Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,527,3680AC27.0% 689.0 / 43.6 272L140F (TTA→TTC) rhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
Reads supporting (aligned to +/- strand):  ref base A (172/25);  new base C (0/73);  total (172/100)
Fisher's exact test for biased strand distribution p-value = 9.81e-45
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 324 total aligned reads displayed.

CGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTG  >  AP012306/3527219‑3527517
                                                                                                                                                     |                                                                                                                                                     
 gATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTAc                                                                                                                                                      >  1:1120548/1‑150 (MQ=255)
  aTTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTAcc                                                                                                                                                     <  1:2550602/150‑1 (MQ=255)
   tttCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTAccc                                                                                                                                                    >  1:2516870/1‑150 (MQ=255)
   tttCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTAccc                                                                                                                                                    >  2:1155863/1‑150 (MQ=255)
         tttCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGAcc                                                                                                                                              >  1:2687025/1‑150 (MQ=255)
         tttCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGCTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGAcc                                                                                                                                              >  1:319362/1‑150 (MQ=255)
          ttCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCg                                                                                                                                             <  1:3104449/150‑1 (MQ=255)
            ctcGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGcc                                                                                                                                           >  1:443176/1‑150 (MQ=255)
              cGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAg                                                                                                                                         <  2:4233451/150‑1 (MQ=255)
              cGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAg                                                                                                                                         >  2:3771690/1‑150 (MQ=255)
                ccGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTc                                                                                                                                       <  1:2054724/150‑1 (MQ=255)
                 ggggCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTcg                                                                                                                                      <  1:1652538/149‑1 (MQ=255)
                  gggCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTcgc                                                                                                                                     >  1:4084005/1‑150 (MQ=255)
                   ggCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTcgcg                                                                                                                                    >  1:4317756/1‑150 (MQ=255)
                      tGAACCGGTTGCGGCCACAAGAGCTGGAGGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGGGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGAGGGAACAGAAGGAGGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCgaga                                                                                                                                 <  2:4047151/150‑1 (MQ=255)
                       gAATCTTTTGCTGCCACAAGAGCGGGAGGGGCAGTACCCGTCCCCGTGGCGTGTAACCCACGGGGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGAGGGAACGGGGGGAAGGGAAAAATGATTTCCCCCCGACCGCCAGTCGCGAGAt                                                                                                                                <  2:855290/150‑1 (MQ=255)
                       gAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGAt                                                                                                                                >  2:3877149/1‑150 (MQ=255)
                       aaaTCATTCGCTGCCAAAAGAGCGGGATGGGCAGTATCCGTCCCACTGGCGCGTTAACCACAGCGTATTGCAGCGGGGGCGACAGCTGGTTGCACAGATGGAACAGGAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGAt                                                                                                                                <  2:3684219/149‑1 (MQ=255)
                        aaTCGGTTGCTGCCAAAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCCCAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATc                                                                                                                               <  2:607700/150‑1 (MQ=255)
                          tCAGTCGTTGCCAAAGGCGCTGGATGGCGAGAAACCTTCTCCCTGGCGCTTTACCCACGGGTTATTCCGGCGGGGGCGACAGCTGGTTGCAAGGATGGACCAGAGGGAAGGGAAAATTGTTTTCCCCACGGCCGCCAGTCGCGAGATCtt                                                                                                                             <  2:1921391/150‑1 (MQ=255)
                                 ctgcCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATg                                                                                                                      >  2:2896032/1‑150 (MQ=255)
                                  tcccccaAGGGCTGGTTGGGCAGTACCCGTCTCCCTGGCGCGAACACCACGGCTTACTGCGGCGGGTACGCCCGGTGGGTGCAAACAGGTACCCGGAGGAAGGGGAAAATTTTTTCCCCTCGGCCGCCAGTCGCGAGATCTTGTTTATGc                                                                                                                     <  2:1855598/145‑1 (MQ=255)
                                     cacaAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAAt                                                                                                                  >  1:3517504/1‑150 (MQ=255)
                                     cacaAGAGCTGGAGGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGGGTATTGCAGCAGGTGCGACAGCGGGTTGCACAGATGGAACAGCGGGAAGGGAAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAAt                                                                                                                  <  2:1292339/150‑1 (MQ=255)
                                       caAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTa                                                                                                                >  2:2700000/1‑150 (MQ=255)
                                          gagCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTg                                                                                                             >  2:2154305/1‑150 (MQ=255)
                                           agCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGc                                                                                                            >  1:3840086/1‑150 (MQ=255)
                                             cTGGATGGGCAGTACCCGTCTCACTGGCGTTTTACCCACAGCGTATGGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGCCCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGctc                                                                                                          <  2:2280810/150‑1 (MQ=255)
                                              tGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGctct                                                                                                         >  2:1077880/1‑150 (MQ=255)
                                               ggATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCtt                                                                                                        >  1:3457581/1‑150 (MQ=255)
                                                gaggggCAGTATCCGCCCCCCGGGCGCGTAAACCACAGCGTATGGCAGCAGGTCCGCCAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtg                                                                                                       <  2:3719134/147‑1 (MQ=255)
                                                gATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtg                                                                                                       >  2:2567734/1‑150 (MQ=255)
                                                gATGGGAAGTATCCGTCTCACTGGGGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTGGCACGGATGGAACAGAAGGAGGGGAAAAATTTTTTCCCCCCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtg                                                                                                       <  2:3010464/150‑1 (MQ=255)
                                                 aTGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtgc                                                                                                      <  1:3801428/150‑1 (MQ=255)
                                                  tGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCCCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTGttcca                                                                                                     <  2:2475602/150‑4 (MQ=255)
                                                   gggCAGTATCCGTCTCACTGGCGCGTTACCCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGGTGGAACAGCAGGAAGGGAAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtgctg                                                                                                    <  1:1584399/150‑1 (MQ=255)
                                                   gggCAGTACCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGAGGGTGCGACACCGGGTTCCACAGAGGGAACAGCAGGAAGGGAAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtgctg                                                                                                    <  2:3099244/150‑1 (MQ=255)
                                                    ggCAGTACCCGTCTCGATGGCGCGTTAACCACGGCGTATTGCAGCAGGTGCCACAGCTTTTTGCAAAGAGGGACGAGCAGGAGGGGGAAAATGATTCCCCCTCCGCCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtgctgc                                                                                                   <  2:407639/150‑1 (MQ=255)
                                                     gCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCg                                                                                                  >  1:622896/1‑150 (MQ=255)
                                                      cAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGt                                                                                                 >  1:3149207/1‑150 (MQ=255)
                                                       aGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTa                                                                                                >  1:1006566/1‑150 (MQ=255)
                                                        gTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGAAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTaa                                                                                               <  2:3511240/150‑1 (MQ=255)
                                                          aTCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTaaaa                                                                                             >  1:50865/1‑150 (MQ=255)
                                                            ccttctcACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGAGGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGc                                                                                           <  2:2510140/147‑1 (MQ=255)
                                                             cGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCa                                                                                          >  1:2479740/1‑150 (MQ=255)
                                                               tctcCCTGGCGGGTTAACCACAGGGTATTGCAGAAGGTGCGACAGCTGGTTGCACAGATGGAAGAGCAGGAAGGGGAAATTGTTTTACCCTCGCCCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGt                                                                                        <  2:159854/150‑1 (MQ=255)
                                                                ctcACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCACTTACTGCTCTTGCTGCGTAAAAGCAGtt                                                                                       >  1:821747/1‑150 (MQ=255)
                                                                ctcACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGAAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGtt                                                                                       <  1:2775149/150‑1 (MQ=255)
                                                                ctcACGGGCGCGTTAACCACAGCGTATTGCAGAAGGTGCGACAGCTGGTGGCACAGATGGAACAGAAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGtt                                                                                       <  2:1480277/150‑1 (MQ=255)
                                                                  cACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTg                                                                                     >  2:383549/1‑150 (MQ=255)
                                                                    cgggCGCGTTACCCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGAAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCa                                                                                   <  2:2348626/148‑1 (MQ=255)
                                                                     tGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAg                                                                                  <  1:1076092/150‑1 (MQ=255)
                                                                      ggCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTAATGCTCTTGCTGCGTAAAAGCAGTTTGCAgg                                                                                 >  2:1612259/1‑150 (MQ=255)
                                                                        cgctTTACCCACAGCGTATTGCAGCAGGGGCGACGGCTGGTTGCACAGATGGAACAGAAGGAAGGGAAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGgag                                                                               <  2:2821642/150‑1 (MQ=255)
                                                                         gcgTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGgaga                                                                              >  1:2010737/1‑150 (MQ=255)
                                                                            ttAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAAcc                                                                           >  2:4349579/1‑150 (MQ=255)
                                                                            ttAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAAcc                                                                           >  2:209079/1‑150 (MQ=255)
                                                                              aaCCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTg                                                                         >  2:3288267/1‑150 (MQ=255)
                                                                              aaCCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTg                                                                         >  2:676703/1‑150 (MQ=255)
                                                                              aaCCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTg                                                                         >  1:1789545/1‑150 (MQ=255)
                                                                              aaCCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGAAACAGCAGTAAGGGAAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTg                                                                         <  2:2953686/150‑1 (MQ=255)
                                                                               aCCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTgg                                                                        >  1:4192592/1‑150 (MQ=255)
                                                                                ccACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGa                                                                       >  1:3043079/1‑150 (MQ=255)
                                                                                 cacaGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGaa                                                                      <  1:3913547/150‑1 (MQ=255)
                                                                                  acaGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGaaa                                                                     >  1:1422538/1‑150 (MQ=255)
                                                                                  acaGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGaaa                                                                     >  1:2634257/1‑150 (MQ=255)
                                                                                     gCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACa                                                                  >  1:2120650/1‑150 (MQ=255)
                                                                                      gttATGGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGAAAAATGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAg                                                                 <  2:2379009/148‑1 (MQ=255)
                                                                                       gTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAgc                                                                <  1:2895642/150‑1 (MQ=255)
                                                                                       gTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAgc                                                                >  2:4323535/1‑150 (MQ=255)
                                                                                         aTTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAgcgc                                                              >  1:1843806/1‑150 (MQ=255)
                                                                                         aTTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAgcgc                                                              >  1:3477021/1‑150 (MQ=255)
                                                                                           tGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCAt                                                            >  2:3595724/1‑150 (MQ=255)
                                                                                              agcagGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCAc                                                         <  1:3601350/150‑1 (MQ=255)
                                                                                                cagGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGt                                                       >  1:33399/1‑150 (MQ=255)
                                                                                                cagGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGt                                                       >  2:3987623/1‑150 (MQ=255)
                                                                                                    tGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGAAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCa                                                   <  1:4149640/150‑1 (MQ=255)
                                                                                                      cGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAAc                                                 >  1:99784/1‑150 (MQ=255)
                                                                                                        aCAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACtt                                               >  2:4003341/1‑150 (MQ=255)
                                                                                                         cAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTg                                              <  1:1349724/150‑1 (MQ=255)
                                                                                                         cAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTg                                              >  2:2418775/1‑150 (MQ=255)
                                                                                                         cAGCTGGTTGCACAGATGGAACAGCAGAAAGGGGAAAATGTTTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTg                                              <  2:1640824/150‑1 (MQ=255)
                                                                                                           gCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCt                                            <  1:1242358/150‑1 (MQ=255)
                                                                                                           gCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCt                                            <  1:148282/150‑1 (MQ=255)
                                                                                                                     caGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggcctgg                                  >  2:4355196/1‑150 (MQ=255)
                                                                                                                           gAACAGCAGGAAGGGGATAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTggag                            <  1:767074/150‑1 (MQ=255)
                                                                                                                           gAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTggag                            >  2:3163120/1‑150 (MQ=255)
                                                                                                                               agcagGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGAcc                        >  2:3362062/1‑150 (MQ=255)
                                                                                                                               agcagGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTATCAACTTGCTTCTGGCCTGGCTGGAGGAcc                        >  2:4171442/1‑150 (MQ=255)
                                                                                                                                  agGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAtt                     >  1:217120/1‑150 (MQ=255)
                                                                                                                                    gAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAtttt                   >  1:3349711/1‑150 (MQ=255)
                                                                                                                                    gAAGGGGAAAATAATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGTAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAtttt                   <  1:474729/150‑1 (MQ=255)
                                                                                                                                     aaGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTg                  >  2:1410165/1‑150 (MQ=255)
                                                                                                                                         ggAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGa              >  1:1702128/1‑150 (MQ=255)
                                                                                                                                           aaaaTGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATg            >  1:3685503/1‑150 (MQ=255)
                                                                                                                                           aaaaTGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATg            >  1:207100/1‑150 (MQ=255)
                                                                                                                                           aaaaCGATTTCCCCTGGGCCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATg            <  1:695311/150‑1 (MQ=255)
                                                                                                                                                   ttACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAAt    <  1:3795648/150‑1 (MQ=255)
                                                                                                                                                    tACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAAtt   >  2:441675/1‑150 (MQ=255)
                                                                                                                                                     aCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTg  <  2:941197/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTG  >  AP012306/3527219‑3527517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑