Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306202,4170TG23.3% 657.4 / 38.6 270V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base T (77/130);  new base G (63/0);  total (140/130)
Fisher's exact test for biased strand distribution p-value = 2.02e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 317 total aligned reads displayed.

TTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACCG  >  AP012306/202268‑202566
                                                                                                                                                      |                                                                                                                                                     
 ttCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTa                                                                                                                                                      <  2:2786060/150‑1 (MQ=255)
   cAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGAtt                                                                                                                                                    >  1:3188556/1‑150 (MQ=255)
   cAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACCCTGATTTTGGC‑GGCCGGGGGAtt                                                                                                                                                    >  2:3103170/1‑150 (MQ=255)
      aGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGAGACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGGCGGGGGAttttt                                                                                                                                                 >  2:2470064/1‑148 (MQ=255)
      aGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTtgt                                                                                                                                                 <  1:1628992/150‑1 (MQ=255)
      aGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTtgt                                                                                                                                                 >  1:659490/1‑150 (MQ=255)
      aGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTtgt                                                                                                                                                 >  1:621017/1‑150 (MQ=255)
        cgcCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGa                                                                                                                                               >  1:2265213/1‑150 (MQ=255)
         gcCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGGAGGGGGATTTGTGAc                                                                                                                                              >  1:2571592/1‑150 (MQ=255)
         gcCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGAc                                                                                                                                              <  2:858764/150‑1 (MQ=255)
          ccAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAAAAAATCTGGCAAGAGCCGGGCGGGCCGCCGTGGCCACGTGACACCACCCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGGCAGGGGGATTT‑TGACg                                                                                                                                             >  2:2032090/1‑150 (MQ=255)
            aGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCa                                                                                                                                           <  2:2099753/150‑1 (MQ=255)
            aGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTGACGCa                                                                                                                                           >  1:2384186/1‑150 (MQ=255)
             ggATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTGACGCaa                                                                                                                                          >  1:1260392/1‑150 (MQ=255)
              gATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTTTGGCGCaag                                                                                                                                         >  2:2455940/1‑150 (MQ=255)
                   ctgcATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGgt                                                                                                                                    <  2:3700164/150‑1 (MQ=255)
                     gcATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGgtgt                                                                                                                                  <  2:1762390/150‑1 (MQ=255)
                              cGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAgg                                                                                                                         <  1:2045892/150‑1 (MQ=255)
                              cGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAgg                                                                                                                         >  1:382219/1‑150 (MQ=255)
                                 gCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTga                                                                                                                      <  1:1092678/150‑1 (MQ=255)
                                  cGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTgag                                                                                                                     <  2:3241346/150‑1 (MQ=255)
                                   gTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTgaga                                                                                                                    <  1:856607/150‑1 (MQ=255)
                                   gTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGGTTTTTGACGCAAAAAGGGGGGGGTGGAggggggg                                                                                                                    >  2:1515769/1‑145 (MQ=255)
                                    tAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTGACGCAAGAAGGGGTGGCTGAAGGTgggca                                                                                                                   >  1:3702308/1‑146 (MQ=255)
                                    tAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTGACGCAAGAAGGGGTGGCTGAAGGGGGGaa                                                                                                                   >  1:1450625/1‑150 (MQ=255)
                                      aCGGTGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATg                                                                                                                 <  1:4223481/150‑1 (MQ=255)
                                       cggcggACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATgg                                                                                                                <  1:1540761/150‑1 (MQ=255)
                                        ggcggACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGc                                                                                                               <  1:1003717/150‑1 (MQ=255)
                                          cggACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCgg                                                                                                             >  2:3884736/1‑149 (MQ=255)
                                          cggACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGt                                                                                                             >  2:1167978/1‑150 (MQ=255)
                                           ggACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTGACGCAAGAAGGGGTGGCTGGAGGGGAGAAAGGGGTa                                                                                                            >  2:2592188/1‑150 (MQ=255)
                                             aCGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAg                                                                                                          >  1:1489395/1‑150 (MQ=255)
                                             aCGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTGACGCAAGAAGGGGGGGCTGAAGGGGGGAATGGCGGAAg                                                                                                          >  2:657729/1‑150 (MQ=255)
                                               gTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGtt                                                                                                        >  1:342735/1‑150 (MQ=255)
                                               gTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGtt                                                                                                        <  1:4035582/150‑1 (MQ=255)
                                               gTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGtt                                                                                                        >  1:1769051/1‑150 (MQ=255)
                                               gTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGGAAGtt                                                                                                        >  2:2830641/1‑150 (MQ=255)
                                                 aaGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGAAGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGtttt                                                                                                      >  2:3580035/1‑150 (MQ=255)
                                                   gCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGt                                                                                                    >  1:3733646/1‑150 (MQ=255)
                                                     tAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCt                                                                                                  <  2:1914015/150‑1 (MQ=255)
                                                      aaaGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTg                                                                                                 <  1:3586764/150‑1 (MQ=255)
                                                      aaaGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTg                                                                                                 <  1:1203395/150‑1 (MQ=255)
                                                            aaTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGGTTTTTCTGGCAGaa                                                                                           >  2:3147914/1‑150 (MQ=255)
                                                             aTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCATGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTGACGCAAGAAGGTGTGGATGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGaaa                                                                                          >  2:4310822/1‑150 (MQ=255)
                                                              tCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGaaaa                                                                                         >  1:3237198/1‑150 (MQ=255)
                                                               cTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAAc                                                                                        <  2:3986683/150‑1 (MQ=255)
                                                                tGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGGAAGTTTTGTCTTGCAGAAAACg                                                                                       >  2:3411559/1‑150 (MQ=255)
                                                                 ggCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGc                                                                                      >  1:722651/1‑150 (MQ=255)
                                                                 ggCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTGACGCCAGAAGGTGGGGCTGGAGGGGAGAATGGCGTAAGTTTTTTCTGGGAGAAAAAGc                                                                                      >  2:4368458/1‑150 (MQ=255)
                                                                  gCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCt                                                                                     <  1:2863003/150‑1 (MQ=255)
                                                                    aaGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCCTa                                                                                   >  1:945434/1‑150 (MQ=255)
                                                                     agagCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAg                                                                                  <  1:2149309/150‑1 (MQ=255)
                                                                      gagCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGt                                                                                 >  2:1660568/1‑150 (MQ=255)
                                                                       agCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTGACGCCAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTTGCCGAAAACGGCTAGtt                                                                                >  2:1021580/1‑150 (MQ=255)
                                                                         cTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTGACGCAAGAAGGGGTGGCTGAAGGAGAGAATGGCGTAAGTTTTGTCTGGGAGAAAACGCTTAGTTaa                                                                              >  2:4176032/1‑150 (MQ=255)
                                                                          tGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTGACGCAAGAAGGGGTGGCTGAAGGTGAGAATGGGGTAAGGTTTGTCTGGCAGAAAACGCTTAGTTAAg                                                                             >  2:3906475/1‑150 (MQ=255)
                                                                            ggCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTg                                                                           <  1:1972899/150‑1 (MQ=255)
                                                                             gCGTGCCGCCGTGGCTACGTGACGCCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGa                                                                          <  2:2962572/150‑1 (MQ=255)
                                                                              cGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGaa                                                                         <  2:1333443/150‑1 (MQ=255)
                                                                                 gccgccGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTTGCGCAAGAAGGTGTTGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGGAGAAAACGCTTAGTTAAGTGAAAGc                                                                      >  2:2257158/1‑150 (MQ=255)
                                                                                   cgccgTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCg                                                                    <  2:1471972/150‑1 (MQ=255)
                                                                                        tGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTGACGCAAGAAGGGGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGGTaa                                                               >  2:2206733/1‑150 (MQ=255)
                                                                                         ggCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAg                                                              >  1:3996203/1‑150 (MQ=255)
                                                                                          gCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGa                                                             <  1:1304389/150‑1 (MQ=255)
                                                                                           cTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GACAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGAc                                                            >  1:1715077/1‑150 (MQ=255)
                                                                                             aCGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGc                                                          >  1:2691019/1‑150 (MQ=255)
                                                                                             aCGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGGAAACGCTTAGTTAAGTGAAAACCGGGTAAGACGc                                                          >  2:3808632/1‑150 (MQ=255)
                                                                                              cGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAAAGCTTAGTTAAGTGAAAACCGGATAAGACGCa                                                         >  2:4112669/1‑150 (MQ=255)
                                                                                                tGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATc                                                       <  1:1231354/150‑1 (MQ=255)
                                                                                                tGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATc                                                       >  1:3108182/1‑150 (MQ=255)
                                                                                                 gACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCa                                                      >  2:544735/1‑150 (MQ=255)
                                                                                                   caccacGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAACCGGATAAGACGCCTCaaa                                                    >  2:1946808/1‑150 (MQ=255)
                                                                                                     ccacGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACg                                                  >  2:2912880/1‑150 (MQ=255)
                                                                                                      cacGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGt                                                 <  2:1145546/150‑1 (MQ=255)
                                                                                                       acGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTc                                                <  1:4273495/150‑1 (MQ=255)
                                                                                                       acGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTc                                                >  1:1352999/1‑150 (MQ=255)
                                                                                                         gCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGc                                              <  2:1195319/150‑1 (MQ=255)
                                                                                                          ccACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCa                                             <  2:230850/150‑1 (MQ=255)
                                                                                                           cACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCAt                                            >  1:1078374/1‑150 (MQ=255)
                                                                                                           cACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGGTTTGTCTGGCAGAAAACGCTTTGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCAt                                            >  2:1891105/1‑150 (MQ=255)
                                                                                                             ctgctgTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATcc                                          >  1:573664/1‑150 (MQ=255)
                                                                                                                  gTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGa                                     >  1:298427/1‑150 (MQ=255)
                                                                                                                    tttATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGaaa                                   >  1:1650307/1‑150 (MQ=255)
                                                                                                                               aCGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGGGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGa                        >  2:1276189/1‑150 (MQ=255)
                                                                                                                                cGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGAt                       <  2:4328769/150‑1 (MQ=255)
                                                                                                                                  cTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTc                     >  1:498236/1‑150 (MQ=255)
                                                                                                                                   tGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCg                    <  1:2216405/150‑1 (MQ=255)
                                                                                                                                      ttGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCtt                 <  2:1083605/150‑1 (MQ=255)
                                                                                                                                       tGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTa                <  2:725670/150‑1 (MQ=255)
                                                                                                                                       tGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTa                <  2:1811191/150‑1 (MQ=255)
                                                                                                                                        gcggc‑gGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTac               <  2:2728346/150‑1 (MQ=255)
                                                                                                                                          ggc‑ggcAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTacca             >  1:3633038/1‑150 (MQ=255)
                                                                                                                                           gc‑ggcAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTaccac            <  1:3510570/150‑1 (MQ=255)
                                                                                                                                            c‑ggcAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTaccacc           >  1:4172747/1‑150 (MQ=255)
                                                                                                                                              ggcAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTaccacca          >  2:3027791/1‑150 (MQ=255)
                                                                                                                                                cAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTaccaccacc        >  1:4406782/1‑150 (MQ=255)
                                                                                                                                                 aGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCg       >  2:427821/1‑150 (MQ=255)
                                                                                                                                                 aGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCg       >  1:1423972/1‑150 (MQ=255)
                                                                                                                                                    ggTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTAc    >  1:4081581/1‑150 (MQ=255)
                                                                                                                                                     gTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTAcc   <  2:2368240/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TTTCAAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGC‑GGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACCG  >  AP012306/202268‑202566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑