Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306935,0540GT28.9% 179.5 / 45.5 76intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
Reads supporting (aligned to +/- strand):  ref base G (10/44);  new base T (22/0);  total (32/44)
Fisher's exact test for biased strand distribution p-value = 8.88e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 148 total aligned reads displayed.

GGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGCCTCGCCATAT‑AAATTGAAT‑GGTGC‑AGGGAGCGCG‑CAGGGGGC‑GGCCAAT‑CGCCGCCGCCCCCTGCT‑GTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACA  >  AP012306/934905‑935203
                                                                                                                                                       |                                                                                                                                                               
ggCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑Ttttg                                                                                                                                                                 >  1:1878855/1‑150 (MQ=255)
ggCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑Ttttg                                                                                                                                                                 >  2:4264397/1‑150 (MQ=255)
 gCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑Ttttgt                                                                                                                                                                <  2:1910579/150‑1 (MQ=255)
 gCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑Ctttgt                                                                                                                                                                >  2:3490056/1‑150 (MQ=255)
  cAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑Ttttttt                                                                                                                                                               >  1:3642029/1‑150 (MQ=255)
  cAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑Ttttgtt                                                                                                                                                               <  1:994779/150‑1 (MQ=255)
   aGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑Gttttttt                                                                                                                                                              >  1:4426512/1‑150 (MQ=255)
   aGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑Gttttttt                                                                                                                                                              >  2:4189309/1‑150 (MQ=255)
     tATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTgg                                                                                                                                                            <  1:2458146/150‑1 (MQ=255)
      aTTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAAGGACTTAGCCTGCGGC‑GG‑GGTTGTGTggt                                                                                                                                                           >  2:4397900/1‑149 (MQ=255)
         gggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGG‑TTc                                                                                                                                                       >  1:3658044/1‑150 (MQ=255)
         gggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTc                                                                                                                                                       <  1:946989/150‑1 (MQ=255)
         gggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTc                                                                                                                                                       <  1:252988/150‑1 (MQ=255)
         gggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTc                                                                                                                                                       <  1:2372612/150‑1 (MQ=255)
         gggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTGTTTTGG‑TTc                                                                                                                                                       >  1:412887/1‑150 (MQ=255)
         gggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTGTTTTGC‑GTc                                                                                                                                                       >  1:3635750/1‑150 (MQ=255)
          ggATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGG‑TTct                                                                                                                                                      >  2:2560773/1‑149 (MQ=255)
            aTTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTGTC‑TTcctg                                                                                                                                                    >  1:2316996/1‑147 (MQ=255)
            aTTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTGGC‑TTctgc                                                                                                                                                    >  2:3926334/1‑150 (MQ=255)
            aTTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTGTC‑TTctgc                                                                                                                                                    >  2:2794682/1‑150 (MQ=255)
            aTTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑ATTTTTTTGGG‑TTcagc                                                                                                                                                    >  1:754338/1‑150 (MQ=255)
                tAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATGATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGG‑TTCAGGAGC‑g                                                                                                                                               >  2:3561277/1‑150 (MQ=255)
                 aGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCATGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGC‑TTCAGCAGC‑gg                                                                                                                                              >  1:3195046/1‑150 (MQ=255)
                   tgtTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGG‑TTCAGCAGC‑gggg                                                                                                                                            >  2:4125574/1‑149 (MQ=255)
                     tttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGG‑TTCAGCAGC‑GGGGTg                                                                                                                                          >  1:4246595/1‑150 (MQ=255)
                      ttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTGACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑CTTTTTTTTGG‑GTCAGCGGG‑GGGGTgc                                                                                                                                         >  1:3993027/1‑150 (MQ=255)
                      ttAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTGTTTTGC‑TTCTGCACC‑GGGGTgt                                                                                                                                         >  1:1756763/1‑149 (MQ=255)
                        aGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGG‑TTCAGCAAC‑GGGTTgcg‑c                                                                                                                                      >  1:1806872/1‑150 (MQ=255)
                         gTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGC‑TTCAGCAGC‑GGGGTGCG‑Ct                                                                                                                                     >  1:1206700/1‑150 (MQ=255)
                           ttGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTcc                                                                                                                                   <  2:133833/150‑1 (MQ=255)
                            tGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGG‑TTCCGCAGC‑GGGTTGCG‑CTccc                                                                                                                                  >  1:3807468/1‑150 (MQ=255)
                             gCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGC‑TTCAGCAGC‑CGGGTGCG‑CTcccc                                                                                                                                 >  1:2894786/1‑149 (MQ=255)
                              cTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGCTTTCTGC‑GC‑GGGGTGCG‑CGCCCtt                                                                                                                                >  2:809530/1‑150 (MQ=255)
                               tGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGC‑TTCAGCAGC‑GGGGTGCG‑CTCCCCTa                                                                                                                               >  1:2324987/1‑150 (MQ=255)
                                gggTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGG‑TTTTTTTTGGT‑TTC‑GCAGC‑GGGGTGGG‑CTcccctta                                                                                                                              >  2:3436275/1‑146 (MQ=255)
                                gggTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTaa                                                                                                                              <  1:1027533/150‑1 (MQ=255)
                                 ggTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAAt                                                                                                                             <  1:2900075/150‑1 (MQ=255)
                                  gTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGG‑TTCGCCAAC‑TGGCTGCG‑CGCCCTTTAtg                                                                                                                            >  2:2645271/1‑150 (MQ=255)
                                   tAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAAtgt                                                                                                                           <  1:556372/150‑1 (MQ=255)
                                   tAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAAtgt                                                                                                                           <  2:3349875/150‑1 (MQ=255)
                                   tAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGC‑TTCCGCAGC‑CGGTTCCC‑CTccccttattg                                                                                                                           >  1:3066669/1‑143 (MQ=255)
                                     aaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGc                                                                                                                         <  2:81938/150‑1 (MQ=255)
                                     aaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGc                                                                                                                         <  1:2707615/150‑1 (MQ=255)
                                     aaGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGc                                                                                                                         <  1:2080096/150‑1 (MQ=255)
                                      aGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGcc                                                                                                                        <  1:2071224/150‑1 (MQ=255)
                                          ccGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTTAGCAGC‑GGGGTGCG‑CTCCCCTTATGTGGCTCGc                                                                                                                    >  1:274410/1‑150 (MQ=255)
                                           cGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGC‑CTTCGCAGC‑GGGTTGCG‑CTCCCCTTATTTGGCTCGcc                                                                                                                   >  2:1677439/1‑150 (MQ=255)
                                            gTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGG‑TTCAGCAGC‑GGGGTGGG‑CTCCCCTTATGTGCCCCGccc                                                                                                                  >  2:3539808/1‑149 (MQ=255)
                                            gTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGCCTCGCCa                                                                                                                  <  2:3992351/150‑1 (MQ=255)
                                            gTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGCCTCGCCa                                                                                                                  <  1:2332481/150‑1 (MQ=255)
                                            gTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGC‑TTCAACCGC‑GGGGTGCG‑CCCCCCTTATGTTcccccgcc                                                                                                                  >  1:1768423/1‑144 (MQ=255)
                                              cAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGCCTCGCCata                                                                                                                <  1:1657091/150‑1 (MQ=255)
                                                gcggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTTATGTGCCTCGCCCtat‑a                                                                                                             >  1:193721/1‑150 (MQ=255)
                                                 cggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGC‑TTCAGCAGC‑GGGGTTCG‑CTCCCTTAATGTGCCTCGCCCTAT‑aa                                                                                                            >  1:1383331/1‑150 (MQ=255)
                                                  ggcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGCCTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGCCTCGCCATAT‑aaa                                                                                                           <  2:1124253/150‑1 (MQ=255)
                                                   gcgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGG‑TTTTGCAGC‑GGGGTGGG‑GTCCCCTTATGTGCCTCGCCCTTT‑AAAt                                                                                                          >  2:2891516/1‑150 (MQ=255)
                                                    cgATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGG‑TTCAGCAGC‑GGGGTGCG‑GCCCCCTTATATGCCTCCCCCTAT‑AAAtt                                                                                                         >  1:1951496/1‑150 (MQ=255)
                                                     gATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGC‑TTCAGCAGC‑GGGGTGCG‑CTCCCCTTATGTGCCTCGCCCTAT‑AAATTg                                                                                                        >  1:676842/1‑150 (MQ=255)
                                                      aTGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTGTTGGC‑CTTCGCACC‑GGGGTTCG‑CTCCCCTTATGTGCCTCCCCCTCT‑aaaattg                                                                                                       >  1:251671/1‑146 (MQ=255)
                                                      aTGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGC‑TTCTGCAGC‑GGGGTGCG‑CCCCCCTAATGTTCCTTCCCACAT‑TAAtttg                                                                                                       >  1:841331/1‑148 (MQ=255)
                                                       tGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGCCTCGCCATAT‑AAATTGaa                                                                                                      <  1:3723356/150‑1 (MQ=255)
                                                        gCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGCCTCGCCATAT‑AAATTGAAt                                                                                                     <  2:1746469/150‑1 (MQ=255)
                                                         cAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGCCTCGCCATAT‑AAATTGAAT‑g                                                                                                   <  1:2859317/150‑1 (MQ=255)
                                                          aaaaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGG‑TTTCGCCGC‑GGGGTTGG‑GTCCCTTTATTTGGCTCGCCCTAT‑AAATTTAAT‑gg                                                                                                  >  2:2333589/1‑150 (MQ=255)
                                                          aaaaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGCCTCGCCATAT‑AAATTGAAT‑gg                                                                                                  <  2:3449716/150‑1 (MQ=255)
                                                            aaaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTTGC‑TTTAGCAGC‑GGGGTGCG‑CTCCCTTAATGTTCCTCGCCCTAT‑AAATTTAAT‑GGTg                                                                                                >  1:4076869/1‑150 (MQ=255)
                                                             aaCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGCCTCGCCATAT‑AAATTGAAT‑GGTGc                                                                                               <  1:3242729/150‑1 (MQ=255)
                                                              aCGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGCCTCGCCATAT‑AAATTGAAT‑GGTGC‑a                                                                                             <  2:430374/150‑1 (MQ=255)
                                                                    tGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGCTTTTTTTTGGC‑‑TCAGCCGC‑GGGGTGGG‑CTCCCCTTATTTGGCTCGCCCCAT‑AAAATTAATAGGGGC‑Aggggg                                                                                        >  2:4348736/1‑148 (MQ=255)
                                                                       cGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGG‑TTCTGCAGC‑GGGGTTGG‑CTCCCCTTATGTTGCTCGCCCTAT‑AAATTTTAT‑GGTGC‑AGGGAgggcg                                                                                    >  2:3988354/1‑150 (MQ=255)
                                                                         aGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGCCTCGCCATAT‑AAATTGAAT‑GGTGC‑AGGGAGCGCG‑Ca                                                                                 <  1:1522462/150‑1 (MQ=255)
                                                                             cGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTGGG‑TTTAGCAGC‑GGGGTGCG‑CTCCCTTAATGTGCCCCGCCCTAT‑AAATTTAAT‑TGTGG‑AGGGGGCGCG‑CCgggg                                                                             >  2:4084763/1‑150 (MQ=255)
                                                                             cGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGCCTCGCCATAT‑AAATTGAAT‑GGTGC‑AGGGAGCGCG‑CAgggg                                                                             <  1:509217/150‑1 (MQ=255)
                                                                                  cgcACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GGTTTTTGTTTGGT‑TTGA‑CAGC‑GGGGTGGG‑CGCCCCTTATTTGGCCCGCCCTATAAAATTGAAT‑‑GTGC‑AGGGGGGGCG‑CAgggggg‑ggg                                                                       >  2:3853514/1‑149 (MQ=255)
                                                                                        aTCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGC‑TTCCGCAGC‑GGGTGGTG‑CCCCCTTAATGTGCCTCCCCCTAT‑AAATTTGAA‑GGGGG‑GGGGGGGGCG‑CAGGGGGG‑GGCCAAT‑cg                                                                >  2:1321220/1‑150 (MQ=255)
                                                                                               ggCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGC‑CTTAGCCGC‑GCGGTGTG‑GCCCCTTTATTTGCCTCGCCGCAT‑AAAATGAAA‑GGGGC‑AGGGGGCGCG‑CACGGGGG‑GGCCCAT‑cgcccccgc                                                         >  2:3461859/1‑150 (MQ=255)
                                                                                                      ccgTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTTGG‑TTTAACAGC‑GGGGTGGGCCCCCCTTAAT‑TGCCTCGCCCCAT‑AAATTTAAT‑GGGGC‑GGGGGGCGCG‑CCGGGGGCGGGCCAAc‑ccccccccccccccc                                                   >  2:2459193/1‑148 (MQ=255)
                                                                                                        gTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTGGG‑TTGAGGAGG‑GGGGGGCG‑GGCCCTTTAAGTGGCGGGCCCTATAAAATTAAAA‑GGGAG‑AGGG‑GGGGG‑CAGGGGGG‑GGGGAAG‑CGCCTCCGCCCCccgcct                                                >  2:1469291/1‑148 (MQ=255)
                                                                                                              gTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTGGC‑TTCAGCAGC‑GGGGTGGG‑CTCCCCTTATGTGCCTCCCCCTAT‑AAATTTAAT‑GGGGG‑CGGGGGGGCG‑CAGGGGGG‑GGCCAAA‑CGCCCCCCCCCCCCCCT‑GTccccg                                         >  2:2661888/1‑148 (MQ=255)
                                                                                                               ttGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTGGG‑TTCCGCAAC‑GGGGGGCG‑CTCCCCTAATGTGCCTCGCCATAT‑AAAATGAAA‑GGTGG‑AGGGGGCGCG‑CAGGGGGG‑GGGCCAT‑CTCCGCCCCCCCCCCCC‑GTCCCCGc                                        >  2:3484215/1‑150 (MQ=255)
                                                                                                               ttGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑GTTTTTTTGGC‑TTTAGCACC‑GGGGTGGG‑CTCCCCTAATGTGCGTCGGCAAAA‑AAATTGAAT‑GGTGG‑GGGGGGGGGG‑CCCAGGGG‑GGGCAAT‑CCACGCCCCCCCCCGCC‑GTTCCCGc                                        >  2:2531978/1‑150 (MQ=255)
                                                                                                                tGCAGGAAAGCTAAGGGACTTAGCCTGCGGCGGG‑TTTTTTTTTGT‑TTTGCAACC‑GGGTTG‑G‑CCCCCTTTATTTGTCCCCCCCTAT‑AAAATTAAT‑TGTGG‑AAGGGGGGCGCCGGGGGGG‑GGCCAAC‑CCCCCCCCCCCCCCCCG‑TTCCCGGc                                        >  2:2050250/1‑150 (MQ=255)
                                                                                                                  cAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGC‑TTCAGCAGC‑GGGGTGGG‑CTCCCTTTATGTGGCTCGCCCTAT‑AAATTTTAT‑GGGGG‑AGGGGGCGCG‑CAGGGGGG‑GGGCCAA‑CGCCCCCCCCCCCCGCC‑GTCCCGGccct                                     >  2:1512501/1‑148 (MQ=255)
                                                                                                                    ggAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGCCTCGCCATAT‑AAATTGAAT‑GGTGC‑AGGGAGCGCG‑CAGGGGGC‑GGCCAAT‑CGCCGCCGCCCCCTGCT‑GTCCCGGCCTTCg                                   <  1:2204043/150‑1 (MQ=255)
                                                                                                                     gAAAGCTTAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CCCCCTTAATGTGCCTCGCCATAT‑AAATTGAAT‑GGTCC‑AGGGAGCACG‑CAGGGGGC‑GGGCAAC‑CGCCGCCGCCCCCTGCT‑GTAACGGCCTTCgg                                  <  2:3540940/150‑1 (MQ=255)
                                                                                                                      aaaGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGT‑TTTAACAGCGGGGTGGGG‑CTCCCTT‑ATTTGGCTCCCCCTAT‑AAATTTTAT‑GGGGCAAGGGGGCGCG‑‑AGGGGGG‑GGGCAAT‑TCCCCCCGCCCCCCGCTGGCCCCGCCCTTCgg                                  >  2:2984430/1‑150 (MQ=255)
                                                                                                                       aaGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGCCTCGCCATAT‑AAATTGAAT‑GGTGC‑AGGGAGCGCG‑CAGGGGGC‑GGCCAAT‑CGCCGCCGCCCCCTGCT‑GTCCCGGCCTTCgggg                                <  1:1365567/150‑1 (MQ=255)
                                                                                                                         gCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGC‑TTTAGCAGC‑GGGGTGCG‑CTCCCTTTATGTGCCTCGCCCTAT‑AAAATTAAT‑TGTGC‑AGGGGGCGCG‑CCGGGGGC‑GGCCAAA‑CGCCGCCCCCCCCCGCT‑GTCCCCGCCTTCggggga                              >  2:3392602/1‑148 (MQ=255)
                                                                                                                          cTAAGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGC‑TTTCCCGGC‑GGGTGTTG‑TTCCCCTTATGTGCCTCGCCGCATAAAATGGAAA‑GGGGC‑‑GGGGGCACG‑CCGGGGGC‑GGCCCAT‑CGCCCACGCCCCCCGCC‑GTCCCCGCCCTCgggggaa                             >  2:2706882/1‑147 (MQ=255)
                                                                                                                            aaGGGACTTAGCCTGCGGC‑GG‑TTTTTTTTTGG‑TTCCGCAGC‑GGGGGGGG‑CTCCCCTTATTTGCCTCCCCCTAT‑AAATTTAAT‑GGGGG‑CGGGGGCGCG‑CCGGGGGG‑GGCCCAT‑CGCCCCCGCCCCCCCCT‑GTCCCGGCCCTCggggggcgg                           >  2:3833139/1‑145 (MQ=255)
                                                                                                                                 aCTTAGCCTGCGGC‑GG‑TTTTTTTTTGG‑TTCCGCCGC‑GGGGTGCG‑CGCCCTTTATTTGGCCTGCCATTT‑TAATTTGAT‑TGTGG‑CGGGGGGGCG‑CAAGGGGC‑GGGCAATCCCCCCCCCCCCCCTCCG‑TTCCCGCCCTTGGGGGAACCCtccg                       >  2:499238/1‑147 (MQ=255)
                                                                                                                                   ttAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGCCTCGCCATAT‑AAATTGAAT‑GGTGC‑AGGGAGCGCG‑CAGGGGGC‑GGCCAAT‑CGCCGCCGCCCCCTGCT‑GTCCCGGCCTTCGGGGAACGCTTCAGCg                    <  1:3859033/150‑1 (MQ=255)
                                                                                                                                         tGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGCCTCGCCATAT‑AAATTGAAT‑GGTGC‑AGGGAGCGCG‑CAGGGGGC‑GGCCAAT‑CGCCGCCGCCCCCTGCT‑GTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTg              <  1:828334/150‑1 (MQ=255)
                                                                                                                                           cggc‑gg‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGCCTCGCCATAT‑AAATTGAAT‑GGTGC‑AGGGAGCGCG‑CAGGGGGC‑GGCCAAT‑CGCCGCCGCCCCCTGCT‑GTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGAc            <  1:1143174/150‑1 (MQ=255)
                                                                                                                                                gg‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGCCTCGCCATAT‑AAATTGAAT‑GGTGC‑AGGGAGCGCG‑CAGGGGGC‑GGCCAAT‑CGCCGCCGCCCCCTGCT‑GTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGcca        >  2:3627938/1‑150 (MQ=255)
                                                                                                                                                    tttgtttgGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGCCTCGCCATAT‑AAATTGAAT‑GGTGC‑AGGGAGCGCG‑CAGGGGGC‑GGCCAAT‑CGCCGCCGCCCCCTGCT‑GTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGccacca     <  2:3211930/150‑1 (MQ=255)
                                                                                                                                                     ttgtttgGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGCCTCGCCATAT‑AAATTGAAT‑GGTGC‑AGGGAGCGCG‑CAGGGGGC‑GGCCAAT‑CGCCGCCGCCCCCTGCT‑GTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCaa    >  1:4146737/1‑150 (MQ=255)
                                                                                                                                                      tgtttgGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGCCTCGCCATAT‑AAATTGAAT‑GGTGC‑AGGGAGCGCG‑CAGGGGGC‑GGCCAAT‑CGCCGCCGCCCCCTGCT‑GTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAac   <  2:3759752/150‑1 (MQ=255)
                                                                                                                                                      tgtttgGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGCCTCGCCATAT‑AAATTGAAT‑GGTGC‑AGGGAGCGCG‑CAGGGGGC‑GGCCAAT‑CGCCGCCGCCCCCTGCT‑GTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAac   >  2:3376085/1‑150 (MQ=255)
                                                                                                                                                       gtttgGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGCCTCGCCATAT‑AAATTGAAT‑GGTGC‑AGGGAGCGCG‑CAGGGGGC‑GGCCAAT‑CGCCGCCGCCCCCTGCT‑GTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAaca  <  1:3037691/150‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                               
GGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATGCAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAGGAAAGCTAAGGGACTTAGCCTGCGGC‑GG‑TTTTGTTTGGC‑TTCAGCAGC‑GGGTTGCG‑CTCCCTTAATGTGCCTCGCCATAT‑AAATTGAAT‑GGTGC‑AGGGAGCGCG‑CAGGGGGC‑GGCCAAT‑CGCCGCCGCCCCCTGCT‑GTCCCGGCCTTCGGGGAACGCTTCAGCGATTTTGACGCCACCAACA  >  AP012306/934905‑935203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑