Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306257,1370TG23.9% 442.2 / 11.0 201L496V (TTG→GTG) betTcholine transporter of high affinity
Reads supporting (aligned to +/- strand):  ref base T (116/37);  new base G (48/0);  total (164/37)
Fisher's exact test for biased strand distribution p-value = 2.10e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 270 total aligned reads displayed.

CCCCGGCTGGCTGCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTACACTAAACAG  >  AP012306/256989‑257286
                                                                                                                                                    |                                                                                                                                                     
ccccGGCTGGCTGCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGtt                                                                                                                                                      <  1:1891598/150‑1 (MQ=255)
 cccGGCTGGCTGCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTg                                                                                                                                                     <  1:4033861/150‑1 (MQ=255)
 cccGGCTGGCTGCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTg                                                                                                                                                     <  2:1304727/150‑1 (MQ=255)
   cGGCTGGCTGCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGta                                                                                                                                                   <  2:286178/150‑1 (MQ=255)
     gctggctgCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGtata                                                                                                                                                 <  2:1528478/150‑1 (MQ=255)
      ctggctgCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATaa                                                                                                                                                >  1:1385315/1‑150 (MQ=255)
       tggctgCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATaaa                                                                                                                                               <  2:899484/150‑1 (MQ=255)
       tggctgCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATcaa                                                                                                                                               >  2:512462/1‑150 (MQ=255)
        ggctgCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAAt                                                                                                                                              >  1:2603120/1‑150 (MQ=255)
        ggctgCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAAt                                                                                                                                              <  1:2245609/150‑1 (MQ=255)
         gctgCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAAtc                                                                                                                                             <  1:3197988/150‑1 (MQ=255)
              gcgTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGa                                                                                                                                        >  1:3238627/1‑150 (MQ=255)
              gcgTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGa                                                                                                                                        >  1:2805178/1‑150 (MQ=255)
                 tcttctGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAgg                                                                                                                                     >  1:1668223/1‑150 (MQ=255)
                 tcttctGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAgg                                                                                                                                     <  2:108633/150‑1 (MQ=255)
                  cttctGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGt                                                                                                                                    <  2:480006/150‑1 (MQ=255)
                   ttctGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTa                                                                                                                                   >  1:590558/1‑150 (MQ=255)
                   ttctGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTa                                                                                                                                   <  2:2656344/150‑1 (MQ=255)
                    tctGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTag                                                                                                                                  <  2:3060150/150‑1 (MQ=255)
                      tGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTagaa                                                                                                                                <  1:3761010/150‑1 (MQ=255)
                        gTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGGagaaga                                                                                                                              >  1:1078466/1‑150 (MQ=255)
                         tCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGAt                                                                                                                             >  1:1493348/1‑150 (MQ=255)
                           ggTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTa                                                                                                                           <  1:2386172/150‑1 (MQ=255)
                            gTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTAc                                                                                                                          <  1:1112894/150‑1 (MQ=255)
                            gTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTAc                                                                                                                          <  1:3720115/150‑1 (MQ=255)
                              ggCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGTAGAAGATTAccg                                                                                                                        >  1:3626758/1‑150 (MQ=255)
                               gCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTTAAGGTAGAAGATTAccgc                                                                                                                       >  2:1022782/1‑150 (MQ=255)
                                cGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTAccgcc                                                                                                                      <  2:3682675/150‑1 (MQ=255)
                                cGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTAccgcc                                                                                                                      <  1:3488339/150‑1 (MQ=255)
                                cGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGGAGAAGATTAccgcc                                                                                                                      >  2:2776912/1‑150 (MQ=255)
                                cGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAAGTAGAAGATTAccgcc                                                                                                                      >  1:1647275/1‑150 (MQ=255)
                                 gATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATTAAATTCTTAAGGCAGAAGATTAgcgccg                                                                                                                     >  2:3889229/1‑150 (MQ=255)
                                 gATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTAccgccg                                                                                                                     >  1:1361246/1‑150 (MQ=255)
                                 gATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTAccgccg                                                                                                                     >  1:1001421/1‑150 (MQ=255)
                                      gCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGaaa                                                                                                                >  1:2970828/1‑150 (MQ=255)
                                      gCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATTAATCTCTGAAAGTAGAAGATTAacgccggggaa                                                                                                                >  2:943882/1‑145 (MQ=255)
                                              aCGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGGGAAAGGGCCAAc                                                                                                        >  2:696569/1‑150 (MQ=255)
                                                gCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACcg                                                                                                      >  2:2188433/1‑150 (MQ=255)
                                                        tGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATaccg                                                                                              >  1:210839/1‑150 (MQ=255)
                                                         gCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGGGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCTGTGAAAGATCCAACCGCCATacccc                                                                                             >  2:349913/1‑148 (MQ=255)
                                                               gaCTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGGAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACcgcg                                                                                       >  1:3882111/1‑150 (MQ=255)
                                                                 cTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGAc                                                                                     >  1:985017/1‑150 (MQ=255)
                                                                 cTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGGGAAAGGGGCAACCGCGATACCGCACCGCGAc                                                                                     >  2:795937/1‑150 (MQ=255)
                                                                   aaCGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCg                                                                                   >  1:1759076/1‑150 (MQ=255)
                                                                      gggATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGGGCCAACCGCGATACCGCACCGCGACCGCTg                                                                                >  1:1590120/1‑150 (MQ=255)
                                                                       ggATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGGAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTgg                                                                               >  1:2630691/1‑150 (MQ=255)
                                                                         atatCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGATGTATAAATCTCTGAACGTAAAAGATTCACGACGTGAAAGTGCCGACCCCGGTACCGCACCGCAACCGCTgggg                                                                             >  2:3772025/1‑150 (MQ=255)
                                                                          tatCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGc                                                                            >  2:2559823/1‑150 (MQ=255)
                                                                           atCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGGAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCt                                                                           >  1:1709032/1‑150 (MQ=255)
                                                                              cgcgCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCa                                                                        >  1:1030160/1‑150 (MQ=255)
                                                                                 gcTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGGAGAAGATTACCGCCGGGAAAGTGGCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCCGGa                                                                     >  2:1973867/1‑150 (MQ=255)
                                                                                 gcTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGGAGAAGATTACCCCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGa                                                                     >  2:3966463/1‑150 (MQ=255)
                                                                                   tGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATc                                                                   >  2:135705/1‑150 (MQ=255)
                                                                                    gCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCg                                                                  >  1:3914618/1‑150 (MQ=255)
                                                                                        aaCACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGCAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATTCCGCACCCCCACCGCTGGGGCTTCAGGGTCGGCTg                                                              >  2:2126009/1‑150 (MQ=255)
                                                                                          ccccggggTGATTAGGGGGCTGCCGTTCTGTTTTGTGTTCTTCTTGGTGAGGGGGGGTTTGTATAAATCTCTGAGGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAg                                                            <  2:1405892/144‑1 (MQ=255)
                                                                                           accacGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGc                                                           >  1:2352614/1‑150 (MQ=255)
                                                                                           accacGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGGACCGCGACCGCTGGGGCTTTAGGGTCGCCTGAGc                                                           >  2:739449/1‑150 (MQ=255)
                                                                                             cacGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTg                                                         >  1:3332887/1‑150 (MQ=255)
                                                                                              acGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGGAGAAGATTACCGCCCTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTgg                                                        >  1:2179806/1‑150 (MQ=255)
                                                                                              acGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTgg                                                        >  2:1725562/1‑150 (MQ=255)
                                                                                               cGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGa                                                       >  2:1225722/1‑150 (MQ=255)
                                                                                                   gATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGaaaaa                                                   >  1:1288749/1‑150 (MQ=255)
                                                                                                    aTTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGaaaaaa                                                  >  1:3377910/1‑150 (MQ=255)
                                                                                                    aTTATGGGGCTGCCGTTCAGCTATGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGGAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCGGGaaaaaa                                                  >  2:176215/1‑150 (MQ=255)
                                                                                                     ttATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGGAGAAGATTACCGCCGGGGAAGGGGCAACCGCGGTACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAAc                                                 >  2:2906066/1‑150 (MQ=255)
                                                                                                        tGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGTAGAAGATTACCCCCGTGAAAGTGCCAACCCCGATACCGCACCGCGACCGCTGGGGCTTCAGGATTGCCTGAGCTGGAAAAAACGtc                                              >  2:560411/1‑150 (MQ=255)
                                                                                                         ggggCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGtct                                             >  1:1211587/1‑150 (MQ=255)
                                                                                                           ggCTGCCGTTCAGTTTTGTGTTTTTTTGGGGGGGGGGGGGTTTTTATAAATCTGGGAGGGTAGAAGTTTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGtctct                                           <  2:1415645/150‑1 (MQ=255)
                                                                                                              tGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTcgc                                        >  1:510469/1‑150 (MQ=255)
                                                                                                               gCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGGAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTcgcg                                       >  1:41462/1‑150 (MQ=255)
                                                                                                                 cGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGAACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGcc                                     >  2:1603479/1‑150 (MQ=255)
                                                                                                                 cGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGGAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGcc                                     >  2:3146383/1‑150 (MQ=255)
                                                                                                                  gTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGGAGAAGATTACCGCCGTGGAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGGTCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCt                                    >  2:1840939/1‑150 (MQ=255)
                                                                                                                     cAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTACCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCtgat                                 >  1:842844/1‑150 (MQ=255)
                                                                                                                      aGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCtgatg                                >  1:2776504/1‑150 (MQ=255)
                                                                                                                        cTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGaa                              >  1:91391/1‑150 (MQ=255)
                                                                                                                          ttGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTTAGCTGGAAAAAACGGCTCTCCCGGCTGATGGAtt                            >  2:2570048/1‑150 (MQ=255)
                                                                                                                          ttGTGATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGCAGAAGATTACCCCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAAtt                            >  1:719068/1‑150 (MQ=255)
                                                                                                                           tgtgTTCTTCTTGGTGAGGGGGGGGTTTAATAATTCTCGGAAGGTAGAAGTTTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTa                           <  2:3308459/150‑1 (MQ=255)
                                                                                                                            tgatttttcttgGTGAGGGGGGGGTTTTAAAAATTTCGGAGGGAAGAAGTTTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTAt                          <  1:2662697/144‑1 (MQ=255)
                                                                                                                             tgATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATc                         >  2:1118140/1‑150 (MQ=255)
                                                                                                                             tgATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGGAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATc                         >  2:834746/1‑150 (MQ=255)
                                                                                                                             tgATCTTCTTCGTGATGGCGGGGGTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATc                         >  1:201510/1‑150 (MQ=255)
                                                                                                                               aTCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCg                       >  1:3925766/1‑150 (MQ=255)
                                                                                                                                    cttcGTGATGGCGGGGTTGTATAAATTTCTTCAGGCAGAAGATTACCGCCCGGAAAGGGCCAACCGCGATACCGCCCCACGACCGCCCGGGCGTTCGGACCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCCGGCAc                  >  2:1480463/1‑150 (MQ=255)
                                                                                                                                    cttcGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCAc                  >  2:1044949/1‑150 (MQ=255)
                                                                                                                                     ttcGTGATGGCGGGGGTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGGGAAAGTGCCAAACGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCCGAGCTGGGAAAAACGTCTCTCGCGCCTGATTAATTATCCGGGCAcg                 >  2:2051694/1‑150 (MQ=255)
                                                                                                                                       cGTGATGGCGGGGTTGTATAAATCTCTGAAGGGAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCAcgcg               >  2:3417449/1‑150 (MQ=255)
                                                                                                                                         tGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGtt             >  1:3421194/1‑150 (MQ=255)
                                                                                                                                         tGATGGCGGGGGTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAATGCCAACCGCGATACCGCACCGCGACCGCTGGGGGTTCAGGATCGCCCGAGCTGGGAAAAACGTCTCTCGCGCCTTATGGATTATCCGGGCCcgcggt             >  2:418777/1‑148 (MQ=255)
                                                                                                                                            tGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTaca          >  2:3225291/1‑150 (MQ=255)
                                                                                                                                            tGGCGGGGTTGTATAAATCTCTGAAGGAAGAAGACTACCCCCGTGTAAGGGGCAAACCCGATACCGGACCGCGACCGCTTGGGGTTCAGGATCGCGTGAGCTTGGAAAAACGTCTCTTTCGCCTTGTGAAATATCCGGGCACGCGTTact          >  2:663752/1‑149 (MQ=255)
                                                                                                                                             gggggggTTGTATAATTCTGGGAGGGTAGATGTTTCCCGCCGTGAAAGTGCCAACCTCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTacac         <  2:2230736/147‑1 (MQ=255)
                                                                                                                                              gCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTACACt        >  1:1536550/1‑150 (MQ=255)
                                                                                                                                              gCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTACACt        >  1:287070/1‑150 (MQ=255)
                                                                                                                                                ggggTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTACACTaa      >  2:1945247/1‑150 (MQ=255)
                                                                                                                                                ggggTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTACACTaa      >  1:3999177/1‑150 (MQ=255)
                                                                                                                                                 gggTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTACACTaaa     >  1:309884/1‑150 (MQ=255)
                                                                                                                                                    ttGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTACACTAAACAg  >  2:2416854/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CCCCGGCTGGCTGCGCGTCTTCTGGTCGGTGGCGATTGGCCTGCTGACGCTCGGCATGCTGATGACTAACGGGATATCCGCGCTGCAAAACACCACGGTGATTATGGGGCTGCCGTTCAGCTTTGTGATCTTCTTCGTGATGGCGGGGTTGTATAAATCTCTGAAGGTAGAAGATTACCGCCGTGAAAGTGCCAACCGCGATACCGCACCGCGACCGCTGGGGCTTCAGGATCGCCTGAGCTGGAAAAAACGTCTCTCGCGCCTGATGAATTATCCGGGCACGCGTTACACTAAACAG  >  AP012306/256989‑257286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑