Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG47.2% 368.5 / 93.2 269intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
Reads supporting (aligned to +/- strand):  ref base T (39/103);  new base G (127/0);  total (166/103)
Fisher's exact test for biased strand distribution p-value = 4.87e-42
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 291 total aligned reads displayed.

GCTGGGGATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGT  >  AP012306/1091843‑1092141
                                                                                                                                                     |                                                                                                                                                     
gCTGGGGATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGt                                                                                                                                                       <  2:886986/150‑1 (MQ=255)
 cTGGGGATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGtt                                                                                                                                                      <  1:3105643/150‑1 (MQ=255)
  tGGGGATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGttt                                                                                                                                                     <  1:4281673/150‑1 (MQ=255)
    gggATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGttttt                                                                                                                                                   >  1:1273760/1‑150 (MQ=255)
    gggATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGgtttt                                                                                                                                                   >  2:3057003/1‑150 (MQ=255)
       aTGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTa                                                                                                                                                >  1:2662847/1‑150 (MQ=255)
        tGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTAt                                                                                                                                               >  1:2397171/1‑150 (MQ=255)
         gACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATg                                                                                                                                              >  1:817200/1‑150 (MQ=255)
          aCGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGgtttttttttg                                                                                                                                             >  2:2136145/1‑146 (MQ=255)
          aCGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTAACAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCCGGGTTTTTTTATgg                                                                                                                                             >  1:785667/1‑150 (MQ=255)
           cGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTTTgtg                                                                                                                                            >  1:1931841/1‑148 (MQ=255)
             ttATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCa                                                                                                                                          >  1:4112341/1‑150 (MQ=255)
              tATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCAc                                                                                                                                         >  1:1133023/1‑150 (MQ=255)
              tATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCAc                                                                                                                                         >  2:411537/1‑150 (MQ=255)
                 tCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGt                                                                                                                                      <  2:688405/150‑1 (MQ=255)
                 tCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACggg                                                                                                                                      >  1:248911/1‑149 (MQ=255)
                  ccTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTg                                                                                                                                     <  1:2463215/150‑1 (MQ=255)
                  ccTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTg                                                                                                                                     >  1:2268399/1‑150 (MQ=255)
                   ctgctgTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTTTGGGCACGGTGc                                                                                                                                    >  2:756268/1‑150 (MQ=255)
                      ctgTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTTTGGGCACGGGGCggg                                                                                                                                 >  2:819680/1‑150 (MQ=255)
                       tgTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCgggg                                                                                                                                <  2:3888855/150‑1 (MQ=255)
                        gTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGt                                                                                                                               <  2:1354839/150‑1 (MQ=255)
                         ttCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGTg                                                                                                                              >  1:1813264/1‑150 (MQ=255)
                           cccGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGTGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTTTTGGCACGGTgggggggggg                                                                                                                            >  2:122653/1‑146 (MQ=255)
                            ccGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGt                                                                                                                           <  1:3909251/150‑1 (MQ=255)
                            ccGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGt                                                                                                                           >  2:1674873/1‑150 (MQ=255)
                              gTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGGTg                                                                                                                         >  2:654909/1‑150 (MQ=255)
                                    gcgTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTTTTGGCACGGGGCGGGGGGAGGTTTCgggt                                                                                                                   >  2:2631525/1‑148 (MQ=255)
                                    gcgTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATTGGCACGGTGCGGGGGGAGTTGTCGGAt                                                                                                                   >  2:104017/1‑150 (MQ=255)
                                    gcgTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGAt                                                                                                                   >  2:2879968/1‑150 (MQ=255)
                                                 aTCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTTTCGGATGCGCTTCGCTTAt                                                                                                      >  1:3588854/1‑150 (MQ=255)
                                                 aTCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGGTTTTTTTGGGGACGGGGGCGGGGGGGCTGTCGGGTGCGCCTCGCTTAt                                                                                                      >  2:3274937/1‑150 (MQ=255)
                                                  tCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGGTGTCGGATGGGCTTCGCTTTTc                                                                                                     >  2:2012721/1‑150 (MQ=255)
                                                   cTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATcc                                                                                                    <  1:1591834/150‑1 (MQ=255)
                                                       aGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGAcc                                                                                                <  2:1533183/150‑1 (MQ=255)
                                                         cGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTa                                                                                              >  2:2973933/1‑150 (MQ=255)
                                                            aCCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAg                                                                                           <  1:2162318/150‑1 (MQ=255)
                                                             ccGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAgg                                                                                          >  2:3776139/1‑150 (MQ=255)
                                                                gTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGgga                                                                                       >  1:2823274/1‑150 (MQ=255)
                                                                   gAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGggagga                                                                                    >  1:3794144/1‑150 (MQ=255)
                                                                   gAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGGGCGGGGTGAGCTGTCGGATGCGCTTCGCTTATCCGACCTACAGGggaggg                                                                                    >  2:4023227/1‑149 (MQ=255)
                                                                    aGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGGTGTCGGGTGCGCTTCGCTTTTTCGACCTACAGGGGGGGat                                                                                   >  2:3326199/1‑150 (MQ=255)
                                                                      tATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGatat                                                                                 <  1:1176646/150‑1 (MQ=255)
                                                                         cTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGGTATTGt                                                                              >  1:4386117/1‑150 (MQ=255)
                                                                            acacGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTatg                                                                           <  2:1603598/150‑3 (MQ=255)
                                                                            acacGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGGGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGGTATTTTAgg                                                                           >  1:320962/1‑150 (MQ=255)
                                                                             cacGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGGGGGTATTGTAGGc                                                                          >  2:1547207/1‑150 (MQ=255)
                                                                               cGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGGGGGTATTGTAGGccc                                                                        >  2:1263291/1‑150 (MQ=255)
                                                                                    gTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTaa                                                                   <  2:2365906/150‑1 (MQ=255)
                                                                                      aCCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAAc                                                                 <  2:3547156/150‑1 (MQ=255)
                                                                                       ccAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGGTATTGTAGGCCCGGTAAACg                                                                >  1:2589191/1‑150 (MQ=255)
                                                                                          gCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACgtgg                                                             >  2:560546/1‑150 (MQ=255)
                                                                                           cTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACgtggt                                                            <  2:3922284/150‑1 (MQ=255)
                                                                                           cTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACgtggt                                                            <  1:2896841/150‑1 (MQ=255)
                                                                                              gTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGcc                                                         <  1:3627697/150‑1 (MQ=255)
                                                                                               tGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCa                                                        <  2:414079/150‑1 (MQ=255)
                                                                                                 cgcgcTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATc                                                      <  2:1466156/150‑1 (MQ=255)
                                                                                                    gcTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCggg                                                   <  2:1769092/150‑1 (MQ=255)
                                                                                                    gcTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCggg                                                   <  2:2805994/150‑1 (MQ=255)
                                                                                                        gTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGGTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGGGGGTATTTTAGGGCCGGGAAACGGGGGGCCATCGGGCcaa                                               >  2:855981/1‑150 (MQ=255)
                                                                                                         tCACTAAGCGAAGTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATTGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGGGGGTATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCaaaa                                              >  2:4328702/1‑150 (MQ=255)
                                                                                                          cACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAAt                                             >  1:2782886/1‑150 (MQ=255)
                                                                                                           aCTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAAtt                                            <  1:1308710/150‑1 (MQ=255)
                                                                                                           aCTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAAtt                                            >  1:1487586/1‑150 (MQ=255)
                                                                                                            cTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGGGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGGGGGTATTGTAGGCCCGGGAAACGTGGGGCCATCCGGCAAAATTa                                           >  2:3462544/1‑150 (MQ=255)
                                                                                                                  gAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCgg                                     >  1:1364117/1‑150 (MQ=255)
                                                                                                                   aaTTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGa                                    >  1:586109/1‑150 (MQ=255)
                                                                                                                    aTTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAg                                   >  1:407505/1‑150 (MQ=255)
                                                                                                                    aTTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATTGGGACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGGGGGTATTGTAGGGCCGGTAAACGTGGGGCCATCGGGCAAAATTAGCTCgggg                                   >  2:1405098/1‑148 (MQ=255)
                                                                                                                    aTTTTAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGGGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGGGGATATTGTAGGCCCGGGAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAg                                   >  2:3404083/1‑150 (MQ=255)
                                                                                                                     ttttAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGGGCGGGGGGGGTTGTCGGGTGGGCTTCGCTTATCCGACCTACAGGGGGGGATATTGTAGGCCCGGTAAAAGGGGTGCCATCGGGGAAAATTAGGTCGGAgg                                  >  2:925081/1‑149 (MQ=255)
                                                                                                                        tAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAAc                               <  2:4317012/150‑1 (MQ=255)
                                                                                                                        tAGTTCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGGGGGTATTGTAGGCCCGGGAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGGAAc                               >  2:2835880/1‑150 (MQ=255)
                                                                                                                         aGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGGGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGGGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGGAACa                              >  1:4058826/1‑150 (MQ=255)
                                                                                                                           ttCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGGACGGGGCGGGGGGAGTTGTCGGGTGGGCTTCGCTTATCCGACCTAAAGGGGGGGGTATTGTAGGGCCGGTAAAAGTGGGGCCATCGGGCAAAATTAGGTCGGAGGAACAgg                            >  2:3690874/1‑150 (MQ=255)
                                                                                                                            tCACATAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGGGCGGGGGGAGGTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGGGGGTATTGTAGGCCCGGGAAACGGGGGGCCATCCGGGGAAATTAGCTCGGGGGAACAggg                           >  2:4413927/1‑149 (MQ=255)
                                                                                                                             cacaTAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGtt                          <  2:3223114/150‑1 (MQ=255)
                                                                                                                              acaTAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGttt                         <  1:825167/150‑1 (MQ=255)
                                                                                                                              acaTAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGttt                         <  2:3658957/150‑1 (MQ=255)
                                                                                                                              acaTAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGGGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGCACTACAGGGGGGGGTATTGTAGGCCCGGGAAACGGGGGGCCATCGGGCAAAATTAGCTCGGAGGAACAGGgtt                         >  2:1449112/1‑150 (MQ=255)
                                                                                                                              acaTAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGGGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGgtt                         >  1:1728878/1‑150 (MQ=255)
                                                                                                                                aTAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTg                       <  1:2308995/150‑1 (MQ=255)
                                                                                                                                aTAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTg                       <  1:3657206/150‑1 (MQ=255)
                                                                                                                                 tAGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGGTATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGGGGAACAGGGTTTGa                      >  2:2531259/1‑150 (MQ=255)
                                                                                                                                  aGACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGTGCGGGGGGAGTTGTCGGATGCGCTTCGCTTATTCGACCCACAGGGGAGGGTATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGGGTAACAGGTTTTGat                     >  2:75862/1‑150 (MQ=255)
                                                                                                                                   gACCCTGCTTCGGCGGGGGTTTTTTATGGGCACGGGGCGGGGGGGGTTGTCGGATGCGCTTCGCTTATTCGACCTACAGGGGGGGGTATTGTAGGCCCGGGAAACGTGGTGCCATCGGGCAAAATTAGCTCGGGGGAACAGGGTTTGatt                    >  2:1811393/1‑150 (MQ=255)
                                                                                                                                     cccTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGattat                  <  2:3579069/150‑1 (MQ=255)
                                                                                                                                       cTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTAttt                <  1:947615/150‑1 (MQ=255)
                                                                                                                                         gCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTaa              <  2:162194/150‑1 (MQ=255)
                                                                                                                                         gCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTaa              <  2:3165373/150‑1 (MQ=255)
                                                                                                                                          cTTCGGCGGGGTTTTTTTTTTGGCACGGTGGGGGGGGGGTTGGCGGGTGGGCTTCGCTTATCCGACCCACAAGGGAGGATATTGTAGGCCCGGTAAAACTGGTGCCATCGGGCGAAATTAGCTCCGGGTAACAGGTTTTGATTTTTTTAg             >  2:3819940/1‑150 (MQ=255)
                                                                                                                                           ttCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAgg            <  1:3409288/150‑1 (MQ=255)
                                                                                                                                           ttCGGCGGGGGTTTTTTTTGGGCACGGGGCGGGGGGGGTTGTCGGGTGCGCCTCGCTTATCCGACCTACAGGGGGGGGTATTGTAGGCCCGGTGAAAGGGGGGCCATCGGGGAAAATTAGCTCGGGGGAACAGGGTTTGATTATTTTAgg            >  2:189732/1‑150 (MQ=255)
                                                                                                                                            tCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGa           <  1:572429/150‑1 (MQ=255)
                                                                                                                                            tCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGa           <  1:48629/150‑1 (MQ=255)
                                                                                                                                             cggcggGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGaa          <  1:4130576/150‑1 (MQ=255)
                                                                                                                                             cggcggGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGaa          <  1:349780/150‑1 (MQ=255)
                                                                                                                                                 ggggTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGa      >  1:4217528/1‑150 (MQ=255)
                                                                                                                                                   ggTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGAtg    <  1:2953406/150‑1 (MQ=255)
                                                                                                                                                    gTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGAtgg   >  1:923162/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCTGGGGATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTGGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTTGTCGGATGCGCTTCGCTTATCCGACCTACAGGGGAGGATATTGTAGGCCCGGTAAACGTGGTGCCATCGGGCAAAATTAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGT  >  AP012306/1091843‑1092141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑