Predicted mutation
evidence seq id position mutation annotation gene description
RA AP012306 612,924 C→A R660L (CGC→CTC)  ybgQ ← predicted outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306612,9240CA99.7% 1234.5 / ‑3.1 310R660L (CGC→CTC) ybgQpredicted outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (117/192);  total (118/192)
Fisher's exact test for biased strand distribution p-value = 3.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.81e-01

Only 100 of 318 total aligned reads displayed.

CGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGCGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGAAATATCCCACTCACCCGCTGAACTCAGGTGC  >  AP012306/612778‑613070
                                                                                                                                                  |                                                                                                                                                  
cGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTg                                                                                                                                                 <  1:573745/150‑1 (MQ=255)
 gTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTgg                                                                                                                                                >  2:3454357/1‑150 (MQ=255)
 gTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTgg                                                                                                                                                >  1:2945297/1‑150 (MQ=255)
       tAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCAtt                                                                                                                                          <  1:3676500/150‑1 (MQ=255)
        aaGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATtg                                                                                                                                         <  1:3167352/150‑1 (MQ=255)
        aaGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATtg                                                                                                                                         >  1:1670723/1‑150 (MQ=255)
         aGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATtgg                                                                                                                                        <  1:1191005/150‑1 (MQ=255)
             attcatGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGa                                                                                                                                    <  2:1793449/150‑1 (MQ=255)
             attcatGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGa                                                                                                                                    <  1:2179717/150‑1 (MQ=255)
               tcatGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGc                                                                                                                                  <  1:3315132/150‑1 (MQ=255)
                catGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCt                                                                                                                                 <  2:2461648/150‑1 (MQ=255)
                     tCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGCACCACCGCAATGCCGAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTgcggc                                                                                                                            <  1:4114649/150‑1 (MQ=255)
                      cACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTgcggcg                                                                                                                           <  1:1129077/150‑1 (MQ=255)
                           ccACGGTGGAAGGCTGGTAACTGGAAATCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGaa                                                                                                                      >  1:1793880/1‑150 (MQ=255)
                            cACGGTGGAAGGCTGGTAACTGGAAATCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGaaa                                                                                                                     <  2:668741/150‑1 (MQ=255)
                             aCGGTGGAAGGCTGGTAACTGGAAATCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAAt                                                                                                                    <  2:1577710/150‑1 (MQ=255)
                             aCGGTGGAAGGCTGGTAACTGGAAATCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAAt                                                                                                                    <  2:4327677/150‑1 (MQ=255)
                                  ggAAGGCTGGTAACTGGAAATCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAAtgctgc                                                                                                               <  1:2597444/150‑1 (MQ=255)
                                     aGGCTGGTAACTGGAAATCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCAcc                                                                                                            >  2:2278165/1‑150 (MQ=255)
                                           gTAACTGGAAATCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTg                                                                                                      >  1:2358365/1‑150 (MQ=255)
                                            tAACTGGAAATCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTgg                                                                                                     >  1:639877/1‑150 (MQ=255)
                                             aaCTGGAAATCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTggg                                                                                                    >  1:3186819/1‑150 (MQ=255)
                                              aCTGGAAATCAACGGCACCACCGCAATGTCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGt                                                                                                   <  1:622388/150‑1 (MQ=255)
                                               cTGGAAATCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGtt                                                                                                  >  1:3972638/1‑150 (MQ=255)
                                               cTGGAAATCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGtt                                                                                                  >  1:1614575/1‑150 (MQ=255)
                                                 ggAAATCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGc                                                                                                >  2:3968331/1‑150 (MQ=255)
                                                  gAAATCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCg                                                                                               <  2:2242595/150‑1 (MQ=255)
                                                   aaaTCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCgg                                                                                              <  1:1435027/150‑1 (MQ=255)
                                                   aaaTCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCgg                                                                                              >  1:4260302/1‑150 (MQ=255)
                                                       cAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGaa                                                                                          <  1:3829075/150‑1 (MQ=255)
                                                            gCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAAc                                                                                     >  1:1015554/1‑150 (MQ=255)
                                                             caccacCGCACTGGCGAAAGGGTTGGTGTAGTCGAGATTGCCGTGGACCGGAATATCTGCCACGGCACCGGTGCTGGCCATCACGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATTCTGCACCATATTGGGTTGCGGTGAAAGAGcc                                                                                    <  2:2839845/150‑1 (MQ=255)
                                                             caccacCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGATGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAAcc                                                                                    >  2:1234009/1‑150 (MQ=255)
                                                                 accGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTc                                                                                <  1:2762125/150‑1 (MQ=255)
                                                                  ccGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTcc                                                                               <  1:4034201/150‑1 (MQ=255)
                                                                   cGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCa                                                                              <  2:2570207/150‑1 (MQ=255)
                                                                      aaTGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCACCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAgct                                                                           <  2:527040/150‑1 (MQ=255)
                                                                         gCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAgctgct                                                                        <  1:1834257/150‑1 (MQ=255)
                                                                          ccAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAgctgctg                                                                       <  2:2016994/150‑1 (MQ=255)
                                                                           cAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAgctgctgc                                                                      <  1:2028537/150‑1 (MQ=255)
                                                                            aaaaTGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAgctgctgct                                                                     >  2:249207/1‑150 (MQ=255)
                                                                             aaaTGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGctgctgctg                                                                    >  1:1145331/1‑150 (MQ=255)
                                                                             aaaTGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGctgctgctg                                                                    >  2:1695271/1‑150 (MQ=255)
                                                                              aaTGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGa                                                                   >  2:2542815/1‑150 (MQ=255)
                                                                               aTGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGAc                                                                  <  2:2038721/150‑1 (MQ=255)
                                                                                tggttggtGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACg                                                                 >  2:4085692/1‑150 (MQ=255)
                                                                                tggttggtGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACg                                                                 <  1:147931/150‑1 (MQ=255)
                                                                                 ggttggtGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACgg                                                                >  1:2277658/1‑150 (MQ=255)
                                                                                 ggttggtGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACgg                                                                >  1:3741104/1‑150 (MQ=255)
                                                                                 ggttggtGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACgg                                                                <  2:4394057/150‑1 (MQ=255)
                                                                                  gttggtGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGa                                                               >  2:318203/1‑150 (MQ=255)
                                                                                  gttggtGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGa                                                               >  1:129090/1‑150 (MQ=255)
                                                                                      gtgtAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTg                                                           >  1:3468588/1‑150 (MQ=255)
                                                                                       tgtAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGt                                                          <  2:1442914/150‑1 (MQ=255)
                                                                                          aGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAAt                                                       <  1:2558572/150‑1 (MQ=255)
                                                                                           gTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATc                                                      <  2:4157166/150‑1 (MQ=255)
                                                                                            tCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCa                                                     <  2:907201/150‑1 (MQ=255)
                                                                                             cGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCAc                                                    >  1:3044000/1‑150 (MQ=255)
                                                                                               agaTTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTg                                                  >  1:472609/1‑150 (MQ=255)
                                                                                                gaTTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTgg                                                 >  1:133056/1‑150 (MQ=255)
                                                                                                 aTTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTggc                                                <  1:3928919/150‑1 (MQ=255)
                                                                                                 aTTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTggc                                                <  2:718643/150‑1 (MQ=255)
                                                                                                 aTTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGGGGGTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTggc                                                >  2:355141/1‑150 (MQ=255)
                                                                                                       cTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATa                                          <  2:1912574/150‑1 (MQ=255)
                                                                                                        tGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAg                                         <  1:906887/150‑1 (MQ=255)
                                                                                                        tGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAg                                         <  1:2450933/150‑1 (MQ=255)
                                                                                                         gAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAgg                                        >  2:2910140/1‑150 (MQ=255)
                                                                                                           aCCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTa                                      <  1:2986749/150‑1 (MQ=255)
                                                                                                            ccGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTAc                                     >  2:2440534/1‑150 (MQ=255)
                                                                                                            ccGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTAc                                     <  2:3267266/150‑1 (MQ=255)
                                                                                                             cGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTAcc                                    >  2:1743884/1‑150 (MQ=255)
                                                                                                                 atatCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGaa                                <  1:4392017/150‑1 (MQ=255)
                                                                                                                 atatCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGaa                                <  2:2380726/150‑1 (MQ=255)
                                                                                                                 atatCTGCCACGCCATCGGTGCTGACCATCAGGAGGGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGGACCAGaa                                >  1:71484/1‑150 (MQ=255)
                                                                                                                  tatCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGaaa                               <  2:2141437/150‑1 (MQ=255)
                                                                                                                  tatCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGaaa                               <  2:1962450/150‑1 (MQ=255)
                                                                                                                   atCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGAAat                              <  2:3598486/150‑1 (MQ=255)
                                                                                                                    tCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGAAata                             <  1:1859671/150‑1 (MQ=255)
                                                                                                                    tCTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGAAata                             <  1:4291089/150‑1 (MQ=255)
                                                                                                                     cTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGAAatat                            <  2:3502482/150‑1 (MQ=255)
                                                                                                                     cTGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGAAatat                            <  1:1046089/150‑1 (MQ=255)
                                                                                                                      tGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGAAATATc                           <  1:796134/150‑1 (MQ=255)
                                                                                                                      tGCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGAAATATc                           >  2:1334419/1‑150 (MQ=255)
                                                                                                                       gCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGAAATATcc                          >  1:1877100/1‑150 (MQ=255)
                                                                                                                       gCCACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGAAATATcc                          >  2:3117500/1‑150 (MQ=255)
                                                                                                                        ccACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGAAATATccc                         >  2:1825493/1‑150 (MQ=255)
                                                                                                                         cACGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGAAATATCCCa                        <  2:773443/150‑1 (MQ=255)
                                                                                                                           cGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGAAATATCCCACt                      <  2:2780586/150‑1 (MQ=255)
                                                                                                                           cGCCATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGAAATATCCCACt                      <  1:4359399/150‑1 (MQ=255)
                                                                                                                             ccATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGAAATATCCCACTCa                    >  2:1299188/1‑150 (MQ=255)
                                                                                                                             ccATCGGTGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGAAATATCCCACTCa                    <  2:426949/150‑1 (MQ=255)
                                                                                                                                  ggTGCTGACCATCAGGCGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGAAATATCCCACTCACCCGc               >  1:1034700/1‑150 (MQ=255)
                                                                                                                                    tGCTGACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGAAATATCCCACTCACCCGCTg             <  2:1110610/150‑1 (MQ=255)
                                                                                                                                        gACCATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGAAATATCCCACTCACCCGCTGAACt         <  1:2302182/150‑1 (MQ=255)
                                                                                                                                         tccATCAGCTGTGGCTCATTGGGGAAGCTGCGGCGGTGAAATGCTGCACCCTATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGGAATTACTGGCGGTTTAGGTACCAGAAATATCCCACTCACCCGCTGaaacc        >  2:2719256/2‑147 (MQ=255)
                                                                                                                                           cATCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGAAATATCCCACTCACCCGCTGAACTCAg      <  1:2148354/150‑1 (MQ=255)
                                                                                                                                            aTCAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGAAATATCCCACTCACCCGCTGAACTCAgg     >  2:2957877/1‑150 (MQ=255)
                                                                                                                                              cAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTTACGGAACTGTAATCACTGGCGGCATAGGTACCAGAAATATCCCACTCACCCGCTGAACTCAGGTg   <  1:1771018/150‑1 (MQ=255)
                                                                                                                                              cAGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGAAATATCCCACTCACCCGCTGAACTCAGGTg   >  2:3671518/1‑150 (MQ=255)
                                                                                                                                               aGGAGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGAAATATCCCACTCACCCGCTGAACTCAGGTGc  >  2:2364123/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAATCAACGGCACCACCGCAATGCCAAAATGGTTGGTGTAGTCGAGATTGCCCTGAACCGGAATATCTGCCACGCCATCGGTGCTGACCATCAGGCGTGGTTCATTGGTGGAGCTGCGGCGATGAAATGCTGCACCATATTGGGTTGCGGTGAAAGAACCGCTCCAGCTGCTGCTGACGGAACTGTAATCACTGGCGGCATAGGTACCAGAAATATCCCACTCACCCGCTGAACTCAGGTGC  >  AP012306/612778‑613070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑