Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,645,6420AC24.5% 837.0 / 29.4 326T318P (ACC→CCC) aceBmalate synthase A
Reads supporting (aligned to +/- strand):  ref base A (185/61);  new base C (0/80);  total (185/141)
Fisher's exact test for biased strand distribution p-value = 1.31e-37
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 361 total aligned reads displayed.

ATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCT  >  AP012306/3645493‑3645791
                                                                                                                                                      |                                                                                                                                                     
  atTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAAcc                                                                                                                                                     >  2:2307977/1‑150 (MQ=255)
  atTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAAcc                                                                                                                                                     >  1:1831015/1‑150 (MQ=255)
  atTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAAcc                                                                                                                                                     >  2:4014790/1‑150 (MQ=255)
   ttgttgGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCt                                                                                                                                                    >  1:2820627/1‑150 (MQ=255)
    tgttgGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTg                                                                                                                                                   <  2:4208673/150‑1 (MQ=255)
          tCTGAACTGCGGTGGTTGGGATTACATCTTCAGCTATAACAAAGCGTTGAAAATCTACCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCGGGAATGCTTCCTCACGCTTGTTGATTAAAACCTGCCATaa                                                                                                                                             <  2:2462869/150‑1 (MQ=255)
              aaCTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGAATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAAcgc                                                                                                                                         <  2:1312795/150‑1 (MQ=255)
                cTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCgg                                                                                                                                       >  1:637517/1‑150 (MQ=255)
                   cGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGACCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATCCCTGAATGCTTACTCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGc                                                                                                                                    <  1:2337747/150‑1 (MQ=255)
                     gTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCtt                                                                                                                                  >  1:226355/1‑150 (MQ=255)
                        gTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTg                                                                                                                               >  1:1588333/1‑150 (MQ=255)
                        gTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTg                                                                                                                               >  2:4263026/1‑150 (MQ=255)
                         ttGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGc                                                                                                                              >  1:2929311/1‑150 (MQ=255)
                          tGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCg                                                                                                                             >  1:1533424/1‑150 (MQ=255)
                          tGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTACCCCGATCTCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACCCCCGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCg                                                                                                                             <  2:2181953/150‑1 (MQ=255)
                              aTTACATCTTCAGCTATATAAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATgg                                                                                                                         <  1:1194895/150‑1 (MQ=255)
                                tACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGggc                                                                                                                       >  2:3910737/1‑150 (MQ=255)
                                 aCATCTTAAGTTAAATAAAACCGTGAAAAAACTACCCCGACCCCTTCCTGCCAGACAGAAGGGCAGGGACGAGGGAAAACCC‑ATCCCTAAATGCTTCCCCCCCCTTTTTGTTAAAACCCTGCCATAAACGCGGTGCTTTTGCGATGggcg                                                                                                                      <  2:3963734/150‑1 (MQ=255)
                                  cATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGggcgg                                                                                                                     >  2:2558426/1‑150 (MQ=255)
                                     cTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCAt                                                                                                                  >  1:2619277/1‑150 (MQ=255)
                                      ttaaGCTATATAAAAACGTTAAAAACCTACCCCGATCGCGCCCGGCCAGACAGACAGGCAGTGACGATGGAAAAACC‑ATCCCTAAATGCTTACTCCCGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATg                                                                                                                 <  2:1195859/147‑1 (MQ=255)
                                       tCAGTTATATCAACGCTTAGAAAAACTACCCCCATCGCGTCCTGCCAGCCAGCCAGGCAGTGACGTTGGATAAACC‑ATTCCTGAATTTTTCCCCCCTCCTGTTTTTTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATgg                                                                                                                <  2:1255772/150‑1 (MQ=255)
                                       tCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCCCGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATgg                                                                                                                <  1:2456600/150‑1 (MQ=255)
                                       tCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATgg                                                                                                                >  2:2140128/1‑150 (MQ=255)
                                        cAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATggc                                                                                                               >  2:599507/1‑150 (MQ=255)
                                        cAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATggc                                                                                                               >  1:210285/1‑150 (MQ=255)
                                        cAGCTATAAAAAAACGTTGAAAACCTACCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCCCGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATggc                                                                                                               <  2:2015206/150‑1 (MQ=255)
                                         aGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATggcg                                                                                                              >  2:2655707/1‑150 (MQ=255)
                                         aGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATggcg                                                                                                              >  1:4185051/1‑150 (MQ=255)
                                              tatCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGtt                                                                                                         >  2:1396035/1‑150 (MQ=255)
                                                tCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTa                                                                                                       <  1:3749775/150‑1 (MQ=255)
                                                   aaccGTTAAAAAACTACCCCGATCGGGTCCTGCCAGACAGACAGGCGGTGAGGATGAATAACCC‑ATTCCTAAATGCTTACTCGCGCTTTTTAATAAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTc                                                                                                    <  2:944716/147‑1 (MQ=255)
                                                    aaCGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTcc                                                                                                   >  1:3559851/1‑150 (MQ=255)
                                                    aaCGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTcc                                                                                                   <  1:1456467/150‑1 (MQ=255)
                                                    aaCGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTcc                                                                                                   >  1:4049611/1‑150 (MQ=255)
                                                    aaCGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTcc                                                                                                   >  1:3208807/1‑150 (MQ=255)
                                                    aaCGTTAAAAACCTATCCCGACCGCGTCCTGCCAAACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTAAATGTTTACCCACGCCTGTTGATAAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTcc                                                                                                   <  2:761078/150‑1 (MQ=255)
                                                       gTTGAAAAACTACCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAg                                                                                                <  1:1644343/150‑1 (MQ=255)
                                                          gAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCaa                                                                                             >  2:1885093/1‑150 (MQ=255)
                                                           aaaaaCTACCCCGACCGCGTCCTGCCAGACAGACAGGCAGGGACGATGGAAAACCC‑ATTCCTGAATGCTTCCTCCCGCTTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCaaa                                                                                            <  2:441382/150‑1 (MQ=255)
                                                             aaaCTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGa                                                                                          >  1:3974533/1‑150 (MQ=255)
                                                             aaaCTACCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGa                                                                                          <  1:3818162/150‑1 (MQ=255)
                                                              aaCTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGAt                                                                                         >  2:4104468/1‑150 (MQ=255)
                                                              aaCTACCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCCCGCCTGTTGATAAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGAt                                                                                         <  2:3823891/150‑1 (MQ=255)
                                                              aaCTACCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGAAAAACC‑ATTCCTGAATGCTTACTCCCGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGAt                                                                                         <  2:3493734/150‑1 (MQ=255)
                                                               aCTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATg                                                                                        >  1:1920552/1‑150 (MQ=255)
                                                               aCTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATg                                                                                        >  2:1363192/1‑150 (MQ=255)
                                                                cTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATTGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGa                                                                                       >  1:2699757/1‑150 (MQ=255)
                                                                 tATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCCCGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGaa                                                                                      <  1:1524752/150‑1 (MQ=255)
                                                                  aTCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAag                                                                                     >  2:2422091/1‑150 (MQ=255)
                                                                   tCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAaga                                                                                    >  1:714673/1‑150 (MQ=255)
                                                                   tCCCGATCGCGTCCTGCCAGAAAGACAGGAGGTGACGA‑GGAAAAACCAATCCCTAAATTCTTACTCACGCTTGTTGATAAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAaga                                                                                    <  2:3146201/150‑1 (MQ=255)
                                                                    cccGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAagag                                                                                   >  1:215876/1‑150 (MQ=255)
                                                                       gATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGcac                                                                                >  2:3533106/1‑150 (MQ=255)
                                                                       gATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGcac                                                                                >  2:2905227/1‑150 (MQ=255)
                                                                       gATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGcac                                                                                >  1:1750558/1‑150 (MQ=255)
                                                                       gATCGCGCCCTGCCAGACAGCCGGGCGGTGCCGATGGATAACCC‑ATCCCTGAATGCTTACCCCCCCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGcac                                                                                <  2:373591/150‑1 (MQ=255)
                                                                        aTCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACCCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGcaca                                                                               <  2:706163/150‑1 (MQ=255)
                                                                          cgcgTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAAt                                                                             >  2:1724766/1‑150 (MQ=255)
                                                                           gcgTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATa                                                                            >  1:1352194/1‑150 (MQ=255)
                                                                            cgTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTCCCCCCGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATaa                                                                           <  2:3002046/150‑1 (MQ=255)
                                                                            cgTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATaa                                                                           <  1:440947/150‑1 (MQ=255)
                                                                             gTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAAc                                                                          >  1:1983003/1‑150 (MQ=255)
                                                                              tCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAAcc                                                                         <  1:2564541/150‑1 (MQ=255)
                                                                                cTGCCAGACAGACAGGCAGTGACGATGGATAACCC‑ATTCCTGAATGCTTACTCACGCCTGTTGATAAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAg                                                                       <  2:2351749/150‑1 (MQ=255)
                                                                                  gcaagacagcaAGGAGGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGt                                                                     <  1:670725/147‑1 (MQ=255)
                                                                                     agacagacAGGCAGTGAGGATGGATAAACC‑ATTCCTGAATGCTTCCTCGCGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCt                                                                  <  2:1466655/150‑1 (MQ=255)
                                                                                     agacagacAGGAAGTGACGATGAAAAACCC‑ATTCCTGAATGCTTCCCCCCGCCTGTTGATAAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCt                                                                  <  2:306567/150‑1 (MQ=255)
                                                                                            cagGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACaaa                                                           <  1:3361330/150‑1 (MQ=255)
                                                                                              gggaGTGACGATGGAAAACCC‑ATTCCTAAATGCTTTCCCCCGCCTGTGGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGt                                                         <  2:1545950/147‑1 (MQ=255)
                                                                                               gCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTa                                                        <  1:3639694/150‑1 (MQ=255)
                                                                                                  gTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTaaaa                                                     <  2:3783450/150‑1 (MQ=255)
                                                                                                       gATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGa                                                <  1:98556/150‑1 (MQ=255)
                                                                                                        aTGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGAt                                               <  1:3696663/150‑1 (MQ=255)
                                                                                                        aTGGAAAAACC‑ATCCCTGAATGCTTCCCCCCGCCTGTGGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGAt                                               <  2:2026914/150‑1 (MQ=255)
                                                                                                         tGGATAAACC‑TTTCCGGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATa                                              <  1:1299692/150‑1 (MQ=255)
                                                                                                          ggATAAACC‑ATTCCTGAATGCTTACTCGCGCTTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATaa                                             <  1:2430738/150‑1 (MQ=255)
                                                                                                          gaaTAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATAAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATaa                                             <  1:1002623/148‑1 (MQ=255)
                                                                                                              aaaCC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCg                                         >  2:3340034/1‑150 (MQ=255)
                                                                                                               aaCC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGc                                        >  2:2807515/1‑150 (MQ=255)
                                                                                                                aCC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCAGAGCAAAGATGAAGACCACAATAACCCGATGCTCAACCAAGTAAAAGCGGATAAATCGCt                                       >  2:1817940/1‑150 (MQ=255)
                                                                                                                    aTTCCTGAATGCTTACTCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGa                                    <  1:3052063/150‑1 (MQ=255)
                                                                                                                     ttCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGaa                                   >  1:1249322/1‑150 (MQ=255)
                                                                                                                       ccTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGc                                 >  1:856356/1‑150 (MQ=255)
                                                                                                                        cTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGcc                                <  1:786083/150‑1 (MQ=255)
                                                                                                                          gAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCaa                              <  1:1131749/150‑1 (MQ=255)
                                                                                                                           aaTGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAAt                             >  2:343224/1‑150 (MQ=255)
                                                                                                                            aTGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATa                            >  1:342745/1‑150 (MQ=255)
                                                                                                                                 tACTCACGCTTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGt                       <  2:1646680/150‑1 (MQ=255)
                                                                                                                                 tACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGt                       >  2:2858333/1‑150 (MQ=255)
                                                                                                                                  aCTCACGCCTGTTGATTAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTc                      <  1:1627895/150‑1 (MQ=255)
                                                                                                                                      aCGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGa                  >  2:2720315/1‑150 (MQ=255)
                                                                                                                                              tGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACAt          >  2:2250004/1‑150 (MQ=255)
                                                                                                                                              tGATAAAACCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACAt          <  1:953156/150‑1 (MQ=255)
                                                                                                                                                aTTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGGAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACATgg        >  2:1425825/1‑150 (MQ=255)
                                                                                                                                                 taaaaaCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACATGGa       <  2:1077540/148‑1 (MQ=255)
                                                                                                                                                  tAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACATGGAt      >  2:3269519/1‑150 (MQ=255)
                                                                                                                                                  tAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACATGGAt      <  1:3908155/150‑1 (MQ=255)
                                                                                                                                                   aaaaCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACATGGATc     <  2:3664576/150‑1 (MQ=255)
                                                                                                                                                      aCCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCt  >  2:2271245/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
ATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATCTTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCAGTGACGATGGATAAACC‑ATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCATAAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAGCACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGTCACGATGGCACATGGATCGCT  >  AP012306/3645493‑3645791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑