Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,066,3230AC31.8% 547.9 / 42.6 243intergenic (‑92/+33)ychM/prsApredicted transporter/phosphoribosylpyrophosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (150/15);  new base C (0/77);  total (150/93)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 274 total aligned reads displayed.

AGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGC  >  AP012306/1066174‑1066471
                                                                                                                                                     |                                                                                                                                                    
aGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACaaaaaaa                                                                                                                                                      <  1:4422386/150‑1 (MQ=255)
     gCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACccgc                                                                                                                                                 >  1:4007277/1‑150 (MQ=255)
     gCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACccgc                                                                                                                                                 <  2:4809786/150‑1 (MQ=255)
       gTCGATCAGAGCGCGGAAAGGCGTCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACccgccg                                                                                                                                               >  2:2819009/1‑150 (MQ=255)
       gTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACccgccg                                                                                                                                               >  2:3832782/1‑150 (MQ=255)
       gTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACccgccg                                                                                                                                               >  1:3196870/1‑150 (MQ=255)
       gTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGCGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACccgccg                                                                                                                                               >  1:4464870/1‑150 (MQ=255)
            tCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCg                                                                                                                                          >  1:4171201/1‑150 (MQ=255)
              agagCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCggg                                                                                                                                        >  1:2861819/1‑150 (MQ=255)
               gagCGCGGAAAGGCATCACATGTGAGAAAAATATTTTGTTCAAAATAGTGTTTCATCCGTGAGCGCATCATCTGTAAATAAAAGGGCGGGTGAGGGGGGCATAGGTCATACAAATGAATATAACAGAAAAAAAACCCCGCCGCAGCGGGt                                                                                                                                       <  2:4356200/150‑1 (MQ=255)
                  cgcgGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCtt                                                                                                                                    >  1:3605097/1‑150 (MQ=255)
                      gAAAGGCATCACATGGGAGAAAAATTTTTTGTTCAAAATAATGTTTCACCGGTGGGCGCATCATCTGTAAACAAAAGGGCAGGTGAGGGAGGCATGGTTCATAAAAAGGGATATTACAAAAAAAAAAACCCGCCGCAGCGGGTCTTTGAg                                                                                                                                <  2:1535238/150‑1 (MQ=255)
                       aaaGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGCGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGc                                                                                                                               >  2:3292225/1‑150 (MQ=255)
                           gCATCACATGTGAGGAAAATATTTTGTTCAAAATAATGTTTCACCCGTGAGCGCATCATCTGTAAACAAAAGGGCAGGTGAGGGAGGAATAGGTCATAAAAATGGATTTTACAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCggg                                                                                                                           <  2:334432/150‑1 (MQ=255)
                            caccacaGGGGAGAAAAATTTTTTGTTCAAAATATTGTTTCATCCGTGAGCGCATCATCTGCCAACAAAAGGGCGGGTGAGGGAGGCATGGGTAATAAAAAGGGATATTACAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGt                                                                                                                          <  2:4108210/147‑1 (MQ=255)
                               cacaTGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCg                                                                                                                       >  2:613457/1‑150 (MQ=255)
                                  aTGTGAGAAAAATTTTTTGTTAAAAATAATTTTTAATCCGTGAGCGCATCATTTGACAAAAAAAGGGCAGGTGAAGGAGGCATGGGTCAAAAAAATGGATATTACAGAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGAtt                                                                                                                    <  2:4484433/150‑1 (MQ=255)
                                  aTGTGAGAAAAATTTTTTGTCCAAAATAATGTTTCACCGGTGAGCGCATCATTTGTCAACAAAATGGCGGGTGAGGGAGGCATAGGTAAAACAAATGGTTATAACAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGAtt                                                                                                                    <  2:22078/150‑1 (MQ=255)
                                   tgtgAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTa                                                                                                                   >  1:1772703/1‑150 (MQ=255)
                                    gtgAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAg                                                                                                                  >  2:3461341/1‑150 (MQ=255)
                                     tgAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAgt                                                                                                                 >  1:4735482/1‑150 (MQ=255)
                                     tgAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAgt                                                                                                                 >  2:2757949/1‑150 (MQ=255)
                                         gAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTc                                                                                                             >  1:2552153/1‑150 (MQ=255)
                                           aaaTATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGa                                                                                                           >  2:371751/1‑150 (MQ=255)
                                             attttttGTAAAAAATAATGTTTCATCGGTGAGCGCATCATCGGCCAACAAAAGGGCAGGGGAGGGAGGGATGGGTAATAAAAATGTATATTACAGAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAAc                                                                                                         <  2:1321169/147‑1 (MQ=255)
                                             atatTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAAc                                                                                                         >  1:3892225/1‑150 (MQ=255)
                                             atatTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTCCGATTAGTGTTCGAAc                                                                                                         >  2:3788808/1‑150 (MQ=255)
                                                   tGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCa                                                                                                   >  1:1048199/1‑150 (MQ=255)
                                                   tGTCCACAATAATGTTTCTTCCGGGGGCCAATCTCTTGAAAAAAAAATGGAGGGTGAAGGGGGCATAGGTAATAAAAAGGGATATTAAAGAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCa                                                                                                   <  2:2313200/150‑1 (MQ=255)
                                                    tttCAAAATATTTTTTCATCCGTGAGCGCATCATCTGTCAACAAAAGGGAAGGGGAAGGAGGCATAGGTCAAAAAAAGGGATATTACAGAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCag                                                                                                  <  2:3660842/149‑1 (MQ=255)
                                                     ttCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCaga                                                                                                 >  1:3295360/1‑150 (MQ=255)
                                                        acaATAATTTTTCATCCGTGAGCGCATCATCTGTAAACTAAATGGCAGGTGAAGGAGGCATAGGTCATAAAAATGGATATTACAGAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGAt                                                                                              <  2:2472561/150‑1 (MQ=255)
                                                        acaATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGAt                                                                                              >  1:2249532/1‑150 (MQ=255)
                                                         caATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATc                                                                                             >  1:3817117/1‑150 (MQ=255)
                                                         caATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATc                                                                                             >  2:858635/1‑150 (MQ=255)
                                                           ataatGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGCGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGa                                                                                           >  1:511811/1‑150 (MQ=255)
                                                            taatGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAt                                                                                          >  1:3060650/1‑150 (MQ=255)
                                                             aatGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGATGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCGGCGCCGGCGGGTCTTTGGCCCGGGTTGGCTTAGGGTTCGAACATGGCAGGGATCGAtt                                                                                         >  2:1731338/1‑150 (MQ=255)
                                                              atGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAttc                                                                                        >  1:2481609/1‑150 (MQ=255)
                                                                ttttCCTCTGTGAGCGTATCTTATGTCAATAAAAGGGCAGGTGAAGGGGGCATAGGTCATACAATTGGATATTACAAACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAttctt                                                                                      <  2:4667754/149‑1 (MQ=255)
                                                                 tttCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAttcttc                                                                                     >  2:4746962/1‑150 (MQ=255)
                                                                 tttCATCCGTGAGCGAATCATCTGCCAACTAAATGGGAGGTGAAGGAGGCATAGGTCATAAAAAGGGATTTTAAAGAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAttcttc                                                                                     <  2:361337/150‑1 (MQ=255)
                                                                 tttCACCGGTGAGCGAATCATCTGTCAACAAAATGGCAGGTGAAGGGGGCATAGGTCATAAAAATGGATATTACAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAttcttc                                                                                     <  2:3995753/150‑1 (MQ=255)
                                                                   tCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGt                                                                                   >  1:1676556/1‑150 (MQ=255)
                                                                    cATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGtt                                                                                  >  1:3116128/1‑150 (MQ=255)
                                                                    cATCCGTGAGCGCATCATCTGTAAACAAAATGGCGGGTGAGGGGGGCATGGTTCATAAAAATGGATATTACAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGtt                                                                                  <  2:4024489/150‑1 (MQ=255)
                                                                       ccGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCt                                                                               >  1:2019911/1‑150 (MQ=255)
                                                                          tGAGCACATCATCTGTCAACAAAATGGCGGGTGAAGGAGGCATAGTTCATAAAAAGGGATATTAAAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGAt                                                                            <  2:2870896/150‑1 (MQ=255)
                                                                           gAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATa                                                                           >  1:907563/1‑150 (MQ=255)
                                                                            aGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATac                                                                          >  1:1349118/1‑150 (MQ=255)
                                                                              aaatttatcTGTAAACAAAAGGGAAGGTAAGGGGGGAATAGTTAATAAAAATGGTTTGAAAAGAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATacga                                                                        <  2:1381738/144‑1 (MQ=255)
                                                                               gcATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTTATacgac                                                                       >  1:1146432/1‑150 (MQ=255)
                                                                               gcATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATacgac                                                                       >  2:3163306/1‑150 (MQ=255)
                                                                               gcATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATacgac                                                                       >  2:2687052/1‑150 (MQ=255)
                                                                                  tcatcTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGaa                                                                    >  1:2739901/1‑150 (MQ=255)
                                                                                    atcTGAAAACAAAAGGGCAGGTGAAGGAGGCATAGGTAATAAAAATGAATATTACAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATc                                                                  <  2:1807084/150‑1 (MQ=255)
                                                                                       tGTCAACTAAATGGCAGGTGAAGGAGGCATGGGTCATACAAATGGATATTACAGAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCt                                                               <  1:2844677/150‑1 (MQ=255)
                                                                                       tGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCt                                                               >  1:2202192/1‑150 (MQ=255)
                                                                                        gacaaaaaaaGGGCAGGAGAGGGAGGAATAGGTAATAAAAATGAATATTAAAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCtt                                                              <  2:9561/143‑1 (MQ=255)
                                                                                          cAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCg                                                            >  2:2715540/1‑150 (MQ=255)
                                                                                           aaCTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCgg                                                           >  2:2772327/1‑150 (MQ=255)
                                                                                           aaCTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCgg                                                           >  2:3242642/1‑150 (MQ=255)
                                                                                               aaaTGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAg                                                       >  2:2644713/1‑150 (MQ=255)
                                                                                                aagggCAGGTGAAGGAGGCATAGGTCATAAAAATGGATATTACAGACAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGc                                                      <  2:4324824/147‑1 (MQ=255)
                                                                                                  gggaaGGTGAAGGAGGAATAGGTCATAAAAATGGATATTACAGAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGAAt                                                    <  1:373053/146‑1 (MQ=255)
                                                                                                  gggCGGGAGAGGGAGGCATAGGAAATAAAAAGGAATATAAAAGACAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCAt                                                    <  1:4842553/149‑1 (MQ=255)
                                                                                                  gggCAGGTGAGGGAGGAATAGGTCATAAAAATGGATATTACAAACAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCAt                                                    <  1:67/149‑1 (MQ=255)
                                                                                                   ggCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATa                                                   >  1:2350210/1‑150 (MQ=255)
                                                                                                       ggggAAGGAGGCATAGGTAAAAAAAATGGATATTAAAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTg                                               <  2:1591505/147‑1 (MQ=255)
                                                                                                            ggggggCATAGGTCATAAAAAGGGATATTACAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAgg                                          <  2:4384004/146‑1 (MQ=255)
                                                                                                            aggaggCATGGGTAATAAAAATGGATATTACAAAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAgg                                          <  2:2248229/150‑1 (MQ=255)
                                                                                                              gaggCATGGGTCATAAAAATGGATATTAAAGACAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGt                                        <  1:1237551/150‑1 (MQ=255)
                                                                                                              gaggCATAGGTCAAAAAAATGGATATAACAAAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCCGACAGGGt                                        <  2:3388103/150‑1 (MQ=255)
                                                                                                               gggaatgggTCAAAAAAAGGGATATTAAAAAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTc                                       <  2:123616/143‑1 (MQ=255)
                                                                                                               aggCATAGGTCAAAAAAAGGAATATTACAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTc                                       <  2:4521760/150‑1 (MQ=255)
                                                                                                                   aTAGGTCATAAAAAGGGATATTAAAAACAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCagag                                   <  2:255321/150‑1 (MQ=255)
                                                                                                                      ggTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAACCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTAc                                >  2:2491626/1‑150 (MQ=255)
                                                                                                                      ggTAATACAAATGGATATTAAAGAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTAc                                <  1:4263141/150‑1 (MQ=255)
                                                                                                                       gTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGCGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCACCATACCTGACAGAGTCAGACTACg                               >  2:685590/1‑150 (MQ=255)
                                                                                                                         caaaCAAATGGATATTAAAAAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCa                             <  2:1294066/147‑1 (MQ=255)
                                                                                                                           tACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACg                           >  1:3401321/1‑150 (MQ=255)
                                                                                                                            aCAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGt                          >  2:3046964/1‑150 (MQ=255)
                                                                                                                             aaaaTGGATATTACAAAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGtt                         <  2:1255340/149‑1 (MQ=255)
                                                                                                                              aaaTGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGGGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTc                        >  1:3499927/1‑150 (MQ=255)
                                                                                                                               aaTGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCg                       >  2:1128040/1‑150 (MQ=255)
                                                                                                                               aaTGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCg                       >  2:1916340/1‑150 (MQ=255)
                                                                                                                               aaTGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGGCAGAGTACGCACGTTCg                       >  1:1382592/1‑150 (MQ=255)
                                                                                                                               aaTGAATATAAAAAAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCg                       <  2:327228/150‑1 (MQ=255)
                                                                                                                                   gATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAg                   >  2:184579/1‑150 (MQ=255)
                                                                                                                                    atatTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGt                  >  1:4573676/1‑150 (MQ=255)
                                                                                                                                      atTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtga                >  1:291408/1‑150 (MQ=255)
                                                                                                                                       ttACAGAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtgat               <  1:1493548/150‑1 (MQ=255)
                                                                                                                                          cAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCAAGTTCGGCAGtgatttg            <  1:2619508/150‑1 (MQ=255)
                                                                                                                                              aaaaaaaaCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTgatttgattt        <  2:2984022/149‑1 (MQ=255)
                                                                                                                                               aaaaaaaCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTc       >  2:1003882/1‑150 (MQ=255)
                                                                                                                                               aaaaaaaCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTc       >  2:3028058/1‑150 (MQ=255)
                                                                                                                                                 aaaacCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCAt     <  2:4645414/150‑1 (MQ=255)
                                                                                                                                                  aaaaCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATc    >  2:3755140/1‑150 (MQ=255)
                                                                                                                                                    aaCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGc  >  1:2141093/1‑150 (MQ=255)
                                                                                                                                                    aaCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGc  >  1:1955577/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTACAGACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGC  >  AP012306/1066174‑1066471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑