Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,020,6700TG33.3% 383.0 / 11.5 219K195N (AAA→AAC) glgCglucose‑1‑phosphate adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (102/44);  new base G (73/0);  total (175/44)
Fisher's exact test for biased strand distribution p-value = 1.54e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 299 total aligned reads displayed.

ATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTCTTGCTT  >  AP012306/3020521‑3020819
                                                                                                                                                      |                                                                                                                                                     
aTCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGt                                                                                                                                                       <  2:2647065/150‑1 (MQ=255)
 tCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGtt                                                                                                                                                      <  2:991871/150‑1 (MQ=255)
   aaaTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGgttt                                                                                                                                                    >  1:1720973/1‑150 (MQ=255)
    aaTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGgtttt                                                                                                                                                   >  1:1392920/1‑150 (MQ=255)
    aaTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGTC‑GGatttt                                                                                                                                                   >  2:3785101/1‑150 (MQ=255)
     aTCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATAGTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGGTTTTc                                                                                                                                                  >  1:4114272/1‑150 (MQ=255)
       cTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCaa                                                                                                                                                <  1:123343/150‑1 (MQ=255)
       cTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCAGGGTTTTTCa                                                                                                                                                 >  2:682996/1‑150 (MQ=255)
        tttGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCA‑GGTTTTTCAAc                                                                                                                                               >  1:983418/1‑150 (MQ=255)
          tGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCA‑GGGTTTTCAAAGa                                                                                                                                             >  1:1084164/1‑150 (MQ=255)
           gCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGaa                                                                                                                                            >  1:2248078/1‑150 (MQ=255)
             cAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAAtt                                                                                                                                          <  1:3022452/150‑1 (MQ=255)
             cAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAAtt                                                                                                                                          <  1:1827629/150‑1 (MQ=255)
               aaGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGGTTTTCAACGGATTCg                                                                                                                                        >  1:78406/1‑150 (MQ=255)
                   cGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATaa                                                                                                                                    >  1:3239/1‑150 (MQ=255)
                    gTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAAt                                                                                                                                   <  1:645765/150‑1 (MQ=255)
                     tGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAAtt                                                                                                                                  <  2:3058501/150‑1 (MQ=255)
                     tGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAAtt                                                                                                                                  >  1:244237/1‑150 (MQ=255)
                     tGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGAA‑GGTTTTTCAACGAATTCGATAAtt                                                                                                                                  >  2:2979930/1‑150 (MQ=255)
                      ggCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAAttt                                                                                                                                 <  1:4298613/150‑1 (MQ=255)
                         tGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTAt                                                                                                                              <  1:3988624/150‑1 (MQ=255)
                         tGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTAt                                                                                                                              >  1:2955883/1‑150 (MQ=255)
                          ggAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATc                                                                                                                             <  2:1149441/150‑1 (MQ=255)
                           gAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCg                                                                                                                            <  2:3192759/150‑1 (MQ=255)
                             gTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGtt                                                                                                                          <  2:1752847/150‑1 (MQ=255)
                                ctcATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTtctc                                                                                                                       <  1:116443/150‑1 (MQ=255)
                                ctcATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTtctc                                                                                                                       >  1:1381756/1‑150 (MQ=255)
                                 tcATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCtca                                                                                                                      <  2:2956212/150‑1 (MQ=255)
                                  cATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCtcat                                                                                                                     >  1:2647272/1‑150 (MQ=255)
                                    tCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCtcatca                                                                                                                   <  1:1411122/150‑1 (MQ=255)
                                     cgcgATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGGTTTTCAACGAATTCGATAATTTTATCGTTCTCATCaa                                                                                                                  >  1:1271958/1‑150 (MQ=255)
                                        gATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCg                                                                                                               <  1:1619652/150‑1 (MQ=255)
                                         aTCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTTGCA‑GGGTTTTTAACGAATTTGATAATTTTTTCGTTCTCCTCAAccac                                                                                                              >  2:2069086/1‑148 (MQ=255)
                                            gTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCAt                                                                                                           >  1:287282/1‑150 (MQ=255)
                                              cttcttCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGGTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATaa                                                                                                         >  2:3651922/1‑150 (MQ=255)
                                               ttcttcCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAAc                                                                                                        >  1:878249/1‑150 (MQ=255)
                                               ttcttcCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAAc                                                                                                        >  1:3951017/1‑150 (MQ=255)
                                                      agcagTTCATACAGATGGTCGGCGTAAAAGACGTAGATCCCCATCCCCGCAAGAGTTTTGCTCGGATCGTTCGGTATGGGGGGGGGGTTAGCG‑GTTTTTAAAAAAAATTCAATATTTTTATCGTTCTCATCAACCGCCATAACGCCAAAt                                                                                                 <  2:2798789/150‑1 (MQ=255)
                                                          gTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGGTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCCTAACGCCAAAAGCgg                                                                                             >  2:2726357/1‑150 (MQ=255)
                                                            tCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAg                                                                                           >  1:4400321/1‑150 (MQ=255)
                                                            tCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGGTTTTCCACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAg                                                                                           >  2:231845/1‑150 (MQ=255)
                                                            tCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGGTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAg                                                                                           >  1:340631/1‑150 (MQ=255)
                                                              aTACAGATGGTCGGCTAAAAAGACGTAAATCCCCATCCTCGCCAGAGTTTTGCTGGGATCGTCCGGCATGGGCGGGGGGTTAGCG‑GTTTTTACAACAAATTGGATATTTTTTTCTTTCTCATCAACCGCCATAACGCCAAATGCGGAGGc                                                                                         <  2:2359135/150‑1 (MQ=255)
                                                              aTACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGc                                                                                         >  1:4072717/1‑150 (MQ=255)
                                                               tACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGTATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGct                                                                                        >  1:1772423/1‑150 (MQ=255)
                                                                 cAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCCAATGCGGAGGcttc                                                                                      >  1:2090951/1‑150 (MQ=255)
                                                                 cAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGGTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCCAATGCGGAGGcttc                                                                                      >  2:321575/1‑150 (MQ=255)
                                                                 cAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGGTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGcctc                                                                                      >  1:3159005/1‑150 (MQ=255)
                                                                   gATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGcttctt                                                                                    >  1:3741524/1‑150 (MQ=255)
                                                                    aTAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCttcttc                                                                                   >  1:1935625/1‑150 (MQ=255)
                                                                    aTAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAACA‑GGCTTTTCAACAAATTTGATAATTTTATCGTTCTCATCCACCGGCCTAACACCACATTGGGAGGgttcttt                                                                                   >  2:1732933/1‑149 (MQ=255)
                                                                     tAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGGTTTTCAACGAATTCGATAATTTTATTGTTCTCTTCAACCCCCATAACGCCAAATGCGGAGGCttcttcc                                                                                  >  2:2854590/1‑149 (MQ=255)
                                                                     tAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCA‑GGGTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCa                                                                                  >  2:549787/1‑150 (MQ=255)
                                                                        tCGGCGAAAAAGACGTAGACCCCCATCCGCGCGAGATTTTTGCCCGGATCGTGCGGCTTGGGGGGGGGGTGAGCG‑GTTTTTAAAACAAATTCAATATTTTTTTCTTTCCCAACACCCGCCATAACGCCAAATGCGGAGGCTTCTTCAATc                                                                               <  2:3293334/150‑1 (MQ=255)
                                                                         cggcaaaaaaGACGAAGATCCCCATCCCCCCCAGGTTTTTCCCCGGATCTTTCGGTTTTGCGGGGGGGTTAGCT‑GTTTTTCAAACAAATTAAATATTTTTATCTTTCTAATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCg                                                                              <  2:3095614/143‑1 (MQ=255)
                                                                           gcgaaaaaGACGTAGACCCCCATCCCCGCCAGATTTTGGCTCGGTTCGTTCGGCATGGCCGGGGGGTTAGCG‑GTTTTTCCAAAAAATTCGATATTTTTTTCGTTCCCAACAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGt                                                                            <  2:2746631/145‑1 (MQ=255)
                                                                            cGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTa                                                                           >  1:4050415/1‑150 (MQ=255)
                                                                              tCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGCTAGGA‑AGTTTTTCAACGAATTCGATAATTTTTTCGTTCTCATCAACCGCCATAAAACCAAATGCGGAGGCGTCTTTAATTGGtgat                                                                         >  2:1081326/1‑147 (MQ=255)
                                                                                aaaGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGGA‑GGGTTTTTAACGAAATCGATAATTTTATGGGTTTCATCAACCGCCATAACGGCAAAAGCGGAGGGTTATTCAATCGGGACTgg                                                                       >  2:944775/1‑150 (MQ=255)
                                                                                   gccGTAAATACCAATACTCCCGAGATTTTTGCTGGGTTCGTTGGGCATTGGGGGGGGGTTAGCA‑GTTTTTTAAACGAATTGTATATTTTTATCGTTCTCACCACCCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCAt                                                                    <  2:2389943/148‑1 (MQ=255)
                                                                                    aCGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATa                                                                   >  1:2334439/1‑150 (MQ=255)
                                                                                     cGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATAc                                                                  >  1:2692783/1‑150 (MQ=255)
                                                                                      gTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCA‑GGTTTTTTTACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGCGGCTTTTTCAATCGGTACTGGCCTACa                                                                 >  2:754171/1‑150 (MQ=255)
                                                                                      gTAGACACCCATCCCCGCAAGAGATTTGCTCGGATCGTCCGGAATGGGGGGGGGGTTAGCA‑GTTTTTCCAACAAATTCGATATTTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACa                                                                 <  2:4026915/150‑1 (MQ=255)
                                                                                         gacccccATCCCCGCAAGATATTTGCCGGGATCGTTGGGCATGGGGGGGGGGTTGGCG‑GTTTTTCCAACAAATTCAATATTTTTGTCGTTCTCACCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGc                                                              <  2:2260865/146‑1 (MQ=255)
                                                                                          aTACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCa                                                             >  1:3666610/1‑150 (MQ=255)
                                                                                          aTACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCa                                                             >  1:455420/1‑150 (MQ=255)
                                                                                           tACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCaa                                                            >  1:591061/1‑150 (MQ=255)
                                                                                            aCCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAAc                                                           >  1:1331691/1‑150 (MQ=255)
                                                                                            aCCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGGTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAAc                                                           >  1:1546960/1‑150 (MQ=255)
                                                                                                 tACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGGTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGGATACAAGCAACGGGGc                                                      >  1:846064/1‑150 (MQ=255)
                                                                                                 tACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCA‑GGGTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACCGTGc                                                      >  1:3298894/1‑150 (MQ=255)
                                                                                                       ccAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGGTTTTTAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGGGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTg                                                >  2:3367830/1‑150 (MQ=255)
                                                                                                       ccAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGGTTTTTAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGGGGAACGTg                                                >  2:1525364/1‑150 (MQ=255)
                                                                                                         agagATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGGTTTTTAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCCAATGCGGAGGGTTCTTCAATCGGTACTGGCATACAAGCAACGGGGCAACGTgcg                                              >  2:1647326/1‑150 (MQ=255)
                                                                                                            gaTTTGCTCGGATCGTTCGGCATTGACGGCGGGGTAGCA‑GGTTTTTTAACGAATTCGATAATTTTTTCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGGTTCTTTAATCGGTACTGGCATACAAACAACCGTGCAACGTGCGCCt                                           >  2:3621951/1‑150 (MQ=255)
                                                                                                                 gCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTCTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAAAGTGCGCCTTTTTc                                      >  2:1907203/1‑150 (MQ=255)
                                                                                                                  ccggtctCGTTCGGAATTGGCGGGGGGTGGGCA‑TTTTTTTAAACAATTTGAATATTTTTTTCGTTCCCACCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCg                                     <  2:1826838/144‑1 (MQ=255)
                                                                                                                     ggATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACg                                  >  1:1798039/1‑150 (MQ=255)
                                                                                                                      gATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTTTCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTTAATCGGTACTGGCATACAAGCAAAGGTGCAACGTGCGCCTTTTTCGACGt                                 >  2:4234893/1‑150 (MQ=255)
                                                                                                                      gATCGTTCGGCATGGAGGGGGGGTTAGCA‑GTTTTTTAAACAAATTCAATAATTTTATCTTTCTCAACAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGt                                 <  2:2132490/150‑1 (MQ=255)
                                                                                                                        gcGTTCGGCAGTGGCGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGa                               <  2:1214514/149‑1 (MQ=255)
                                                                                                                          gTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATc                             >  2:1782652/1‑150 (MQ=255)
                                                                                                                           ttCGGCATTGACGGCGGGGTAGGA‑GGGGTTTTAAAGAATTCGATAATTTTATCGTTTGGAGCAAACGGCATAACGCCAAATGGGGAGGGTTCTTTAATAGGGACTGGCATACAAGAAAAGGGGCAAAGGGCGGCTTTTTTGACGGGATCg                            >  2:3326573/1‑150 (MQ=255)
                                                                                                                            tCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGa                           >  1:3722568/1‑150 (MQ=255)
                                                                                                                             cGGCATTGACGGCGGGTTAGCA‑GGGTTTTTAACGAATTCGATAATTTTTTCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGAt                          >  2:143751/1‑150 (MQ=255)
                                                                                                                               gCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTTTCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATaa                        >  2:489670/1‑150 (MQ=255)
                                                                                                                                 ttgggcgggggGTTAGAA‑GTTTTTAAAAAAAATTCGAAATTTTTTTCTTTCTCACCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGc                      <  2:4093661/145‑1 (MQ=255)
                                                                                                                                    gggggggggTTAGCA‑TTTTTTTCAACAAATTCGATATTTTTATCGTCCTCACCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATa                   <  2:3911184/144‑1 (MQ=255)
                                                                                                                                    gACGGCGGGTTAGCA‑GGGTTTTCAACGAATTCGATAATTTTATCGGTCTCATCAAACGCCCTAACGCCAAAATCGGAGGCTTCTTCAATCGGTACTGGCATACAAACAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATa                   >  2:1343905/1‑150 (MQ=255)
                                                                                                                                        gcggGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATAcgcg               >  1:149148/1‑150 (MQ=255)
                                                                                                                                           ggTTAGCA‑GTTTTTTCAACGATTTCAATATTTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagt            <  1:2550502/150‑1 (MQ=255)
                                                                                                                                           ggTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagt            >  2:2563562/1‑150 (MQ=255)
                                                                                                                                           ggTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagt            >  1:3520780/1‑150 (MQ=255)
                                                                                                                                            gTTAGCA‑GTTTTTACAACAAATTCAATATTTTTATCTTTCTCACCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagta           <  1:4192974/150‑1 (MQ=255)
                                                                                                                                              tAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGagtagt         >  2:2178956/1‑150 (MQ=255)
                                                                                                                                                gCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTct       >  1:2772629/1‑150 (MQ=255)
                                                                                                                                                 cA‑GTTTTTTCAAGGATTTCGAAAATTTTTCCGTTCTCACCACCCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTctt      <  1:3835946/150‑1 (MQ=255)
                                                                                                                                                 cA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTctt      >  2:1599156/1‑150 (MQ=255)
                                                                                                                                                    ggTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTcttgc    >  1:530555/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
ATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCA‑GGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGGCTTCTTCAATCGGTACTGGCATACAAGCAACGGTGCAACGTGCGCCTTTTTCGACGTGATCGATAAGCATACGCGAGTAGTCTTGCTT  >  AP012306/3020521‑3020819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑