BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	392,389	A→C	V325G (GTA→GGA)	acrA ←	multidrug efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	392,389	0	A	C	100.0%	1083.3 / NA	263	V325G (GTA→GGA)	acrA	multidrug efflux system
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (140/123); total (140/123)

Only 100 of 270 total aligned reads displayed.

TTTACCTGGACACCAGGACGCACTTTCTGCAGCCCACTTATTACTACGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTACCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGT  >  AP012306/392242‑392535

tttACCTGGACACCAGGACGCACTTTCTGCAGCCCACTTATTACTCCGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTccc                                                                                                                                                  <  2:77100/150‑1 (MQ=255)

    ccTGGACACCAGGACGCACTTTCTGCAGCCCACTTATTACTACGCGATCGCCTGCTTTCAGACCTTCTGTCCCCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTa                                                                                                                                              <  2:1408286/150‑1 (MQ=255)

    ccTGGACACCAGGACGCACTTTCTGCAGCCCACTTATTACTACGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTa                                                                                                                                              >  1:3463617/1‑150 (MQ=255)

       ggACACCAGGACGCACTTTCTGCAGCCCACTTATTACTACGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACgg                                                                                                                                           <  2:531420/150‑1 (MQ=255)

         acacCAGGACGCACTTTCTGCAGCCCACTTATTACTACGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTg                                                                                                                                         <  1:1001696/150‑1 (MQ=255)

          cacCAGGACGCAATTTCTGCAGCCCACTTATTACTACGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTgg                                                                                                                                        >  2:4377432/1‑150 (MQ=255)

             cAGGACGCACTTTCTGCAGCCCACTTATTACTACGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACCCCCAGTACGGTGGCAt                                                                                                                                     <  1:3050485/150‑1 (MQ=255)

                gACGCCCTTTCTGCAGCCCACTTATTACTACGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGc                                                                                                                                  <  2:3164918/150‑1 (MQ=255)

                 aCGCACTTTCTGCAGCCCACTTATTACTACGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGcc                                                                                                                                 >  2:3130344/1‑150 (MQ=255)

                 aCGCACTTTCTGCAGCCCACTTATTACTACGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGcc                                                                                                                                 >  1:2947297/1‑150 (MQ=255)

                 aCGCACTTTCTGCAGCCCACTTATTACTACGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGcc                                                                                                                                 >  2:3048663/1‑150 (MQ=255)

                 aCGCACTTTCTGCAGCCCACTTATTACTACGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGcc                                                                                                                                 >  2:3240022/1‑150 (MQ=255)

                    cACTTTCTGCAGCCCCCTTATTACTACGCGATCGCCTGCTTTCAGACCTTCTGTCCCCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCAcg                                                                                                                              <  2:1814813/150‑1 (MQ=255)

                     aCTTTCTGCAGCCCACTTATTACTACGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCAcgc                                                                                                                             >  2:403052/1‑150 (MQ=255)

                     aCTTTCTGCAGCCCACTTATTACTACGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCAcgc                                                                                                                             >  1:2446461/1‑150 (MQ=255)

                       tttCTGCAGCCCACTTATTACTACGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACgcgg                                                                                                                           >  1:481986/1‑150 (MQ=255)

                        ttCTGCAGCCCACTTATTACTACGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACgcggc                                                                                                                          >  1:3945096/1‑150 (MQ=255)

                           tGCAGCCCACTTATTACTACGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTa                                                                                                                       <  2:1671929/150‑1 (MQ=255)

                             cAGCCCACTTATTACTACGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACg                                                                                                                     <  1:1441836/150‑1 (MQ=255)

                             cAGCCCACTTAGTACTACGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACg                                                                                                                     <  1:683332/150‑1 (MQ=255)

                                 ccACTTATTACTACGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGtt                                                                                                                 <  1:2890093/150‑1 (MQ=255)

                                   aCTTATTACTACGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTAc                                                                                                               >  2:3962131/1‑150 (MQ=255)

                                   aCTTATTACTACGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTAc                                                                                                               <  2:4251574/150‑1 (MQ=255)

                                    cTTATTACTACGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACg                                                                                                              >  1:1301639/1‑150 (MQ=255)

                                     ttattaCTACGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGc                                                                                                             >  2:2264304/1‑150 (MQ=255)

                                      tattaCTACGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGcc                                                                                                            >  1:890748/1‑150 (MQ=255)

                                       attaCTACGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGccc                                                                                                           <  2:3885060/150‑1 (MQ=255)

                                         tactacGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTg                                                                                                         >  1:1667874/1‑150 (MQ=255)

                                          actacGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGt                                                                                                        >  2:4332633/1‑150 (MQ=255)

                                           ctacGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGtt                                                                                                       <  2:339693/150‑1 (MQ=255)

                                           ctacGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGtt                                                                                                       <  1:4046546/150‑1 (MQ=255)

                                            tacGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTg                                                                                                      >  2:3563597/1‑150 (MQ=255)

                                             acGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGc                                                                                                     >  1:2575791/1‑150 (MQ=255)

                                              cgcgATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCg                                                                                                    >  1:1888715/1‑150 (MQ=255)

                                               gcgATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCgg                                                                                                   <  1:2433034/150‑1 (MQ=255)

                                                      ccTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTaaa                                                                                            <  1:4241520/150‑1 (MQ=255)

                                                       cTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTaaaa                                                                                           <  2:3451028/150‑1 (MQ=255)

                                                        tGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAAt                                                                                          <  1:2261047/150‑1 (MQ=255)

                                                          cTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAg                                                                                        <  1:1153584/150‑1 (MQ=255)

                                                             tCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGt                                                                                     <  2:905628/150‑1 (MQ=255)

                                                              cAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGtt                                                                                    >  2:1431774/1‑150 (MQ=255)

                                                               aGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGttt                                                                                   >  2:2086127/1‑150 (MQ=255)

                                                                gACCTTCTGTCCCCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTg                                                                                  <  2:3032756/150‑1 (MQ=255)

                                                                gACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTg                                                                                  <  2:2887033/150‑1 (MQ=255)

                                                                gACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTg                                                                                  >  2:1760681/1‑150 (MQ=255)

                                                                   cTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGAt                                                                               >  1:2883733/1‑150 (MQ=255)

                                                                    ttCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGAtt                                                                              <  1:1313241/150‑1 (MQ=255)

                                                                     tCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTa                                                                             <  2:4222845/150‑1 (MQ=255)

                                                                      cTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTaa                                                                            <  1:275311/150‑1 (MQ=255)

                                                                       tGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAg                                                                           >  2:2338037/1‑150 (MQ=255)

                                                                       tGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAg                                                                           <  1:2419952/150‑1 (MQ=255)

                                                                       tGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAg                                                                           >  1:3613772/1‑150 (MQ=255)

                                                                       tGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAg                                                                           >  2:4398737/1‑150 (MQ=255)

                                                                         tCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGcc                                                                         >  1:4194661/1‑150 (MQ=255)

                                                                           aCCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCct                                                                       <  1:976899/150‑1 (MQ=255)

                                                                           aCCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCct                                                                       <  1:4302694/150‑1 (MQ=255)

                                                                             cAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCcttc                                                                     >  2:1678563/1‑150 (MQ=255)

                                                                              aGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCcttct                                                                    >  1:3204171/1‑150 (MQ=255)

                                                                              aGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCcttct                                                                    <  2:63860/150‑1 (MQ=255)

                                                                               gCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCcttctt                                                                   >  2:3681010/1‑150 (MQ=255)

                                                                               gCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCcttctt                                                                   >  1:2713304/1‑150 (MQ=255)

                                                                                 cACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTcc                                                                 <  2:1872319/150‑1 (MQ=255)

                                                                                 cACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTcc                                                                 >  1:151820/1‑150 (MQ=255)

                                                                                  aCTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCa                                                                >  1:185745/1‑150 (MQ=255)

                                                                                    ttATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGTGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGa                                                              <  1:3788897/150‑1 (MQ=255)

                                                                                    ttATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGa                                                              <  1:228276/150‑1 (MQ=255)

                                                                                     tATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGAc                                                             <  1:2016717/150‑1 (MQ=255)

                                                                                     tATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGAc                                                             <  1:3832878/150‑1 (MQ=255)

                                                                                      aTCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACg                                                            <  2:1737891/150‑1 (MQ=255)

                                                                                      aTCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACg                                                            >  2:1057755/1‑150 (MQ=255)

                                                                                         gCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTgc                                                         <  1:958695/150‑1 (MQ=255)

                                                                                              tAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTGCGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCa                                                    <  1:568945/150‑1 (MQ=255)

                                                                                               aGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCAc                                                   <  1:2830757/150‑1 (MQ=255)

                                                                                                   tGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAAc                                               <  1:1056186/150‑1 (MQ=255)

                                                                                                   tGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAAc                                               <  2:1119914/150‑1 (MQ=255)

                                                                                                       ttGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATAc                                           >  2:681445/1‑150 (MQ=255)

                                                                                                         gCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATAccc                                         >  1:3510312/1‑150 (MQ=255)

                                                                                                          cAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCg                                        >  1:758070/1‑150 (MQ=255)

                                                                                                          cAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCg                                        <  2:3751857/150‑1 (MQ=255)

                                                                                                           aaCGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCgg                                       >  1:1506151/1‑150 (MQ=255)

                                                                                                            aCGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGgc                                      <  2:1278701/150‑1 (MQ=255)

                                                                                                            aCGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGgc                                      >  2:2796750/1‑150 (MQ=255)

                                                                                                             cGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGgca                                     >  2:718883/1‑150 (MQ=255)

                                                                                                                  ggACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCaga                                >  2:2823032/1‑150 (MQ=255)

                                                                                                                  ggACGGGTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCaga                                >  2:2174700/1‑150 (MQ=255)

                                                                                                                        gTTTCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGa                          <  1:41545/150‑1 (MQ=255)

                                                                                                                         tttCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACTCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGTCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCATTGTGTGag                         >  2:29723/1‑149 (MQ=255)

                                                                                                                         tttCCACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGAt                         <  2:1372450/150‑1 (MQ=255)

                                                                                                                             cACTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCgg                     >  1:1720790/1‑150 (MQ=255)

                                                                                                                              aCTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCggg                    <  2:3484908/150‑1 (MQ=255)

                                                                                                                               cTTTGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGt                   <  2:1584218/150‑1 (MQ=255)

                                                                                                                                tttGTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGtt                  >  1:3386800/1‑150 (MQ=255)

                                                                                                                                   gTCATCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCgg               >  1:2660249/1‑150 (MQ=255)

                                                                                                                                      aTCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGaa            >  2:2516231/1‑150 (MQ=255)

                                                                                                                                       tCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAg           <  2:1477662/150‑1 (MQ=255)

                                                                                                                                       tCCGCGCCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAg           >  1:3044396/1‑150 (MQ=255)

                                                                                                                                           cgcCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAg       <  2:1975846/150‑1 (MQ=255)

                                                                                                                                            gcCAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAgc      >  2:852206/1‑150 (MQ=255)

                                                                                                                                              cAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAgcgc    >  2:2033074/1‑150 (MQ=255)

                                                                                                                                              cAACTCCCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAgcgc    <  2:1842719/150‑1 (MQ=255)

TTTACCTGGACACCAGGACGCACTTTCTGCAGCCCACTTATTACTACGCGATCGCCTGCTTTCAGACCTTCTGTCACCAGCCACTTATCGCCAATAGCCTGGCTTGCAACGATCGGACGGGTTTCCACTTTGTCATCCGCGCCAACTACCAGTACGGTGGCATCGCCACGCGGCGTACGGGTTACGCCCTGTTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGT  >  AP012306/392242‑392535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`