BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	2,412,842	A→C	K138Q (AAA→CAA)	ygcE →	predicted kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	2,412,842	0	A	C	100.0%	1135.8 / NA	271	K138Q (AAA→CAA)	ygcE	predicted kinase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (139/132); total (139/132)

Only 100 of 278 total aligned reads displayed.

TATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTAAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACAT  >  AP012306/2412696‑2412988

  ttGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGAc                                                                                                                                               <  1:2717820/150‑1 (MQ=255)

  ttGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGAc                                                                                                                                               <  2:3772527/150‑1 (MQ=255)

   tGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACa                                                                                                                                              <  1:3431179/150‑1 (MQ=255)

   tGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACa                                                                                                                                              >  1:3310826/1‑150 (MQ=255)

    gAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGC‑TAACCGGCGAGTTTCAAGACAat                                                                                                                                            <  2:3199904/150‑1 (MQ=255)

      aaGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAata                                                                                                                                           >  1:4076701/1‑150 (MQ=255)

            aTGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCa                                                                                                                                     >  1:1274261/1‑150 (MQ=255)

             tGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCaa                                                                                                                                    >  2:1012492/1‑150 (MQ=255)

              ggCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAAc                                                                                                                                   <  1:2026239/150‑1 (MQ=255)

                   gCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTAttt                                                                                                                              >  2:881313/1‑150 (MQ=255)

                   gCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTAttt                                                                                                                              <  2:4176016/150‑1 (MQ=255)

                   gCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTAttt                                                                                                                              >  2:521368/1‑150 (MQ=255)

                    ccTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTAtttt                                                                                                                             >  2:734887/1‑150 (MQ=255)

                    ccTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTAtttt                                                                                                                             >  2:2519096/1‑150 (MQ=255)

                    ccTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTAtttt                                                                                                                             >  2:2393724/1‑150 (MQ=255)

                      tgctgcGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTgg                                                                                                                           <  1:2084796/150‑1 (MQ=255)

                       gctgcGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGt                                                                                                                          <  1:3191376/150‑1 (MQ=255)

                        ctgcGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTc                                                                                                                         <  1:2945552/150‑1 (MQ=255)

                            gcCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTg                                                                                                                     <  1:2691889/150‑1 (MQ=255)

                                 tGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCgg                                                                                                                >  2:692986/1‑150 (MQ=255)

                                  gATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGt                                                                                                               >  1:1696310/1‑150 (MQ=255)

                                  gATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGt                                                                                                               >  2:1438834/1‑150 (MQ=255)

                                  gATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGt                                                                                                               <  2:2327621/150‑1 (MQ=255)

                                      aGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGAt                                                                                                           >  1:735841/1‑150 (MQ=255)

                                      aGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGAt                                                                                                           >  1:718083/1‑150 (MQ=255)

                                       gCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGAtt                                                                                                          >  1:3036157/1‑150 (MQ=255)

                                        cTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATta                                                                                                         <  2:2931951/150‑1 (MQ=255)

                                         tGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATtat                                                                                                        <  1:2667614/150‑1 (MQ=255)

                                         tGGCAGGATGCACGCGTTACACGCCCTTACGAACACACGAACCCTGACGTGGCATATGTCGCCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTAAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATtat                                                                                                        <  2:4223663/150‑1 (MQ=255)

                                           gCAGGATGCACGCGTTACACGCCCTTACGAACCCCCTACCCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATaa                                                                                                      <  2:4054088/150‑1 (MQ=255)

                                           gCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATaa                                                                                                      >  2:4069974/1‑150 (MQ=255)

                                           gCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATaa                                                                                                      <  2:1130007/150‑1 (MQ=255)

                                             aGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAaga                                                                                                    >  1:595538/1‑150 (MQ=255)

                                             aGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAaga                                                                                                    >  1:1268503/1‑150 (MQ=255)

                                                aTGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCt                                                                                                 <  2:2552750/150‑1 (MQ=255)

                                                  gCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTgg                                                                                               <  1:630352/150‑1 (MQ=255)

                                                  gCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTgg                                                                                               >  2:2784908/1‑150 (MQ=255)

                                                    aCGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGc                                                                                             >  1:2209802/1‑150 (MQ=255)

                                                     cgcgTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCa                                                                                            >  2:1659280/1‑150 (MQ=255)

                                                      gcgTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCAt                                                                                           <  1:515517/150‑1 (MQ=255)

                                                         ttACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGa                                                                                        <  2:3101581/150‑1 (MQ=255)

                                                         ttACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGa                                                                                        >  1:1637884/1‑150 (MQ=255)

                                                          tACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAg                                                                                       <  2:1569426/150‑1 (MQ=255)

                                                           acacGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGc                                                                                      >  2:3367529/1‑150 (MQ=255)

                                                           acacGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGc                                                                                      <  2:2570892/150‑1 (MQ=255)

                                                           acacGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGc                                                                                      <  2:1494005/150‑1 (MQ=255)

                                                           acacGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGc                                                                                      >  1:2750591/1‑150 (MQ=255)

                                                                  cTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGAtg                                                                               <  1:2147670/150‑1 (MQ=255)

                                                                      cGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGAtgctgc                                                                           >  1:133154/1‑150 (MQ=255)

                                                                       gAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCg                                                                          >  1:3451029/1‑150 (MQ=255)

                                                                        aaCACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCgg                                                                         <  1:244584/150‑1 (MQ=255)

                                                                        aaCACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCgg                                                                         >  2:3154477/1‑150 (MQ=255)

                                                                         acacacTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGt                                                                        <  2:3133447/150‑1 (MQ=255)

                                                                         acacacTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGt                                                                        >  1:1113557/1‑150 (MQ=255)

                                                                            cacTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAAt                                                                     <  2:3839909/150‑1 (MQ=255)

                                                                             acTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATg                                                                    >  2:2995947/1‑150 (MQ=255)

                                                                              cTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTTGGCATGGAGCGAAGATGCTGCGGTAATgg                                                                   >  2:4234347/1‑150 (MQ=255)

                                                                              cTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATgg                                                                   <  1:3814765/150‑1 (MQ=255)

                                                                                aaCCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGAt                                                                 >  2:1261320/1‑150 (MQ=255)

                                                                                 aCCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATa                                                                <  1:2010601/150‑1 (MQ=255)

                                                                                      gACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGttt                                                           <  2:1759496/150‑1 (MQ=255)

                                                                                      gACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGttt                                                           <  1:1021630/150‑1 (MQ=255)

                                                                                        cGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTaa                                                         >  2:2162499/1‑150 (MQ=255)

                                                                                           ggCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAatat                                                      >  2:223902/1‑150 (MQ=255)

                                                                                             cATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATcc                                                    <  2:815960/150‑1 (MQ=255)

                                                                                             cATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATcc                                                    >  2:1305982/1‑150 (MQ=255)

                                                                                               tatGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATcccc                                                  <  1:3826349/150‑1 (MQ=255)

                                                                                                  gTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGt                                               >  2:146653/1‑150 (MQ=255)

                                                                                                    cACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCa                                             >  1:336451/1‑150 (MQ=255)

                                                                                                       ctctTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCatat                                          >  1:3237321/1‑150 (MQ=255)

                                                                                                       ctctTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCatat                                          <  2:4104317/150‑1 (MQ=255)

                                                                                                        tctTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATg                                         >  1:3964209/1‑150 (MQ=255)

                                                                                                         ctTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGc                                        >  2:462249/1‑150 (MQ=255)

                                                                                                         ctTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGc                                        >  1:827577/1‑150 (MQ=255)

                                                                                                          tttttCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCt                                       <  2:1975084/150‑1 (MQ=255)

                                                                                                            tttCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGt                                     <  1:2326761/150‑1 (MQ=255)

                                                                                                             ttCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGtt                                    >  1:3588975/1‑150 (MQ=255)

                                                                                                              tCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGttt                                   <  1:3748829/150‑1 (MQ=255)

                                                                                                                gggTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGa                                 >  2:30191/1‑150 (MQ=255)

                                                                                                                  gTTATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGAtg                               <  1:1158210/150‑1 (MQ=255)

                                                                                                                    tATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGAtgtg                             >  1:1361474/1‑150 (MQ=255)

                                                                                                                    tATCTGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGAtgtg                             <  2:3723250/150‑1 (MQ=255)

                                                                                                                        tGACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCaaa                         >  2:2839457/1‑150 (MQ=255)

                                                                                                                         gACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAAt                        <  2:2635856/150‑1 (MQ=255)

                                                                                                                         gACGCATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAAt                        <  1:4186417/150‑1 (MQ=255)

                                                                                                                             cATCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCt                    <  1:4194405/150‑1 (MQ=255)

                                                                                                                               tCGCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTgg                  >  1:1061170/1‑150 (MQ=255)

                                                                                                                                 gCTTAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCa                >  2:636/1‑150 (MQ=255)

                                                                                                                                   ttAACCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCAcc              <  2:2067452/150‑1 (MQ=255)

                                                                                                                                      aCCGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTc           <  2:3622447/150‑1 (MQ=255)

                                                                                                                                       ccGGCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTcc          <  2:1355576/150‑1 (MQ=255)

                                                                                                                                         ggCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTc        >  1:1722146/1‑150 (MQ=255)

                                                                                                                                         ggCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTc        >  2:553332/1‑150 (MQ=255)

                                                                                                                                         ggCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTc        <  2:681981/150‑1 (MQ=255)

                                                                                                                                          gCGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCg       >  1:663447/1‑150 (MQ=255)

                                                                                                                                           cGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATTCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCgg      >  1:1777255/1‑150 (MQ=255)

                                                                                                                                           cGAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCgg      >  1:2404048/1‑150 (MQ=255)

                                                                                                                                            gAGTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGa     <  1:3690644/150‑1 (MQ=255)

                                                                                                                                              gTTTCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACa   <  1:2834801/150‑1 (MQ=255)

                                                                                                                                               tttCAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACat  <  2:1866899/150‑1 (MQ=255)

TATTGAAAGCCGATGGCACGCCTGCTGCGCCGTTGATTAGCTGGCAGGATGCACGCGTTACACGCCCTTACGAACACACTAACCCTGACGTGGCATATGTCACCTCTTTTTCGGGTTATCTGACGCATCGCTTAACCGGCGAGTTTAAAGACAATATCGCCAACTATTTTGGTCAGTGGCCGGTGGATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACAT  >  AP012306/2412696‑2412988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`