Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,527,3680AC29.1% 986.4 / 89.4 420L140F (TTA→TTC) rhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
Reads supporting (aligned to +/- strand):  ref base A (248/49);  new base C (0/122);  total (248/172)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 512 total aligned reads displayed.

CGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTG  >  AP012306/3527219‑3527517
                                                                                                                                                      |                                                                                                                                                     
cGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTa                                                                                                                                                       <  2:3307005/150‑1 (MQ=255)
 gATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTAc                                                                                                                                                      <  2:2653282/150‑1 (MQ=255)
  aTTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTAcc                                                                                                                                                     >  1:1071453/1‑150 (MQ=255)
  aTTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTAcc                                                                                                                                                     >  2:745716/1‑150 (MQ=255)
  aTTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTAcc                                                                                                                                                     >  1:3397221/1‑150 (MQ=255)
  aTTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTAcc                                                                                                                                                     >  1:491217/1‑150 (MQ=255)
  aTTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTAcc                                                                                                                                                     >  1:3058148/1‑150 (MQ=255)
    ttCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCt                                                                                                                                                   >  2:3412684/1‑150 (MQ=255)
     tCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTc                                                                                                                                                  <  2:4006890/150‑1 (MQ=255)
     tCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTc                                                                                                                                                  <  1:2276638/150‑1 (MQ=255)
      cAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCg                                                                                                                                                 <  1:3626801/150‑1 (MQ=255)
          ttCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCg                                                                                                                                             >  1:3148291/1‑150 (MQ=255)
            ctcGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGcc                                                                                                                                           <  1:2188565/150‑1 (MQ=255)
            ctcGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGcc                                                                                                                                           >  1:541475/1‑150 (MQ=255)
            ctcGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGcc                                                                                                                                           <  1:79807/150‑1 (MQ=255)
                      tGAATCAGTTGCTGCCACAAGAGCTGGAGGGGCAGTATCCGTCTCACGGGCGCGTTACCCACAGCGTATTGCAGCGGGTGCGACAGCTGGTTGCACAGAGGAAACAGAAGGAGGGGA‑AAAATGATTTCCCCTCGACCGCCAGTCGCgaga                                                                                                                                 <  2:1918616/150‑1 (MQ=255)
                       gaaccgtttGCTGCCACAGGAGCTGGAGGGGCTGTACCCTTCCCACTGGCTCGTTAACCACAGGGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGAAACAGGAGGAAGGGG‑AAAATGTTTTCCCCTCGACCGCCAGTCGCGAGAt                                                                                                                                <  2:4209909/143‑1 (MQ=255)
                         aTCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCt                                                                                                                              >  1:658079/1‑150 (MQ=255)
                          tcattgcctgcCAAAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCtt                                                                                                                             <  2:1355968/143‑1 (MQ=255)
                              ttGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGttt                                                                                                                         <  1:2593474/150‑1 (MQ=255)
                              ttGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGttt                                                                                                                         >  1:3957869/1‑150 (MQ=255)
                              gtgctgcCACAAGAGCGGGATGGGCAGTATCCGTCTCACGGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCGGGTTGCACAGATGGAACAGCAGGAAGGGA‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGttt                                                                                                                         <  2:1697352/149‑1 (MQ=255)
                                      aaaaGAGCTGGATGGGCAGTACCCGTCCCACTGGCGCGTAACCCACAGCGTTTTGCAGCAGTTGCGACAGCTGGTTCCACAGATGGAACAGCAGGAAGGGA‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAAtt                                                                                                                 <  2:215750/148‑1 (MQ=255)
                                       caAGGGCGGGATGGGCAGTCCCCGTCTCACTGGCGCGTTACCCACAGCGTTTTGCAGCGGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAGGGGA‑AAAATGTTTTCCCCCCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTa                                                                                                                <  2:429874/150‑1 (MQ=255)
                                          gagCTGGATGGGCAGTATCCGTCTCACGGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGA‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTg                                                                                                             <  2:2339330/150‑1 (MQ=255)
                                           ggCTGGAGGGGCAGTATCCGTCTCACTGGCGCTTTACCCACAGCTTATTGCAGCAGGTGCGACAGCTGTTTGCACAGAGGGAACAGCGGGAAGGGA‑AAAATGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGc                                                                                                            <  2:2765339/149‑1 (MQ=255)
                                             gtGGAGGGGAAGTTCCCGTCCCAGGGGCGGTTTAACCACAGTGTATTGCGGGGGGTGCGACAGGGTGTTGCACAGATGGAACAGC‑GGAAGGGGAAAAATTTTTCCCCCTGAACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGctc                                                                                                          <  2:4439925/149‑1 (MQ=255)
                                             cTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGctc                                                                                                          >  1:3547553/1‑150 (MQ=255)
                                              tGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGctct                                                                                                         >  2:1330700/1‑150 (MQ=255)
                                              tGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGctct                                                                                                         >  2:2917792/1‑150 (MQ=255)
                                              tGGAGGGGCAGTACCCGTCCCCCGGGCGCGTTAACCACGGCGTATTGCAGCGGGGGCGACAGCTGGTGGCACAGAGGAAACAGCAGGAAGGGA‑AAAATGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGctct                                                                                                         <  2:932562/150‑1 (MQ=255)
                                                gATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtg                                                                                                       >  1:4083034/1‑150 (MQ=255)
                                                   gggCAGTATCCGTTTCACGGGGGCGTAAACCACGGCGTATTGAAGCAGGGGGGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtgctg                                                                                                    <  2:1537368/150‑1 (MQ=255)
                                                   gggCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtgctg                                                                                                    >  2:4112814/1‑150 (MQ=255)
                                                    ggCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtgctgc                                                                                                   <  1:1433922/150‑1 (MQ=255)
                                                     gCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCg                                                                                                  >  2:1979228/1‑150 (MQ=255)
                                                     gCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCg                                                                                                  >  2:785843/1‑150 (MQ=255)
                                                     gCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCGGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCGCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCg                                                                                                  <  2:3530942/150‑1 (MQ=255)
                                                       aGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTa                                                                                                >  1:3332673/1‑150 (MQ=255)
                                                       aGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTa                                                                                                >  2:1437812/1‑150 (MQ=255)
                                                       aGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTa                                                                                                >  1:1016819/1‑150 (MQ=255)
                                                       aGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTa                                                                                                >  1:4433244/1‑150 (MQ=255)
                                                       aGTATCCGTCCCACTGGCGCGTTAACCACAGCGTTTTGCAGCGGGTGCGACGGCTGGTTGCAGAGAGGGAACAGCAGGAAGGGA‑AAAATGTTTTCCCCTCGCCCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTa                                                                                                <  2:106955/150‑1 (MQ=255)
                                                          aTCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTaaaa                                                                                             >  1:2746859/1‑150 (MQ=255)
                                                              gTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAg                                                                                         >  1:4321438/1‑150 (MQ=255)
                                                                   ccTGGCGCTTTAACAAGAGTGTATGGCGGCGGGTGCAAAGGTGGGTTACAAATAGGAAAAAGCAGAAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGc                                                                                    <  2:573540/149‑1 (MQ=255)
                                                                      ggCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGAGGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCCCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAgg                                                                                 <  1:1507945/150‑1 (MQ=255)
                                                                      ggCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGATGCACAGATGGAACAGCGGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAgg                                                                                 <  2:3791214/150‑1 (MQ=255)
                                                                         gcgTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGA‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGgaga                                                                              <  2:4056067/150‑1 (MQ=255)
                                                                          cgTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGaa                                                                             >  1:114878/1‑150 (MQ=255)
                                                                           gTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAAc                                                                            >  1:2352418/1‑150 (MQ=255)
                                                                              aaCCACAGCGTATGGCAGCGGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAGGGGG‑AAAATGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTg                                                                         <  2:842641/150‑1 (MQ=255)
                                                                               aCCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTgg                                                                        >  1:195661/1‑150 (MQ=255)
                                                                                 cacaGCGTATGGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGaa                                                                      <  1:1352897/150‑1 (MQ=255)
                                                                                    aGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAAc                                                                   >  2:2047509/1‑150 (MQ=255)
                                                                                      cgtttggcagcaGGTGCGACAGTTGGTTGCACAGAGGAAAAAGCAGAAGGGGA‑AAAATAATTTCCCCCCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAg                                                                 <  2:1860278/144‑1 (MQ=255)
                                                                                      cGTATGGCGGCGGGGGCGACAGCTGGTTGCACAGATGAACCAGCGGGAAGGGA‑AAAATGATTTCCCCTCAACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAg                                                                 <  2:3100402/150‑1 (MQ=255)
                                                                                       gTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAgc                                                                >  2:4401639/1‑150 (MQ=255)
                                                                                         aTTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAgcgc                                                              >  2:391501/1‑150 (MQ=255)
                                                                                         aTTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAgcgc                                                              >  1:1649922/1‑150 (MQ=255)
                                                                                         aTTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAgcgc                                                              >  2:674293/1‑150 (MQ=255)
                                                                                           tGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGA‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCAt                                                            <  2:2256773/150‑1 (MQ=255)
                                                                                            ccacgagGGGCGAAAGGTGGTTGCACAGATGGAACAGCAGGAAGGGA‑AAATGGATTTCCCCCCGGCCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATc                                                           <  2:1775103/145‑1 (MQ=255)
                                                                                             cagcagGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCa                                                          <  1:3749353/150‑1 (MQ=255)
                                                                                             cagcagGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCa                                                          >  1:1812429/1‑150 (MQ=255)
                                                                                             cagcagGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCa                                                          >  1:865660/1‑150 (MQ=255)
                                                                                              agcagGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAc                                                         >  1:2929808/1‑150 (MQ=255)
                                                                                                 agGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGtc                                                      <  1:2476277/150‑1 (MQ=255)
                                                                                                    tGCGACAGCTGGTCGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCCCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCa                                                   <  1:3476413/150‑1 (MQ=255)
                                                                                                       gACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGA‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACt                                                <  2:3044219/150‑1 (MQ=255)
                                                                                                       gACAGCTGGTTGCAAAGATGGAACAGCAGGAAGGGG‑AAAATGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACt                                                <  2:2997589/150‑1 (MQ=255)
                                                                                                        aCAGCTGGTTGCACAGAGGAAACAGCAGGAGGGGA‑AAAATGTTTTCCCCCCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACtt                                               <  2:2096076/150‑1 (MQ=255)
                                                                                                         cggtgggTCGCACAGAGGGAACAGCAGGAAGGGA‑AAAATGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTg                                              <  1:3286839/145‑1 (MQ=255)
                                                                                                          aGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGc                                             <  1:1287897/150‑1 (MQ=255)
                                                                                                          aGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGc                                             >  1:3205039/1‑150 (MQ=255)
                                                                                                          aGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGc                                             >  2:521089/1‑150 (MQ=255)
                                                                                                             tGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTc                                          >  1:4195305/1‑150 (MQ=255)
                                                                                                             tGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTc                                          >  2:3578686/1‑150 (MQ=255)
                                                                                                              ggTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTct                                         >  1:2407411/1‑150 (MQ=255)
                                                                                                               gTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctg                                        >  2:3467349/1‑150 (MQ=255)
                                                                                                                tgacgcaGAGGCAACAGCAGGAAGGGT‑AAAAGCATTGCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctgg                                       <  2:4385699/145‑1 (MQ=255)
                                                                                                                 tGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggc                                      <  1:3839849/150‑1 (MQ=255)
                                                                                                                 tGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggc                                      >  1:1729197/1‑150 (MQ=255)
                                                                                                                     caGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcctgg                                  >  1:2789333/1‑150 (MQ=255)
                                                                                                                      aGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcctggc                                 >  2:2538223/1‑150 (MQ=255)
                                                                                                                            aaCAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTggagg                           >  2:1650092/1‑150 (MQ=255)
                                                                                                                               agcagGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCCCGTCTCAACTTGCTTCTGCCCTGGCTGGAGGAcc                        >  2:113492/1‑150 (MQ=255)
                                                                                                                                 cgggAAGGGA‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAt                      <  2:2368582/148‑1 (MQ=255)
                                                                                                                                 cagGAAGGGG‑AAAATGATTTCCCCTCCACCGCCAGTCGCGAGATCTTGTTTATGCAATTCCTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAt                      <  2:3669412/150‑1 (MQ=255)
                                                                                                                                  aggaggGGA‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAtt                     <  2:3176224/150‑1 (MQ=255)
                                                                                                                                  agGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAtt                     >  2:3723637/1‑150 (MQ=255)
                                                                                                                                   ggAAGGGG‑AAAATGATTTCCCCCCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAttt                    <  2:1577928/150‑1 (MQ=255)
                                                                                                                                     aaGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTg                  <  1:2900897/150‑1 (MQ=255)
                                                                                                                                      aGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGc                 >  2:2862963/1‑150 (MQ=255)
                                                                                                                                         gg‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGa              >  1:763145/1‑150 (MQ=255)
                                                                                                                                          g‑AAAATGATTTACCCTCAACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGAt             >  2:1226581/1‑150 (MQ=255)
                                                                                                                                            aaaaTGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATg            >  2:2446828/1‑150 (MQ=255)
                                                                                                                                             aaaTGATTTCCCCTGAACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGa           <  1:2063776/150‑1 (MQ=255)
                                                                                                                                                     tACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAAtt   >  2:4244313/1‑150 (MQ=255)
                                                                                                                                                     tACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAAtt   >  1:997040/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTG  >  AP012306/3527219‑3527517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑