BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	574,224	+T	coding (859/1149 nt)	nagA ←	N‑acetylglucosamine‑6‑phosphate deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	574,224	1	.	T	100.0%	1418.8 / NA	350	F287I (TTT→ATT)	nagA	N‑acetylglucosamine‑6‑phosphate deacetylase
Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (157/193); total (157/193)

Only 100 of 372 total aligned reads displayed.

CGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAA‑AATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAG  >  AP012306/574075‑574367

cGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCaaa                                                                                                                                                  <  1:3075155/150‑1 (MQ=255)

 gTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTGAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCaaat                                                                                                                                                 <  1:1156978/150‑2 (MQ=255)

  tCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCaaata                                                                                                                                                <  2:2784213/150‑3 (MQ=255)

   cGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCaaataa                                                                                                                                               >  1:955574/1‑147 (MQ=255)

     ccATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCaaataatg                                                                                                                                             <  1:569062/150‑6 (MQ=255)

      cATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGa                                                                                                                                            >  1:1335569/1‑150 (MQ=255)

       aTACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGaa                                                                                                                                           >  1:4061342/1‑150 (MQ=255)

         aCGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACt                                                                                                                                         <  2:2909367/150‑1 (MQ=255)

           gTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGt                                                                                                                                       <  2:3594267/150‑1 (MQ=255)

            tAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCATTCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGtt                                                                                                                                      <  1:3961899/150‑1 (MQ=255)

            tAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGtt                                                                                                                                      <  2:2071975/150‑1 (MQ=255)

            tAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGtt                                                                                                                                      >  1:2021869/1‑150 (MQ=255)

             aGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTc                                                                                                                                     <  1:213054/150‑1 (MQ=255)

              gCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCa                                                                                                                                    >  1:2234270/1‑150 (MQ=255)

                 cTTCATCCAGTGTGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATg                                                                                                                                 <  1:190386/150‑1 (MQ=255)

                 cTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATg                                                                                                                                 <  1:2055157/150‑1 (MQ=255)

                    cATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTg                                                                                                                              >  1:2718084/1‑150 (MQ=255)

                    cATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTg                                                                                                                              <  2:80861/150‑1 (MQ=255)

                    cATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTg                                                                                                                              <  1:1955206/150‑1 (MQ=255)

                     aTCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTgg                                                                                                                             <  1:2664583/150‑1 (MQ=255)

                     aTCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTgg                                                                                                                             >  2:3697751/1‑150 (MQ=255)

                            gCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACctgc                                                                                                                      <  1:2976913/150‑1 (MQ=255)

                                 aCCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGgcg                                                                                                                 >  2:135427/1‑150 (MQ=255)

                                  ccGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGgcgc                                                                                                                <  1:1109453/150‑1 (MQ=255)

                                     cAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGt                                                                                                             <  1:3019095/150‑1 (MQ=255)

                                     cAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGt                                                                                                             <  2:2523900/150‑1 (MQ=255)

                                            cGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCa                                                                                                      <  1:4103259/150‑1 (MQ=255)

                                             gACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAg                                                                                                     <  1:2803049/150‑1 (MQ=255)

                                              aCCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGt                                                                                                    >  1:3567189/1‑150 (MQ=255)

                                                   aTTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCa                                                                                               >  1:2466391/1‑150 (MQ=255)

                                                      acgcacgcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGac                                                                                            <  1:61505/150‑1 (MQ=255)

                                                       cgcacgcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGaca                                                                                           <  2:3471511/150‑1 (MQ=255)

                                                            gcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGtt                                                                                      <  2:1838218/150‑1 (MQ=255)

                                                             ccTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGttt                                                                                     <  1:1041604/150‑1 (MQ=255)

                                                             ccTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGttt                                                                                     >  1:3434775/1‑150 (MQ=255)

                                                             ccTTCAATCATGGTTAAGGAGGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGttt                                                                                     <  2:652900/150‑1 (MQ=255)

                                                              cTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTg                                                                                    <  2:1255919/150‑1 (MQ=255)

                                                               ttCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGt                                                                                   >  1:238297/1‑150 (MQ=255)

                                                                tCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTCTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTc                                                                                  >  1:4165469/1‑150 (MQ=255)

                                                                  aaTCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGc                                                                                >  1:4210933/1‑150 (MQ=255)

                                                                   aTCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGcc                                                                               >  2:1619433/1‑150 (MQ=255)

                                                                     cATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCtt                                                                             >  1:3169801/1‑150 (MQ=255)

                                                                     cATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCtt                                                                             <  2:241439/150‑1 (MQ=255)

                                                                     cATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCtt                                                                             <  1:1209954/150‑1 (MQ=255)

                                                                      aTGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCttt                                                                            >  2:703278/1‑150 (MQ=255)

                                                                        ggTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCa                                                                          <  1:4022741/150‑1 (MQ=255)

                                                                        ggTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCa                                                                          <  1:172944/150‑1 (MQ=255)

                                                                           tAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGAc                                                                       <  2:2164667/150‑1 (MQ=255)

                                                                             aGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGt                                                                     <  2:367231/150‑1 (MQ=255)

                                                                               gATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGttt                                                                   >  2:510220/1‑150 (MQ=255)

                                                                               gATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGttt                                                                   >  1:2562302/1‑150 (MQ=255)

                                                                                aTGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTa                                                                  >  2:2700290/1‑150 (MQ=255)

                                                                                 tGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAg                                                                 <  2:1738490/150‑1 (MQ=255)

                                                                                   aaCCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCg                                                               >  2:2253565/1‑150 (MQ=255)

                                                                                     ccGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGtt                                                             <  2:3500994/150‑1 (MQ=255)

                                                                                      cGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTg                                                            >  2:3164637/1‑150 (MQ=255)

                                                                                      cGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTg                                                            <  2:1171703/150‑1 (MQ=255)

                                                                                       gCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGc                                                           <  2:112653/150‑1 (MQ=255)

                                                                                       gCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGc                                                           <  2:3059224/150‑1 (MQ=255)

                                                                                         ttAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGa                                                         >  2:1474320/1‑150 (MQ=255)

                                                                                          tAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTTCCCGAAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGaa                                                        <  2:1910982/150‑1 (MQ=255)

                                                                                          tAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGaa                                                        <  1:3901074/150‑1 (MQ=255)

                                                                                           aaCGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAAt                                                       <  1:767617/150‑1 (MQ=255)

                                                                                           aaCGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAAt                                                       <  2:390994/150‑1 (MQ=255)

                                                                                              gTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGtt                                                    <  2:2364006/150‑1 (MQ=255)

                                                                                              gTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGtt                                                    <  2:613961/150‑1 (MQ=255)

                                                                                              gTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTAGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGtt                                                    >  2:2113162/1‑150 (MQ=255)

                                                                                                aCCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTgg                                                  >  1:3336531/1‑150 (MQ=255)

                                                                                                   gTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGt                                               >  2:455293/1‑150 (MQ=255)

                                                                                                     tctcATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTaa                                             <  2:1215933/150‑1 (MQ=255)

                                                                                                     tctcATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTaa                                             >  1:1612767/1‑150 (MQ=255)

                                                                                                     tctcATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTaa                                             >  2:2615603/1‑150 (MQ=255)

                                                                                                      ctcATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAAt                                            >  1:3769714/1‑150 (MQ=255)

                                                                                                         aTCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCaa                                         <  2:899984/150‑1 (MQ=255)

                                                                                                         aTCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCaa                                         <  1:395337/150‑1 (MQ=255)

                                                                                                           ccACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACa                                       >  2:3128789/1‑150 (MQ=255)

                                                                                                            cacacaAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACAt                                      >  1:1516563/1‑150 (MQ=255)

                                                                                                                caAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAg                                  <  1:885278/150‑1 (MQ=255)

                                                                                                                caAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAg                                  <  2:4237678/150‑1 (MQ=255)

                                                                                                                  aaGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGc                                >  2:2825539/1‑150 (MQ=255)

                                                                                                                  aaGTCCGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGc                                >  1:2896871/1‑150 (MQ=255)

                                                                                                                       cGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCa                           <  1:2422959/150‑1 (MQ=255)

                                                                                                                       cGTTACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCa                           <  1:505392/150‑1 (MQ=255)

                                                                                                                          tACGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCa                        >  1:3277677/1‑150 (MQ=255)

                                                                                                                           aCGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCaa                       >  2:3841929/1‑150 (MQ=255)

                                                                                                                            cGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCaat                      >  2:1053524/1‑150 (MQ=255)

                                                                                                                            cGGTAGTATATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCaat                      <  2:3294294/150‑1 (MQ=255)

                                                                                                                              gtagtaTATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCaataa                    >  2:1545270/1‑150 (MQ=255)

                                                                                                                              gtagtaTATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCaataa                    <  1:673301/150‑1 (MQ=255)

                                                                                                                                agtaTATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAataata                  <  1:988954/150‑1 (MQ=255)

                                                                                                                                 gtaTATTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATAc                 >  1:3123059/1‑150 (MQ=255)

                                                                                                                                  tataTTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATAcc                >  1:2636662/1‑150 (MQ=255)

                                                                                                                                   atatTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCg               <  1:3657562/150‑1 (MQ=255)

                                                                                                                                   atatTGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCg               <  2:4018214/150‑1 (MQ=255)

                                                                                                                                      ttGTTTTACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCaa            >  2:3976326/1‑150 (MQ=255)

                                                                                                                                         ttttACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATaa         >  1:707142/1‑150 (MQ=255)

                                                                                                                                          tttACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATaaa        >  2:2928858/1‑150 (MQ=255)

                                                                                                                                           ttACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAAt       <  2:2036864/150‑1 (MQ=255)

                                                                                                                                           ttACCCGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAAt       >  1:1679974/1‑150 (MQ=255)

                                                                                                                                               ccGCAAATAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCa   <  2:4177528/150‑1 (MQ=255)

CGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAA‑AATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAG  >  AP012306/574075‑574367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`