Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306202,4170TG27.3% 624.6 / 27.6 273V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base T (77/120);  new base G (74/0);  total (153/120)
Fisher's exact test for biased strand distribution p-value = 3.58e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 324 total aligned reads displayed.

CAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACCG  >  AP012306/202271‑202566
                                                                                                                                                  |                                                                                                                                                     
     cgcCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGGCGGGGTATTTGTGa                                                                                                                                               >  1:165783/1‑150 (MQ=255)
       ccAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACg                                                                                                                                             <  2:801160/150‑1 (MQ=255)
       ccAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTTTGACg                                                                                                                                             >  1:2017639/1‑150 (MQ=255)
            aTTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCaaga                                                                                                                                        <  1:1246112/150‑1 (MQ=255)
             ttGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCaagaa                                                                                                                                       >  1:3592021/1‑150 (MQ=255)
              tgctgcATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCaagaag                                                                                                                                      >  1:1870467/1‑150 (MQ=255)
               gctgcATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAgg                                                                                                                                     >  1:3493519/1‑150 (MQ=255)
                ctgcATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTTTTGCGGAAGAAggg                                                                                                                                    >  2:648137/1‑149 (MQ=255)
                ctgcATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGGGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGGTTGCGGCGGCCGGGGGATTTGTGAAGCAAGAAggg                                                                                                                                    >  2:2887035/1‑149 (MQ=255)
                 tgcATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGgtg                                                                                                                                   <  1:4402099/150‑1 (MQ=255)
                 tgcATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGgtg                                                                                                                                   <  1:2951335/150‑1 (MQ=255)
                  gcATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTTTGACGCAAGAAGgtgt                                                                                                                                  >  1:3672287/1‑150 (MQ=255)
                   cATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGGCGGGGGATTTTTGGCGCAAGAAGGggtg                                                                                                                                 >  2:3576824/1‑150 (MQ=255)
                   cATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCCGGGGGATTTTTGGCGCaagaaaggggg                                                                                                                                 >  2:1589482/1‑144 (MQ=255)
                     tatTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGc                                                                                                                               <  2:4291941/150‑1 (MQ=255)
                       ttGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTg                                                                                                                             <  1:2156332/150‑1 (MQ=255)
                        tGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGGTGGGCGGGCCGCCCGGGCTACGTGACACCACGCCCCTGCTGTTTTATGGCGAAACGGTGATTGCGGCGGCCGGGGGATTTGTTTCCGAAGAAGGGGTGGCGGa                                                                                                                            >  2:2521185/1‑150 (MQ=255)
                         gTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCCGGGGGATTTGTGACGCAAGAAGGTGTGGCTGaa                                                                                                                           >  1:1436448/1‑150 (MQ=255)
                         gTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGaa                                                                                                                           >  1:2652156/1‑150 (MQ=255)
                           cGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAgg                                                                                                                         <  2:1144608/150‑1 (MQ=255)
                           cGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAgg                                                                                                                         >  1:2674754/1‑150 (MQ=255)
                           cGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGGGTGGCTGGAgg                                                                                                                         >  1:3115150/1‑150 (MQ=255)
                            gggCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTTTGACGCAAGAAGGGGTGGGTGGAggg                                                                                                                        >  2:3764270/1‑149 (MQ=255)
                               cGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTTTGACGCAAGAAGGTGTGGCTGAAgggggg                                                                                                                     >  2:1566384/1‑146 (MQ=255)
                                gTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTgaga                                                                                                                    <  2:4216709/150‑1 (MQ=255)
                                gTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTgaga                                                                                                                    <  1:2284320/150‑1 (MQ=255)
                                gTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTTTGACGCAAGGAGGTGTGGCTGGAgggggga                                                                                                                    >  1:1225001/1‑145 (MQ=255)
                                 tAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGGCGGGGGATTTGTGGCGCAAGAAAGGGTGGCTGGAGGGGAGaa                                                                                                                   >  2:2575727/1‑150 (MQ=255)
                                 tAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTTTGACGCAAGAAGGGGGGGGTGAAGGGGAGaa                                                                                                                   >  2:3701408/1‑150 (MQ=255)
                                  aaCGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAAt                                                                                                                  <  1:2112272/150‑1 (MQ=255)
                                     ggcggACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGc                                                                                                               >  1:1093407/1‑150 (MQ=255)
                                         gACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTaa                                                                                                           >  1:4331558/1‑150 (MQ=255)
                                         gACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTaa                                                                                                           >  1:2414619/1‑150 (MQ=255)
                                         gACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTaa                                                                                                           >  2:2453426/1‑150 (MQ=255)
                                          aCGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGGATGGCGTAAg                                                                                                          >  2:2988922/1‑150 (MQ=255)
                                            gTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTTTGACGCAAGAAGGTGTGGCTGGAGGGGACAATGGCGTAAGtt                                                                                                        >  2:3434823/1‑150 (MQ=255)
                                            gTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGtt                                                                                                        <  1:4279341/150‑1 (MQ=255)
                                             tAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGGGTGGCTGAAGGTGAGAAAGGCGGAAGgtt                                                                                                       >  2:3161205/1‑150 (MQ=255)
                                               aGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGGTTTg                                                                                                     >  1:259981/1‑150 (MQ=255)
                                               aGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGGGGGGCTGAAGGGGAGAATGGCGGAAGTTTTg                                                                                                     >  2:3655219/1‑150 (MQ=255)
                                                gCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGt                                                                                                    >  2:1938388/1‑150 (MQ=255)
                                                 cTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTc                                                                                                   >  1:3180532/1‑150 (MQ=255)
                                                 cTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTc                                                                                                   >  1:2516493/1‑150 (MQ=255)
                                                   aaaGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTg                                                                                                 >  1:3222103/1‑150 (MQ=255)
                                                   aaaGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAAAGGCGTAAGTTTTGTCTg                                                                                                 >  2:1643587/1‑150 (MQ=255)
                                                    aaGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGGGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTgg                                                                                                >  2:3900739/1‑150 (MQ=255)
                                                      gAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCa                                                                                              >  2:440037/1‑150 (MQ=255)
                                                         aaTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGaa                                                                                           <  2:3126702/150‑1 (MQ=255)
                                                          aTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGaaa                                                                                          <  1:4192020/150‑1 (MQ=255)
                                                          aTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGaaa                                                                                          >  1:484213/1‑150 (MQ=255)
                                                             tGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACg                                                                                       <  2:618785/150‑1 (MQ=255)
                                                                   gagCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGGGTGGCTGAAGGGGAGAATGGCGTAAGGTTTGTCTGGCAGAAAACGCTTAGt                                                                                 >  2:2592812/1‑150 (MQ=255)
                                                                       tGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAg                                                                             <  1:3943396/150‑1 (MQ=255)
                                                                          gCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGa                                                                          <  2:1516973/150‑1 (MQ=255)
                                                                            gTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGaaa                                                                        >  1:68237/1‑150 (MQ=255)
                                                                             tGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAg                                                                       >  1:2722705/1‑150 (MQ=255)
                                                                                cgccgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCg                                                                    <  2:1013335/150‑1 (MQ=255)
                                                                                  ccgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGa                                                                  <  1:1964277/150‑1 (MQ=255)
                                                                                  ccgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGa                                                                  <  2:1430498/150‑1 (MQ=255)
                                                                                   cgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGAt                                                                 <  1:784122/150‑1 (MQ=255)
                                                                                   cgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAATGAAAGCCGGAt                                                                 >  2:557907/1‑150 (MQ=255)
                                                                                     tGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATaa                                                               >  1:4075603/1‑150 (MQ=255)
                                                                                      ggCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAg                                                              <  2:2437592/150‑1 (MQ=255)
                                                                                       gCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGa                                                             >  1:1624335/1‑150 (MQ=255)
                                                                                       gCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGGGTGGCTGAAGGTGAGAATGGCGTAAGTTTTTTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGa                                                             >  2:921940/1‑150 (MQ=255)
                                                                                        cTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGGCGCAAGAAGGTGTGGCTGGAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAAAGGTTTGTTAAATGAAAGCCGGGTaaaaa                                                            >  2:1698223/1‑147 (MQ=255)
                                                                                             tGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATc                                                       <  1:3300953/150‑1 (MQ=255)
                                                                                              gACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGGAGGGGGATTTGTGACGCAAGAAGGGGGGGGTGAAGGGGAGAATGGGGGAAGGTTTGTCTGGGAGAAAAAGGTTAGTTAAGGGAAAGCCGGATAAGAGGGATCa                                                      >  2:3854098/1‑150 (MQ=255)
                                                                                              gACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCa                                                      >  1:579896/1‑150 (MQ=255)
                                                                                                   cacGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGt                                                 <  1:810942/150‑1 (MQ=255)
                                                                                                     cGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCg                                               >  1:2225964/1‑150 (MQ=255)
                                                                                                     cGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCg                                               >  1:234838/1‑150 (MQ=255)
                                                                                                     cGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAACCGGATAAGACGCATCAAACGTCg                                               >  1:178911/1‑150 (MQ=255)
                                                                                                       ccACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGGTAAGACGCATCAAACGTCGCa                                             >  1:2483295/1‑150 (MQ=255)
                                                                                                       ccACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCa                                             >  1:3068155/1‑150 (MQ=255)
                                                                                                        cACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCAt                                            <  1:3858427/150‑1 (MQ=255)
                                                                                                        cACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCAt                                            <  1:2049829/150‑1 (MQ=255)
                                                                                                        cACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCAt                                            <  2:3327663/150‑1 (MQ=255)
                                                                                                        cACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGGTAAGACGCATCAAACGTCGCAt                                            >  2:3549521/1‑150 (MQ=255)
                                                                                                          ctgctgTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATcc                                          <  2:2799193/150‑1 (MQ=255)
                                                                                                          ctgctgTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATcc                                          <  2:2019178/150‑1 (MQ=255)
                                                                                                                  ttATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAg                                  <  1:1341558/150‑1 (MQ=255)
                                                                                                                   tATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGGGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCCGCGAAAGt                                 >  1:3246263/1‑150 (MQ=255)
                                                                                                                    aTGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTc                                >  1:929950/1‑150 (MQ=255)
                                                                                                                    aTGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGGGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTc                                >  2:2689935/1‑150 (MQ=255)
                                                                                                                        cGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATc                            <  2:1370923/150‑1 (MQ=255)
                                                                                                                        cGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGGGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATc                            >  2:4119258/1‑150 (MQ=255)
                                                                                                                         gAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCa                           >  1:377885/1‑150 (MQ=255)
                                                                                                                         gAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCa                           >  1:1708605/1‑150 (MQ=255)
                                                                                                                          aaaCGCTGATTGCGGCGGCAGGGGCAGTTGTGGCGCAAGAAGGGGTGGCTGAAGGGGGGAATGGCGTAAGTTTTGTCTGGCAGAAAAAGCTTCGTTAAGTGAAAACCGGATAAAACCCATCAAACGTCCCCTCCGGCGTAAGGCAAACAg                          >  2:222851/1‑150 (MQ=255)
                                                                                                                           aaCGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAACCAgg                         <  1:977838/150‑1 (MQ=255)
                                                                                                                           aaCGCTGATTGCGGCGGCAGGGGAATTTGTGACGCAAGAAGGAGTGGCTGAAGGTGAGAATGGCGTAAGATTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCACATCCGGCGAAAGTCAAACAgg                         >  2:2397377/1‑150 (MQ=255)
                                                                                                                               cTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTc                     <  2:1881929/150‑1 (MQ=255)
                                                                                                                                 gATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGc                   >  2:4008230/1‑150 (MQ=255)
                                                                                                                                         cggcAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTaccacc           >  1:2814105/1‑150 (MQ=255)
                                                                                                                                         cggcAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTaccacc           >  2:812799/1‑150 (MQ=255)
                                                                                                                                          ggcAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTaccacca          >  1:302063/1‑150 (MQ=255)
                                                                                                                                              ggggTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGCCAATCAGGATTCGCTTACCACCACCGt      >  2:476982/1‑150 (MQ=255)
                                                                                                                                               gggTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTa     >  1:4001775/1‑150 (MQ=255)
                                                                                                                                                 gTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTAcc   <  1:4371139/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACCG  >  AP012306/202271‑202566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑