑TGATGATGGGGGCAACGCTG‑TT‑GC‑TTT‑TC‑T‑TGTGGGGGGAGGGCC‑GTCG‑CCTTTA‑TTTTACCCGCCCGCCAAAAACAACGATGCAggga                                                                                                                           >  2:3631197/1‑148 (MQ=255)
                             ttCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGGATTGCCCCCGGC‑A‑TGATGATGGGCGCAACGCTG‑AT‑GC‑TTT‑CC‑T‑GGTGGGGGGAGGCCC‑GGCG‑CCTTTA‑TTTTTCCCCCCAGCAAAAAGCAACGATGCCgggg                                                                                                                           >  2:3457883/1‑148 (MQ=255)
                             ttCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGC‑A‑TGATGATGGGCGCAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGGGGGGGCAGGCCA‑GTCG‑CCTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGaa                                                                                                                           <  2:2381690/150‑1 (MQ=255)
                               cGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGGATTTGCCCTTGG‑G‑TGGTTAAGGGGGCCAAGGCG‑TG‑GC‑TTT‑CC‑TGGGGGGGGGCGGGCCG‑GCGG‑CTTTAA‑TTTTCCCC‑CCAGCCAAAAACAACGATGTATGaaaa                                                                                                                         >  2:829564/1‑149 (MQ=255)
                               cGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGC‑A‑TGATGATGGGCGCAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAAt                                                                                                                         <  1:3687890/150‑1 (MQ=255)
                                 gCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTTCCCCCGGC‑C‑TGATGATGGGGGCAACGCTG‑AT‑GC‑TTT‑CC‑T‑GGGGGGGGGAGGGCA‑GTCG‑CCCTTA‑TTTACCCCCCCCGCGAAAAACAACGAAGCAGGGAAACt                                                                                                                       >  2:3565427/1‑150 (MQ=255)
                                 gCGTTCCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCGTGGC‑A‑TGATGATGGGCGCAACGCTG‑AT‑GC‑TTA‑CC‑G‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAT‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCt                                                                                                                       <  2:2130076/150‑1 (MQ=255)
                                   gTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGC‑A‑TGATGATGGGCGCAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CTTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTgg                                                                                                                     <  1:4018717/150‑1 (MQ=255)
                                       ccAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGGCATT‑CCCCCGGC‑A‑TGATGATGGGGGCGACGCTG‑AT‑GA‑TTA‑CC‑T‑GGGGGGGGGAGGCCC‑GTGG‑CCTTAA‑TTTTTCCCCCCCGCAAAAAAAAAAGAATGAGGGAAACTGGCAc‑t                                                                                                                >  2:953264/1‑150 (MQ=255)
                                         aGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGC‑A‑TGATGATGGGCGCAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAc‑tct                                                                                                              <  1:1637170/150‑1 (MQ=255)
                                            ggATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTTGCCCCGGC‑A‑TGATGATTGGGGCAACGCTG‑AT‑GG‑TTT‑CC‑T‑GGGGGGGGGGGGCCC‑GTCG‑GCCTTA‑TTTTCCCCCCCCGCAAAAAACAACGATGGAGGAAAAATGGCAC‑TCtttt                                                                                                           >  2:1214321/1‑150 (MQ=255)
                                            ggATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTTCCCCCGGC‑C‑TTATGATGGGGGCAACGCTG‑AT‑GC‑TTA‑CC‑T‑TGGGGGGGCGGGGCA‑GTCG‑CCCTTA‑TTTATCCCCCCCGCAAAAAAAAACGAAGCAAGAAAACTTGCAC‑CCtttt                                                                                                           >  2:2562559/1‑150 (MQ=255)
                                                tATCCATCAGTAAGCTGTTTATGGC‑GGGCATTTTCCCTGGC‑C‑TGTTTATGGGGGCAACGCTG‑AT‑GC‑TTT‑CC‑C‑GGTGGGGGCGGGCCC‑GTCG‑CCCTAA‑TTTACCCCCCCCCCAAAAAACAACGATGCAAGGAATCTGGCCC‑CCtttttttt                                                                                                       >  2:3800278/1‑146 (MQ=255)
                                                 aTCCATCAGTAAGCTGTTTATGGC‑GGGGATTTCCCCCCGC‑A‑TGATGATGGGGGCAACGCTG‑AT‑GG‑TTA‑CC‑C‑GGTGGGGGGCGGCCA‑GTCG‑CCCTTA‑TTTAACCCCCCCGCAAAAAACAAAGATGCAGGAAAACTGGCAC‑TCttttttttc                                                                                                      >  2:614756/1‑145 (MQ=255)
                                                    cATCAGTAATCTGTTTATGGG‑GGGCATTGCCCCGGGC‑A‑TGATGAGGGGCGAACCGCTG‑AT‑GC‑TTA‑CC‑T‑GGGGGTGGCGGGCCA‑GTCG‑CTTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCgg                                                                                                   <  2:359441/150‑1 (MQ=255)
                                                     aTCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCCCGC‑C‑TGATGATGGGGGCAACGCTG‑AT‑GC‑TTA‑CC‑C‑GGGGGGGGGCGGCCC‑AACG‑CCTTTA‑TTTACCCCCCCCCCCAAAAAAAAAGATGCCGGAAATATTGCAC‑TCTTTTTTTTcccgg                                                                                                  >  2:1558784/1‑147 (MQ=255)
                                                        aGTAAGCTGTTTATGGC‑GGGGATTGCCCCCGGC‑C‑AGATGATGGGGGGGACGCCG‑AT‑GG‑TTA‑CC‑T‑GGGGGGGGGAGGGCC‑GCCG‑CCCTAA‑TTTATCCCGCCCGCAAAAAACAACGAGGGAGGAAAAATGGCAC‑CCTTTTTTTTCCGGTAtt                                                                                               >  2:2198046/1‑149 (MQ=255)
                                                         ggaaGCTGTTTAGGGC‑GGCCA‑TGGCCCGGGC‑ATTGGTGATGGGCACAACCCGA‑AT‑CT‑TTA‑CG‑G‑GGTGGTGGCGGGCCA‑TGTG‑CCTTAT‑TTCACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCt                                                                                              <  2:3274932/148‑1 (MQ=255)
                                                          tAAGCTGTTTATGGC‑GGGCATTGCCCCCCGC‑A‑TGATGATGGGGGCAAAGCCG‑AT‑GC‑TTT‑CC‑T‑GGGGGTGGGAGGCCA‑GTCG‑CCTTTA‑TTTACCCCCCCCGCCAAAAACAACGAATCAGGGAAAATGGGACTTCTTTTTTTTCC‑GTATCtt                                                                                             >  2:3098920/1‑149 (MQ=255)
                                                               tGTTTATGGC‑GGGCATTGCCCCCGGC‑A‑TGATGATGGGGGCAACGCTG‑AT‑GC‑TTT‑CC‑T‑GGGGGGGGGAGGGCC‑GTCG‑CCCTAA‑TTTTTCCCCCCCGCAAAAAACAAAGATGCAGGGAAACTGGCAC‑TCTTTTTTTTCCCGTATCTGGgcgc                                                                                        >  2:2513177/1‑150 (MQ=255)
                                                                 tttATGGC‑GGGCATTGCCCCCCGC‑A‑TGATGATGGGGGCAAAGCTT‑AT‑GC‑TTT‑CC‑C‑CGGGGGGGGGGGCCA‑GTCG‑CCCTTA‑TTTACCCCCCCCGCCAAAAACAAAGAAGCAGGGAAACTGGGAC‑TCTTTTTTTTCCCGTATCTGGGGGCTg                                                                                      >  2:673647/1‑150 (MQ=255)
                                                                  ttATGGC‑GGGCATTGCCCCGGGC‑A‑TGATGATGGGCGCAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGt                                                                                     <  1:539143/150‑1 (MQ=255)
                                                                  ttATGGC‑GGGCATTGCCCCCTGC‑A‑TGTTGGTTGGGGCAACGCCG‑AT‑GG‑TTA‑CC‑T‑GGGGGTGGGAGGGCA‑GTTG‑CCTTTA‑TTTAACCCCCCCGCGAAAAACAAGGGCGCACGGAAACTGGCAG‑TCTTTTTTTTCCCGTGTCTTGGGGCTGt                                                                                     >  2:37632/1‑150 (MQ=255)
                                                                     tGGC‑GGGCATTGCCCCTGGC‑A‑TGATGATGGGCGCAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTttc                                                                                  >  2:3924315/1‑150 (MQ=255)
                                                                      ggC‑GGGCATTGCCCCTGGC‑A‑TGATGATGGGCGCAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTttct                                                                                 >  1:1433163/1‑150 (MQ=255)
                                                                             cATTGCCCTTGGC‑A‑TGATGATGGGCGCAGCGCTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGt                                                                           <  1:2336537/150‑1 (MQ=255)
                                                                             cATTGCCCCTGGC‑A‑TGATGATGGGCGCAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGt                                                                           <  1:331661/150‑1 (MQ=255)
                                                                               ttGCCCCTGGC‑A‑TGATGATGGGCGCAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtca                                                                         >  2:845668/1‑150 (MQ=255)
                                                                               ttGCCCCGGGA‑A‑TGATGAGGGGCGCAACGTTG‑TG‑GT‑TTC‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtca                                                                         <  2:2568470/150‑1 (MQ=255)
                                                                                 tccccTGGGAA‑TGATGAGGGGCGCACCGCTG‑AT‑GC‑TTC‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CTTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcat                                                                        <  2:4068843/149‑1 (MQ=255)
                                                                                 gCCCCTGGC‑A‑TGATGATGGGCGCAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcatc                                                                       >  1:1894279/1‑150 (MQ=255)
                                                                                  ccccTGGC‑A‑TGATGATGGGCGCAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcatca                                                                      >  2:676238/1‑150 (MQ=255)
                                                                                  ccccTGGC‑A‑TGATGATGGGCGCAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcatca                                                                      >  1:240348/1‑150 (MQ=255)
                                                                                  ccccTGGC‑A‑TGATGATGGGCGCAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGAAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtcatca                                                                      >  1:2711293/1‑150 (MQ=255)
                                                                                   ccgtGGC‑A‑TGATGGTGGCCCCAACGCTG‑AT‑TC‑TTC‑CC‑T‑GGTGGGGGCAGGCCA‑GTCG‑CTTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTcatcat                                                                     <  2:2452243/147‑1 (MQ=255)
                                                                                     cTGGC‑A‑TGATGATGGGCGCAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCatta                                                                   >  1:3675328/1‑150 (MQ=255)
                                                                                      gggC‑A‑TGATGAGGGGCGAAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CTTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCattat                                                                  <  1:3588107/149‑1 (MQ=255)
                                                                                           a‑tgatgatgGGCGCAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CTTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGc                                                              <  1:4057942/150‑1 (MQ=255)
                                                                                           a‑tgatgatgGGCGCAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGc                                                              >  2:3178166/1‑150 (MQ=255)
                                                                                                tgatgGGCGCAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCgggg                                                          >  1:4117881/1‑149 (MQ=255)
                                                                                                  atgGGCGCAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGgtt                                                        >  1:1309738/1‑150 (MQ=255)
                                                                                                   tgGGCGCAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTc                                                       >  1:3399726/1‑150 (MQ=255)
                                                                                                   ggggCGCAGCGCTG‑AT‑GC‑TTC‑CC‑T‑GGTGGTGGCGGGCCA‑GTCG‑CCTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTc                                                       <  2:4027734/149‑1 (MQ=255)
                                                                                                    gggCGCAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGgtttg                                                      >  2:3724514/1‑148 (MQ=255)
                                                                                                     ggCGCAACGCTG‑AT‑GA‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATATGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCggggtgcct                                                     >  2:3978769/1‑144 (MQ=255)
                                                                                                       cgcAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTGCTc                                                   >  1:3750189/1‑150 (MQ=255)
                                                                                                        gcAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTcc                                                  <  1:2923901/150‑1 (MQ=255)
                                                                                                              cTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCCGcccc                                            >  2:1666273/1‑148 (MQ=255)
                                                                                                               tG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGcccct                                           >  1:523956/1‑147 (MQ=255)
                                                                                                                     gC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTTCTCCCGCCTt‑ttttt                                      >  1:2304086/1‑148 (MQ=255)
                                                                                                                        ttA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTTCTCCCGCCCG‑TTTTccc                                    >  1:1667710/1‑150 (MQ=255)
                                                                                                                                gggggggggCAGGCCA‑GTCG‑CTTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTAcc                               <  2:800344/143‑1 (MQ=255)
                                                                                                                                       ggCAGGCCA‑GTCG‑CCTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCGAAGCCg                        <  2:573714/150‑1 (MQ=255)
                                                                                                                                        gCAGGCCA‑GTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCCCAGCCgg                       >  2:3262960/1‑150 (MQ=255)
                                                                                                                                         ggggtcA‑GTCG‑CTTAAG‑TTAACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCGAAGCCggg                      <  1:460146/145‑1 (MQ=255)
                                                                                                                                         cAGGCCA‑GTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTTCTCCGGCCCG‑TTTTTCCCCACCCAAACCcgg                      >  2:398087/1‑150 (MQ=255)
                                                                                                                                              cA‑GTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCGGCCTG‑TTTTTCCCTCCCCAAGCCGGGGCCgg                 >  1:1235426/1‑149 (MQ=255)
                                                                                                                                                 gTCG‑CCTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCGAAGCCGGGGCCGTtg               <  1:2769200/150‑1 (MQ=255)
                                                                                                                                                 gTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCGAAGCCGGGGCCGTtg               <  1:2125513/150‑1 (MQ=255)
                                                                                                                                                   cG‑CCTTAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCGAAGCCGGGGCCGTtgct             <  1:3922823/150‑1 (MQ=255)
                                                                                                                                                    g‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCGGGCCG‑TTTTCCCCTTCCCCAACCCGGGGCGtttttg            >  1:1209821/1‑146 (MQ=255)
                                                                                                                                                     cgcTTAATTTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGGCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCGAAGCCGGGGCCGTtgct             <  2:2217765/148‑1 (MQ=255)
                                                                                                                                                       cTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGCTCCCGCCCG‑TTTATCCCTACCCAAGCCGGGGCCGTTgctgct          >  1:3484366/1‑150 (MQ=255)
                                                                                                                                                       cTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGCTTCATCCCGCCCG‑TTTTCCCCCCCCGCAACCGCGGCCGGTggtgct          >  1:719934/1‑150 (MQ=255)
                                                                                                                                                       cTTAA‑TTCCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCGAAGCCGGGGCCGTTgctgct          <  1:1990167/150‑1 (MQ=255)
                                                                                                                                                        ttAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCCGCCCGCTTTACCCC‑ACCCACGCCCGGGGCGCTGcttctt         >  1:2976521/1‑150 (MQ=255)
                                                                                                                                                         tAA‑TTTCCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCGAAGCCGGGGCCGTTGCTGCttt        <  2:1448303/150‑1 (MQ=255)
                                                                                                                                                         tAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTTCTCCCGCCCG‑TTTTTCCCCACCCAAGCCCGGGGCGGTGCTGCttt        >  2:2069224/1‑150 (MQ=255)
                                                                                                                                                         tAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTTCTCCCGCCCG‑TTTTCCCCCACCCAAGCCGGGGGCGGTGCTGCttt        >  1:101991/1‑150 (MQ=255)
                                                                                                                                                          aa‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCGGCCCG‑TTTTCCCCTACCCCAGCCCGGGCCGTTGCTGCTTTc       >  1:2362163/1‑150 (MQ=255)
                                                                                                                                                          aa‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCCGCCCG‑TTTTCCCCTCCCCAAGCCGGGGCCGGTGCTGCTTTc       >  1:1136030/1‑150 (MQ=255)
                                                                                                                                                          aa‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGCTCCCGCCTG‑TTTTTCCCCACCCAAGCCCGGGGCGGTGCTGCtttt       >  1:843978/1‑149 (MQ=255)
                                                                                                                                                           a‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTGCTCCCGCCTG‑TTTTCCCCCACCCCAGCCCGGGGCGCTGCTGCttttt      >  1:2748809/1‑148 (MQ=255)
                                                                                                                                                               tACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGTCCCCGCCCC‑TTTTCCCCCACCCGCGCCGCGGCCGGTGCCCCCTTCTAtt   >  2:2950848/1‑149 (MQ=255)
                                                                                                                                                               tACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGCTTCGGCCCG‑TTTACCCCCACCGAAGCCGGGGCCGTTGCTGCTTTCTTTg   >  1:3641462/1‑150 (MQ=255)
                                                                                                                                                                aCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTTCTCCGGCCCG‑TTTTTCCCCCCCCCAGCCCGGGGCGGTGCTGCttttttttt  >  2:4209295/1‑144 (MQ=255)
                                                                                                                                                                aCCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCTCCCGCCTG‑GTTTCCCCTCCCGCAGCCCGGGGCGCGGCTGGTTTCttttc  >  1:3075559/1‑146 (MQ=255)
                                                                                                                                                                |                                                                                                                                                       
TTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGC‑GGGCATTGCCCCTGGC‑A‑TGATGATGGGCGCAACGCTG‑AT‑GC‑TTA‑CC‑T‑GGTGGTGGCAGGCCA‑GTCG‑CCTTAA‑TTTACCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCAC‑TCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTG‑TTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGC  >  AP012306/3182713‑3183010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑