Predicted mutation
evidence seq id position mutation annotation gene description
RA AP012306 1,587,321 G→C intergenic (‑115/+51) rrmA ← / ← cspC 23S rRNA m1G745 methyltransferase/stress protein, member of the CspA‑family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,587,3210GC99.7% 1483.5 / ‑4.5 366intergenic (‑115/+51)rrmA/cspC23S rRNA m1G745 methyltransferase/stress protein, member of the CspA‑family
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (205/160);  total (206/160)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 378 total aligned reads displayed.

AGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATT  >  AP012306/1587174‑1587467
                                                                                                                                                   |                                                                                                                                                  
agGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGc                                                                                                                                                  <  1:3285151/150‑1 (MQ=255)
    tGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTta                                                                                                                                              >  1:3035971/1‑150 (MQ=255)
       tGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAg                                                                                                                                           >  2:3743226/1‑150 (MQ=255)
         gCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCg                                                                                                                                         >  2:2188511/1‑150 (MQ=255)
         gCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCg                                                                                                                                         <  1:2061910/150‑1 (MQ=255)
          cAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACTCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCga                                                                                                                                        >  1:3590151/1‑150 (MQ=255)
            aaGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCgaga                                                                                                                                      <  2:1055309/150‑1 (MQ=255)
            aaGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCgaga                                                                                                                                      <  2:1596597/150‑1 (MQ=255)
                ggACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGa                                                                                                                                  >  2:4181184/1‑150 (MQ=255)
                ggACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGa                                                                                                                                  >  1:2103204/1‑150 (MQ=255)
                ggACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGAAGACGCTTTATAGCGAGATTGa                                                                                                                                  >  2:361980/1‑150 (MQ=255)
                 gACAGGAAAAAGACATGACAACTCCGGCGGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGaa                                                                                                                                 >  1:3381774/1‑150 (MQ=255)
                 gACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGaa                                                                                                                                 >  1:2370112/1‑150 (MQ=255)
                  aCAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAg                                                                                                                                >  1:246828/1‑150 (MQ=255)
                      gAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTa                                                                                                                            >  1:4122861/1‑150 (MQ=255)
                      gAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAACCTCGCATTCGACGACCCTTTATAGCGAGATTGAAGCGTa                                                                                                                            >  2:3539769/1‑150 (MQ=255)
                       aaaaaGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGGCGACGCTTTATAGCGAGATTGAAGCGTAt                                                                                                                           >  2:3089399/1‑150 (MQ=255)
                          aaGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTca                                                                                                                        <  1:2514138/150‑1 (MQ=255)
                              cATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACt                                                                                                                    >  1:2491176/1‑150 (MQ=255)
                              cATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCCCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACt                                                                                                                    <  2:1331586/150‑1 (MQ=255)
                                  aCAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAg                                                                                                                <  2:1705382/150‑1 (MQ=255)
                                    aaCTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGAt                                                                                                              >  2:1652446/1‑150 (MQ=255)
                                      cTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCa                                                                                                            <  1:1249354/150‑1 (MQ=255)
                                       tCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAg                                                                                                           >  1:198780/1‑150 (MQ=255)
                                          ggCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTgg                                                                                                        >  2:1509195/1‑150 (MQ=255)
                                            cAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGAt                                                                                                      >  1:1737565/1‑150 (MQ=255)
                                              ggATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTc                                                                                                    >  2:2642068/1‑150 (MQ=255)
                                              ggATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTc                                                                                                    <  2:695429/150‑1 (MQ=255)
                                                aTACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAACGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGa                                                                                                  <  1:3684454/150‑1 (MQ=255)
                                                 tACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGAt                                                                                                 <  1:4066571/150‑1 (MQ=255)
                                                  aCTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATc                                                                                                <  1:1010964/150‑1 (MQ=255)
                                                   cTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCa                                                                                               <  1:182795/150‑1 (MQ=255)
                                                       aGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATa                                                                                           <  2:3059335/150‑1 (MQ=255)
                                                         gCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGc                                                                                         <  1:3829095/150‑1 (MQ=255)
                                                          ccGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCt                                                                                        <  2:2288168/150‑1 (MQ=255)
                                                          ccGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCCACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCt                                                                                        >  1:3109794/1‑150 (MQ=255)
                                                            gCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGt                                                                                      >  1:2888984/1‑150 (MQ=255)
                                                             cAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGtt                                                                                     >  1:56057/1‑150 (MQ=255)
                                                              aaGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTa                                                                                    <  1:3182665/150‑1 (MQ=255)
                                                               aGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTAc                                                                                   <  1:1310992/150‑1 (MQ=255)
                                                                 tgtAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGt                                                                                 >  2:186176/1‑150 (MQ=255)
                                                                   tAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTa                                                                               >  2:2987669/1‑150 (MQ=255)
                                                                    aaCGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTaa                                                                              >  2:1211971/1‑150 (MQ=255)
                                                                    aaCGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTaa                                                                              <  1:804321/150‑1 (MQ=255)
                                                                      cgcgAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACa                                                                            >  1:3513100/1‑150 (MQ=255)
                                                                         gAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCt                                                                         >  1:2178145/1‑150 (MQ=255)
                                                                         gAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCt                                                                         >  2:4269012/1‑150 (MQ=255)
                                                                          aaTCACGCGGCGGGCAAATGTCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTg                                                                        <  2:206215/150‑1 (MQ=255)
                                                                          aaTCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTg                                                                        <  1:109254/150‑1 (MQ=255)
                                                                           aTCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGc                                                                       <  2:3248838/150‑1 (MQ=255)
                                                                            tCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGcc                                                                      >  2:3642935/1‑150 (MQ=255)
                                                                            tCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGcc                                                                      >  1:1445793/1‑150 (MQ=255)
                                                                             cACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCg                                                                     <  2:3824178/150‑1 (MQ=255)
                                                                               cgcgGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGa                                                                   <  2:1260820/150‑1 (MQ=255)
                                                                                gcggcgGGCAAATATCCGAACGCCAGGAGGGAAGATAAAACATAGTAAAAAGCCTCGCCTTCGACGACGCTTTTTAGCGAGATTGAAGCGTATTCACACTTCAGATCAATGGATTCGATCAAAAAGCTGTTACGTTAACAACTGCCGGAc                                                                  >  2:2839939/1‑150 (MQ=255)
                                                                                    cggGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCttt                                                              <  1:2665117/150‑1 (MQ=255)
                                                                                     gggCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTc                                                             <  2:2567747/150‑1 (MQ=255)
                                                                                      ggCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCt                                                            <  1:1154969/150‑1 (MQ=255)
                                                                                           atatCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCg                                                       <  1:3442339/150‑1 (MQ=255)
                                                                                           atatCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGACCg                                                       >  2:3306377/1‑150 (MQ=255)
                                                                                             atCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTc                                                     >  1:2794415/1‑150 (MQ=255)
                                                                                             atCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTc                                                     <  2:3137354/150‑1 (MQ=255)
                                                                                               ccGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCt                                                   <  1:466281/150‑1 (MQ=255)
                                                                                                  aaCGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGaa                                                <  1:1569941/150‑1 (MQ=255)
                                                                                                   aCGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAAt                                               <  1:2297527/150‑1 (MQ=255)
                                                                                                     gCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAAttt                                             >  1:728209/1‑150 (MQ=255)
                                                                                                       cAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACTACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCg                                           <  2:317863/150‑1 (MQ=255)
                                                                                                       cAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCg                                           >  1:276496/1‑150 (MQ=255)
                                                                                                       cAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCg                                           <  2:1786590/150‑1 (MQ=255)
                                                                                                       cAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCg                                           >  2:794751/1‑150 (MQ=255)
                                                                                                        aGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGa                                          <  1:857032/150‑1 (MQ=255)
                                                                                                        aGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGa                                          >  2:589289/1‑150 (MQ=255)
                                                                                                        aGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCCGCCGGACCTTTCTGGCCGTCCTGAATTTCGa                                          >  2:514787/1‑150 (MQ=255)
                                                                                                         ggAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGaa                                         <  2:3485823/150‑1 (MQ=255)
                                                                                                          gAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTTCCGGACCTTTCTGGCCGTCCTGAATTTCGAAc                                        >  1:414681/1‑150 (MQ=255)
                                                                                                               aaGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAAc                                   >  1:4008576/1‑150 (MQ=255)
                                                                                                               aaGATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAAc                                   >  2:1402978/1‑150 (MQ=255)
                                                                                                                 gATAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGt                                 <  1:2964642/150‑1 (MQ=255)
                                                                                                                  aTAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGtt                                >  2:145544/1‑150 (MQ=255)
                                                                                                                   tAAAGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTc                               >  2:3972454/1‑150 (MQ=255)
                                                                                                                    aaaGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCt                              >  2:2717689/1‑150 (MQ=255)
                                                                                                                    aaaGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCt                              >  2:1301878/1‑150 (MQ=255)
                                                                                                                     aaGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTg                             >  2:1914439/1‑150 (MQ=255)
                                                                                                                      aGCATAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGa                            <  1:594126/150‑1 (MQ=255)
                                                                                                                         aTAGTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCt                         >  1:1661026/1‑150 (MQ=255)
                                                                                                                            gTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCa                      <  2:3998783/150‑1 (MQ=255)
                                                                                                                            gTAAAAAGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCa                      >  1:331744/1‑150 (MQ=255)
                                                                                                                              aaaaaGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGc                    >  2:3700831/1‑150 (MQ=255)
                                                                                                                                aaaGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCa                  >  1:3802000/1‑150 (MQ=255)
                                                                                                                                 aaGCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCag                 >  1:3918946/1‑150 (MQ=255)
                                                                                                                                   gCCTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCagag               >  2:952548/1‑150 (MQ=255)
                                                                                                                                    ccTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGt              >  1:1023933/1‑150 (MQ=255)
                                                                                                                                     cTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGtt             >  2:2906437/1‑150 (MQ=255)
                                                                                                                                     cTCGCATTCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGtt             >  1:1913578/1‑150 (MQ=255)
                                                                                                                                           ttCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAg       >  1:672216/1‑150 (MQ=255)
                                                                                                                                           ttCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAg       <  1:4273284/150‑1 (MQ=255)
                                                                                                                                            tCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc      <  2:2430056/150‑1 (MQ=255)
                                                                                                                                            tCGACGACGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc      >  2:612861/1‑150 (MQ=255)
                                                                                                                                              gacgacGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCa    >  2:2347454/1‑150 (MQ=255)
                                                                                                                                               acgacgCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCAt   >  2:2131043/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGGCTGATGGCAAAGTGGACAGGAAAAAGACATGACAACTCCGGCAGGATACTAAAGGCCGCAAGTGTAACGCGAATCACGCGGCGGGCAAATATCCGAACGCCAGGAGCGAAGATAAAGCATAGTAAAAAGCCTCGCATTCGACGAGGCTTTATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATT  >  AP012306/1587174‑1587467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑