BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	1,587,473	G→A	Q37* (CAG→TAG)	cspC ←	stress protein, member of the CspA‑family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	1,587,473	0	G	A	100.0%	1715.2 / NA	418	Q37* (CAG→TAG)	cspC	stress protein, member of the CspA‑family
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (248/170); total (248/170)

Only 100 of 424 total aligned reads displayed.

TATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGC  >  AP012306/1587326‑1587619

 ataGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGAt                                                                                                                                                 >  1:1352899/1‑150 (MQ=255)

  taGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATa                                                                                                                                                <  1:3691393/150‑1 (MQ=255)

   aGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAg                                                                                                                                               >  1:2837431/1‑150 (MQ=255)

       agaTTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGga                                                                                                                                           <  1:483956/150‑1 (MQ=255)

       agaTTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGga                                                                                                                                           >  1:1017376/1‑150 (MQ=255)

        gaTTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGgag                                                                                                                                          >  2:3993594/1‑150 (MQ=255)

           tGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAg                                                                                                                                       >  2:3531820/1‑150 (MQ=255)

            gAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAgt                                                                                                                                      <  1:661532/150‑1 (MQ=255)

             aaGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAgtg                                                                                                                                     >  2:4246565/1‑150 (MQ=255)

              aGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAgtgt                                                                                                                                    >  1:4406052/1‑150 (MQ=255)

               gCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGCAGTGTa                                                                                                                                   >  2:3140413/1‑150 (MQ=255)

               gCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTa                                                                                                                                   >  1:1224111/1‑150 (MQ=255)

               gCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTa                                                                                                                                   <  1:3031219/150‑1 (MQ=255)

                cGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTAc                                                                                                                                  >  1:4084664/1‑150 (MQ=255)

                 gTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACg                                                                                                                                 >  1:3370634/1‑150 (MQ=255)

                  tATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGa                                                                                                                                <  1:3455888/150‑1 (MQ=255)

                     tCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAaca                                                                                                                             >  2:876147/1‑150 (MQ=255)

                      cacaCTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAacac                                                                                                                            >  1:75416/1‑150 (MQ=255)

                       acacTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAAcaca                                                                                                                           >  1:3963782/1‑150 (MQ=255)

                       acacTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAAcaca                                                                                                                           >  2:3630786/1‑150 (MQ=255)

                       acacTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAAcaca                                                                                                                           >  1:1261134/1‑150 (MQ=255)

                         acTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATc                                                                                                                         >  1:2541103/1‑150 (MQ=255)

                         acTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATc                                                                                                                         >  2:2840536/1‑150 (MQ=255)

                          cTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCt                                                                                                                        <  2:234546/150‑1 (MQ=255)

                           ttCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCtt                                                                                                                       >  1:40304/1‑150 (MQ=255)

                           ttCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCtt                                                                                                                       >  2:2568477/1‑150 (MQ=255)

                            tCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCttt                                                                                                                      >  2:1624232/1‑150 (MQ=255)

                             cAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTtg                                                                                                                     <  2:2206687/150‑1 (MQ=255)

                               gATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTtgct                                                                                                                   <  2:1737242/150‑1 (MQ=255)

                               gATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTtgct                                                                                                                   >  2:649042/1‑150 (MQ=255)

                               gATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGCACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTtgct                                                                                                                   <  2:3189452/150‑1 (MQ=255)

                                aTCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTtgctg                                                                                                                  <  2:3292741/150‑1 (MQ=255)

                                       gATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAg                                                                                                           >  2:2789793/1‑150 (MQ=255)

                                       gATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAg                                                                                                           <  2:1076125/150‑1 (MQ=255)

                                        aTTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc                                                                                                          <  2:1559762/150‑1 (MQ=255)

                                         ttCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGcc                                                                                                         <  1:849377/150‑1 (MQ=255)

                                             aTCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAg                                                                                                     >  2:2963584/1‑150 (MQ=255)

                                                   tAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGa                                                                                               >  2:262889/1‑150 (MQ=255)

                                                   tAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGa                                                                                               >  2:119090/1‑150 (MQ=255)

                                                      cTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGc                                                                                            <  2:2676192/150‑1 (MQ=255)

                                                      cTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGc                                                                                            >  2:3001412/1‑150 (MQ=255)

                                                      cTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGc                                                                                            <  1:1714083/150‑1 (MQ=255)

                                                       tGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGcc                                                                                           >  2:4365828/1‑150 (MQ=255)

                                                        gTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGGCCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCa                                                                                          <  1:990320/150‑1 (MQ=255)

                                                            cGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc                                                                                      <  1:2284472/150‑1 (MQ=255)

                                                            cGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc                                                                                      >  2:472084/1‑150 (MQ=255)

                                                               tAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCtt                                                                                   >  2:289188/1‑150 (MQ=255)

                                                                aaCAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCttt                                                                                  >  1:3263534/1‑150 (MQ=255)

                                                                 aCAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTa                                                                                 >  2:240233/1‑150 (MQ=255)

                                                                    gCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGAc                                                                              <  1:2525316/150‑1 (MQ=255)

                                                                        ccGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAAAACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGt                                                                          >  2:3337187/1‑150 (MQ=255)

                                                                         cGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGtt                                                                         <  2:1924792/150‑1 (MQ=255)

                                                                         cGGACCTTCCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGtt                                                                         <  2:4177076/150‑1 (MQ=255)

                                                                          ggACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTg                                                                        >  1:2655788/1‑150 (MQ=255)

                                                                           gACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGa                                                                       <  1:3620193/150‑1 (MQ=255)

                                                                            aCCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGaa                                                                      >  1:1787965/1‑150 (MQ=255)

                                                                            aCCTTTCTGGCCGTCCCGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGaa                                                                      >  1:3113313/1‑150 (MQ=255)

                                                                             ccTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAAc                                                                     <  1:2162842/150‑1 (MQ=255)

                                                                             ccTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAAc                                                                     <  1:3644364/150‑1 (MQ=255)

                                                                               tttCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCa                                                                   >  2:3046922/1‑150 (MQ=255)

                                                                                ttCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCAc                                                                  <  1:3519278/150‑1 (MQ=255)

                                                                                   tGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTa                                                               >  2:3733876/1‑150 (MQ=255)

                                                                                    ggCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTaa                                                              >  2:695646/1‑150 (MQ=255)

                                                                                       cGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCt                                                           >  2:228970/1‑150 (MQ=255)

                                                                                         tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGa                                                         >  1:2412898/1‑150 (MQ=255)

                                                                                          ccTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGAc                                                        <  1:2041342/150‑1 (MQ=255)

                                                                                          ccTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGAc                                                        >  1:2494608/1‑150 (MQ=255)

                                                                                           cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGAcc                                                       <  1:551002/150‑1 (MQ=255)

                                                                                               aTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTa                                                   <  2:773094/150‑1 (MQ=255)

                                                                                                 ttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAAt                                                 <  2:1409711/150‑1 (MQ=255)

                                                                                                     aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCttt                                             >  2:3932408/1‑150 (MQ=255)

                                                                                                     aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCttt                                             >  1:1937759/1‑150 (MQ=255)

                                                                                                     aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCttt                                             <  1:891930/150‑1 (MQ=255)

                                                                                                     aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCttt                                             <  1:413019/150‑1 (MQ=255)

                                                                                                      aCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTg                                            >  1:288187/1‑150 (MQ=255)

                                                                                                         cAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCa                                         >  1:1672200/1‑150 (MQ=255)

                                                                                                          aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt                                        <  2:1352336/150‑1 (MQ=255)

                                                                                                           aCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAtt                                       >  2:1946402/1‑150 (MQ=255)

                                                                                                           aCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAtt                                       <  1:2561256/150‑1 (MQ=255)

                                                                                                           aCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAtt                                       <  1:1165627/150‑1 (MQ=255)

                                                                                                             gTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTg                                     >  2:4402466/1‑150 (MQ=255)

                                                                                                             gTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTg                                     >  1:1568769/1‑150 (MQ=255)

                                                                                                              ttCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGa                                    >  2:3768231/1‑150 (MQ=255)

                                                                                                              ttCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGa                                    >  1:3214757/1‑150 (MQ=255)

                                                                                                               tCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAATTGaa                                   >  1:4442298/1‑150 (MQ=255)

                                                                                                                   aCCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAAt                               >  1:3886980/1‑150 (MQ=255)

                                                                                                                      ttCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTcc                            <  1:2725051/150‑1 (MQ=255)

                                                                                                                       tCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCt                           <  1:1475364/150‑1 (MQ=255)

                                                                                                                            cAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGAt                      >  2:1681703/1‑150 (MQ=255)

                                                                                                                            cAGAGTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGAt                      <  1:3811942/150‑1 (MQ=255)

                                                                                                                                gTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGtt                  >  2:570530/1‑150 (MQ=255)

                                                                                                                                gTTTTGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGtt                  >  1:4354650/1‑150 (MQ=255)

                                                                                                                                 ttttGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGttt                 >  2:1096899/1‑150 (MQ=255)

                                                                                                                                 ttttGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGttt                 <  1:610639/150‑1 (MQ=255)

                                                                                                                                 ttttGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGttt                 <  1:1084388/150‑1 (MQ=255)

                                                                                                                                  tttGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGtttt                <  2:1328940/150‑1 (MQ=255)

                                                                                                                                   ttGAAGCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTttc               <  2:2057716/150‑1 (MQ=255)

                                                                                                                                        gCCATTACCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGc          >  1:2954467/1‑150 (MQ=255)

                                                                                                                                              aCCCTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAg    <  2:1776191/150‑1 (MQ=255)

                                                                                                                                               cccTAGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAgg   <  1:1425614/150‑1 (MQ=255)

TATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGC  >  AP012306/1587326‑1587619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`