Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,758,9090TG30.6% 449.4 / 14.3 193G33G (GGT→GGG) agaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
Reads supporting (aligned to +/- strand):  ref base T (41/93);  new base G (59/0);  total (100/93)
Fisher's exact test for biased strand distribution p-value = 1.00e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 261 total aligned reads displayed.

AAGCGCAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGA  >  AP012306/2758760‑2759058
                                                                                                                                                      |                                                                                                                                                     
  gcgcAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGttt                                                                                                                                                     <  2:2118042/150‑1 (MQ=255)
  gcgcAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGttt                                                                                                                                                     <  2:113088/150‑1 (MQ=255)
    gcAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTa                                                                                                                                                   <  2:907388/150‑1 (MQ=255)
    gcAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTa                                                                                                                                                   >  1:1381235/1‑150 (MQ=255)
          gCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTggg                                                                                                                                             <  2:68205/150‑1 (MQ=255)
             gCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGa                                                                                                                                          <  2:2411656/150‑1 (MQ=255)
              cccGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGAt                                                                                                                                         <  1:881395/150‑1 (MQ=255)
               ccGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATg                                                                                                                                        >  1:3038174/1‑150 (MQ=255)
                   tGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGc                                                                                                                                    <  1:2026105/150‑1 (MQ=255)
                   tGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGCCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGGGAAGTTGc                                                                                                                                    >  2:2675975/1‑150 (MQ=255)
                     ccAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTTGGCGATGTTGCCg                                                                                                                                  >  1:2999364/1‑150 (MQ=255)
                      cAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTAACTGGGCGATGTTGCCGa                                                                                                                                 >  1:765172/1‑150 (MQ=255)
                       aGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGAt                                                                                                                                <  1:1167810/150‑1 (MQ=255)
                           aaaaTGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTg                                                                                                                            >  1:451592/1‑150 (MQ=255)
                           aaaaTGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTg                                                                                                                            <  2:4031159/150‑1 (MQ=255)
                             aaTGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGaa                                                                                                                          <  1:1220540/150‑1 (MQ=255)
                                 aagaagAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTCGGCGATGTTTCCGATGCTTaaaaaa                                                                                                                      >  2:1535935/1‑149 (MQ=255)
                                 aagaagAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGGTGTTGCCGATCCTGAAAAAg                                                                                                                      >  2:2818783/1‑150 (MQ=255)
                                   gaagaGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGAt                                                                                                                    <  1:3141324/150‑1 (MQ=255)
                                   gaagaGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGGGGTGTTGTCGATCCCGGAAAAAAt                                                                                                                    >  2:1989644/1‑150 (MQ=255)
                                   gaagaGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGAt                                                                                                                    >  2:3132586/1‑150 (MQ=255)
                                     agagGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGAttt                                                                                                                  >  1:3904182/1‑150 (MQ=255)
                                      gagGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTta                                                                                                                 <  2:2958842/150‑1 (MQ=255)
                                       agGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTtat                                                                                                                <  1:3467704/150‑1 (MQ=255)
                                         gACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTTCCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATaa                                                                                                              >  1:2420676/1‑150 (MQ=255)
                                          aCTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAg                                                                                                             >  1:1503886/1‑150 (MQ=255)
                                          aCTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGATGTTGCCCATCCTGAAAAAGATTTTTAAg                                                                                                             >  2:2629054/1‑150 (MQ=255)
                                            tACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGga                                                                                                           <  1:3081850/150‑1 (MQ=255)
                                            tACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGga                                                                                                           <  2:3609978/150‑1 (MQ=255)
                                            tACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGAA‑GGGTTTTCCCGGGCGCACTTCCCGACCCTTCAAAAGAATtttactgg                                                                                                           >  2:2353419/1‑146 (MQ=255)
                                             aCAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGgac                                                                                                          <  1:4012440/150‑1 (MQ=255)
                                             aCAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGgac                                                                                                          >  1:3560036/1‑150 (MQ=255)
                                               aGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGgacga                                                                                                        >  1:2765785/1‑150 (MQ=255)
                                                gCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCTCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGgacgac                                                                                                       <  2:53638/150‑1 (MQ=255)
                                                 cAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGG‑GGTTTTTTCTGGGCGATGTTGCCGGTCCTGAAAAAGATTTATAAAGAAGACa                                                                                                      >  2:3449801/1‑150 (MQ=255)
                                                  aaTGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACaa                                                                                                     >  1:1608916/1‑150 (MQ=255)
                                                   aTGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGATGTTGCCGGTCCTGAAAAAAATTTATAAGGACGACaaa                                                                                                    >  2:48383/1‑150 (MQ=255)
                                                    tGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAAc                                                                                                   <  1:1278132/150‑1 (MQ=255)
                                                    tGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTACTGGGCGATGTTGCCGGTACTTGAAAAGATTTTTAAAGAAGACAAAc                                                                                                   >  1:1932099/1‑150 (MQ=255)
                                                    tGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCCGATCCCGAAAAAGATTTATAAGGACGACAAAc                                                                                                   >  1:581015/1‑150 (MQ=255)
                                                      ggATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCg                                                                                                 <  1:2635001/150‑1 (MQ=255)
                                                       gATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCgg                                                                                                <  1:2286663/150‑1 (MQ=255)
                                                        aTCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGGTGTTGCCGATCCTGAAAAAAATTTTTAAGGAAGACAAACCggg                                                                                               >  2:2274324/1‑150 (MQ=255)
                                                         tCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGc                                                                                              >  1:1084089/1‑150 (MQ=255)
                                                          cTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGCGGGTTTTACTTGGGGGTGTTTGCGGTTCTTGAAAAAAATTTTTAAGG‑CGACAAAACggggt                                                                                             >  2:4132342/1‑148 (MQ=255)
                                                            gAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTa                                                                                           <  1:1056943/150‑1 (MQ=255)
                                                            gAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTa                                                                                           <  1:51040/150‑1 (MQ=255)
                                                            gAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGATGTTGCCGATCCTGAAAAAGATTTTTAAGGACGACAAACCGGGCTTa                                                                                           >  2:2539573/1‑150 (MQ=255)
                                                            gAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGGCGACAAACCGGGCTTa                                                                                           >  1:3609119/1‑150 (MQ=255)
                                                             aaaTCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTaa                                                                                          <  1:3829970/150‑1 (MQ=255)
                                                              aaTCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGGTGTTGCCGATCCTGAAAAAGAATTATAAGGACGACAAACCGGGCTTAAg                                                                                         >  2:3901953/1‑150 (MQ=255)
                                                               aTCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTAACTGGGCGGTGTTGTCGATCCTGAAAAAGAATTATAAGGAAGGCAAAACCGGCTTaaac                                                                                        >  2:1692692/1‑148 (MQ=255)
                                                                tCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAgcg                                                                                       >  1:3177298/1‑150 (MQ=255)
                                                                  aGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGcgcg                                                                                     <  1:1870146/150‑1 (MQ=255)
                                                                  aGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTTGGGGATGTTTCCGATCCTGAAAAAGATTTTTTAGGACGAAAAACCAGGGTTaaagccg                                                                                     >  2:1636425/1‑145 (MQ=255)
                                                                  aGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGcgcg                                                                                     >  1:3680860/1‑150 (MQ=255)
                                                                   gTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCgcgg                                                                                    >  1:4035225/1‑150 (MQ=255)
                                                                    tAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCgcggc                                                                                   >  1:3233425/1‑150 (MQ=255)
                                                                      aaaaaGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGGAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGa                                                                                 >  1:2164873/1‑150 (MQ=255)
                                                                       aaaaGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTCCCTTGGCCACGTTGCCGATCCTCAAAAAGACTTTTAAGGACGAGAAAACCGGCTTAAGCGCGGGGAt                                                                                >  2:3597535/1‑150 (MQ=255)
                                                                           gATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGGTGTTGCCGATCCTGGAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGaaa                                                                            >  2:2748317/1‑150 (MQ=255)
                                                                            atatCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAg                                                                           <  1:1443825/150‑1 (MQ=255)
                                                                            atatCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAg                                                                           <  2:2758970/150‑1 (MQ=255)
                                                                              atCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTTCCGGGCGGTGGTGCCGAACCTGAAAAAGATTTTTAAAGACGACAAACCGGGCCTAAGCGCGGGGATGAAAGAt                                                                         >  2:1892748/1‑150 (MQ=255)
                                                                                 aCCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCCGGCTTAAGCGCGGCGGTGAAAGATAAc                                                                      >  1:4084835/1‑150 (MQ=255)
                                                                                  cccGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAAcc                                                                     <  1:3895873/150‑1 (MQ=255)
                                                                                   ccGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCt                                                                    <  1:2500096/150‑1 (MQ=255)
                                                                                     gTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCCGGCTTAAGCGCGGCGATGAAAGATAACCTCg                                                                  >  1:1322351/1‑150 (MQ=255)
                                                                                      tCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGGTGAAAGATAACCTCGa                                                                 >  2:1165345/1‑150 (MQ=255)
                                                                                       cTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGaa                                                                >  1:2263058/1‑150 (MQ=255)
                                                                                       cTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGaa                                                                <  2:2832632/150‑1 (MQ=255)
                                                                                        tGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAAt                                                               <  1:1968816/150‑1 (MQ=255)
                                                                                           gCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGG‑CGGTTTACCGGGGGGGGGTTGGCGATCCCGGAAAAAGTTTATAAAGTCGACGAAGCGGGCTTGAGCGCGGCGAAGAAAGATAACCTCGTAtttt                                                            >  2:1792987/1‑149 (MQ=255)
                                                                                                     gtcgCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATaccca                                                  <  1:2234171/150‑1 (MQ=255)
                                                                                                      tcgCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAAACTCGAATTTTTTAATacccac                                                 >  2:3814444/1‑150 (MQ=255)
                                                                                                          tgctgcAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATacccacccca                                             >  2:3198884/1‑148 (MQ=255)
                                                                                                            ctgcAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAAt                                           <  1:1999026/150‑1 (MQ=255)
                                                                                                            ctgcAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAAt                                           <  1:2484633/150‑1 (MQ=255)
                                                                                                             tgcAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTTCCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAAACTCGAATTTATTAATACCCACCCGAATc                                          >  1:2929899/1‑150 (MQ=255)
                                                                                                             tgcAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATc                                          <  1:3340575/150‑1 (MQ=255)
                                                                                                                  gCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCCATCCTGAAAAAGATTTATAAGGAAGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAAACTCGAATTTATTAATACCCACCCCAATCTGGTc                                     >  2:3337329/1‑150 (MQ=255)
                                                                                                                   cGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCCGGGCGATGTTGTCGAACCTGAAAAAGATTTATAAGGAAGACAAACCGGGGTTAAGCGCGGCGCTGAAAGATAAACACCAAATTATTAATACCCACCCCAAACTGGTCg                                    >  2:4068860/1‑150 (MQ=255)
                                                                                                                     aGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGGTTTACCTTGGGGATGTTGCCGGTCCTGAAAAAGATTTTTAAGGACGACAAACCGGGCTTAAGCGCGGCGGTGAAAAATAAACTCGAAATTATTAATACCCACCCGCAACTGGTCGGa                                  >  2:2067250/1‑150 (MQ=255)
                                                                                                                       cTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGAtt                                <  1:1464570/150‑1 (MQ=255)
                                                                                                                       cTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCCAATCTGGTCGGAtt                                >  1:244475/1‑150 (MQ=255)
                                                                                                                            aCTACGAAAGGATGCAGGCGGGC‑GGGTTTACCCGGGGGATGTTTCCGCTACCTAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGAGGGAAGATAAACTCCCATTTTTTAAACCCCCCCCGAATCTGGTCGGCATCCTGa                           >  2:706393/1‑150 (MQ=255)
                                                                                                                             cTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGGTCCTGAAAAAGATTTATAAGGACGACAAACCGGGGTTAAGCGCGGCGGTGAAAGATAACCTCGAATTTATTAATAACCACCCGAATCTGGTCGGGTTTCTGAt                          >  2:2306739/1‑150 (MQ=255)
                                                                                                                             cTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGAt                          <  1:2898996/150‑1 (MQ=255)
                                                                                                                                cGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCCGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGGTTTCTGATggg                       >  2:368935/1‑150 (MQ=255)
                                                                                                                                   aaGGATGCAGGCGGGC‑GGGTTTTCCTGGGCGATGTTTCCGATCCTGAAAAAGATTTATAAGGACGGCAAACCGGGGTTAAGCGCGGCGATGAAAGATAAACTCCAATTTTTTAATACCCCCCCCCATCTCGTCGGATTTCTGATtggggt                    >  2:2469518/1‑148 (MQ=255)
                                                                                                                                     ggATGCAGGCGGGC‑GGGTTTTCCTGGGCGATGTTTCCGATCCTGAAAAAAATTTTTAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAAACTCGAATTTATTAATACACACCCGAATCTGGTCGGATTCCTGATGGGGgtat                  >  2:2204143/1‑150 (MQ=255)
                                                                                                                                       aTGCAGGCGGGC‑GGGTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCCGGCTTAAGCGCGGCGGTGAAAGATAAACTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGttatta                >  2:1048046/1‑150 (MQ=255)
                                                                                                                                          cAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAAtt             <  1:1597716/150‑1 (MQ=255)
                                                                                                                                          cAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGATATTAAtt             >  1:1715336/1‑150 (MQ=255)
                                                                                                                                           aGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAAttt            >  2:3774739/1‑150 (MQ=255)
                                                                                                                                                    ggTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGgaa    >  2:2584085/1‑150 (MQ=255)
                                                                                                                                                     gTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGgaag   <  1:4124462/150‑1 (MQ=255)
                                                                                                                                                      ttttACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGgaaga  >  1:1648382/1‑150 (MQ=255)
                                                                                                                                                      ttttACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGgaaga  <  1:92921/150‑1 (MQ=255)
                                                                                                                                                      ttttACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGgaaga  <  2:2893933/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
AAGCGCAACCGCAGCCCGTTGCCAGTAAAAATGAAGAAGAGGACTACAGCAATGGGATCTGAAATCAGTAAAAAAGATATCACCCGTCTGGGCTTTCGTTCGTCGCTGCTGCAAGCGAGCTTTAACTACGAAAGGATGCAGGCGGGC‑GGTTTTACCTGGGCGATGTTGCCGATCCTGAAAAAGATTTATAAGGACGACAAACCGGGCTTAAGCGCGGCGATGAAAGATAACCTCGAATTTATTAATACCCACCCGAATCTGGTCGGATTCCTGATGGGGTTATTAATTTCGATGGAAGA  >  AP012306/2758760‑2759058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑