BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	1,437,314	C→A	P12T (CCG→ACG)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	1,437,314	0	C	A	100.0%	1397.0 / NA	337	P12T (CCG→ACG)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (178/159); total (178/159)

Only 100 of 340 total aligned reads displayed.

ACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACC  >  AP012306/1437168‑1437460

aCCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAATTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGa                                                                                                                                                 >  1:3230948/1‑150 (MQ=255)

  cTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGaaa                                                                                                                                               >  1:4071981/1‑150 (MQ=255)

  cTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGaaa                                                                                                                                               <  1:606145/150‑1 (MQ=255)

         tAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAAt                                                                                                                                        <  2:3501579/150‑1 (MQ=255)

         tAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAAt                                                                                                                                        <  2:1677762/150‑1 (MQ=255)

          agagCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATc                                                                                                                                       <  2:1206174/150‑1 (MQ=255)

          agagCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATc                                                                                                                                       <  2:2111746/150‑1 (MQ=255)

             gCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGa                                                                                                                                    <  2:3071913/150‑1 (MQ=255)

              cGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGaa                                                                                                                                   >  1:3764500/1‑150 (MQ=255)

               gAGCCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAag                                                                                                                                  <  2:3662742/150‑1 (MQ=255)

                  gCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAgaga                                                                                                                               >  2:3125186/1‑150 (MQ=255)

                   caccaccacTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGAt                                                                                                                              <  1:2709432/150‑1 (MQ=255)

                   caccaccacTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGAt                                                                                                                              >  2:1995229/1‑150 (MQ=255)

                   caccaccacGTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCCCCATCGGAACGAAAACCGAATCTGAAGAGAt                                                                                                                              <  1:422258/150‑1 (MQ=255)

                     ccaccacTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGt                                                                                                                            <  1:301352/150‑1 (MQ=255)

                       accacTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTa                                                                                                                          >  2:2295350/1‑150 (MQ=255)

                         cacTTTCGTAATACCTGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGc                                                                                                                        <  2:3611739/150‑1 (MQ=255)

                          acTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCt                                                                                                                       >  2:2990154/1‑150 (MQ=255)

                          acTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCt                                                                                                                       <  2:3169964/150‑1 (MQ=255)

                          acTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCt                                                                                                                       <  1:2781743/150‑1 (MQ=255)

                           cTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTa                                                                                                                      >  1:962951/1‑150 (MQ=255)

                              tCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTaaaa                                                                                                                   <  2:1938329/150‑1 (MQ=255)

                              tCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTaaaa                                                                                                                   <  2:841226/150‑1 (MQ=255)

                                gTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATg                                                                                                                 >  2:897522/1‑150 (MQ=255)

                                    tACCGGATTCGCTTTCCTGCAGTGCGCCCAGAAAGCACATTTCTCGCACCCTTCTCAACTTAAAGACTAATACTGTCATGACAAAGACCTAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGGTAGCTAAAATGCTgg                                                                                                             >  2:431569/1‑150 (MQ=255)

                                        ggATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGc                                                                                                         <  1:4352734/150‑1 (MQ=255)

                                        ggATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGc                                                                                                         >  1:1534920/1‑150 (MQ=255)

                                          aTTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTg                                                                                                       <  2:271910/150‑1 (MQ=255)

                                           ttCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTgg                                                                                                      <  1:746628/150‑1 (MQ=255)

                                               cTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGaaaa                                                                                                                                              <  2:3904604/106‑1 (MQ=255)

                                               cTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGaaaa                                                                                                                                              >  1:3904604/1‑106 (MQ=255)

                                               cTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATg                                                                                                  >  1:2501085/1‑150 (MQ=255)

                                                tttCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATAAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGa                                                                                                 >  2:2366090/1‑150 (MQ=255)

                                                  tCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAAc                                                                                               <  1:2972820/150‑1 (MQ=255)

                                                   ccGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACg                                                                                              >  2:1572185/1‑150 (MQ=255)

                                                    cGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGt                                                                                             >  1:2318883/1‑150 (MQ=255)

                                                       cAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTAt                                                                                          >  1:3180005/1‑150 (MQ=255)

                                                       cAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTAt                                                                                          <  2:1511873/150‑1 (MQ=255)

                                                             gcCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCt                                                                                    <  1:2759235/150‑1 (MQ=255)

                                                              cccAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTg                                                                                   <  1:1813954/150‑1 (MQ=255)

                                                              cccAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTg                                                                                   >  1:857398/1‑150 (MQ=255)

                                                               ccAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGa                                                                                  >  1:888155/1‑150 (MQ=255)

                                                                cAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGaa                                                                                 >  1:3321443/1‑150 (MQ=255)

                                                                cAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGaa                                                                                 <  1:1755636/150‑1 (MQ=255)

                                                                  gAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACt                                                                               >  1:1385663/1‑150 (MQ=255)

                                                                  gAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACt                                                                               <  1:2082340/150‑1 (MQ=255)

                                                                   aaaGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACtt                                                                              >  1:1241398/1‑150 (MQ=255)

                                                                    aaGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTtc                                                                             >  2:650294/1‑150 (MQ=255)

                                                                        aaGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTtctctc                                                                         >  2:2503040/1‑150 (MQ=255)

                                                                         aGTTACTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCa                                                                        <  2:4402729/150‑1 (MQ=255)

                                                                          gTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCAt                                                                       <  1:103190/150‑1 (MQ=255)

                                                                                 ccATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACt                                                                >  2:1065473/1‑150 (MQ=255)

                                                                                   aTCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTAt                                                              <  2:3312046/150‑1 (MQ=255)

                                                                                   aTCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTAt                                                              >  1:2864170/1‑150 (MQ=255)

                                                                                    tCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATg                                                             >  1:2155305/1‑150 (MQ=255)

                                                                                    tCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATg                                                             >  2:4215990/1‑150 (MQ=255)

                                                                                     ccTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGc                                                            <  2:3241643/150‑1 (MQ=255)

                                                                                     ccTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGc                                                            <  1:1309331/150‑1 (MQ=255)

                                                                                        tctcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGa                                                         >  1:2828536/1‑150 (MQ=255)

                                                                                          tcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAac                                                       >  1:3223122/1‑150 (MQ=255)

                                                                                             aCTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACg                                                    <  1:3834399/150‑1 (MQ=255)

                                                                                                   agactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAATGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAgc                                              >  1:1350608/1‑150 (MQ=255)

                                                                                                    gactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAgcg                                             >  1:1977665/1‑150 (MQ=255)

                                                                                                      ctaagactGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCa                                           <  2:1843375/150‑1 (MQ=255)

                                                                                                      ctaagactGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCa                                           >  1:3476298/1‑150 (MQ=255)

                                                                                                       taagactGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCAt                                          <  2:2144716/150‑1 (MQ=255)

                                                                                                       taagactGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCAt                                          >  1:2013304/1‑150 (MQ=255)

                                                                                                        aagactGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCAtt                                         >  1:2585434/1‑150 (MQ=255)

                                                                                                         agactGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTc                                        >  2:3579003/1‑150 (MQ=255)

                                                                                                         agactGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTc                                        >  2:2907149/1‑150 (MQ=255)

                                                                                                         agactGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTc                                        <  2:2797220/150‑1 (MQ=255)

                                                                                                           actGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAAATGTTACCTAAAATGCTAGACGCTGGCATGACCGGTATGAGGCTGAATTTCTCTCATTGTGACTATGCAGAACACCGTCAGCGCATTCAg                                      >  2:3353525/1‑150 (MQ=255)

                                                                                                            ctGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGa                                     >  2:2779530/1‑150 (MQ=255)

                                                                                                             tGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGaa                                    <  1:4151581/150‑1 (MQ=255)

                                                                                                             tGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGaa                                    >  1:1695674/1‑150 (MQ=255)

                                                                                                              gTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAAt                                   >  1:2462332/1‑150 (MQ=255)

                                                                                                               tCATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATc                                  >  1:1183599/1‑150 (MQ=255)

                                                                                                                cATGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCt                                 <  1:1199451/150‑1 (MQ=255)

                                                                                                                 aTGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTg                                <  2:1114497/150‑1 (MQ=255)

                                                                                                                  tGAAAAAGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTgc                               >  2:1297316/1‑150 (MQ=255)

                                                                                                                    aaaaaGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTgcgc                             >  2:1784515/1‑150 (MQ=255)

                                                                                                                       aaGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAAc                          <  1:395486/150‑1 (MQ=255)

                                                                                                                       aaGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAAc                          <  1:2273470/150‑1 (MQ=255)

                                                                                                                        aGACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGATATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACg                         <  2:4039826/150‑1 (MQ=255)

                                                                                                                         gACCAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGt                        >  1:3410339/1‑150 (MQ=255)

                                                                                                                            cAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGtgat                     <  1:696429/150‑1 (MQ=255)

                                                                                                                            cAAAATTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGtgat                     >  1:3634024/1‑150 (MQ=255)

                                                                                                                              aaaTTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGtgatga                   >  1:4010312/1‑150 (MQ=255)

                                                                                                                              aaaTTGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGtgatga                   >  2:713564/1‑150 (MQ=255)

                                                                                                                                 ttGTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCa                <  2:557377/150‑1 (MQ=255)

                                                                                                                                   gTTTGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCaaa              >  1:3594652/1‑150 (MQ=255)

                                                                                                                                    tttGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCaaaa             >  1:2379425/1‑150 (MQ=255)

                                                                                                                                     ttGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAAc            >  1:261220/1‑150 (MQ=255)

                                                                                                                                      tGCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACt           <  2:3851612/150‑1 (MQ=255)

                                                                                                                                       gCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTg          <  2:4139686/150‑1 (MQ=255)

                                                                                                                                       gCACCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTg          <  1:458789/150‑1 (MQ=255)

                                                                                                                                         aCCATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGt        <  2:3712972/150‑1 (MQ=255)

                                                                                                                                           cATCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTaa      <  2:1385703/150‑1 (MQ=255)

                                                                                                                                            aTCGGAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTaaa     >  2:2673584/1‑150 (MQ=255)

                                                                                                                                               ggAACGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAAcc  >  1:2561009/1‑150 (MQ=255)

ACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACC  >  AP012306/1437168‑1437460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`