BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	1,437,305	A→T	T9S (ACC→TCC)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	1,437,305	0	A	T	100.0%	1164.3 / NA	289	T9S (ACC→TCC)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (132/157); total (132/157)

Only 100 of 297 total aligned reads displayed.

TTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACT  >  AP012306/1437158‑1437451

  tCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCAt                                                                                                                                                >  2:2501282/1‑150 (MQ=255)

   cGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATc                                                                                                                                               >  1:2667502/1‑150 (MQ=255)

      tGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGa                                                                                                                                            <  1:1092943/150‑1 (MQ=255)

        tCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGAcc                                                                                                                                          <  1:349796/150‑1 (MQ=255)

         cACCTATCCTTAGAGCGAGGCACCACCACTTTCGTGATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCg                                                                                                                                         >  1:5027/1‑150 (MQ=255)

                ccTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGAGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCg                                                                                                                                  >  1:2079353/1‑150 (MQ=255)

                    agagCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATc                                                                                                                              >  2:3421562/1‑150 (MQ=255)

                     gagCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCt                                                                                                                             <  2:1551767/150‑1 (MQ=255)

                     gagCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCt                                                                                                                             <  2:794584/150‑1 (MQ=255)

                      agCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTg                                                                                                                            <  2:2427932/150‑1 (MQ=255)

                           ggCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAagag                                                                                                                       <  2:823065/150‑1 (MQ=255)

                              accaccacTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATg                                                                                                                    <  2:4093114/150‑1 (MQ=255)

                                 accacTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTa                                                                                                                 <  2:2602560/150‑1 (MQ=255)

                                   cacTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGc                                                                                                               >  1:3626996/1‑150 (MQ=255)

                                    acTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAGGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCt                                                                                                              <  2:1988374/150‑1 (MQ=255)

                                    acTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCt                                                                                                              <  2:1490470/150‑1 (MQ=255)

                                       ttCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTaaa                                                                                                           <  2:1620085/150‑1 (MQ=255)

                                        tCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTaaaa                                                                                                          <  2:3162955/150‑1 (MQ=255)

                                         cGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAAt                                                                                                         <  2:266932/150‑1 (MQ=255)

                                         cGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAAt                                                                                                         <  1:4063196/150‑1 (MQ=255)

                                         cGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAAt                                                                                                         >  1:374615/1‑150 (MQ=255)

                                            aaTACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCt                                                                                                      <  2:297248/150‑1 (MQ=255)

                                             aTACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTg                                                                                                     <  2:1873337/150‑1 (MQ=255)

                                                    aTTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCGCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTg                                                                                              >  1:3956661/1‑150 (MQ=255)

                                                       cGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCa                                                                                           <  2:3579699/150‑1 (MQ=255)

                                                        gCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCAt                                                                                          >  1:2447624/1‑150 (MQ=255)

                                                        gCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCAt                                                                                          <  1:891344/150‑1 (MQ=255)

                                                          tttCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGa                                                                                        <  2:4085456/150‑1 (MQ=255)

                                                          tttCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGATGGACGCTGGCATGa                                                                                        >  2:103421/1‑150 (MQ=255)

                                                            tCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAACCCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAAc                                                                                      <  1:633129/150‑1 (MQ=255)

                                                            tCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAAc                                                                                      <  2:1410133/150‑1 (MQ=255)

                                                               ggCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGCTGTTAGCTAAAATGCTGGACGCTGGCATGAACGtt                                                                                   >  2:2325898/1‑150 (MQ=255)

                                                               ggCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGtt                                                                                   <  1:1749409/150‑1 (MQ=255)

                                                                  aGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATg                                                                                >  1:729398/1‑150 (MQ=255)

                                                                   gTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGc                                                                               <  2:3952350/150‑1 (MQ=255)

                                                                   gTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGc                                                                               <  2:513988/150‑1 (MQ=255)

                                                                     gcgcCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGt                                                                             <  1:698408/150‑1 (MQ=255)

                                                                      cgcCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTc                                                                            >  2:325609/1‑150 (MQ=255)

                                                                       gcCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCt                                                                           <  2:3082875/150‑1 (MQ=255)

                                                                        cccAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTg                                                                          >  1:329688/1‑150 (MQ=255)

                                                                        cccAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTg                                                                          >  1:4201090/1‑150 (MQ=255)

                                                                        cccAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTg                                                                          <  2:2844394/150‑1 (MQ=255)

                                                                         ccAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGa                                                                         <  2:4021863/150‑1 (MQ=255)

                                                                           aGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAAc                                                                       <  2:1165839/150‑1 (MQ=255)

                                                                             aaaGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACtt                                                                     >  2:2733618/1‑150 (MQ=255)

                                                                                 cAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTtctct                                                                 <  1:1556747/150‑1 (MQ=255)

                                                                                   aGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCa                                                               >  2:1335587/1‑150 (MQ=255)

                                                                                    gTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCAt                                                              >  1:1846853/1‑150 (MQ=255)

                                                                                    gTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCAt                                                              >  1:2165397/1‑150 (MQ=255)

                                                                                      ttCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATgg                                                            >  1:1026777/1‑150 (MQ=255)

                                                                                       tctcCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGt                                                           >  2:1525722/1‑150 (MQ=255)

                                                                                         tcCGATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGa                                                         <  2:3305553/150‑1 (MQ=255)

                                                                                         tcCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGa                                                         >  2:2789551/1‑150 (MQ=255)

                                                                                           ccATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACt                                                       >  1:2139680/1‑150 (MQ=255)

                                                                                           ccATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACt                                                       <  1:3847619/150‑1 (MQ=255)

                                                                                           ccATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCAGCGGACCTAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTGATGCGTCTGAACTTCTCTCATGGTGACt                                                       <  2:611716/150‑1 (MQ=255)

                                                                                            cATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTa                                                      >  1:1331422/1‑150 (MQ=255)

                                                                                             aTCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGACCTTCTCTCATGGTGACTAt                                                     >  1:1370006/1‑150 (MQ=255)

                                                                                             aTCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTAt                                                     >  1:2066959/1‑150 (MQ=255)

                                                                                             aTCCTTCTCAACTTAAAGACTAAGACTGACATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTAt                                                     >  1:96186/1‑150 (MQ=255)

                                                                                                cTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCa                                                  <  1:827064/150‑1 (MQ=255)

                                                                                                   cgcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGACCTTCTCTCATGGTGACTATGCAGaa                                               <  2:3936840/148‑1 (MQ=255)

                                                                                                    tcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAac                                              >  1:2498268/1‑150 (MQ=255)

                                                                                                    tcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAac                                              >  1:255588/1‑150 (MQ=255)

                                                                                                    tcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAac                                              >  1:2593274/1‑150 (MQ=255)

                                                                                                      aaCTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAacac                                            <  1:2934373/150‑1 (MQ=255)

                                                                                                       aCTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACg                                           <  2:706647/150‑1 (MQ=255)

                                                                                                        cTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACgg                                          <  1:342524/150‑1 (MQ=255)

                                                                                                         ttAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGt                                         >  2:632728/1‑150 (MQ=255)

                                                                                                          tAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTc                                        <  1:3183109/150‑1 (MQ=255)

                                                                                                           aaaGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCa                                       <  1:2085155/150‑1 (MQ=255)

                                                                                                            aagactaagactGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAg                                      >  2:95484/1‑150 (MQ=255)

                                                                                                             agactaagactGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAgc                                     >  2:2348848/1‑150 (MQ=255)

                                                                                                             agactaagactGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAgc                                     <  2:1728905/150‑1 (MQ=255)

                                                                                                             agactaagactGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAgc                                     >  2:311383/1‑150 (MQ=255)

                                                                                                                ctaagactGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCa                                  >  1:2844962/1‑150 (MQ=255)

                                                                                                                 taagactGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCAt                                 >  2:2030407/1‑150 (MQ=255)

                                                                                                                 taagactGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCAt                                 >  2:3639680/1‑150 (MQ=255)

                                                                                                                   agactGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTc                               >  1:1269210/1‑150 (MQ=255)

                                                                                                                      ctGTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGa                            <  1:2481030/150‑1 (MQ=255)

                                                                                                                        gTCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAAt                          >  1:565144/1‑150 (MQ=255)

                                                                                                                         tCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATc                         <  1:3636449/150‑1 (MQ=255)

                                                                                                                         tCATGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATc                         <  1:166787/150‑1 (MQ=255)

                                                                                                                            tGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTgc                      <  2:2085917/150‑1 (MQ=255)

                                                                                                                            tGAAAAAGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTgc                      <  1:3663487/150‑1 (MQ=255)

                                                                                                                             gAAAAAGACCAAAATTGTTTGCCCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTgcg                     <  2:2936492/150‑1 (MQ=255)

                                                                                                                              aaaaaGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTgcgc                    >  1:2402469/1‑150 (MQ=255)

                                                                                                                                 aaGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAAc                 <  1:2776827/150‑1 (MQ=255)

                                                                                                                                  aGACCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACg                >  2:445860/1‑150 (MQ=255)

                                                                                                                                    aCCAAAATTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGtg              >  2:1241155/1‑150 (MQ=255)

                                                                                                                                       aaaaTTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGtgatg           <  2:3746098/150‑1 (MQ=255)

                                                                                                                                       aaaaTTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGtgatg           >  1:2587378/1‑150 (MQ=255)

                                                                                                                                        aaaTTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGtgatga          >  1:3571086/1‑150 (MQ=255)

                                                                                                                                        aaaTTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGtgatga          <  2:2162756/150‑1 (MQ=255)

                                                                                                                                         aaTTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAg         <  2:3458872/150‑1 (MQ=255)

                                                                                                                                         aaTTGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAg         <  1:296126/150‑1 (MQ=255)

                                                                                                                                           ttGTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCa       <  1:429051/150‑1 (MQ=255)

                                                                                                                                             gTTTGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACCTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCaaa     >  1:3533937/1‑150 (MQ=255)

                                                                                                                                              tttGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCaaaa    <  1:3011268/150‑1 (MQ=255)

                                                                                                                                              tttGCTCCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCaaaa    <  2:1459679/150‑1 (MQ=255)

TTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACT  >  AP012306/1437158‑1437451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`