Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123062,212,7530AC27.7% 374.5 / 53.8 159Y241D (TAT→GAT) hcaTpredicted 3‑phenylpropionic transporter
Reads supporting (aligned to +/- strand):  ref base A (104/11);  new base C (0/44);  total (104/55)
Fisher's exact test for biased strand distribution p-value = 3.16e-29
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 213 total aligned reads displayed.

CAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAACCGG  >  AP012306/2212604‑2212899
                                                                                                                                                      |                                                                                                                                                  
  aCGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATAcc                                                                                                                                                  >  1:582245/1‑150 (MQ=255)
  aCGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATAcc                                                                                                                                                  >  1:1375939/1‑150 (MQ=255)
   cGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATAccc                                                                                                                                                 <  1:1470047/150‑1 (MQ=255)
    gCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATAcccc                                                                                                                                                <  2:3638378/150‑1 (MQ=255)
      cGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGGAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCAc                                                                                                                                              >  1:2074749/1‑150 (MQ=255)
      cGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATAcccccc                                                                                                                                              >  2:3152091/1‑148 (MQ=255)
       gTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCAcc                                                                                                                                             <  1:855172/150‑1 (MQ=255)
        tAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATCCCCCAccg                                                                                                                                            <  2:3748567/150‑1 (MQ=255)
           ttGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATCCCCCAccgccg                                                                                                                                         <  1:165014/150‑1 (MQ=255)
               tCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAACGCAAAGATAATGAATT‑CCGCCACCACGCCCAGCGACCACAAATACCCCCCCgccgaggc                                                                                                                                     >  2:4075265/1‑150 (MQ=255)
                   aTAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCgcccaggccgag                                                                                                                                 >  2:2670385/1‑150 (MQ=255)
                   aTAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACCCCCAGCGACCACAAATACCCCACCgccgaggccgag                                                                                                                                 >  2:3868901/1‑150 (MQ=255)
                    tAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGt                                                                                                                                >  1:926970/1‑150 (MQ=255)
                    tAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTC‑CCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGt                                                                                                                                <  2:167314/150‑1 (MQ=255)
                     aaTGCCCCAGCGCACTAAGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCCCAAATACCCCACCgccgaggccgagga                                                                                                                               >  2:1011543/1‑148 (MQ=255)
                        cccccAGCGCACTCCGCCGAAAACCGCCGAGATAAACAGCATACCGCGTCCACTACACCGGGGAAAAATTTTATTCCTCAGCGAAAAAAAAATGACTC‑CCCCCCCCCCCCCCAGCGACCAAAAATCCCCCACCGCCGAGGCCGAGTAGcc                                                                                                                            <  2:2079598/149‑1 (MQ=255)
                            aaGCGCACTACGCCGAAACTCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTC‑CCGCCACCCCGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGct                                                                                                                        <  2:2418039/149‑1 (MQ=255)
                             aGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGctg                                                                                                                       >  1:3290531/1‑150 (MQ=255)
                                   cTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCtgcctgcc                                                                                                                 >  1:3965204/1‑150 (MQ=255)
                                     aCGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAg                                                                                                               >  2:3088415/1‑150 (MQ=255)
                                      cgccgcAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGt                                                                                                              >  1:3564533/1‑150 (MQ=255)
                                      cgccgaAAACCGCCGAGATCAACAGCATATCGCGTGCCCTACAACGGCGAAAAATTTTATTACTCAGCGAAAAGATAATGCCTC‑CCGCCACCCCGCCCAGCGACCAAAAATCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGt                                                                                                              <  2:2631962/150‑1 (MQ=255)
                                       gccgcAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAAGGGCGAAAAAGTTTATTACTCAGCGCAAAGATAATGACTC‑CCGCCACCCCGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTa                                                                                                             <  1:4045907/150‑1 (MQ=255)
                                       gccgcAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCCGCCAGTa                                                                                                             >  2:1322437/1‑150 (MQ=255)
                                           cAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATg                                                                                                         >  1:2660151/1‑150 (MQ=255)
                                           cAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGGTTAGTACTCAGGGCAAAGATAATGAATT‑CCGCCACCACGACCAGGGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCGGCCAGGAAATg                                                                                                         >  2:1849351/1‑150 (MQ=255)
                                           aaaaCCGCCGAAACAAACAGCATATCGCGGGCACTACAACGGCGAAAATTTTTATTCCACACGGAAAAGA‑AAAGCCTTCCCGCCCCCCCCCCAAGCACCCAAAAATCCCCCCCCGCCGAGGCGGAGTAGCCAGCTGCCTGCCAGTAAATg                                                                                                         <  2:3576070/149‑1 (MQ=255)
                                              aTCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGgcg                                                                                                      >  2:2800677/1‑150 (MQ=255)
                                                cGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCt                                                                                                    <  1:1877469/150‑1 (MQ=255)
                                                 gCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTa                                                                                                   >  1:2201494/1‑150 (MQ=255)
                                                 gCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTa                                                                                                   >  2:2961382/1‑150 (MQ=255)
                                                        tCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGt                                                                                            >  1:2288732/1‑150 (MQ=255)
                                                          aaCAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTaa                                                                                          >  2:2080606/1‑150 (MQ=255)
                                                           aCAGCATATCCCGTGCACTACAACGGCGGAAAAGTTTTTTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCCCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAAt                                                                                         >  2:3659296/1‑150 (MQ=255)
                                                            cAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCACTAAAT‑GCGCTAAAACCGTAATAg                                                                                       >  1:428779/1‑150 (MQ=255)
                                                               cATATCGCGTCCACTACACCGGCGAAAAATTTTATTACTCAGCGAAAAGATAATGCCTC‑CCGCCACCCCGCCCAGCGCCCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGc                                                                                     <  2:4280251/150‑1 (MQ=255)
                                                                atatCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCT‑AAACCGTAATAAGCCg                                                                                   >  2:1344004/1‑150 (MQ=255)
                                                                atatCGCGTGCACTACAACGGCGAAAAATTTTATTCCTCAGCGCAAAGATAATGCCTC‑CCGCCCCCCCGCCCAGCGCCCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGcc                                                                                    <  2:729470/150‑1 (MQ=255)
                                                                atacCGCGGCCACTAAAGCGGCGGAAAAGTTTTTTACAAAGCGCAAAGATAATGCCTG‑CCGCCCCCACGCCGGGCGACCAAAATCCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGcc                                                                                    <  2:1569698/150‑1 (MQ=255)
                                                                    cgcgTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGCCTT‑CCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCAt                                                                                <  1:1771906/150‑1 (MQ=255)
                                                                    cgcgTGCACTACAACGGCGAAAAAGTTTATTACTCAGCGCAAAGAAAATGCCTC‑CCCCCACCCCGCCCAGCGACCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCAt                                                                                <  2:274352/150‑1 (MQ=255)
                                                                    cgcgTGCACTACAACGGCGAAAAAGTTTATTACTCAGCGAAAAGAAAATGCCTC‑CCGCCCCCCCGCCCAGCGCCCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCAt                                                                                <  2:3382449/150‑1 (MQ=255)
                                                                      ggTGCCCTACAACGGGGAAAAATTTTATTACCCGGCGAAAAGATAAGGACTC‑CCGCCACCACGCCCAGCGCCCACAAACCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCAtgt                                                                              <  2:2170876/149‑1 (MQ=255)
                                                                      cgTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCAtgt                                                                              >  1:2871343/1‑150 (MQ=255)
                                                                      cgTGCACAACAAGGGCGAAAAAGTTTATTCCCCAGCGAAAAAATAATGACTC‑CCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCAtgt                                                                              <  2:1638210/150‑1 (MQ=255)
                                                                       gttcACTACAACGGCGGAAAAGGTTATTACCCAGCGCAAAGATAATGACTT‑TCCCCACCACGCCCAGCCACCACAAAAACCCCACCCCCCACGCCTAGTAGCCAGCTGCCTGCCAGTAAATTGCGCTCAAACCATAATAAGCCGCAttgg                                                                             >  2:892277/4‑147 (MQ=255)
                                                                            caacacCGGCGAAAAAGTTTATTCCACAGCGCAAAGATAATGCCTC‑CCCCCACCACGCCCAGCCCCCAAAAATCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGccccc                                                                        <  2:4298899/148‑1 (MQ=255)
                                                                            cTACAGCGGCGAAAAATTTTTTTCCCCAGCGAAAAAAAAATGCCTT‑CCCCCCCCCCGCCCGGCGCCCAAAAATCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGccccc                                                                        <  2:4093570/150‑1 (MQ=255)
                                                                            cTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGccccc                                                                        >  1:1002814/1‑150 (MQ=255)
                                                                               cAACGGCGGAAAAGTTTATTACTCAGCGAAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGc                                                                     <  1:340532/150‑1 (MQ=255)
                                                                                aaCGGCGAAAAAGTTTATTACCCAGCGCAAAGATAATGCCTC‑CCGCCCCCCCGCCAAGCGACCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCa                                                                    <  2:2552470/150‑1 (MQ=255)
                                                                                 aCGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCaa                                                                   >  1:184414/1‑150 (MQ=255)
                                                                                  cggggaaaaaTTTTATTACCAGGCGCAAAAATATAATCTG‑CCGCCCCCCCGCCCAGGAACCAAAAAACCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAAt                                                                  <  2:846736/144‑1 (MQ=255)
                                                                                   ggcggAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATa                                                                 >  2:1893335/1‑150 (MQ=255)
                                                                                     cggAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaa                                                               >  2:290915/1‑150 (MQ=255)
                                                                                     cgaaaaaTTTTATTCCTCAGCGAAAAGATAATGTCTC‑CCGCCACCACCCCCAGCGACCAAAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaa                                                               <  2:352266/147‑1 (MQ=255)
                                                                                      ggAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaac                                                              >  2:3718914/1‑150 (MQ=255)
                                                                                      gaaaaaGTTTATTACCCAGCCAAAAAATAAGGGCTC‑CCGCCCCCACGCCCGGCGCCCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaac                                                              <  2:3473841/148‑1 (MQ=255)
                                                                                        aaaaGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaacaa                                                            >  1:3765357/1‑150 (MQ=255)
                                                                                         aaaTTTTATCCCCCAGCGAAAAAAAAAGGCCTC‑CCGCCACCACCCCCAGCGCCCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaacaaa                                                           <  2:3351842/150‑1 (MQ=255)
                                                                                          aggTTTATTACTCAGCGAAAAGAAAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaacaaac                                                          <  1:468879/148‑1 (MQ=255)
                                                                                          aaGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATaaacaaac                                                          >  1:2721973/1‑150 (MQ=255)
                                                                                            gTTTATTCCCCAGCGCAAAAATAATGCCTC‑CCGCCACCACGCCCAGCGCCCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGc                                                        <  2:1842147/150‑1 (MQ=255)
                                                                                             tttATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCa                                                       >  2:1102793/1‑150 (MQ=255)
                                                                                             tttATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCa                                                       >  1:2487616/1‑150 (MQ=255)
                                                                                              ttattaCTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAg                                                      >  2:3608124/1‑150 (MQ=255)
                                                                                              ttattaCTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAg                                                      >  1:2162728/1‑150 (MQ=255)
                                                                                               tattaCTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAgg                                                     <  1:3466692/150‑1 (MQ=255)
                                                                                                tttaCCCAGCGAAAAGAAAATGCCTC‑CCGCCACCACGCCCAGCCCCCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGc                                                    <  2:311992/149‑1 (MQ=255)
                                                                                                 ttaCTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGcc                                                   >  2:1877466/1‑150 (MQ=255)
                                                                                                   aCTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAg                                                 >  2:333998/1‑150 (MQ=255)
                                                                                                    cTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATTTGCCCCCTGCAATAAACAAACGCAGGCCAGa                                                >  1:4028132/1‑150 (MQ=255)
                                                                                                    cTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGa                                                >  1:4303033/1‑150 (MQ=255)
                                                                                                     gcaccgcAACGAGAGTGACTT‑CCGCCACCACGCCCACCGCCCACAACTACCCAACCGCCGTGGCCGAGTAGCCAGCTGCCTGCCAGGAAAGGACGCTAAAACCGTAATAGCACACATGTTCCCCCCGCACTAAACAAACGCAGGCCAgca                                               >  2:518841/5‑148 (MQ=255)
                                                                                                      cAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGaaa                                              >  1:2512954/1‑150 (MQ=255)
                                                                                                        gcgaAAAGATAATGACTC‑CCGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAgc                                            <  1:1347290/150‑1 (MQ=255)
                                                                                                             aaGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAg                                       >  1:3188988/1‑150 (MQ=255)
                                                                                                                 tAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCt                                   >  2:3883859/1‑150 (MQ=255)
                                                                                                                    tGACTT‑CCGCCCCCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGc                                <  1:3847894/150‑1 (MQ=255)
                                                                                                                      gcctcccGCCACCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCg                               <  1:2449636/145‑1 (MQ=255)
                                                                                                                           ccGCCACCACCCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCa                          <  2:486032/150‑1 (MQ=255)
                                                                                                                            cccccccacCCCCAGCGCCCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAg                         <  2:418676/145‑1 (MQ=255)
                                                                                                                            cGCCCCCACGCCCAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAg                         <  1:3838413/150‑1 (MQ=255)
                                                                                                                             gCCCCCACGCCGAGCGCCCAAAAACCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAgc                        <  1:2095155/150‑1 (MQ=255)
                                                                                                                             gCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAgc                        >  1:455072/1‑150 (MQ=255)
                                                                                                                               caccacGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAgcgc                      >  1:106413/1‑150 (MQ=255)
                                                                                                                               caccacGCCCAGCGACCAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAgcgc                      <  1:112656/150‑1 (MQ=255)
                                                                                                                                accacGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGcc                     >  2:4183307/1‑150 (MQ=255)
                                                                                                                                accacGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGcc                     >  2:3240171/1‑150 (MQ=255)
                                                                                                                                accacGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGcc                     >  1:672829/1‑150 (MQ=255)
                                                                                                                                  cccGCCCAGCGCCCAAAAACCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCaa                   <  2:673143/148‑1 (MQ=255)
                                                                                                                                     gCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCa                >  1:3258330/1‑150 (MQ=255)
                                                                                                                                       ccAGCGACCACAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACg              <  1:204685/150‑1 (MQ=255)
                                                                                                                                        cAGCGCCCACAAACCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGc             <  2:1751942/150‑1 (MQ=255)
                                                                                                                                        cAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGc             >  2:1071446/1‑150 (MQ=255)
                                                                                                                                            gACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGAc         >  2:586111/1‑150 (MQ=255)
                                                                                                                                             aCCAAAAACACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGAcc        <  1:4196217/150‑1 (MQ=255)
                                                                                                                                              ccAAAAATCCCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCa       <  1:4375968/150‑1 (MQ=255)
                                                                                                                                               caaaaaTCCCCCCCCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCaa      <  1:1388085/147‑1 (MQ=255)
                                                                                                                                                 caAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAAcc    >  1:2396644/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
CAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT‑CCGCCACCACGCCCAGCGACCACAAATACCCCACCGCCGAGGCCGAGTAGCCAGCTGCCTGCCAGTAAATGGCGCTAAAACCGTAATAGGCCGCATGTGCCCCCTGCAATAAACAAACGCAGGCCAGAAAGCGCCAGTTCTGGCGAACCAGCGCCAACCACGCAGACCAACCGG  >  AP012306/2212604‑2212899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑