Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306341,3770TG27.4% 256.2 / 19.8 146G60G (GGT→GGG) ribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
Reads supporting (aligned to +/- strand):  ref base T (21/85);  new base G (40/0);  total (61/85)
Fisher's exact test for biased strand distribution p-value = 9.69e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 221 total aligned reads displayed.

CTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGG  >  AP012306/341228‑341524
                                                                                                                                                      |                                                                                                                                                   
  aaaGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGa                                                                                                                                                   <  2:4069925/150‑1 (MQ=255)
  aaaGCTGGCGCAACGAGGACGTTTTACCACACATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGa                                                                                                                                                   >  2:321921/1‑150 (MQ=255)
       tGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAg                                                                                                                                              <  1:2570638/150‑1 (MQ=255)
        ggCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGc                                                                                                                                             <  1:3464797/150‑1 (MQ=255)
        ggCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGc                                                                                                                                             <  1:2030695/150‑1 (MQ=255)
           gcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCaa                                                                                                                                          <  1:3293299/150‑1 (MQ=255)
            cAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAAACCaa                                                                                                                                          >  2:4083655/1‑150 (MQ=255)
             aaCGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAACCCaaa                                                                                                                                         >  1:617701/1‑150 (MQ=255)
             aaCGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAg                                                                                                                                        <  1:886909/150‑1 (MQ=255)
             aaCGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCaaaa                                                                                                                                        >  1:3712173/1‑149 (MQ=255)
               cGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGt                                                                                                                                      <  2:821498/150‑1 (MQ=255)
               cGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGt                                                                                                                                      <  2:358081/150‑1 (MQ=255)
               cGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGt                                                                                                                                      <  1:2731988/150‑1 (MQ=255)
               cGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGt                                                                                                                                      <  1:2366588/150‑1 (MQ=255)
               cGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAggc                                                                                                                                      >  1:1561337/1‑149 (MQ=255)
                gAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGGAAAAAACAAAAggg                                                                                                                                      >  2:3760329/1‑149 (MQ=255)
                gAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTg                                                                                                                                     <  1:4153864/150‑1 (MQ=255)
                 aGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGc                                                                                                                                    <  2:2747240/150‑1 (MQ=255)
                 aGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGc                                                                                                                                    <  1:4037220/150‑1 (MQ=255)
                    aCGTTTTACCACGCATCCCAACCCGAAGGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGAc                                                                                                                                 <  1:1733596/150‑1 (MQ=255)
                     cGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCCAAAGGGCGAcc                                                                                                                                >  1:1847317/1‑150 (MQ=255)
                         ttACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCt                                                                                                                            <  2:2597602/150‑1 (MQ=255)
                         ttACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCt                                                                                                                            <  1:4401627/150‑1 (MQ=255)
                         ttACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCt                                                                                                                            <  2:1563998/150‑1 (MQ=255)
                         ttACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAAGCAAAAGTGCGACCGCCt                                                                                                                            >  1:500695/1‑150 (MQ=255)
                         ttACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGAGCGACCGccc                                                                                                                            >  1:4232934/1‑149 (MQ=255)
                          tACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGTGCGAACGccca                                                                                                                           >  1:3536292/1‑148 (MQ=255)
                          tACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGCGACCGccca                                                                                                                           >  1:2257530/1‑148 (MQ=255)
                              aCGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTc                                                                                                                       <  1:738335/150‑1 (MQ=255)
                                 cATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTCCCCGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTcaca                                                                                                                    <  2:2847049/150‑1 (MQ=255)
                                 cATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGTGCGACCGCCCCTGTcaca                                                                                                                    >  1:1934893/1‑150 (MQ=255)
                                 cATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGGGCGGCCCCCCATTTcaca                                                                                                                    >  2:1514280/1‑150 (MQ=255)
                                    cccACCCCGAATGTCGGGTGCGTCATTGTAAAAGATGGCGAAATGGTCGGTGAAGGTTACCACCAACGTGGGGGTGAACCACATCCCGAAGTCCCCGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTc                                                                                                                 <  2:2674000/150‑1 (MQ=255)
                                      cAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGa                                                                                                               <  1:3275497/150‑1 (MQ=255)
                                       aaCCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGGCAAAAGGGCGACCCCCCATGTCACACTccga                                                                                                              >  1:4250333/1‑147 (MQ=255)
                                       aaCCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAAACAAAAGGGGGGCCCCCCATGTCccaccccaa                                                                                                              >  2:162689/1‑145 (MQ=255)
                                        ccccGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAATTTGTCGGTGAAGTTTCCCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCTTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAAc                                                                                                             <  2:3670713/149‑1 (MQ=255)
                                        ccccGAATGTCGGGTGCGTCATTGTCAAAGAGGGCGAAATTGGGGGGGAAGGTTACCACCAACGTGGGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGG‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAAc                                                                                                             <  2:1205237/149‑1 (MQ=255)
                                        aCCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAACCAAAAGTGCGACCGCCCATGTCACACTccgac                                                                                                             >  1:955776/1‑146 (MQ=255)
                                         ccggAATGTGGGGTGCGTAATTGTCAAAGAGGGCGAAATTGTGGGTGAAGTTTCCCCCCAAGGTGGGGGTGAACCACATGCGGAAGTCCACGGGTGGCTTTGGGC‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAAcc                                                                                                            <  2:3050957/147‑1 (MQ=255)
                                         ccccAATGTCGGGTGCTTCATTGTCAAACATGCCAAATTTGTCGGTGAAGTTTCCCCCCACCGTCCGCGTCACCCCCATCCCCACCTCCCCCCTTTCCTTCCGCC‑GGTTCAAAACCCCAACGGTCCCCCCGCCTATGTCACACTCGAAcc                                                                                                            <  2:385843/150‑1 (MQ=255)
                                            gAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGGGGGACCGCCCATGTCACACTCGAAccccg                                                                                                         >  2:4229150/1‑148 (MQ=255)
                                             aaTGTCGGGTGCGTCATTGTAAAAGATGGCAAAATTGTCGGTGAAGGTTACCACCAACGGGGGGGTGAACCACATGCCGAAGTACACGCGTTGCGTAGGGC‑GGGTAAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGt                                                                                                        <  2:4353243/150‑1 (MQ=255)
                                                gTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGc                                                                                                     <  1:1330291/150‑1 (MQ=255)
                                                   gggTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAGCCAAAGGGGCGGCCGCCCCTGTCCCCCCCGAACCCTGTGGCcat                                                                                                  >  2:2627849/1‑150 (MQ=255)
                                                    ggTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCGGAAGTACACGCGTTGCGTATGGC‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCcatc                                                                                                 <  1:3827389/150‑1 (MQ=255)
                                                    ggTGCGTCATTGAAAAAGTGGGCAAAGTTGTTGGGGAAGGTTCCCCCGATGGGGGGGGTGAACCACGTGCTGAAGTACAGGCGTTGGGTGGGGG‑GGGTAAAAAAGCAAAGGGTGCAACCGCCTATGTCACACTCGAACCCTGTAGCcatc                                                                                                 <  2:1056686/150‑1 (MQ=255)
                                                      ggcttCATTGTAAAAGAGGCCGAAATTGCCGGGGAAGTTTACCACAAAGGGGGGGGAAAACCACAGGCCGAAGTACACGCGTTGCGTAGGGC‑GGGTGAAAAAGCAAAAGGGGCAACCGCCTATGTCACACTCGAACCCTGTAGCcatcat                                                                                               <  2:562045/146‑1 (MQ=255)
                                                        cGTCATTGTCAAAGATGGCGAAATTGTGGGTGAAGGTTCCCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATgg                                                                                             <  1:1493067/150‑1 (MQ=255)
                                                        cGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAACCAAAAGGGCGACCGCCCATGTCACACTCGAACCCTGTAGCCATCATgg                                                                                             >  1:1303805/1‑150 (MQ=255)
                                                           cATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAAACAAAAGGGCGACCGCCCATGTCACACTCCAACCCTGTAGCCATCCTGGGCg                                                                                          >  2:4289710/1‑150 (MQ=255)
                                                             ttGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGTGCGACCCCCCATTTCACACTCCAACCCTGTAGCCATCATGGTCGTa                                                                                        >  2:1770523/1‑150 (MQ=255)
                                                             ttGTAAAAGAGGGCGAAATTGTGGGTGAAGGTTACCACCACCGTGCGGGTAACCAACAGGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCAAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTa                                                                                        <  2:1487036/150‑1 (MQ=255)
                                                              tGTCAAAGATGGCGAATTTGTCGGTGAAGGTTCCCACCAACGGGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAGGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTAc                                                                                       <  2:3515313/150‑1 (MQ=255)
                                                                tCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAAAAAAAAGGGCGACCCCCTATGTGTCACTCGGAGCCTGTCGGCATCATGGTCGTTCGc                                                                                     >  2:2306432/1‑150 (MQ=255)
                                                                  aaaGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAAGCAAAAGAGGGACCCCCCATGTCCCACCCCAAACCCGTAGGCATCATGGTCGGTCGccc                                                                                   >  2:2112659/1‑149 (MQ=255)
                                                                  aaaGATGGCAAAATTGTCGGTGAAGGTTCCCCCCACCGTGCGGGTAAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTAAAAAAGCAAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCa                                                                                   <  2:2350360/150‑1 (MQ=255)
                                                                   aaGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGGAAAAAACAAAAGGGCGACCGCCTATGTCACACTCCAAACCCGTAGCCCTCCTGGTCGGACGCCAc                                                                                  >  2:2005260/1‑150 (MQ=255)
                                                                    aGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGGGGGACCCCCCATGTCACACCCCCACCCCCTAACCACCAAGGGCGGACGCcccc                                                                                 >  2:2220501/1‑150 (MQ=255)
                                                                            aaaTTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCtgtg                                                                         <  1:288343/150‑1 (MQ=255)
                                                                             aaTTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGa                                                                        >  1:1363163/1‑150 (MQ=255)
                                                                               ttGTGGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACg                                                                      <  1:56066/150‑1 (MQ=255)
                                                                                   cGGTGAAGGTTACCACCAACGTGGGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACt                                                                  <  1:1196245/150‑1 (MQ=255)
                                                                                   cGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACt                                                                  <  1:1878786/150‑1 (MQ=255)
                                                                                        aaGGTTACCACCACCGGGCGGGTGAACCACTTCACCAATTCCACGCGTTGCGTGTGGC‑GGGTGAAAAACCGAAGGGTCCGCCCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATcg                                                             <  2:1462974/150‑1 (MQ=255)
                                                                                         aGGTTCCCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTGGCGTAGGGG‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATcgc                                                            <  2:1865244/150‑1 (MQ=255)
                                                                                              ccccccaACGGGCGGGTAACCCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTg                                                       <  2:2985278/146‑1 (MQ=255)
                                                                                                caccaGCGTGGGGGTAAACCACATGCCGAAGTACCCGCGTTGCGTAGGGG‑GGGTGAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGc                                                     <  2:674129/150‑1 (MQ=255)
                                                                                                   cacggggCGGGAAAACCAATTCCCGAAGTCCCCGCGTGGGTTAGGGG‑GGGTGAAAAAGCCAACGGGGCAGCCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTa                                                  <  2:790786/144‑1 (MQ=255)
                                                                                                               gAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAACCAAAAGGGGGACCGCCCATGTCACACTCGAAACCTGTAGCCATCCTGGTCGGACGCCACCCCGCTGTGACGCACTCACCGCCGCCCGGGGAGCGCGCgggggt                                      >  2:929020/1‑148 (MQ=255)
                                                                                                                  ccACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGcc                                   <  1:2862195/150‑1 (MQ=255)
                                                                                                                  ccACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTAAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGcc                                   <  2:3945611/150‑1 (MQ=255)
                                                                                                                    acaTGCCGAAGTACCGGCGTTGGGTAGGGG‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTc                                 <  2:1386548/150‑1 (MQ=255)
                                                                                                                    acaTGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTc                                 <  1:1820908/150‑1 (MQ=255)
                                                                                                                     caTGCCGAAGTACGCGCGTTGCGTAGGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCg                                <  2:3417735/150‑1 (MQ=255)
                                                                                                                      aTGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGCGGCCCCCCCTTTCACACTCCAACCCCGTAGCCCTCATGGTCGTACGCCCCCGTGCTGTGGCGCCCTCATCGCCCCCGGCGGAGCGCGCGGGGGTggcccca                               >  2:18268/1‑144 (MQ=255)
                                                                                                                       tGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAGGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGAt                              <  1:3206793/150‑1 (MQ=255)
                                                                                                                       tGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGAt                              <  1:1399611/150‑1 (MQ=255)
                                                                                                                       tGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGAt                              <  1:2461973/150‑1 (MQ=255)
                                                                                                                         ccGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGc                            <  1:4277105/150‑1 (MQ=255)
                                                                                                                         ccGAAGTACACGCGTTGCGTATGGC‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGc                            <  1:3372924/150‑1 (MQ=255)
                                                                                                                         ccGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGc                            >  1:577359/1‑150 (MQ=255)
                                                                                                                            aaGTACCCGCGTTGCGTAGGGG‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAg                         <  1:3069097/150‑1 (MQ=255)
                                                                                                                             aGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGa                        <  1:3640377/150‑1 (MQ=255)
                                                                                                                              gTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGAt                       <  1:2316207/150‑1 (MQ=255)
                                                                                                                              gTACACGCGTGGGGTATGGG‑GGGTAAAAAACCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGAt                       <  2:2057020/150‑1 (MQ=255)
                                                                                                                                  acGCGTTGCGTATGGC‑GGGGGAAAAAACCAAAAGGGCGACCGCCCATGTCACACTCGAAACCCGTAGCCCTCATGGGCGTACGCCCCCGTGCTGTGACGCCCTCATCGCCCCTGGCGTAGCGCGCGTGGGTGCCCCGATGCAAGATCCTa                   >  2:3702915/1‑150 (MQ=255)
                                                                                                                                  acGCGTTGCGTATGGC‑AGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTa                   >  2:3113211/1‑150 (MQ=255)
                                                                                                                                     cgTTGGGTAGGGG‑GGGAGAAAAAGCAAAAGGGGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAAcc                <  2:4254778/150‑1 (MQ=255)
                                                                                                                                      gtggCGTAGGGC‑GGGAAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAAccc               <  1:4011159/147‑1 (MQ=255)
                                                                                                                                      gTTGCGTATGGG‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAAccc               <  2:2684444/150‑1 (MQ=255)
                                                                                                                                      gTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAAccc               <  1:2783761/150‑1 (MQ=255)
                                                                                                                                          cGTAGGGC‑GGGTAAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAg           <  1:1617766/150‑1 (MQ=255)
                                                                                                                                           taaTGGG‑GGGGAAAAAAGCAAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAgg          <  1:4374403/148‑1 (MQ=255)
                                                                                                                                           gTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAACGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAgg          >  2:2338495/1‑150 (MQ=255)
                                                                                                                                              tGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCg       >  1:4085125/1‑150 (MQ=255)
                                                                                                                                              tGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGGCg       >  2:3294855/1‑150 (MQ=255)
                                                                                                                                                 c‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTg    >  1:251184/1‑150 (MQ=255)
                                                                                                                                                   gggAGAAAAAGCAAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTgg   <  1:2974823/150‑1 (MQ=255)
                                                                                                                                                    ggTAAAAACGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCGCCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTggg  <  2:1661901/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
CTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGG  >  AP012306/341228‑341524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑