Predicted mutation
evidence seq id position mutation annotation gene description
RA AP012306 3,829,379 G→A intergenic (+75/‑11) miaA → / → hfq delta(2)‑isopentenylpyrophosphate tRNA‑adenosine transferase/HF‑I, host factor for RNA phage Q beta replication

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,829,3790GA100.0% 1350.4 / NA 334intergenic (+75/‑11)miaA/hfqdelta(2)‑isopentenylpyrophosphate tRNA‑adenosine transferase/HF‑I, host factor for RNA phage Q beta replication
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (179/155);  total (179/155)

Only 100 of 342 total aligned reads displayed.

CTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGT  >  AP012306/3829232‑3829525
                                                                                                                                                   |                                                                                                                                                  
cTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGaaa                                                                                                                                                  <  2:1638496/150‑1 (MQ=255)
 tGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGaaaa                                                                                                                                                 <  1:1438589/150‑1 (MQ=255)
    cTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAaga                                                                                                                                              <  1:183263/150‑1 (MQ=255)
     ttGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAagag                                                                                                                                             <  2:3829844/150‑1 (MQ=255)
      tGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGGTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGCGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGAACAAATAAGCATATAAGAAAAagaga                                                                                                                                            >  2:2451488/1‑150 (MQ=255)
        aCAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAgagaga                                                                                                                                          <  1:3825368/150‑1 (MQ=255)
        aCAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAgagaga                                                                                                                                          <  2:2783092/150‑1 (MQ=255)
          aGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAAt                                                                                                                                        <  1:3165570/150‑1 (MQ=255)
           gTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATg                                                                                                                                       <  1:3986741/150‑1 (MQ=255)
           gTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATg                                                                                                                                       <  1:2216476/150‑1 (MQ=255)
                aaaCCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTaa                                                                                                                                  <  1:932926/150‑1 (MQ=255)
                 aaCCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAg                                                                                                                                 <  2:3650457/150‑1 (MQ=255)
                  aCCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAgg                                                                                                                                <  1:3021695/150‑1 (MQ=255)
                    cAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAgggg                                                                                                                              >  2:3880773/1‑150 (MQ=255)
                          aGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAGTAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCt                                                                                                                        >  1:1651703/1‑150 (MQ=255)
                           ggCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCtt                                                                                                                       >  1:4216970/1‑150 (MQ=255)
                            gcgcGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCttt                                                                                                                      <  1:401282/150‑1 (MQ=255)
                             cgcgTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTa                                                                                                                     <  1:3316886/150‑1 (MQ=255)
                               cgTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACa                                                                                                                   >  1:1040668/1‑150 (MQ=255)
                                 tGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAg                                                                                                                 >  2:497664/1‑150 (MQ=255)
                                  gACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGa                                                                                                                <  1:549718/150‑1 (MQ=255)
                                  gACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGa                                                                                                                <  2:121852/150‑1 (MQ=255)
                                     gAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGTATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATcc                                                                                                             <  1:1147538/150‑1 (MQ=255)
                                     gAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATcc                                                                                                             >  1:3719681/1‑150 (MQ=255)
                                       aGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGt                                                                                                           >  2:2013638/1‑150 (MQ=255)
                                          aTTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTcc                                                                                                        >  1:4162547/1‑150 (MQ=255)
                                                 gttgttGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGc                                                                                                 >  2:4040240/1‑150 (MQ=255)
                                                  ttgttgGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCa                                                                                                >  2:3091579/1‑150 (MQ=255)
                                                  ttgttgGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCa                                                                                                <  1:1562827/150‑1 (MQ=255)
                                                   tgttgGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCAc                                                                                               <  1:2489969/150‑1 (MQ=255)
                                                    gttgGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACt                                                                                              >  2:3776964/1‑150 (MQ=255)
                                                       ggTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCg                                                                                           >  2:2571910/1‑150 (MQ=255)
                                                               cGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCGATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAAc                                                                                   <  2:1031127/150‑1 (MQ=255)
                                                                gCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACg                                                                                  >  2:366628/1‑150 (MQ=255)
                                                                 cAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACgt                                                                                 >  2:1323655/1‑150 (MQ=255)
                                                                  aGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACgtg                                                                                >  1:829880/1‑150 (MQ=255)
                                                                  aGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACgtg                                                                                >  2:1838525/1‑150 (MQ=255)
                                                                      tGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTAAAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTcc                                                                            <  2:3911325/150‑1 (MQ=255)
                                                                       gAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCa                                                                           <  1:699325/150‑1 (MQ=255)
                                                                            tgtACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTc                                                                      <  1:863894/150‑1 (MQ=255)
                                                                              tACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCta                                                                    <  2:2692181/150‑1 (MQ=255)
                                                                                 aaTTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCtattt                                                                 >  1:2335126/1‑150 (MQ=255)
                                                                                 aaTTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCtattt                                                                 <  1:2781317/150‑1 (MQ=255)
                                                                                  aTTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCtattta                                                                >  1:3094937/1‑150 (MQ=255)
                                                                                   ttGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCtatttat                                                               <  1:1290081/150‑1 (MQ=255)
                                                                                     gagaCGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTatttattt                                                             >  1:2043288/1‑150 (MQ=255)
                                                                                        aCGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGt                                                          <  1:2993590/150‑1 (MQ=255)
                                                                                        aCGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGt                                                          <  2:1491338/150‑1 (MQ=255)
                                                                                             tCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATg                                                     <  2:1592187/150‑1 (MQ=255)
                                                                                              cGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATgg                                                    >  2:1715566/1‑150 (MQ=255)
                                                                                              cGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATgg                                                    <  2:116813/150‑1 (MQ=255)
                                                                                              cGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATgg                                                    <  1:3258123/150‑1 (MQ=255)
                                                                                               gTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGt                                                   >  1:75775/1‑150 (MQ=255)
                                                                                                tGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTa                                                  <  1:3917707/150‑1 (MQ=255)
                                                                                                 gcgcAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTAt                                                 >  1:4111706/1‑150 (MQ=255)
                                                                                                 gcgcAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTAt                                                 <  1:443377/150‑1 (MQ=255)
                                                                                                   gcAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTa                                               >  1:1885701/1‑150 (MQ=255)
                                                                                                   gcAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTa                                               >  1:3470532/1‑150 (MQ=255)
                                                                                                   gcAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTa                                               <  1:2672838/150‑1 (MQ=255)
                                                                                                    cAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTaa                                              <  1:1736351/150‑1 (MQ=255)
                                                                                                    cAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTaa                                              <  1:2015985/150‑1 (MQ=255)
                                                                                                    cAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTaa                                              >  1:3649041/1‑150 (MQ=255)
                                                                                                        tttttCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTg                                          <  2:3239651/150‑1 (MQ=255)
                                                                                                            tCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAg                                      >  1:673362/1‑150 (MQ=255)
                                                                                                             cAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAgg                                     <  2:2614854/150‑1 (MQ=255)
                                                                                                             cAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAgg                                     <  1:3142634/150‑1 (MQ=255)
                                                                                                              aGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAggg                                    >  2:2855026/1‑150 (MQ=255)
                                                                                                               gAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGc                                   <  2:2202935/150‑1 (MQ=255)
                                                                                                                aaTCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCa                                  >  2:2984197/1‑150 (MQ=255)
                                                                                                                  tCGAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCaaa                                >  1:2939586/1‑150 (MQ=255)
                                                                                                                    gAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATc                              <  2:923871/150‑1 (MQ=255)
                                                                                                                    gAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATc                              <  1:467665/150‑1 (MQ=255)
                                                                                                                    gAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATc                              >  1:3749286/1‑150 (MQ=255)
                                                                                                                    gAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATc                              >  1:2407884/1‑150 (MQ=255)
                                                                                                                    gAAAGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATc                              <  2:3442452/150‑1 (MQ=255)
                                                                                                                      aaGGTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGa                            >  1:1174058/1‑150 (MQ=255)
                                                                                                                        ggTTCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGt                          >  2:3143504/1‑150 (MQ=255)
                                                                                                                           tCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGGGTCtt                       >  2:737536/1‑150 (MQ=255)
                                                                                                                           tCAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCtt                       <  1:212525/150‑1 (MQ=255)
                                                                                                                            cAAAGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCttt                      >  2:1128329/1‑150 (MQ=255)
                                                                                                                             aaaGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCtttt                     >  1:626583/1‑150 (MQ=255)
                                                                                                                              aaGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTg                    >  2:3992961/1‑150 (MQ=255)
                                                                                                                               aGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGa                   >  2:879545/1‑150 (MQ=255)
                                                                                                                               aGTACAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGa                   >  2:596847/1‑150 (MQ=255)
                                                                                                                                  aCAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCa                >  1:2910621/1‑150 (MQ=255)
                                                                                                                                   cAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAg               <  1:149755/150‑1 (MQ=255)
                                                                                                                                   cAAATAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAg               >  2:4113088/1‑150 (MQ=255)
                                                                                                                                     aaTAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGtt             >  2:3539674/1‑150 (MQ=255)
                                                                                                                                     aaTAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGtt             <  1:271134/150‑1 (MQ=255)
                                                                                                                                      aTAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTc            <  1:3385910/150‑1 (MQ=255)
                                                                                                                                       tAAGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCg           <  1:2810046/150‑1 (MQ=255)
                                                                                                                                        aaGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGt          >  1:179697/1‑150 (MQ=255)
                                                                                                                                        aaGCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGt          >  2:460527/1‑150 (MQ=255)
                                                                                                                                          gCATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGa        <  1:953609/150‑1 (MQ=255)
                                                                                                                                           cATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAt       >  2:1420446/1‑150 (MQ=255)
                                                                                                                                           cATATAAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGAt       <  1:3578400/150‑1 (MQ=255)
                                                                                                                                             tataAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATcc     >  2:2082487/1‑150 (MQ=255)
                                                                                                                                              ataAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCt    >  1:2586459/1‑150 (MQ=255)
                                                                                                                                              ataAGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCt    >  2:3290021/1‑150 (MQ=255)
                                                                                                                                                aaGAAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCCTTTGATCAGTTCGTGATCCTGt  <  2:3411678/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTGGCTTGACAGTGAAAAACCAGAACAGGCGCGTGACGAAGTATTACAGGTTGTTGGTGCTATCGCAGGCTGAATGTGTACAATTGAGACGTATCGTGCGCAATTTTTTCAGAATCGAAAGGTTCAAAGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGT  >  AP012306/3829232‑3829525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑