Predicted mutation
evidence seq id position mutation annotation gene description
RA AP012306 574,220 G→A A288V (GCG→GTG)  nagA ← N‑acetylglucosamine‑6‑phosphate deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306574,2200GA99.7% 1259.6 / NA 314A288V (GCG→GTG) nagAN‑acetylglucosamine‑6‑phosphate deacetylase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (141/172);  total (141/173)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 319 total aligned reads displayed.

AGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCA  >  AP012306/574073‑574366
                                                                                                                                                   |                                                                                                                                                  
  cGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACaaa                                                                                                                                                >  2:2905775/1‑150 (MQ=255)
  cGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACaaa                                                                                                                                                <  2:3805622/150‑1 (MQ=255)
   gTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACaaaa                                                                                                                                               >  1:2316152/1‑150 (MQ=255)
    tCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACaaaaa                                                                                                                                              <  2:4033448/150‑1 (MQ=255)
     cGCCATACGTAACACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGCTGCACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAAt                                                                                                                                             >  2:2826976/1‑150 (MQ=255)
      gCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATg                                                                                                                                            <  1:1328164/150‑1 (MQ=255)
        cATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGaa                                                                                                                                          <  1:2767658/150‑1 (MQ=255)
           aCGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTg                                                                                                                                       <  1:3735903/150‑1 (MQ=255)
            cGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGt                                                                                                                                      >  1:322083/1‑150 (MQ=255)
              tAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTc                                                                                                                                    >  2:734784/1‑150 (MQ=255)
               aGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCa                                                                                                                                   <  2:151763/150‑1 (MQ=255)
               aGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCa                                                                                                                                   >  2:3991563/1‑150 (MQ=255)
                gCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCaa                                                                                                                                  <  1:303611/150‑1 (MQ=255)
                  aCTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATg                                                                                                                                >  2:337361/1‑150 (MQ=255)
                  aCTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATg                                                                                                                                <  1:145632/150‑1 (MQ=255)
                    ttCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGtt                                                                                                                              <  1:1695705/150‑1 (MQ=255)
                     tCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTg                                                                                                                             >  2:602003/1‑150 (MQ=255)
                        tCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCa                                                                                                                          <  2:2800806/150‑1 (MQ=255)
                         ccAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCAc                                                                                                                         >  2:2124747/1‑150 (MQ=255)
                            gTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACctg                                                                                                                      <  1:155383/150‑1 (MQ=255)
                               cGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACctgctg                                                                                                                   <  2:510549/150‑1 (MQ=255)
                                 aTACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGgc                                                                                                                 <  1:549794/150‑1 (MQ=255)
                                   aCCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGgcgc                                                                                                               >  1:865336/1‑150 (MQ=255)
                                      gCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGt                                                                                                            >  1:3866495/1‑150 (MQ=255)
                                       cAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTg                                                                                                           >  2:1450116/1‑150 (MQ=255)
                                        aaTGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTgg                                                                                                          >  2:45168/1‑150 (MQ=255)
                                             tCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCa                                                                                                     <  1:3431161/150‑1 (MQ=255)
                                             tCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCa                                                                                                     <  2:2456815/150‑1 (MQ=255)
                                                   aGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAAcc                                                                                               <  2:1095807/150‑1 (MQ=255)
                                                     aTTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAg                                                                                             >  1:752216/1‑150 (MQ=255)
                                                      ttACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGa                                                                                            <  1:3480496/150‑1 (MQ=255)
                                                       tACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGac                                                                                           <  1:2856063/150‑1 (MQ=255)
                                                       tACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGac                                                                                           >  1:2751221/1‑150 (MQ=255)
                                                       tACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGac                                                                                           >  2:2271179/1‑150 (MQ=255)
                                                        acgcacgcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGaca                                                                                          >  2:643297/1‑150 (MQ=255)
                                                          gcacgcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGAcaca                                                                                        >  2:1814570/1‑150 (MQ=255)
                                                            acgcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGt                                                                                      <  1:1805354/150‑1 (MQ=255)
                                                                 ttCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTc                                                                                 <  2:3620824/150‑1 (MQ=255)
                                                                 ttCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTc                                                                                 >  2:1289065/1‑150 (MQ=255)
                                                                     aTCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCt                                                                             <  2:3074543/150‑1 (MQ=255)
                                                                      tCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCtt                                                                            <  1:2502270/150‑1 (MQ=255)
                                                                       cATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCttt                                                                           <  2:2551231/150‑1 (MQ=255)
                                                                       cATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCttt                                                                           >  2:3231515/1‑150 (MQ=255)
                                                                          ggTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAg                                                                        >  1:931220/1‑150 (MQ=255)
                                                                          ggTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAg                                                                        <  2:1877915/150‑1 (MQ=255)
                                                                               aGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGtt                                                                   <  2:2998005/150‑1 (MQ=255)
                                                                                 gATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTa                                                                 <  2:3395229/150‑1 (MQ=255)
                                                                                  aTGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAg                                                                <  1:422639/150‑1 (MQ=255)
                                                                                   tGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGc                                                               <  2:2692196/150‑1 (MQ=255)
                                                                                     aaCCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGt                                                             >  1:2816494/1‑150 (MQ=255)
                                                                                         gCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCg                                                         <  1:3255340/150‑1 (MQ=255)
                                                                                         gCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCg                                                         <  1:2829628/150‑1 (MQ=255)
                                                                                           ttAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGaa                                                       <  1:1737895/150‑1 (MQ=255)
                                                                                             aaCGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGATCTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATg                                                     <  2:2778458/150‑1 (MQ=255)
                                                                                             aaCGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATg                                                     <  1:3815536/150‑1 (MQ=255)
                                                                                              aCGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGt                                                    >  2:558908/1‑150 (MQ=255)
                                                                                                 tACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTgg                                                 <  1:1008820/150‑1 (MQ=255)
                                                                                                  aCCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGc                                                >  2:1979514/1‑150 (MQ=255)
                                                                                                   ccGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCg                                               <  2:2177100/150‑1 (MQ=255)
                                                                                                      ttCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTaa                                            <  1:2633320/150‑1 (MQ=255)
                                                                                                        ctcATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATc                                          <  2:2490331/150‑1 (MQ=255)
                                                                                                         tcATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCa                                         <  1:2993012/150‑1 (MQ=255)
                                                                                                          cATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCaa                                        <  1:2353397/150‑1 (MQ=255)
                                                                                                           aTCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAAc                                       >  1:3447860/1‑150 (MQ=255)
                                                                                                             ccACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACAt                                     <  1:3126369/150‑1 (MQ=255)
                                                                                                              cacacaAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATg                                    <  2:1368780/150‑1 (MQ=255)
                                                                                                               acacaAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGc                                   <  1:2608916/150‑1 (MQ=255)
                                                                                                                 acaAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAg                                 >  2:2138904/1‑150 (MQ=255)
                                                                                                                 acaAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAg                                 >  2:3530654/1‑150 (MQ=255)
                                                                                                                 acaAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAg                                 >  1:3322270/1‑150 (MQ=255)
                                                                                                                  caAAGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAgg                                >  1:2237206/1‑150 (MQ=255)
                                                                                                                    aaGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGcc                              <  1:2091358/150‑1 (MQ=255)
                                                                                                                     aGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCa                             >  1:107798/1‑150 (MQ=255)
                                                                                                                     aGTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCa                             >  1:1571363/1‑150 (MQ=255)
                                                                                                                      gTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCAt                            <  2:65820/150‑1 (MQ=255)
                                                                                                                      gTCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCAt                            <  2:1606213/150‑1 (MQ=255)
                                                                                                                       tCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATc                           >  1:4022639/1‑150 (MQ=255)
                                                                                                                       tCCGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGAGCGGTGGCGTCTGTAACCCGACACAGTTTGTCGCCTTTCAGCCGTTTAGCGTTGCGAATGTTATCGTAATCAACATGCAGGCCATc                           >  2:274865/1‑150 (MQ=255)
                                                                                                                         cGTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAg                         >  2:1017005/1‑150 (MQ=255)
                                                                                                                          gTTACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGc                        <  2:1159364/150‑1 (MQ=255)
                                                                                                                           ttACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCa                       >  2:2645508/1‑150 (MQ=255)
                                                                                                                           ttACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCa                       <  2:3970443/150‑1 (MQ=255)
                                                                                                                            tACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCaa                      <  1:582128/150‑1 (MQ=255)
                                                                                                                            tACGGTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCaa                      >  2:230167/1‑150 (MQ=255)
                                                                                                                               ggTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCaataa                   <  1:452564/150‑1 (MQ=255)
                                                                                                                               ggTAGTATATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCaataa                   <  1:4008305/150‑1 (MQ=255)
                                                                                                                                gtagtaTATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCaataat                  >  1:2011821/1‑150 (MQ=255)
                                                                                                                                 tagtaTATTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAataata                 >  1:2694777/1‑150 (MQ=255)
                                                                                                                                    tataTTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCg              <  1:648901/150‑1 (MQ=255)
                                                                                                                                     atatTGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGc             >  2:374735/1‑150 (MQ=255)
                                                                                                                                         tGTTTTACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATa         <  1:3361452/150‑1 (MQ=255)
                                                                                                                                           ttttACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATaaa       >  2:3891355/1‑150 (MQ=255)
                                                                                                                                           ttttACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATaaa       <  1:2156431/150‑1 (MQ=255)
                                                                                                                                            tttACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAAt      <  2:172137/150‑1 (MQ=255)
                                                                                                                                            tttACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAAt      >  2:3665248/1‑150 (MQ=255)
                                                                                                                                             ttACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATg     <  2:1675554/150‑1 (MQ=255)
                                                                                                                                              tACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGt    <  1:3664924/150‑1 (MQ=255)
                                                                                                                                              tACCCACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGt    <  1:3275913/150‑1 (MQ=255)
                                                                                                                                                cccACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCa  <  1:511619/150‑1 (MQ=255)
                                                                                                                                                cccACAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCa  <  2:1388094/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCA  >  AP012306/574073‑574366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑