Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,558,0710AC21.9% 608.6 / 17.0 233V302G (GTA→GGA) visCpredicted oxidoreductase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base A (129/53);  new base C (0/51);  total (129/104)
Fisher's exact test for biased strand distribution p-value = 1.65e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 279 total aligned reads displayed.

CTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAA  >  AP012306/2557923‑2558220
                                                                                                                                                    |                                                                                                                                                      
    aTACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTAccccc                                                                                                                                                   <  2:2610986/150‑1 (MQ=255)
    aTACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTAccccc                                                                                                                                                   >  2:2762793/1‑150 (MQ=255)
     tACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCt                                                                                                                                                  >  1:3330484/1‑150 (MQ=255)
     tACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCt                                                                                                                                                  >  1:1202516/1‑150 (MQ=255)
       ccagcctgCATCAACGCCGCAATGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCGGACGATGCAACCGTTTCAGTTCGGCAATCAGTTCTGCAGCATCCATAAAGCCGAGATTTACCCTCTG‑c                                                                                                                                               <  2:1900102/150‑1 (MQ=255)
         agccagcATCAACGCCGCACTGTGCTTTCGGCTACCCTCATAGGGCCGCAGATAAATTTACTGCCCGCTGTCTTTCCCCTGACGAGGCAACCCTTTTAGTTCGGCAATCAGCTCTGCCGCCTCCATAAAGCCGGGATTTACCCCCTG‑ccc                                                                                                                                             >  2:2462248/1‑150 (MQ=255)
         agccagcATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑ccc                                                                                                                                             <  2:4346949/150‑1 (MQ=255)
                   aaCGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCggg                                                                                                                                   >  1:2093070/1‑150 (MQ=255)
                      gccgcACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGa                                                                                                                                >  1:4085342/1‑150 (MQ=255)
                       ccgcACTGTGCTGGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTG‑CCCCGCCAGCGGGTGaa                                                                                                                               <  1:943875/150‑1 (MQ=255)
                       ccccACTGTGCTTGGGGCTACGCTCATAGCGACGAAGATAAATGTACTGCCCGATGTCTTCCCCCTGACGATGCACCCGTTTCAGTTCGGCAATCAGCTCGGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGaa                                                                                                                               <  2:3962052/147‑1 (MQ=255)
                        cgcACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAAt                                                                                                                              >  1:1376458/1‑150 (MQ=255)
                        cgcACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAAt                                                                                                                              <  1:1540273/150‑1 (MQ=255)
                         gcACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATg                                                                                                                             >  2:1771516/1‑150 (MQ=255)
                         gcACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTCCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATg                                                                                                                             <  1:2579286/150‑1 (MQ=255)
                          cACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATgg                                                                                                                            >  2:1190321/1‑150 (MQ=255)
                           aCTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGt                                                                                                                           >  2:2803249/1‑150 (MQ=255)
                           aCTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGt                                                                                                                           >  2:1845309/1‑150 (MQ=255)
                              gtgCTTGGGGCTACGCTCATAGCGACGCAGATAAATGTCCTGCCCGATGTCTTTCCCCTGCCGATGCACCCGTTTCAGTGCGGCAATCAGCTCTGCAGCATCCAAAAAGCCGAGATTTCCCCCCTG‑CCCCGCCAGCGGGTGAATGGTAtg                                                                                                                        <  2:3021566/150‑1 (MQ=255)
                                gCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTAtgtg                                                                                                                      <  2:2681082/150‑1 (MQ=255)
                                   tGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTCCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTgcgg                                                                                                                   <  2:3519492/150‑1 (MQ=255)
                                        cTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCg                                                                                                              >  1:3758813/1‑150 (MQ=255)
                                            gCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCcca                                                                                                          >  1:743696/1‑150 (MQ=255)
                                              tCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGAGCGGCGTCGCccacc                                                                                                        >  2:3643127/1‑150 (MQ=255)
                                                aTAGCGACGCAGATAAATGTCCTGCCCGATGTCTTCCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAg                                                                                                      <  2:2306371/150‑1 (MQ=255)
                                                aTAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAg                                                                                                      <  2:4063269/150‑1 (MQ=255)
                                                 tAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAgc                                                                                                     >  1:4422257/1‑150 (MQ=255)
                                                 tAGCGACGCAGATAAATGTACTGCCCGATGTCTTCCCCCTGACGATGCACCCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAgc                                                                                                     <  2:3625265/150‑1 (MQ=255)
                                                  aGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAgcg                                                                                                    >  2:1671451/1‑150 (MQ=255)
                                                  aGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAgcg                                                                                                    >  1:3428208/1‑150 (MQ=255)
                                                   gCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAgcgc                                                                                                   <  1:71329/150‑1 (MQ=255)
                                                    cGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGcc                                                                                                  >  2:2515225/1‑150 (MQ=255)
                                                    cGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGcc                                                                                                  <  1:2283048/150‑1 (MQ=255)
                                                     gACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCa                                                                                                 >  1:1430831/1‑150 (MQ=255)
                                                       cGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGa                                                                                               >  1:556342/1‑150 (MQ=255)
                                                            aTAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTg                                                                                          >  2:84061/1‑150 (MQ=255)
                                                            aTAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTg                                                                                          >  1:2465847/1‑150 (MQ=255)
                                                             tAAATGTACTGCCGGATGTCTTTCCCCTGACGATGCAACCTTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGc                                                                                         <  2:1309175/150‑1 (MQ=255)
                                                              aaaTTTACTGCCCGATGTCTTCCCCCTGACGATGCAACCTTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCg                                                                                        <  2:70453/150‑1 (MQ=255)
                                                              aaaTGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCg                                                                                        >  2:3874517/1‑150 (MQ=255)
                                                               aaTGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGa                                                                                       >  1:1776753/1‑150 (MQ=255)
                                                                      tGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAAc                                                                                <  1:3884576/150‑1 (MQ=255)
                                                                          cGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGgc                                                                            >  1:3203535/1‑150 (MQ=255)
                                                                           gATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGgcg                                                                           >  1:1959339/1‑150 (MQ=255)
                                                                            aTGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGgcgc                                                                          >  2:1384555/1‑150 (MQ=255)
                                                                                cTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCAt                                                                      >  2:1967175/1‑150 (MQ=255)
                                                                                 tttCCCCTGACGATGCACCCGTTTCAGTTCGGAAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATa                                                                     <  1:3826787/150‑1 (MQ=255)
                                                                                 tttCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATa                                                                     >  2:3040483/1‑150 (MQ=255)
                                                                                 tttCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATa                                                                     >  2:1535885/1‑150 (MQ=255)
                                                                                 ttcccccTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATa                                                                     <  1:3105253/147‑1 (MQ=255)
                                                                                   tCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAAc                                                                   >  2:2229937/1‑150 (MQ=255)
                                                                                       cTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGccc                                                               <  1:3038892/150‑1 (MQ=255)
                                                                                        tGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGcccc                                                              >  2:652392/1‑150 (MQ=255)
                                                                                        tGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGcccc                                                              >  2:3158861/1‑150 (MQ=255)
                                                                                          aCGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGt                                                            <  1:162925/150‑1 (MQ=255)
                                                                                            gATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCa                                                          >  2:428536/1‑150 (MQ=255)
                                                                                            gATGAAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCa                                                          <  2:477274/150‑1 (MQ=255)
                                                                                               gaaaCCGTTCAAGTTCGCCAACCGGCTCTCCAGAACCCAAAAAGCGGAG‑TTTACCCCCCGCCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTg                                                       <  2:2000350/148‑1 (MQ=255)
                                                                                                   ccGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGaa                                                   >  2:1125042/1‑150 (MQ=255)
                                                                                                    cGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAac                                                  >  2:880822/1‑150 (MQ=255)
                                                                                                    cGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAac                                                  >  2:2150544/1‑150 (MQ=255)
                                                                                                       ttCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTGAGTGGGAACAcc                                               >  2:2883391/1‑150 (MQ=255)
                                                                                                       ttCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACAcc                                               >  2:4395843/1‑150 (MQ=255)
                                                                                                         cAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGAGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTg                                             >  2:1587504/1‑150 (MQ=255)
                                                                                                           tttCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGAc                                           <  2:591366/149‑1 (MQ=255)
                                                                                                           gTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGAc                                           <  1:1478738/150‑1 (MQ=255)
                                                                                                           gTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCAGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGAc                                           <  2:3033414/150‑1 (MQ=255)
                                                                                                            ttCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTCCCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGCGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGAcg                                          <  1:3556537/150‑1 (MQ=255)
                                                                                                             tCGGCAATCAGCTCGGCAGCATCCATAAAGCCGAGTTTTCCCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGAcgc                                         <  2:1441655/150‑1 (MQ=255)
                                                                                                              cGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGAcgcg                                        >  1:2740445/1‑150 (MQ=255)
                                                                                                                 cAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACgcgcgc                                     >  1:2233124/1‑150 (MQ=255)
                                                                                                                     cAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCtctc                                 >  2:3266714/1‑150 (MQ=255)
                                                                                                                       gCTCTGCAGCACCCATAAAGCCGAGATTTCCCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCaa                               <  2:2559948/150‑1 (MQ=255)
                                                                                                                             cagcaTCCATAAAGCCGAGATTTCCCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGc                         <  2:3132486/150‑1 (MQ=255)
                                                                                                                             cagcaTCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGc                         >  1:36081/1‑150 (MQ=255)
                                                                                                                              agcaTCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCa                        >  2:3791827/1‑150 (MQ=255)
                                                                                                                               gcaTCGATAAGGCCGAGATTTCCCCCCTC‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCAt                       <  1:28356/150‑1 (MQ=255)
                                                                                                                                caTCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACTCGCGCTCTCAACCTTGCATa                      >  2:415507/1‑150 (MQ=255)
                                                                                                                                caTCCATAAAGCCGAGATTCACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATa                      <  1:629168/150‑1 (MQ=255)
                                                                                                                                  tCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAg                    >  2:2596797/1‑150 (MQ=255)
                                                                                                                                  tCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAg                    <  1:2161326/150‑1 (MQ=255)
                                                                                                                                   ccATAAAGCCGAGATTTCCCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGc                   <  1:735539/150‑1 (MQ=255)
                                                                                                                                   ccATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGc                   <  1:4180120/150‑1 (MQ=255)
                                                                                                                                   ccATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGc                   >  1:2148252/1‑150 (MQ=255)
                                                                                                                                     aTAAGGCCGAGTTTTCCCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGccc                 <  1:3278054/150‑1 (MQ=255)
                                                                                                                                     aTAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGccc                 >  1:4242964/1‑150 (MQ=255)
                                                                                                                                      tAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCa                >  2:4224071/1‑150 (MQ=255)
                                                                                                                                       aaaGCCGAGATTTCCCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACCCCTGACGCGCGCTCTCAACCTTGCATAAGCCCAg               <  2:1226772/150‑1 (MQ=255)
                                                                                                                                       aaaGCCGAGATTTCCCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAg               <  1:2384472/150‑1 (MQ=255)
                                                                                                                                         aGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGc             >  1:3704934/1‑150 (MQ=255)
                                                                                                                                          cccGATATTTCCCCCCCC‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCg            <  2:1498849/149‑1 (MQ=255)
                                                                                                                                          cccGAGTTTTCCCCCCGC‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCg            <  2:3856423/149‑1 (MQ=255)
                                                                                                                                            cGAGATTTCCCCCCGG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGAt          <  2:294943/150‑1 (MQ=255)
                                                                                                                                             gagaTTTCCCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGAtt         <  2:765522/150‑1 (MQ=255)
                                                                                                                                             gagaTTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGAtt         >  1:2958368/1‑150 (MQ=255)
                                                                                                                                             gagaTTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGAtt         >  2:2970125/1‑150 (MQ=255)
                                                                                                                                                aTTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATc      >  2:2374388/1‑150 (MQ=255)
                                                                                                                                                 tttCCCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAGCACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCa     <  2:4077638/150‑1 (MQ=255)
                                                                                                                                                  ttACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCaa    <  1:3171591/150‑1 (MQ=255)
                                                                                                                                                  ttACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCaa    >  2:2669452/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
CTGCATACCAGCCAGCATCAACGCCGCACTGTGCTTGCGGCTACGCTCATAGCGACGCAGATAAATGTACTGCCCGATGTCTTTCCCCTGACGATGCAACCGTTTCAGTTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTG‑CCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAA  >  AP012306/2557923‑2558220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑