Predicted mutation
evidence seq id position mutation annotation gene description
RA AP012306 573,790 G→T P46T (CCC→ACC)  nagC ← DNA‑binding transcriptional dual regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306573,7900GT99.6% 1004.8 / NA 265P46T (CCC→ACC) nagCDNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (136/128);  minor base A (1/0);  total (137/128)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 283 total aligned reads displayed.

CCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTA  >  AP012306/573645‑573936
                                                                                                                                                 |                                                                                                                                                  
ccAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGGGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGGAATTTTGGTTACGCTGGCGGTGGCa                                                                                                                                                >  2:810746/1‑150 (MQ=255)
  aaGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAg                                                                                                                                              >  1:2136927/1‑150 (MQ=255)
  aaGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAg                                                                                                                                              >  1:1584600/1‑150 (MQ=255)
    gCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCt                                                                                                                                            >  2:1863542/1‑150 (MQ=255)
     ccGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTg                                                                                                                                           >  1:1931649/1‑150 (MQ=255)
        tACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCt                                                                                                                                        >  2:196515/1‑150 (MQ=255)
         aCGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCtt                                                                                                                                       <  2:5490/150‑1 (MQ=255)
          cGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCttt                                                                                                                                      <  1:2820131/150‑1 (MQ=255)
          cGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGAAAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCttt                                                                                                                                      >  2:2334573/1‑150 (MQ=255)
            ccGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGc                                                                                                                                    >  2:4190135/1‑150 (MQ=255)
             cGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCt                                                                                                                                   <  2:1291202/150‑1 (MQ=255)
               aTTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCg                                                                                                                                 <  2:1454487/150‑1 (MQ=255)
                  gCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCa                                                                                                                              <  2:2645166/150‑1 (MQ=255)
                   cGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCaa                                                                                                                             >  1:2808117/1‑150 (MQ=255)
                        aaaTTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGa                                                                                                                        >  1:4358977/1‑150 (MQ=255)
                           ttGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATc                                                                                                                     <  2:3961086/150‑1 (MQ=255)
                              cGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGCTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCcgc                                                                                                                  >  1:133905/1‑150 (MQ=255)
                                ggTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCga                                                                                                                <  1:2271088/150‑1 (MQ=255)
                                ggTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCga                                                                                                                >  1:1682308/1‑150 (MQ=255)
                                      ggTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTgg                                                                                                          >  1:3034533/1‑150 (MQ=255)
                                      ggTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTgg                                                                                                          <  2:3662999/150‑1 (MQ=255)
                                          aCGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCg                                                                                                      >  1:3792029/1‑150 (MQ=255)
                                            gATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAAATTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTa                                                                                                    >  2:16971/1‑150 (MQ=255)
                                                gagaTAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTgg                                                                                                >  1:1453910/1‑150 (MQ=255)
                                                 agaTAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGt                                                                                               >  2:2785259/1‑150 (MQ=255)
                                                  gaTAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTc                                                                                              <  1:3599444/150‑1 (MQ=255)
                                                       cgcgGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCa                                                                                         >  1:525277/1‑150 (MQ=255)
                                                        gcggcgGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGGACTGGTCAATCAg                                                                                        >  1:2299139/1‑150 (MQ=255)
                                                         cggcggCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAgg                                                                                       <  1:1956591/150‑1 (MQ=255)
                                                          ggcggcCCCCGGTGGAGGCTTGCTGACCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGACTCCGCGAGATTGGCCCGTACTGGTCAATCAGGc                                                                                      <  2:181626/150‑1 (MQ=255)
                                                          ggcggcCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGc                                                                                      >  2:1733651/1‑150 (MQ=255)
                                                             ggcCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGAt                                                                                   <  2:3481724/150‑1 (MQ=255)
                                                              gcCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATa                                                                                  <  1:2618130/150‑1 (MQ=255)
                                                               cccccGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATaa                                                                                 <  1:1198035/150‑1 (MQ=255)
                                                                 cccGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAAc                                                                               >  2:332933/1‑150 (MQ=255)
                                                                  ccGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATTAGGCGATAAAcc                                                                              >  1:2224029/1‑150 (MQ=255)
                                                                     gTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAAccgcc                                                                           <  1:1810989/150‑1 (MQ=255)
                                                                       ggAGCCCTGCTGATAACCTTTTTTGATCGGCCCGCGTTGGATAAGCTGAGGGGTAATTTTGTTTGCGCGGGGGGGGGAAGGCTGGCTTTGCTCGGCAATCTGAACCCGCGAGATTGGCCGGTACTGGTCAATCGGGGGATAAACcgccgc                                                                         <  2:369461/150‑1 (MQ=255)
                                                                           gCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTg                                                                     <  2:4084437/150‑1 (MQ=255)
                                                                            ccTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGt                                                                    >  1:3150449/1‑150 (MQ=255)
                                                                            ccTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGt                                                                    >  2:176173/1‑150 (MQ=255)
                                                                             ctgctgATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGtt                                                                   >  1:199505/1‑150 (MQ=255)
                                                                                ctgATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAg                                                                >  1:3558411/1‑150 (MQ=255)
                                                                                  gATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCt                                                              >  2:1542405/1‑150 (MQ=255)
                                                                                     cAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGtt                                                           >  2:1489612/1‑150 (MQ=255)
                                                                                      aaCTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGttt                                                          >  1:1852491/1‑150 (MQ=255)
                                                                                       aCTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGtttt                                                         >  1:3378554/1‑150 (MQ=255)
                                                                                       aCTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGtttt                                                         <  1:808608/150‑1 (MQ=255)
                                                                                        ctttttTGATCGGCCGGCGTTCGATAAGCGGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTa                                                        <  2:3202765/150‑1 (MQ=255)
                                                                                            tttGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACgag                                                    >  1:4225559/1‑150 (MQ=255)
                                                                                            tttGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACgag                                                    <  1:3389818/150‑1 (MQ=255)
                                                                                               gATCGGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATc                                                 <  1:876012/150‑1 (MQ=255)
                                                                                               gATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATc                                                 >  1:1523222/1‑150 (MQ=255)
                                                                                                 tcggCCCGCGTTCGATAAGTTGACGGGTAATTTTGGTTACGCGGGCGGTGGCAGGCTGGCTTTACTCGGCAATCTGAATCCGCGGGATTGGCCGTTCCTGGGAAATCAGGGGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCaa                                               <  2:1685185/147‑1 (MQ=255)
                                                                                                 tCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCaa                                               <  1:3170089/150‑1 (MQ=255)
                                                                                                 tCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCaa                                               <  2:2921823/150‑1 (MQ=255)
                                                                                                  cAGCCCGGGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAAc                                              <  1:1117168/150‑1 (MQ=255)
                                                                                                  cAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAAc                                              <  1:3475624/150‑1 (MQ=255)
                                                                                                   aGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGAGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACa                                             >  2:140023/1‑150 (MQ=255)
                                                                                                    gCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACAt                                            <  2:959286/150‑1 (MQ=255)
                                                                                                    gCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACAt                                            <  1:1286145/150‑1 (MQ=255)
                                                                                                    gCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACAt                                            >  2:245823/1‑150 (MQ=255)
                                                                                                     cccGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACAtt                                           >  1:974727/1‑150 (MQ=255)
                                                                                                     cccGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACAtt                                           <  2:3076935/150‑1 (MQ=255)
                                                                                                     cccGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACAtt                                           <  2:2728582/150‑1 (MQ=255)
                                                                                                       cgcgTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTAc                                         <  1:1079880/150‑1 (MQ=255)
                                                                                                           ttCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACgaga                                                   >  2:2983391/1‑136 (MQ=255)
                                                                                                             cGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCt                                   >  2:1988833/1‑150 (MQ=255)
                                                                                                              gATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTg                                  >  1:2970012/1‑150 (MQ=255)
                                                                                                              gATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTg                                  >  2:4033985/1‑150 (MQ=255)
                                                                                                                tAAGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTGGGCAATCTGACTCCGCGAGATTGGCCCGTACTGGTCAACCAGGCGATAACCCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAg                                <  2:1904997/150‑1 (MQ=255)
                                                                                                                 aaGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGc                               >  2:312242/1‑150 (MQ=255)
                                                                                                                 aaGCTGACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGc                               <  2:2834184/150‑1 (MQ=255)
                                                                                                                      gACGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTc                          >  1:3095469/1‑150 (MQ=255)
                                                                                                                       aCGCGTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTcc                         <  1:3726383/150‑1 (MQ=255)
                                                                                                                          cgTAATTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGcc                      >  1:2244517/1‑150 (MQ=255)
                                                                                                                           gTAATTTTGGTTCCGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCt                     <  2:1174240/150‑1 (MQ=255)
                                                                                                                             aaTTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTGGGCAATCTGAATCCGCGAGATTGGCCGGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTgg                   <  1:3268534/150‑1 (MQ=255)
                                                                                                                             aaTTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTgg                   <  1:2889622/150‑1 (MQ=255)
                                                                                                                             aaTTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTgg                   >  1:703624/1‑150 (MQ=255)
                                                                                                                             aaTTTTGGTTACGCTGGCGGTGGCAAACTGCCTTTGCTCGGCAATCTGAATCCGCCAGATTGGCCCGGACTTGTTCATCAGGCGATAAACCGCCGCCCTGTTAAACTGATTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTgg                   >  2:3463352/1‑150 (MQ=255)
                                                                                                                              aTTTTGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGgt                  >  2:496130/1‑150 (MQ=255)
                                                                                                                                 ttGGTTACGCTGGGGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTc               <  2:437865/150‑1 (MQ=255)
                                                                                                                                 ttGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTc               <  1:2671379/150‑1 (MQ=255)
                                                                                                                                  tGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCa              <  2:301574/150‑1 (MQ=255)
                                                                                                                                  tGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCa              <  2:988835/150‑1 (MQ=255)
                                                                                                                                  tGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCa              >  1:4080305/1‑150 (MQ=255)
                                                                                                                                  tGGTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGGCAATCAGGCGATAAACCGGCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGTGCTTGTCCGCCTGGTGTCa              >  2:2663973/1‑150 (MQ=255)
                                                                                                                                   ggTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCAt             <  1:3316216/150‑1 (MQ=255)
                                                                                                                                    gTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATa            <  2:2144190/150‑1 (MQ=255)
                                                                                                                                    gTTACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATa            <  2:696138/150‑1 (MQ=255)
                                                                                                                                     ttACGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGGACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATAc           >  2:398801/1‑150 (MQ=255)
                                                                                                                                       aCGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCCATTAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTTTCCGCCTGGTGCCATACtt         >  2:2570823/1‑150 (MQ=255)
                                                                                                                                       aCGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACtt         >  1:2445800/1‑150 (MQ=255)
                                                                                                                                       aCGCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACtt         >  1:4255614/1‑150 (MQ=255)
                                                                                                                                         gCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTtc       <  1:2060854/150‑1 (MQ=255)
                                                                                                                                         gCTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCCTACTGGTCAAACAGGCGATAAAACCCCGCGCCGTTAAACTGTTTTACGAGATCAACATTACCTATCTGAACCTGTCCCCCTTGTGGCCTACtttt       >  2:2117775/1‑149 (MQ=255)
                                                                                                                                          cTGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTtct      <  2:2245387/150‑1 (MQ=255)
                                                                                                                                           tGGCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTtctc     >  2:843778/1‑150 (MQ=255)
                                                                                                                                             gCGGTGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCtt   <  2:3862798/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTTACGAGATCAACATTACCTATCTGAGCTTGTCCGCCTGGTGTCATACTTTCTCTTA  >  AP012306/573645‑573936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑