Predicted mutation
evidence seq id position mutation annotation gene description
RA AP012306 1,476,895 A→T F81I (TTC→ATC)  ihfA ← integration host factor (IHF), DNA‑binding protein, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,476,8950AT100.0% 1222.8 / NA 306F81I (TTC→ATC) ihfAintegration host factor (IHF), DNA‑binding protein, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (158/148);  total (158/148)

Only 100 of 309 total aligned reads displayed.

GCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCT  >  AP012306/1476748‑1477040
                                                                                                                                                   |                                                                                                                                                 
gctgAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATgg                                                                                                                                                 <  1:660453/150‑1 (MQ=255)
 ctgAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGt                                                                                                                                                >  2:1102238/1‑150 (MQ=255)
     aGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTcacc                                                                                                                                            >  1:3629245/1‑150 (MQ=255)
       tgtCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTcaccac                                                                                                                                          <  2:223949/150‑1 (MQ=255)
       tgtCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTcaccac                                                                                                                                          <  1:4208436/150‑1 (MQ=255)
        gtCATGGCGTTGATTTTACGGTGACTGTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCAcg                                                                                                                                         <  2:1510036/150‑1 (MQ=255)
        gtCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCAcg                                                                                                                                         >  1:2108368/1‑150 (MQ=255)
         tCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCAcgc                                                                                                                                        >  1:1301847/1‑150 (MQ=255)
           aTGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACgcgc                                                                                                                                      >  1:2129283/1‑150 (MQ=255)
             ggCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCg                                                                                                                                    <  2:2861745/150‑1 (MQ=255)
             ggCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCg                                                                                                                                    >  1:3578505/1‑150 (MQ=255)
                gTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGc                                                                                                                                 <  2:2178204/150‑1 (MQ=255)
                 ttGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCt                                                                                                                                <  2:853540/150‑1 (MQ=255)
                    aTTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTa                                                                                                                             >  1:2006577/1‑150 (MQ=255)
                    aTTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTa                                                                                                                             <  2:1065127/150‑1 (MQ=255)
                     ttttACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTaa                                                                                                                            >  1:3217448/1‑150 (MQ=255)
                     ttttACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTaa                                                                                                                            >  2:3685217/1‑150 (MQ=255)
                            gTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAat                                                                                                                     >  2:3916821/1‑150 (MQ=255)
                                ctctTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCGCCACGCGCCGTGCTGTAATGGGAATATcc                                                                                                                 <  1:3491703/150‑1 (MQ=255)
                                  ctTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTTGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTc                                                                                                               >  1:3687153/1‑150 (MQ=255)
                                  ctTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTc                                                                                                               <  1:1191894/150‑1 (MQ=255)
                                   ttCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCg                                                                                                              >  1:4176795/1‑150 (MQ=255)
                                      gACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGccc                                                                                                           >  2:621787/1‑150 (MQ=255)
                                       aCAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCg                                                                                                          <  2:1177949/150‑1 (MQ=255)
                                         aGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGtt                                                                                                        >  2:3846202/1‑150 (MQ=255)
                                          gTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGttt                                                                                                       >  1:2186087/1‑150 (MQ=255)
                                          gTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGttt                                                                                                       >  2:4098915/1‑150 (MQ=255)
                                            gaaaagaaaaAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTc                                                                                                     <  2:400766/150‑1 (MQ=255)
                                             aaaagaaaaAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCg                                                                                                    >  1:3136910/1‑150 (MQ=255)
                                                agaaaaAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGt                                                                                                 >  2:3581656/1‑150 (MQ=255)
                                                 gaaaaAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGtt                                                                                                >  2:2500930/1‑150 (MQ=255)
                                                  aaaaaaGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAGGCGTTTCCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGCCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTa                                                                                               <  2:1045564/150‑1 (MQ=255)
                                                   aaaaaGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTAc                                                                                              <  2:2075035/150‑1 (MQ=255)
                                                    aaaaGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACg                                                                                             >  2:1286141/1‑150 (MQ=255)
                                                        ggCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTccc                                                                                         <  2:1717740/150‑1 (MQ=255)
                                                           cGCAGAGCGGTCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCggg                                                                                      >  2:1345338/1‑150 (MQ=255)
                                                           cGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCggg                                                                                      >  1:585214/1‑150 (MQ=255)
                                                            gCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGc                                                                                     <  1:1964355/150‑1 (MQ=255)
                                                            gCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGc                                                                                     >  2:2942610/1‑150 (MQ=255)
                                                              agagCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGt                                                                                   <  2:1076058/150‑1 (MQ=255)
                                                               gagCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGtt                                                                                  <  1:3951477/150‑1 (MQ=255)
                                                                 gCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGa                                                                                >  1:1751837/1‑150 (MQ=255)
                                                                    gCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTc                                                                             >  1:540578/1‑150 (MQ=255)
                                                                     ccTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCt                                                                            >  2:3225763/1‑150 (MQ=255)
                                                                      cTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCtt                                                                           <  1:2874047/150‑1 (MQ=255)
                                                                         tttAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATc                                                                        >  2:2789402/1‑150 (MQ=255)
                                                                          ttagttagATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCa                                                                       >  2:722073/1‑150 (MQ=255)
                                                                          ttagttagATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCa                                                                       >  1:4144685/1‑150 (MQ=255)
                                                                           tagttagATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCAc                                                                      >  1:3780921/1‑150 (MQ=255)
                                                                           tagttagATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCAc                                                                      >  1:1874684/1‑150 (MQ=255)
                                                                            agttagATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACg                                                                     <  1:2399985/150‑1 (MQ=255)
                                                                              ttagATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCa                                                                   >  1:2626678/1‑150 (MQ=255)
                                                                               tagATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAg                                                                  >  2:4281761/1‑150 (MQ=255)
                                                                               tagATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAg                                                                  >  2:3996902/1‑150 (MQ=255)
                                                                                 gATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGAt                                                                <  1:2241487/150‑1 (MQ=255)
                                                                                 gATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGAt                                                                >  1:964959/1‑150 (MQ=255)
                                                                                    cAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGa                                                             >  1:2552469/1‑150 (MQ=255)
                                                                                     aGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGaa                                                            <  2:2553292/150‑1 (MQ=255)
                                                                                      gATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAg                                                           >  1:2060835/1‑150 (MQ=255)
                                                                                        ttACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGtt                                                         <  1:4374396/150‑1 (MQ=255)
                                                                                        ttACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGtt                                                         <  2:3095119/150‑1 (MQ=255)
                                                                                           cTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTAcc                                                      >  1:1012004/1‑150 (MQ=255)
                                                                                            tCGTCTTTGGGCTAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCa                                                     <  2:959688/150‑1 (MQ=255)
                                                                                             cGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCaa                                                    >  1:4102717/1‑150 (MQ=255)
                                                                                              gTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCaaa                                                   <  2:2380504/150‑1 (MQ=255)
                                                                                              gTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCaaa                                                   <  2:1451809/150‑1 (MQ=255)
                                                                                               tCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCaaaa                                                  >  2:1841430/1‑150 (MQ=255)
                                                                                               tCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCaaaa                                                  >  1:2781159/1‑150 (MQ=255)
                                                                                                cTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAAc                                                 <  2:67372/150‑1 (MQ=255)
                                                                                                cTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAAc                                                 >  1:4019748/1‑150 (MQ=255)
                                                                                                    gggCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCaga                                             >  2:2610119/1‑150 (MQ=255)
                                                                                                     ggCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCagag                                            >  2:569642/1‑150 (MQ=255)
                                                                                                       cGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCagagag                                          >  2:4127609/1‑150 (MQ=255)
                                                                                                         aaGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGtt                                        <  1:1500977/150‑1 (MQ=255)
                                                                                                         aaGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGtt                                        <  2:1613460/150‑1 (MQ=255)
                                                                                                          aGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGttt                                       >  2:1952543/1‑150 (MQ=255)
                                                                                                            cGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCa                                     >  1:3033563/1‑150 (MQ=255)
                                                                                                                  cGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGtt                               >  1:1086078/1‑150 (MQ=255)
                                                                                                                  cGACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGtt                               <  1:2955256/150‑1 (MQ=255)
                                                                                                                   gACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTc                              <  1:4309342/150‑1 (MQ=255)
                                                                                                                   gACCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTc                              <  2:777036/150‑1 (MQ=255)
                                                                                                                    aCCCGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCg                             <  1:1632089/150‑1 (MQ=255)
                                                                                                                      ccGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGcc                           >  2:2111211/1‑150 (MQ=255)
                                                                                                                      ccGGCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGcc                           <  1:1244777/150‑1 (MQ=255)
                                                                                                                        ggCTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGt                         <  2:3278150/150‑1 (MQ=255)
                                                                                                                          cTTTTTAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGttt                       <  2:234137/150‑1 (MQ=255)
                                                                                                                           tttttAACTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGtttt                      <  2:1784123/150‑1 (MQ=255)
                                                                                                                                aaCTTCTCCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCGCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCCCCTGTTCGCCGTTTTCCaga                 <  2:3016824/150‑1 (MQ=255)
                                                                                                                                 aCTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCagag                <  1:871406/150‑1 (MQ=255)
                                                                                                                                 aCTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCagag                <  2:804023/150‑1 (MQ=255)
                                                                                                                                  cTTCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAgc               <  1:2682452/150‑1 (MQ=255)
                                                                                                                                   ttCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAgcg              >  1:741985/1‑150 (MQ=255)
                                                                                                                                   ttCTGCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAgcg              >  2:1140438/1‑150 (MQ=255)
                                                                                                                                       gCCCGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGAc          <  1:1444954/150‑1 (MQ=255)
                                                                                                                                        cccGGGTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACg         <  2:283317/150‑1 (MQ=255)
                                                                                                                                           gggTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGAt      >  2:499693/1‑150 (MQ=255)
                                                                                                                                           gggTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGAt      >  1:3862477/1‑150 (MQ=255)
                                                                                                                                            ggTCTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGAtc     >  1:1819252/1‑150 (MQ=255)
                                                                                                                                               cTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCTGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCACAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATctct  <  2:3411376/150‑1 (MQ=255)
                                                                                                                                               cTGATGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATctct  <  2:4327693/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCCTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCT  >  AP012306/1476748‑1477040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑