Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,903,7910AG23.7% 538.3 / 61.2 228E189G (GAA→GGA) yjgJpredicted transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (29/145);  new base G (54/0);  total (83/145)
Fisher's exact test for biased strand distribution p-value = 1.95e-31
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 327 total aligned reads displayed.

ACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTT  >  AP012306/3903643‑3903940
                                                                                                                                                    |                                                                                                                                                       
aCCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGaa                                                                                                                                                        <  2:4050886/150‑1 (MQ=255)
 ccATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAg                                                                                                                                                       >  1:1634455/1‑150 (MQ=255)
  cATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGc                                                                                                                                                      >  2:3943889/1‑150 (MQ=255)
  cATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGc                                                                                                                                                      <  1:1441280/150‑1 (MQ=255)
   aTTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCg                                                                                                                                                     >  2:2518103/1‑150 (MQ=255)
      tATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATa                                                                                                                                                  >  2:334583/1‑150 (MQ=255)
       aTGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATaa                                                                                                                                                 >  1:3469181/1‑150 (MQ=255)
        tGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATaaa                                                                                                                                                <  2:4365247/150‑1 (MQ=255)
         gACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGGTaaaa                                                                                                                                               >  2:3861511/1‑150 (MQ=255)
         gACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGACCGCTaaaa                                                                                                                                               >  1:2540069/1‑150 (MQ=255)
         gACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAACGATaaaa                                                                                                                                               >  2:1463879/1‑150 (MQ=255)
         gACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATaaaa                                                                                                                                               <  1:3438626/150‑1 (MQ=255)
          aCTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATaaaaa                                                                                                                                              >  1:4314811/1‑150 (MQ=255)
          aCTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATaaaaa                                                                                                                                              <  1:1984117/150‑1 (MQ=255)
            tACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATaaaaaat                                                                                                                                            >  2:3627864/1‑148 (MQ=255)
              cATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAatat                                                                                                                                          >  1:1538381/1‑150 (MQ=255)
               aTCGCCAGGCGGCATCCACAACGCGCACAATGTGGGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGCGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGTAAGAAAATAtt                                                                                                                                         >  2:727513/1‑150 (MQ=255)
                  gCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGAAAAAAACTATtttt                                                                                                                                      >  2:3308328/1‑150 (MQ=255)
                    cAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTga                                                                                                                                    <  2:1678561/150‑1 (MQ=255)
                       gcggcATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTgagga                                                                                                                                 <  1:180902/150‑1 (MQ=255)
                          gcATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTTAGGAGTg                                                                                                                              >  1:4383439/1‑150 (MQ=255)
                          gcATCCACAACACGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTg                                                                                                                              <  1:504075/150‑1 (MQ=255)
                              ccACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑Gc                                                                                                                         <  2:3222183/150‑1 (MQ=255)
                                  aaCGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGGGAAAAAATATTCTTTTGGAGGGAT‑Gggggg                                                                                                                     >  2:4419145/1‑149 (MQ=255)
                                     gcgcACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTGAGGGGGGGT‑GCggggcct                                                                                                                  >  2:4247026/1‑146 (MQ=255)
                                      cgcACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAAATATTTTTTAGGAGTGAT‑GCGGGCCTTg                                                                                                                 >  1:2097810/1‑150 (MQ=255)
                                        cacaATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGGTAAAAAATATTCTTTAGGAGGGAT‑GCGGGGCTTGAc                                                                                                               >  2:3528417/1‑150 (MQ=255)
                                        cacaATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGAc                                                                                                               <  2:654200/150‑1 (MQ=255)
                                         acaATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGATAAAAACCATTATTTAGAAGCGAA‑TCCGGCCTTTAcc                                                                                                              >  1:2401193/1‑150 (MQ=255)
                                         acaATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGAGAAAAAAAATTTTTTAGGGGAGGA‑GCGGGCCCTGGcc                                                                                                              >  2:54606/1‑150 (MQ=255)
                                          gaaTGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGAccc                                                                                                             <  2:1780766/149‑1 (MQ=255)
                                          caATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTGAGGAGGGAT‑GCGGGGCTTGAccc                                                                                                             >  1:2187638/1‑150 (MQ=255)
                                          caATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGAccc                                                                                                             <  2:4377847/150‑1 (MQ=255)
                                              gtgtgACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGGGCGAGAAAAAATATTCTTGAGGAGGGAT‑GCGGGGCTTGACGCG‑aaa                                                                                                        >  2:1335229/1‑150 (MQ=255)
                                              gtgtgACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTTAGGAGTGAT‑GCGGGGCTTGACCCG‑aaa                                                                                                        >  2:1875434/1‑150 (MQ=255)
                                                       aTTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGaaa                                                                                               <  1:1739928/150‑1 (MQ=255)
                                                         tttATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAgc                                                                                             <  2:849722/150‑1 (MQ=255)
                                                           tATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAAAATTTTTTAGGAGGGGTGGCGGGCCTGACCCCG‑AAAGCGGGGaaaagc                                                                                            >  2:2212551/1‑147 (MQ=255)
                                                           tATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAAAATTTTTGAAGAAGGGT‑GCGGGGCTTGACCCC‑AAAGGCGGGaaaaggc                                                                                           >  2:1567989/1‑146 (MQ=255)
                                                            aTGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGcc                                                                                          <  2:2083234/150‑1 (MQ=255)
                                                              gAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAACGATAAAAAATATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAAGCGGGGAAACGCCTg                                                                                        >  1:1007401/1‑150 (MQ=255)
                                                              gAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTg                                                                                        <  1:2462614/150‑1 (MQ=255)
                                                                gTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCC‑AAAAGCGGGGAAAcgcccgcc                                                                                      >  1:2581109/1‑150 (MQ=255)
                                                                gTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGcc                                                                                      <  1:1379336/150‑1 (MQ=255)
                                                                 tACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTTTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGGAAACGCCTGCCg                                                                                     >  1:2624619/1‑150 (MQ=255)
                                                                 tACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCg                                                                                     <  1:3291013/150‑1 (MQ=255)
                                                                       gATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTGTGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGGAAACGCCCGCCGGTAAGt                                                                               >  2:3741330/1‑150 (MQ=255)
                                                                        aTGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTTAGGAGGGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGGAAGCGCCCGCCGATAAGtt                                                                              >  2:4152227/1‑150 (MQ=255)
                                                                        aTGTCGGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACACCTCTGTGCAACAGCTGCAATGGCGGGGGAGGCGATAAAAACTATTCTTGGGGGGTGAT‑GCGGGCCTTGCCCCG‑AAAGCCGGGAACGCCCCTGCCGATAAGtt                                                                              <  2:1836346/150‑1 (MQ=255)
                                                                          gTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGGTAAAAAATATTTTTTGGGAGGGGT‑GGGGGGCCTGACCCGCAAAGGGGGGGAA‑CGCCTGCCGAGAAGTtgt                                                                            >  2:1133522/1‑150 (MQ=255)
                                                                             tGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGCCCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGAt                                                                         <  1:192210/150‑1 (MQ=255)
                                                                              gggCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTGAGGAGGGAT‑GCGGGCCCTGACCCG‑AAAGGCGGGGAAACGCCCGCCGGTAAATTGTGGTa                                                                        >  2:3668831/1‑150 (MQ=255)
                                                                              gggCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATa                                                                        <  1:1089171/150‑1 (MQ=255)
                                                                               ggCTTTCTGCGAAGGCACGAGAGGGGCCCTCTATAGAACAACTCTGTGCAACAGCTGCAATGGGGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGCCCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATaa                                                                       <  2:230275/150‑1 (MQ=255)
                                                                                   ttCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGAACCAGCTGCAAGGGCGGGGGAAGCGATAAAAGCTATTCTTGAGGAGTGGT‑GCGGGCCTTGACCCG‑AAAGGCGGAAAAGCGCCTGCCGATAAGTTGTGATAAGACa                                                                   <  2:2776827/150‑1 (MQ=255)
                                                                                    tCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGATAAAAAAAATTTTTTAGGGGGGGT‑GCGGGGCTTGACCCG‑AAAGGGGGGGAAAAGCCCGCCGGTAAGATGTGATaaaaaaa                                                                  >  2:2399172/1‑145 (MQ=255)
                                                                                      tGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACaata                                                                <  2:893125/150‑1 (MQ=255)
                                                                                        cGAAGGCAGGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCTTTGACCCG‑AAAGGGGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACaataat                                                              <  2:577003/150‑1 (MQ=255)
                                                                                          aaGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAAGGGGGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCTTTGACCCG‑AAAGGCGGGAAGGCGCCTGCCGATAAGTTGTGATAAGACAATAATTc                                                            <  2:499991/150‑1 (MQ=255)
                                                                                           aGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAGGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCa                                                           <  1:791801/150‑1 (MQ=255)
                                                                                              cACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGGGATAAAAAATATTTTTGAGGGGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGGAAACGCCCGCCGATAAGGTGTGATAAGACAAAAATTTAcgc                                                        >  2:1908935/1‑150 (MQ=255)
                                                                                              cACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCAcgc                                                        >  1:4145459/1‑150 (MQ=255)
                                                                                               aCGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCAcgcg                                                       <  1:317074/150‑1 (MQ=255)
                                                                                                 gagaGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAAGGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGtt                                                     <  2:3903556/150‑1 (MQ=255)
                                                                                                  agggggtCACTGTATAGAACACCGCTGAGCACCCGCCGCAATGGCGGGGGACGCGATAAAAAATATTGTTTAGGAGCGAT‑GCGGGCGTCAACCCA‑AAAGGGGGGAACGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTa                                                    <  2:2596368/147‑1 (MQ=255)
                                                                                                   gagGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTaa                                                   <  1:2883239/150‑1 (MQ=255)
                                                                                                   gagGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAG‑GCGGGCCTTGACCCG‑AAGGGGGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTaa                                                   <  2:908957/150‑1 (MQ=255)
                                                                                                        gCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTa                                              <  2:1713349/150‑1 (MQ=255)
                                                                                                        gCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTa                                              <  1:3793681/150‑1 (MQ=255)
                                                                                                          aCTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTTAGGGGGGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAACGCCTGCCGATAAGTTGTGGTAAGACAAAAAATCACGCGGTAAGGCTAGc                                            >  2:4107869/1‑150 (MQ=255)
                                                                                                               ataGAACAACTGTGTGCAACAGCTGAAATGGCGGGGGAAGGGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAAt                                       <  1:4232911/150‑1 (MQ=255)
                                                                                                               ataGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAAt                                       <  2:2948474/150‑1 (MQ=255)
                                                                                                               ataAAACACCTTTGTGCAACAGCTGCAAGGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCA‑AAGGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAAt                                       <  2:3397949/150‑1 (MQ=255)
                                                                                                                taGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGCGAT‑GCGGGCCTTGACCCG‑AAAGGGGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAAtt                                      <  2:153831/150‑1 (MQ=255)
                                                                                                                 aGAACACCTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTg                                     <  2:1363031/150‑1 (MQ=255)
                                                                                                                   aacaacTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAGGGGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGAt                                   <  1:932728/150‑1 (MQ=255)
                                                                                                                     caacTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTTTTTAGGGGGGAT‑GCGGGGCTTGGCCCG‑AAAGGGGGGGAAACGCCCGCCGATAAGTTGTGATAAAACAATAAATCACGCGTTAAGGGTAGCGGGATTGGTTa                                 >  2:2256046/1‑150 (MQ=255)
                                                                                                                            gtgCAACAGCTGCAATGGCGGGGGGAGCGATAAAAATTATTATTGAGGAGGGGT‑GTGGGGCGTTGCCCG‑AAAAGTGGGGAAATGCCAGCCGAGAAATTGGGATAAGAACAAAAATCACGGGGTTAAGCTTTCGGGATTTATttttttttt                          >  2:703225/1‑149 (MQ=255)
                                                                                                                            gtgCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTc                          <  1:1064908/150‑1 (MQ=255)
                                                                                                                            gtgCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTc                          <  2:3150748/150‑1 (MQ=255)
                                                                                                                             tgCAACAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTTTTTTGGGGGGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGGAAAAGCCCGCCGATAAGTTGTGATAAAACAAAAATTCACGCGGTAAGGCTAGCGGAATTGATTATCtttttg                         >  2:1980843/1‑148 (MQ=255)
                                                                                                                                aaCAGCTGCAATGGCGGGGGGAGGGAAAAAAAAAATTTTTTTGGGGGGAG‑GCGGGCCTTGACCCG‑AAAGGCGGGGAAGGGGAGGCCCGGAAATTTTGATAAGACAAAAAATTACGCGGTTAGGCTAGGGGAAGTTATTATCTTTTTGttt                      >  2:1286246/1‑148 (MQ=255)
                                                                                                                                 aCAGCTGCAATGGCGGGGGGAGCGATAAAAAATATTCTTGAGGAGGGAT‑GCGGGCCTTGACCCC‑AAAAGCGGGGAAAAGCCTGCCGATAAGTTGTGGTAAAACAAAAATTTACGCGTTAAAGGTAGCGGGAATGATTATTTTTTTGttta                     >  2:2865048/1‑150 (MQ=255)
                                                                                                                                 aCAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGtata                     <  1:3862816/150‑1 (MQ=255)
                                                                                                                                  cAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCTTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATaa                    <  1:2267004/150‑1 (MQ=255)
                                                                                                                                  cAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATaa                    <  2:3805035/150‑1 (MQ=255)
                                                                                                                                  cAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATaa                    <  2:2821144/150‑1 (MQ=255)
                                                                                                                                  cAGCTGCAAGGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATaa                    <  2:967184/150‑1 (MQ=255)
                                                                                                                                   aGCTGCAATGGCGGGGGGAGGGATAAAAAATATTTTTTAGGAGGGAT‑GGGGGCCTTGACCCG‑AAAGGGGGGGAAAGGCCTGCCGATAAGTTGTGATAAGACAAAAAATCACGCGTTAAGGGTAGCGGAATTGATTATCTTTTTGTATAAc                   >  2:2696682/1‑150 (MQ=255)
                                                                                                                                     cTGCAATGGCGGGGGAAGCGATAAAAAATATTCTTTAGGAGGGAT‑GCGGGGCTTGACCCG‑AAAAGCGGGAAAACGCCTGCCGATAAAATGTGATAAAACAAAAAATTACCCGTTAAAGCTAGCGGAAATTATTATTTTTTTTTATAAAGa                 >  2:1850958/1‑150 (MQ=255)
                                                                                                                                      tGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGAt                <  1:4504161/150‑1 (MQ=255)
                                                                                                                                        aaaTGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAg              <  1:3974094/149‑1 (MQ=255)
                                                                                                                                           tGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGaaa           >  1:3126757/1‑150 (MQ=255)
                                                                                                                                             gCGGGGGAAGCGATAATAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATg         <  1:2728329/150‑1 (MQ=255)
                                                                                                                                              cGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGGATAACGATAGAAATGa        >  2:4314081/1‑150 (MQ=255)
                                                                                                                                                ggggAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGaaa      >  2:455729/1‑150 (MQ=255)
                                                                                                                                                ggggAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGaaa      >  1:2951352/1‑150 (MQ=255)
                                                                                                                                                 gggAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAAc     <  1:3655513/150‑1 (MQ=255)
                                                                                                                                                    aaGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACgtt  <  1:1166201/150‑1 (MQ=255)
                                                                                                                                                    aaGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACgtt  <  2:3022074/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                       
ACCATTTATGACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAT‑GCGGGCCTTGACCCG‑AAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTT  >  AP012306/3903643‑3903940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑