Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,066,3230AC21.8% 669.2 / 14.8 229intergenic (‑92/+33)ychM/prsApredicted transporter/phosphoribosylpyrophosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (162/17);  new base C (0/50);  total (162/67)
Fisher's exact test for biased strand distribution p-value = 3.39e-36
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 267 total aligned reads displayed.

AGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCT  >  AP012306/1066174‑1066472
                                                                                                                                                      |                                                                                                                                                     
 gCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAAc                                                                                                                                                      >  1:2478650/1‑150 (MQ=255)
   aaGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAAccc                                                                                                                                                    <  2:3342667/150‑1 (MQ=255)
             cAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCgg                                                                                                                                          >  1:3792548/1‑150 (MQ=255)
             cAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCgg                                                                                                                                          >  2:166881/1‑150 (MQ=255)
                  cgcgGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGGCtt                                                                                                                                     >  2:955267/1‑150 (MQ=255)
                  cgcgGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGGCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCtt                                                                                                                                     >  2:2587007/1‑150 (MQ=255)
                    gggAAAGGCATCAAATGTGAGAAAAATATTTTGTTCAAAAAAATTTTTCACCCGTGGGCGCATCATCTGTCAACAAAAGGGCAGGGGAGGGAGGAATAGGTAAAACAAATGG‑ATATAAAAGAAAAAAAAACCCGCCGCAGCGGGTCTTTg                                                                                                                                   <  2:1964257/149‑1 (MQ=255)
                    cgGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTg                                                                                                                                   >  2:476520/1‑150 (MQ=255)
                         aGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCg                                                                                                                              >  2:4054114/1‑150 (MQ=255)
                         aGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCg                                                                                                                              >  2:3788885/1‑150 (MQ=255)
                          ggCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCgg                                                                                                                             >  2:3189829/1‑150 (MQ=255)
                           gCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCAGTGAGCGCATCATCTGTCAACTAAATGGCAGGAGAAGGAGGCCTAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCCCAGCGGGGCTTTGAGCCggg                                                                                                                            >  2:2896463/1‑150 (MQ=255)
                               cacaTGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCg                                                                                                                        >  2:3814769/1‑150 (MQ=255)
                               cacaTGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCg                                                                                                                        >  2:3002008/1‑150 (MQ=255)
                               cacaTGTGAGAAAAACATTTTTTTCAAAATAATTTTTCATCCGGGAGCGCATCATCTGTCAACAAAAGGGCAGGGGAAGGGGGCATAGGTCATAAAAAGGG‑ATATTACAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCg                                                                                                                        <  2:3611402/150‑1 (MQ=255)
                                    gtgAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAg                                                                                                                   >  1:617018/1‑150 (MQ=255)
                                     tgAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTAAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAgt                                                                                                                  >  1:3063498/1‑150 (MQ=255)
                                     ggagaAAAATTTTTTGTTCAAAATAATTTTTCACCCGTGAGCGCATCATCTGTCAACAAAAGGGCAGGGGAAGGAGGCATAGGTCATAAAAAGGG‑ATATTAAAGAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAgt                                                                                                                  <  2:2790008/149‑1 (MQ=255)
                                      gAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAgtg                                                                                                                 >  1:2404478/1‑150 (MQ=255)
                                      gAGGAAAATATTTTGTTAAAAATATTGTTGAACCGGTGGGCGCATCACCTCTCAACTAAATGGGGGGTGAAGGAGGCATAGTTCATACAAATGG‑ATATTAAAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAgtg                                                                                                                 <  2:626266/150‑1 (MQ=255)
                                       aGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAgtgt                                                                                                                >  1:3280913/1‑150 (MQ=255)
                                          aaaaTATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCg                                                                                                             >  2:1549618/1‑150 (MQ=255)
                                           aaaTATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGa                                                                                                            >  2:4314866/1‑150 (MQ=255)
                                           aaaTATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGa                                                                                                            >  2:1165867/1‑150 (MQ=255)
                                              tatTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACa                                                                                                         >  2:1437083/1‑150 (MQ=255)
                                               atTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACAt                                                                                                        >  1:3586665/1‑150 (MQ=255)
                                                 tttGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATgg                                                                                                      >  1:2360450/1‑150 (MQ=255)
                                                 tttGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATgg                                                                                                      >  1:4187995/1‑150 (MQ=255)
                                                   tGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCa                                                                                                    >  1:151200/1‑150 (MQ=255)
                                                   tGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCa                                                                                                    >  1:1132149/1‑150 (MQ=255)
                                                    gTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCag                                                                                                   >  1:4094131/1‑150 (MQ=255)
                                                     ttCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCaga                                                                                                  >  2:4418141/1‑150 (MQ=255)
                                                      tCAAAAAAATGTTTAATCCGTAAGACCATCATCTGTCACCAAAAGGGAGGGTAAAGGGGCCTTAGGTCATA‑AAATGGTATATTACAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCagag                                                                                                 <  2:1099433/150‑1 (MQ=255)
                                                        acaATAATGTTTCATCCGTGAGCGCATCATCTGTCAACAAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGAt                                                                                               <  1:3988125/150‑1 (MQ=255)
                                                         caATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATc                                                                                              >  2:2083692/1‑150 (MQ=255)
                                                          aataatGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGGGATCg                                                                                             >  2:2180808/1‑150 (MQ=255)
                                                           ataatGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGa                                                                                            >  2:789086/1‑150 (MQ=255)
                                                            taatGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAt                                                                                           >  1:3343603/1‑150 (MQ=255)
                                                             aatGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAtt                                                                                          >  2:2312201/1‑150 (MQ=255)
                                                             aatGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAtt                                                                                          >  1:537272/1‑150 (MQ=255)
                                                                gTTCCATCCGTGAGCGCATCATCTGTAAACAAAAGGGCAGGTGAAGGAGGAATAGGTAATAAAAATGG‑ATATAACAGAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAttctt                                                                                       <  2:2284881/150‑1 (MQ=255)
                                                                  ttCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCTGCAGCGGGTCTTTGGGCCGGGTTCGATTAGTGTTCACACATGGCAGAGGTCGGTTTTTCg                                                                                     >  2:2974308/1‑150 (MQ=255)
                                                                  ttCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCg                                                                                     >  2:4291506/1‑150 (MQ=255)
                                                                    cATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGtt                                                                                   >  1:2906696/1‑150 (MQ=255)
                                                                    cATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGtt                                                                                   >  1:1758610/1‑150 (MQ=255)
                                                                    cATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATAATACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGtt                                                                                   >  2:3170016/1‑150 (MQ=255)
                                                                     aTCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTg                                                                                  >  1:1255509/1‑150 (MQ=255)
                                                                      tCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGc                                                                                 >  2:840613/1‑150 (MQ=255)
                                                                         tggAGCGCATCATCTGTAAACAAAAGGGAGGGTGAAGGGGGAATGGGTAATAAAAATGA‑ATATAACAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGa                                                                              <  2:2424614/148‑1 (MQ=255)
                                                                         gTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGa                                                                              >  1:1940823/1‑150 (MQ=255)
                                                                             gcgcATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATacg                                                                          >  1:4247071/1‑150 (MQ=255)
                                                                              cgcATCATCTGTCAAAAAAAGGGAAGGGGAGGGGGGAATAGGTAAAAAAAAGGG‑ATATTACAGAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATacga                                                                         <  2:3920631/150‑1 (MQ=255)
                                                                                 atcatcTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATAcgacga                                                                      >  2:3874645/1‑150 (MQ=255)
                                                                                    atcTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATc                                                                   >  1:2100300/1‑150 (MQ=255)
                                                                                      cTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGc                                                                 >  1:1978663/1‑150 (MQ=255)
                                                                                          cactaaaaGGGCGGGGGAGGGGGGCATAGGCAATAAAAATGG‑ATTTTAAAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCg                                                             <  2:3419177/145‑1 (MQ=255)
                                                                                              tAAATGGCAGGTGAAGGAGGCATAGGGCATACAAATGG‑ATATTAAAGACAAAAAAACCCGCCGCAGCGGGGCTTTGAGCCGGGTTCGATTAGGGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCa                                                         >  2:3454026/1‑150 (MQ=255)
                                                                                                   ggCAGGTGAAGGAGGCATGGGGCATACAAATGG‑ATATTACAGAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATa                                                    <  1:3877806/150‑1 (MQ=255)
                                                                                                     cAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATAACAGAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATAcc                                                  <  1:2592594/150‑1 (MQ=255)
                                                                                                         tGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGAc                                              >  1:1014079/1‑150 (MQ=255)
                                                                                                         tGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGAc                                              >  2:1043309/1‑150 (MQ=255)
                                                                                                          gAAGGAGGCATAGGTAATAAAAATGG‑ATATTAAAGAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACa                                             <  1:2804256/150‑1 (MQ=255)
                                                                                                           aaGGGGGCATAGTTCATAAAAAGGA‑ATATTACAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAg                                            <  2:553768/150‑1 (MQ=255)
                                                                                                               gggCATAGGTCATAAAAATGA‑ATATAAAAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTc                                        <  2:2483632/149‑1 (MQ=255)
                                                                                                               aggCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTc                                        >  1:4027498/1‑150 (MQ=255)
                                                                                                                ggCATAGGTCATACAAATGA‑ATATTACAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCa                                       <  1:3374847/150‑1 (MQ=255)
                                                                                                                  cATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCaga                                     >  1:1069611/1‑150 (MQ=255)
                                                                                                                   aTAGGTCAGACAATTGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTTTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCATCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAAGGTCagag                                    >  2:2056026/1‑150 (MQ=255)
                                                                                                                    tAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGt                                   >  1:294821/1‑150 (MQ=255)
                                                                                                                    aaGGTAATAAAAAGGA‑ATATAACAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGt                                   <  1:3291176/149‑1 (MQ=255)
                                                                                                                      ggTAATACAAAGGG‑ATATTACAGAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTAc                                 <  1:2149996/150‑1 (MQ=255)
                                                                                                                        tCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGc                               >  1:802047/1‑150 (MQ=255)
                                                                                                                            aCAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGt                           >  1:309320/1‑150 (MQ=255)
                                                                                                                            aCAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGCACGCACGt                           >  1:4327496/1‑150 (MQ=255)
                                                                                                                             cAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGtt                          >  1:344077/1‑150 (MQ=255)
                                                                                                                             cAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGtt                          >  1:2573298/1‑150 (MQ=255)
                                                                                                                              aaaTGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTc                         >  2:4134446/1‑150 (MQ=255)
                                                                                                                               aaTGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCg                        >  2:2632015/1‑150 (MQ=255)
                                                                                                                               aaTGG‑ATATAACAGACAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCg                        <  2:1314920/150‑1 (MQ=255)
                                                                                                                               aaTGA‑ATATAAAAAAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCg                        <  2:2990691/150‑1 (MQ=255)
                                                                                                                                 tGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGc                      >  1:2249994/1‑150 (MQ=255)
                                                                                                                                 tGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGc                      >  1:4397456/1‑150 (MQ=255)
                                                                                                                                      tatTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtg                  >  1:2700916/1‑150 (MQ=255)
                                                                                                                                      aatTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtg                  <  1:1330927/149‑1 (MQ=255)
                                                                                                                                       atTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtga                 >  2:727030/1‑150 (MQ=255)
                                                                                                                                         tataaaCAAAAAATCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtgatt               <  1:914822/145‑1 (MQ=255)
                                                                                                                                         tACAAACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtgatt               <  1:1388208/150‑1 (MQ=255)
                                                                                                                                         aacaaaaaaaaaacCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtgatt               <  2:1821639/143‑1 (MQ=255)
                                                                                                                                          acaaaaaaaaaacCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtgattt              <  2:3695320/144‑1 (MQ=255)
                                                                                                                                          aaaaaaaaaaaacCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtgattt              <  2:1782621/144‑1 (MQ=255)
                                                                                                                                          aCAGAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtgattt              <  1:453978/150‑1 (MQ=255)
                                                                                                                                             gaaaaaaaacCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtgatttgat           <  2:161253/147‑1 (MQ=255)
                                                                                                                                              aCAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtgatttgatt          >  1:1459396/1‑150 (MQ=255)
                                                                                                                                                aaaaaacCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTc        <  2:3601229/150‑1 (MQ=255)
                                                                                                                                                aaaaaaaCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTc        <  1:721417/150‑1 (MQ=255)
                                                                                                                                                 aaaaaaCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCa       >  1:685629/1‑150 (MQ=255)
                                                                                                                                                 aaaaaaCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCa       >  2:3553038/1‑150 (MQ=255)
                                                                                                                                                   aaacCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATc     <  1:1230504/150‑1 (MQ=255)
                                                                                                                                                    aaaCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCg    >  2:3059562/1‑150 (MQ=255)
                                                                                                                                                      aCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCt  >  1:323032/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
AGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGG‑ATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCT  >  AP012306/1066174‑1066472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑