Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,182,8610AC22.7% 277.7 / 14.9 110L198F (TTA→TTC) yiaNpredicted transporter
Reads supporting (aligned to +/- strand):  ref base A (66/19);  new base C (0/25);  total (66/44)
Fisher's exact test for biased strand distribution p-value = 3.88e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 188 total aligned reads displayed.

ATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCA‑TTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCT‑CCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGC  >  AP012306/3182712‑3183010
                                                                                                                                                       |                                                                                                                                                       
  tattCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCG‑TTT‑CCCCCGGCCTGATGATGGGGGCAACGCCGATGCTTTCCTGGGGGTGGGCGGGCCGGCGCCTTTATTTT‑cc                                                                                                                                                      >  2:2753179/1‑150 (MQ=255)
  tattCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCA‑TTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑cc                                                                                                                                                      <  2:1112579/150‑1 (MQ=255)
     tCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCC‑TTT‑CCCCCTGCATGTTGATGGGGGCAAAGCTGATGTTTTCCTGGGGGGGGGAGGCCCGGCGCCTTTATTTA‑ccccc                                                                                                                                                   >  2:1419840/1‑149 (MQ=255)
     tCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCA‑TTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCg                                                                                                                                                   <  1:3518027/150‑1 (MQ=255)
       ccccTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCA‑TTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGcc                                                                                                                                                 <  1:3451050/150‑1 (MQ=255)
         ccTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCACTTG‑CCCCT‑GCGTGCTGATGGGCGCAACACCGATGCTTAACTTGGGGTGGGGGGGCAGATGCCCTCATTTT‑TCCCGcccc                                                                                                                                               >  2:3062191/1‑148 (MQ=255)
         ccTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCA‑TTG‑CCCCTGGCATGATGATGGGGGCAACGCTGGTGGTTTCCTGGTGGGGGGGGGGCCGGCGCCCTTATTTT‑Ccccccccg                                                                                                                                               >  2:1167617/1‑148 (MQ=255)
         ccTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCA‑TTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAg                                                                                                                                               <  1:421475/150‑1 (MQ=255)
         ccTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCA‑TTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAg                                                                                                                                               <  1:3025815/150‑1 (MQ=255)
              cATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCA‑TTG‑CCCCTGGCAGGATGATGGGCGCAACGTGGATGCTTACCTGGTGGTGGAAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCaaaa                                                                                                                                          <  2:3677483/150‑1 (MQ=255)
                 tCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCG‑TTT‑CCCCCCGCATGATGATTGGCGCAAAGCTGATGCTTTCCTGGGGGGGGGGGGGCAGGCGCCCTTATTTT‑TCCCCCCCCCaaaaaac                                                                                                                                       >  2:1591625/1‑148 (MQ=255)
                 tCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCG‑TTGTTCCCCGGCA‑GATGACCGGCGCCACGCCCAGACTTGCCTTGTGGGGGCTGGCCAACTGCCCTCATTTACCCCCCCCCGCaaaaac                                                                                                                                        >  2:2847676/1‑149 (MQ=255)
                  ctgTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCA‑TTG‑CCCCTGGCATGATGAGGGGCGCACCGCTGATGCTTACCGGGGGGGGGCAGGCCAGTCCCTTTAATTTA‑CCCCGCCAGCAAAAAGCa                                                                                                                                      <  2:2820852/148‑1 (MQ=255)
                      ttattaTCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGGA‑TTT‑GCCCCGGCATGATGATGGGGGCAACGCTGGTTCTTGCCTGGGGGTGGGAGGCCGGACGCCTTAATTTA‑TCCCCCCAGCAAAAAAAAACGa                                                                                                                                  >  2:3427545/1‑150 (MQ=255)
                      ttattaTCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGGA‑TTT‑CCCCTGGCGTGGTGATTGGGGCCACGCTGATGCTTTCCTTGTGGGGGTAGGCCCGGCGGCTTTATTTT‑TCCCCCCCGCAAAAaacaacaa                                                                                                                                  >  2:201846/1‑148 (MQ=255)
                        attatCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCG‑ATT‑CCCCCGGCATTATGATGGGGGCGACGCTGATGTTTTCCTGGTGGGGGGAGGCCAGACGCCTTAATTTA‑TCCCGCCCGCAAAAAGCAACGATg                                                                                                                                >  2:3466085/1‑150 (MQ=255)
                           atCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCC‑TTG‑CCCCCCGCCTGATGATGGGGGCAACGCTGATGCTTTCCTGGTGGGGGGAGGCCCGTCGCCTTTATTTT‑CCCCCCCCCCAAAAAACAAAAATGCAg                                                                                                                             >  2:2716187/1‑150 (MQ=255)
                                cGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGGA‑TTT‑CCCCCGGCGTGATGATGGGCGCAACCCTTATGCTTACCTGGGGGTGGGGGGCCCGTCGCCTTTATTTA‑CCCCGCCCCCAAAAAACAACGATGGAGGaaaa                                                                                                                        >  2:2415045/1‑149 (MQ=255)
                                   cGTTTCCCGCGGATTATCCAGCAGAAGGCTGTTAAGGGAGGGCA‑TTG‑CCCTGGGCATGATGTTGGGCGAAACGGTGAGGCTTACCTGGGGGGGGAAGCCCAGTCGCCTTAATTAC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTg                                                                                                                     <  2:4011343/150‑1 (MQ=255)
                                   cGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCC‑TTT‑CCCCCCGCCTGTTGATTGGGGCCACGCCGGTGCTTTCCTGGGGGTGGCGGGCCCGTTGCCCTTATTTA‑CCCCCCCCCCAAAAAACAACCATGCAGGGAAACTg                                                                                                                     >  2:2249799/1‑150 (MQ=255)
                                       tCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGGC‑TTT‑CCCCCCGCCTGGTGGTGGGGGCAACGCCGATGGTTAACCGGTGGGGGCGGGGCCATCGCCTTTATTTA‑CCCCCCCCGCAAAAAACAAACGTGCCGGAAAACTTGccc                                                                                                                 >  2:3224604/1‑148 (MQ=255)
                                        ccAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCA‑TTT‑CCCCCGGCATGATGATGGGGGCAACGCTGATGCTTACCCGGTGGGGGGAGGGCAGTCGCCTTTATTTT‑ACCCCCCCGCAAAAAAAAAAGAAGCAGGAAAACTTGcccc                                                                                                                >  2:3817836/1‑147 (MQ=255)
                                           gCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCA‑TTG‑CCCCCGGCCTGATGATTGGGGCAACGCTGATGCTTTCCTGGTGGTGGGAGGCCAGTCGCCTTAATTTA‑TCCCCCCAGCAAAAAGCAAAGATGCAGGAAATCTgggcccctt                                                                                                             >  2:1697365/1‑143 (MQ=255)
                                            cGGATTATCCATCAGTAAGCTGTTTATGGCGGGGA‑TTG‑CCCCCCGGGTGATGATGGGGGCAAAGCTGATGTTTTCCTTGTGGGGGGAGGGCAGGCGCCTTAATTTT‑CCCCCCCCGCAAAAAACAAAGATGGAGGAAAACTGGCACTCttt                                                                                                            >  2:3077720/1‑150 (MQ=255)
                                            cGGATTATCCATCAGTAAGCTGTTTATGGCGGGCA‑TTT‑CCCCCGGCATGGTGATGGGGGCAACGCCGATGCTTACCTGGTGGGGGGGGGCCCGGCGCCTTCATTTT‑TCCCCCCCGCAAAAAACAACGATGGAGGAAAAATGGCACTCttt                                                                                                            >  2:1164183/1‑150 (MQ=255)
                                            cGGATTATCCATCAGTAAGCTGTTTATGGCGGGCA‑TTG‑CCCCGGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCTTTATTTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCttt                                                                                                            <  2:1505827/150‑1 (MQ=255)
                                              gATTATCCATCAGTAAGCTGTTTATGGCGGGGA‑CTG‑GCCCCGGCATGTTGGTGGGGGCGACGGCGATGCTTACCCGGGGGTGGGAGGGCGGGGGCCCTAAATTA‑ACCCCCCCGCGAAAAGCAAAGAAGCAGGAAAACTGGGACTCTTTTg                                                                                                          >  2:3359151/1‑150 (MQ=255)
                                               aTTATCCATCAGTAAGCTGTTTATGGCGGGGC‑TTG‑CCCCCCGCATGATGATTGGCGCAACGCTGATGCTTACCTGGTGGGGGAAGGGCAATCGGCCTAATTTA‑TCCCCCCCACAAAAAAAAAAGATGCAGGGAAATTTGCACTCtttttt                                                                                                         >  2:1399070/1‑148 (MQ=255)
                                                     cATCAGTAAGCTGTTTATGGCGGGCA‑TTG‑CCCCTGGCATGATGAGGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTATTTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCgg                                                                                                   <  1:375974/150‑1 (MQ=255)
                                                      aTCAGTAAGCTGTTTAGGGCGGGCA‑TTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGt                                                                                                  <  1:2513005/150‑1 (MQ=255)
                                                       tCAGTAAGCTGTTTATGGCGGGGA‑TTT‑CCCCCCGCATGATGGTGGGGGGGACGCTGATGCTTTCCTTGTGGGGGGGGGGCAGGCGGCCTAATTTT‑ACCCCCCCGCCAAAAGCAACGAAGCAGGGAAACTGGGCCTCTTTTTTCTCCCGTa                                                                                                 >  2:3328159/1‑150 (MQ=255)
                                                          gTAGGCTGTTTAGGGCGGGCG‑TCG‑CCCCTGGCAGGAGGTGGGGCCCACCGCTGAGGCTTCCGTGGGGGGGGAAGGCAAGTCGCTTTATTTCC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGGCTCCGGTATCt                                                                                              <  2:758460/150‑1 (MQ=255)
                                                          gTAAGCTGTTTATGGCGGGCA‑TTT‑CCCCCTGCCTGATTATTGGGGCAACGCTTATGCTTTCCTGGTGGGGGGAGGCCACGCGCCCTTATTTA‑CCCCCCCCGCCAAAAACAACGATGCAGGAAATCTTGCCCTCTTTTTTTTCCCGTATCt                                                                                              >  2:1093096/1‑150 (MQ=255)
                                                           tAAGCTGTTTATGGCGGGCA‑TTG‑CCCCGGGCATGATGAGGGGCGCAACGCTGATGCTTCCCTGGTGGTGGCAGGCCAGTCGCCTTATTTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTg                                                                                             <  2:3197138/150‑1 (MQ=255)
                                                              gCTGTTTATGGCGGGCA‑TTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTCCCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGgc                                                                                          <  1:1978457/150‑1 (MQ=255)
                                                                      tGGCGGGCA‑TTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGCGGTGGCAGGCCAGTCGCCTTCATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGCATCTGGGCGCTGTttc                                                                                  >  2:1961620/1‑150 (MQ=255)
                                                                       ggCGGGCA‑TTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttct                                                                                 <  1:551173/150‑1 (MQ=255)
                                                                       ggCGGGCA‑TTG‑CCCCTGGATTGATGAGGGGCGCACCGCTGATGCTTCCCTGGGGGGGGCGGGCCAGTCGCTTTAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttct                                                                                 <  2:57778/150‑1 (MQ=255)
                                                                         cGGGCA‑TTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttcttc                                                                               >  2:2512790/1‑150 (MQ=255)
                                                                         cGGGCA‑TTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTttcttc                                                                               >  2:1559582/1‑150 (MQ=255)
                                                                           ggCT‑TTG‑CCCCTGGCATGATGTTGGGCGCAACGATGGTGCTGCCGTGGTGGTGGCGGGCCATTCGCGTTATTTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCa                                                                             <  2:3934983/150‑1 (MQ=255)
                                                                             cA‑TTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGt                                                                           >  2:211245/1‑150 (MQ=255)
                                                                              a‑TTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGtc                                                                          >  2:3799993/1‑150 (MQ=255)
                                                                                       cTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCatta                                                                   >  2:1287581/1‑150 (MQ=255)
                                                                                        tGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCattat                                                                  >  2:3413438/1‑150 (MQ=255)
                                                                                        tGGCATGATGATGGCCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCTTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCattat                                                                  <  1:1027309/150‑1 (MQ=255)
                                                                                         ggCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCattatt                                                                 >  2:842398/1‑150 (MQ=255)
                                                                                         ggCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCattatt                                                                 >  2:885285/1‑150 (MQ=255)
                                                                                         ggCATGAGGAGGGCCGCACCGCTGATGCTTCCCTGGTGGGGGCGGGCCAGTCGCTTTATTTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCattatt                                                                 <  2:915482/150‑1 (MQ=255)
                                                                                          gCATGATGATGGGCGCACCGCTGTTGCTTCCCGGGGGGGGGGGGGCCAGTCGCTTTAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCCGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTg                                                                <  2:3302013/150‑1 (MQ=255)
                                                                                          gCATGATGATGGGCGCAACGCTGATGCTTCCTTGGTGGGGGCAGGCCAGTCGCTTTATTTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTg                                                                <  2:3157271/150‑1 (MQ=255)
                                                                                          gCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTg                                                                >  1:986499/1‑150 (MQ=255)
                                                                                              gatgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCgg                                                            >  1:1848662/1‑150 (MQ=255)
                                                                                               atgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCggg                                                           >  1:1031957/1‑150 (MQ=255)
                                                                                               atgatgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCggg                                                           >  1:2866926/1‑150 (MQ=255)
                                                                                                 gaggggCGAAACGCTGATGCTTACTTGGTGGTGGAGGGCAAGTCGCCTTAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGtt                                                         <  1:3466909/147‑1 (MQ=255)
                                                                                                  tggggCGCACCGTTACTGCTTCCGTGGTGGGGGCAGGCCAGTCCCTTTAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGttt                                                        <  2:547609/148‑1 (MQ=255)
                                                                                                  atgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGttt                                                        >  1:2618492/1‑150 (MQ=255)
                                                                                                   tgGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTc                                                       >  2:210287/1‑150 (MQ=255)
                                                                                                    gggCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGctttg                                                      >  1:3981079/1‑148 (MQ=255)
                                                                                                    gggCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCg                                                      >  1:648691/1‑150 (MQ=255)
                                                                                                       cgcACCGCTTATTCTTCCCTGGTGGTGGCAGGCCAGTCGCTTTAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGACGGGTTTCGCT‑c                                                  <  2:1599740/150‑1 (MQ=255)
                                                                                                         cAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCT‑CCg                                                <  1:623572/150‑1 (MQ=255)
                                                                                                            cGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCT‑CCGGcc                                             <  1:3061347/150‑1 (MQ=255)
                                                                                                             gCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTGCT‑CCTGccc                                            >  2:197624/1‑149 (MQ=255)
                                                                                                              cTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCTCT‑CCGGCCtc                                           >  1:1923227/1‑149 (MQ=255)
                                                                                                               tGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCT‑CCTGCCCGt                                          >  2:1253649/1‑150 (MQ=255)
                                                                                                                gATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCT‑CCGGCCTGtt                                         <  1:1137832/150‑1 (MQ=255)
                                                                                                                gATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCT‑CCCGCCTGtt                                         >  1:3261600/1‑150 (MQ=255)
                                                                                                                gATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATCCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGCC‑CCCGcccggt                                         >  1:2092322/1‑146 (MQ=255)
                                                                                                                  tGCTTCCTTGGTGGTGGCGGGCCAGTCGCCTTAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCT‑CCGGCCTGTTTa                                       <  1:2282181/150‑1 (MQ=255)
                                                                                                                    ctaaacTGGTGGTGACCGACCGGTCTCGTAAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCTCTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCT‑CCGGCCTGTTTAcc                                     <  2:3990346/145‑1 (MQ=255)
                                                                                                                    cTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCT‑CCGGCCTGTTTAcc                                     <  1:3726296/150‑1 (MQ=255)
                                                                                                                      tACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTTCT‑CCCGGCTGTTTTcccc                                   >  2:2928452/1‑150 (MQ=255)
                                                                                                                      tACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGCT‑CCGGCCCGTTTAcccc                                   >  1:755099/1‑150 (MQ=255)
                                                                                                                      tACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGCT‑CCCGCCCCTTTTcccc                                   >  1:2107234/1‑150 (MQ=255)
                                                                                                                          tggtggtggCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTCCT‑CCGGCCCGTTTAtccctccc                               >  1:1751253/1‑150 (MQ=255)
                                                                                                                           ggtggtggCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCT‑CCGGTCCGTTTCTCCCtcgcc                              >  2:1736692/1‑146 (MQ=255)
                                                                                                                           ggtggtggCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCT‑CCGGCCCGTTTTTCCCCAccc                              >  1:221701/1‑149 (MQ=255)
                                                                                                                             tggtggCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGATATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCTCT‑CCGGCCCGTTTTcccccccccac                            >  2:2346813/1‑143 (MQ=255)
                                                                                                                              ggtggCGGGCCAGTCGCTTTATTTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCT‑CCGGCCTGTTTACCCCTACCGAAg                           <  1:2952582/150‑1 (MQ=255)
                                                                                                                                tggCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCT‑CCCGCCCGTTTTTCCCCACCCAAGcc                         >  1:2079222/1‑150 (MQ=255)
                                                                                                                                tggCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCT‑CCCCCCTGTTTTTCCCCACCGAAGcc                         >  1:2135004/1‑150 (MQ=255)
                                                                                                                                 ggCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTCCT‑TCCGCCCGTTTTTCCCCAACGCAGccc                        >  1:3910241/1‑149 (MQ=255)
                                                                                                                                       ccAGTCGCTTTAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCT‑CCGGCCTGTTTACCCCTACCGAAGCCGGGGCCg                  <  2:2553756/150‑1 (MQ=255)
                                                                                                                                        cAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTCGCT‑CCCGCCTGTTTTCCCCCACCCAAGCCGGGGCCgc                 >  1:1884439/1‑149 (MQ=255)
                                                                                                                                         aGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCT‑CCGGCCTGTTTGCCCATACCGAAGCCGGGGCCGtt                <  1:1422833/150‑1 (MQ=255)
                                                                                                                                          tgcttgttATTTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCT‑CCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTtg               <  2:1861390/144‑1 (MQ=255)
                                                                                                                                          gTCGCTTTAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCT‑CCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTtg               <  1:3314551/150‑1 (MQ=255)
                                                                                                                                          gTCGCCTTAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCT‑CCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTtg               <  1:755882/150‑1 (MQ=255)
                                                                                                                                          gTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCTCTCCCGGCCCGTTT‑CCCCTTCCCAAGCCggggggggtg               >  2:661421/1‑144 (MQ=255)
                                                                                                                                              ctttAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCT‑CCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTtgctgc           <  1:2908342/148‑1 (MQ=255)
                                                                                                                                              ctttAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCT‑CCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTtgctgc           <  1:1514718/148‑1 (MQ=255)
                                                                                                                                               tttAATTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCT‑CCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTgctgct          <  2:3476340/149‑1 (MQ=255)
                                                                                                                                                tgaaTTTC‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCT‑CCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCtt         <  1:2810220/148‑1 (MQ=255)
                                                                                                                                                 tAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCT‑CCGGCCCGTTTTTCCCCACCCACGCCCGGGCCGGTGCTGCttt        >  2:1873685/1‑150 (MQ=255)
                                                                                                                                                  aaTTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCCCC‑CCCTCCCGGTTACCCCTACCCAAACCCCGGCCGGTGCTGCTTTc       >  1:1561527/1‑150 (MQ=255)
                                                                                                                                                     ttA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCT‑CCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTAt    <  1:2025525/150‑1 (MQ=255)
                                                                                                                                                       a‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTTTCT‑CCCGCCCGTTTTCCCCTCCCCAAACCGGGGCCGGTGCTTCttttttttt  >  1:2551447/1‑144 (MQ=255)
                                                                                                                                                       a‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGGTTTGCT‑CCCGCCCGTTTTCCCCCACCCAAGCCGGGGCCGCTGCTGCttttttttc  >  1:2734004/1‑144 (MQ=255)
                                                                                                                                                       |                                                                                                                                                       
ATTATTCCCCCTTCCATTCCGTTTATTATCTTCGGCGTTTCCAGCGGATTATCCATCAGTAAGCTGTTTATGGCGGGCA‑TTG‑CCCCTGGCATGATGATGGGCGCAACGCTGATGCTTACCTGGTGGTGGCAGGCCAGTCGCCTTAATTTA‑CCCCGCCAGCAAAAAGCAACGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCT‑CCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGC  >  AP012306/3182712‑3183010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑