Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123063,938,8870CT29.7% 441.4 / 160.4 240S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (20/148);  new base T (71/0);  total (92/148)
Fisher's exact test for biased strand distribution p-value = 8.28e-43
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 303 total aligned reads displayed.

CTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACG  >  AP012306/3938739‑3939036
                                                                                                                                                     |                                                                                                                                                      
  cATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑Atttttttttt                                                                                                                                                     >  1:1591000/1‑150 (MQ=255)
  cATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATtttttcttt                                                                                                                                                     <  2:3613137/150‑1 (MQ=255)
  cATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGC‑CTtttttcttt                                                                                                                                                     >  2:2384136/1‑150 (MQ=255)
     aGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGCC‑Tttttttttttttt                                                                                                                                                  >  2:1467892/1‑149 (MQ=255)
     aGAAAAATGCGTCATGAGTAGTATAGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑Attttttttttttt                                                                                                                                                  >  1:1176986/1‑149 (MQ=255)
       aaaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑Tg                                                                                                                                               <  1:774646/150‑1 (MQ=255)
        aaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGc                                                                                                                                              <  1:3714976/150‑1 (MQ=255)
        aaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGc                                                                                                                                              <  2:2770873/150‑1 (MQ=255)
         aaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑Atttttttttttttttcc                                                                                                                                              >  2:4492455/1‑145 (MQ=255)
         aaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGcc                                                                                                                                             <  2:3739124/150‑1 (MQ=255)
           aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGTGTTC‑TGtttttttttttatttaac                                                                                                                                            >  1:4578166/1‑143 (MQ=255)
           aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑Attttttttttttttttcat                                                                                                                                            >  1:2070197/1‑143 (MQ=255)
             gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTTTTTTTT‑TTCCCTga                                                                                                                                         >  1:4491962/1‑150 (MQ=255)
             gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATga                                                                                                                                         <  2:2289489/150‑1 (MQ=255)
              cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTT‑TTCCATgag                                                                                                                                        >  1:1343058/1‑150 (MQ=255)
               gTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTT‑ATTTTTTTTTTTTT‑TTCCATgata                                                                                                                                       >  2:4140242/1‑148 (MQ=255)
               gTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTTTTTTTT‑TTCCCTgaga                                                                                                                                       >  2:3904848/1‑150 (MQ=255)
               gTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATgaga                                                                                                                                       <  1:2004870/150‑1 (MQ=255)
                tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTTTTTTTT‑TTCCCTTAGaa                                                                                                                                      >  2:1615741/1‑150 (MQ=255)
                tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGaa                                                                                                                                      <  2:1504183/150‑1 (MQ=255)
                  aTGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTTTTTTTT‑TTCCCTGAGAAgg                                                                                                                                    >  2:1290559/1‑149 (MQ=255)
                  aTGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGt                                                                                                                                    <  2:3213904/150‑1 (MQ=255)
                   tGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑CTTTTTTTTTTTTT‑TTTCCTGAGAAGTg                                                                                                                                   >  1:3098091/1‑150 (MQ=255)
                   tGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑CTTTTTTTTTTTTT‑TTCCCTTCGAAGTg                                                                                                                                   >  1:3238613/1‑150 (MQ=255)
                     agtagtATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTT‑TTCCATGAGTAGTGAg                                                                                                                                 >  1:2662158/1‑150 (MQ=255)
                     agtagtATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAg                                                                                                                                 <  1:6930/150‑1 (MQ=255)
                       tagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTT‑ATTTTTTTTTTTTT‑TTCCCTTAGAAAAGAgcg                                                                                                                               >  2:1975226/1‑150 (MQ=255)
                             cGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCagaga                                                                                                                         <  1:1368447/150‑1 (MQ=255)
                              ggTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTTTTTTTT‑TTCCCTGGGAAAGGAGGGCagagag                                                                                                                        >  2:3106984/1‑150 (MQ=255)
                               gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAgg                                                                                                                       <  1:2242540/150‑1 (MQ=255)
                                tATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                                      <  2:1113081/150‑1 (MQ=255)
                                   tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCTGTTTTTTTTTTTTT‑TGCC‑TGTGGAGGGGGGGCAGAGAgggggt                                                                                                                   >  2:2503334/1‑147 (MQ=255)
                                   tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGAtt                                                                                                                   <  1:3522186/150‑1 (MQ=255)
                                     agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTTTTTTTT‑TTCCATGAGAAGGGAGCGCAGAGAGGGGTTAt                                                                                                                 >  2:3601994/1‑150 (MQ=255)
                                     agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTAt                                                                                                                 <  1:2490870/150‑1 (MQ=255)
                                         tttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑CGTTTTTTTTTTTT‑TTTCCTGAGAAGTGAGCGCCGAGAGGGGGTTTCTTg                                                                                                             >  2:2437276/1‑150 (MQ=255)
                                         tttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTA‑ATTTTTTTTTTTTT‑TTCCATGAGAAGAGAGAGGAGAGCGGGATTAttttt                                                                                                             >  2:2661109/1‑149 (MQ=255)
                                           tATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTTTTTTTT‑TTCCCTTAGAAGAGAGCGCAGAGAGGGGTTTTCTTgtc                                                                                                           >  2:3956771/1‑148 (MQ=255)
                                                aGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctgact                                                                                                      <  1:1437583/150‑1 (MQ=255)
                                                aGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATAAGAAGAGAGGGCGGGGAGGGAATATCTTTCccgact                                                                                                      >  1:302505/1‑150 (MQ=255)
                                                  aaaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTTTTTTTT‑TTCCCTTAGAAGTGGGCGCAGAGAGGGGTTATTTTTGctgactta                                                                                                    >  2:1244643/1‑148 (MQ=255)
                                                    aGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTT‑CTTTTTTGTTTTTCTTTCCTTGA‑AAGGGATAGCGGGGAGGGATTCTCTTTCctgaatgtaa                                                                                                  >  1:3861292/1‑147 (MQ=255)
                                                     gTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑TTTTTTTTTTTTTT‑TTCCATGTGAAGGGGGGGCAGAGAGGGGTTTTCTTTCTTGTCTGaatt                                                                                                 >  2:1845502/1‑148 (MQ=255)
                                                      tttGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTTTTTTTT‑TTCCCTGCGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTGACTGAACTg                                                                                                >  2:4383675/1‑150 (MQ=255)
                                                        tGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGc                                                                                              <  2:4187437/150‑1 (MQ=255)
                                                           gggTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑TTTTTTTTTTTTTT‑TGCCCTGAGGAGGGAGAGGAGAGAGGGGTTATCTTTCCTGACTGAACTGGCGac                                                                                           >  2:1802272/1‑149 (MQ=255)
                                                            ggTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGAtt                                                                                          <  1:3732754/150‑1 (MQ=255)
                                                               aaCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAg                                                                                       <  1:3354530/150‑1 (MQ=255)
                                                                  ccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGttt                                                                                    <  2:2140995/150‑1 (MQ=255)
                                                                   cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑CTTTTTTTTTTTTT‑TGTCTTGAGGAGAGAGCGCAGGGAGGGAGTTTTTTGCCTGGCTGAAATGGCGGTTAAGtttt                                                                                   >  2:1879051/1‑149 (MQ=255)
                                                                    ttATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTTTTTTTT‑TTCCCTGAGAAGTGAGCGCAGGGAGGGGGTATCTTTCCTGACTGAAATGGGGATTTAAttttc                                                                                  >  2:3973292/1‑148 (MQ=255)
                                                                             ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGC‑ATTTTTTTTTTTTT‑TTCCCTGAGGAGAGAGCGCAGAGAGGGAGTATCTTTTCTTACTTAAATGGCGGTTAAGTTTTCCCGTAacct                                                                         >  2:3511350/1‑149 (MQ=255)
                                                                                 aCCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGCC‑GTTTTTTTTTTTTT‑TGCCATTGGAAGAGAGGGCAGGGAGGGGTTTTCTTGTCTCACTGGACTGGCGATTTAAGTTCCGCGTACACcgcgg                                                                     >  2:731215/1‑149 (MQ=255)
                                                                                  ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGtt                                                                    <  1:3987244/150‑1 (MQ=255)
                                                                                   cTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGC‑ATTTTTTTTTTTTT‑TTCCATGAGAAGTGAGCGCCGAGGGGGGGTATCTTTCCTGACTGAACTGGCGGTTAAGTTTTCGCGTACCCCGCGgtt                                                                   >  2:2001200/1‑150 (MQ=255)
                                                                                    tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGC‑ATTTTTTTTTTTTT‑TTCCCTGAGAAGAGAGGGGAGAGAGGGGGTTTTTTTTCTGACTGTACTGGCGGTTTAATTTTCGCGTACCCGGCGtttt                                                                  >  2:224410/1‑149 (MQ=255)
                                                                                     ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAg                                                                 <  2:1155978/150‑1 (MQ=255)
                                                                                     ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAg                                                                 <  1:2729779/150‑1 (MQ=255)
                                                                                          aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTAtt                                                            <  2:3707992/150‑1 (MQ=255)
                                                                                            tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGc                                                          <  1:644968/150‑1 (MQ=255)
                                                                                            tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGc                                                          <  1:2860235/150‑1 (MQ=255)
                                                                                              aGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCgg                                                        <  1:586207/150‑1 (MQ=255)
                                                                                              aGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCgg                                                        <  1:2320530/150‑1 (MQ=255)
                                                                                               ggAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGga                                                       <  1:4238989/150‑1 (MQ=255)
                                                                                                gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgat                                                      <  2:912920/150‑1 (MQ=255)
                                                                                                 aGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatg                                                     <  1:4332448/150‑1 (MQ=255)
                                                                                                   tCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgat                                                   <  2:1015842/150‑1 (MQ=255)
                                                                                                   tCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGAC‑ATTTTTTTTTTTTT‑TTCCATGGGTAGTGAGCGCCGGGAGGGGTTGTCTTTTCTGTCTGAACATGCGGTTTAATTTCCCCGTAACCGGCGTTTTGTTATTTTGggtgat                                                   >  2:4420141/1‑150 (MQ=255)
                                                                                                     aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGC‑ATTTTTTTTTTTTT‑TTCCCTGAGAAGAGAGGGGAGGGGGGGGGTATTTTGGCTGACTGGACTGGGGGTTAAGTTTTCGCGTACCCCGCGGTTTGTTTTTGTggggggtga                                                 >  2:943938/1‑143 (MQ=255)
                                                                                                        tCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTTTTTTTT‑TTTCCTGAGGAGGGAGGGGCGGGAGGGGGTATTTTTCCCGACTGAACTGGCGGTTAAGGTTCCCCGGACCCCGCGGTTTGTTTTTTTGGGTGGTGatat                                              >  2:4247078/1‑150 (MQ=255)
                                                                                                          aGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTTTTTTTT‑TTCCCTGAGAAGGGAGGGGAGGGAGGGGGTATTTTTCCTTACTGAACTGGCGATTAAGTTTCCGCGTACCCCGCGGTTTGTTTTTGCGGGTGATGTTAttt                                            >  2:786324/1‑149 (MQ=255)
                                                                                                              tGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGc                                        <  2:1192403/150‑1 (MQ=255)
                                                                                                               gCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCt                                       <  1:1384337/150‑1 (MQ=255)
                                                                                                                 cATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCg                                     <  2:3165273/150‑1 (MQ=255)
                                                                                                                        cgGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGAtt                              <  1:509527/150‑1 (MQ=255)
                                                                                                                          gATCTCGTTTGCGGCGGTC‑ATTTTTTTTTTTTT‑TTCCATGAGAAGGGAGCGGGGGGGGGGGTTATTTTTCCTGACTTAACTGGGGATTAAATTTTCGCGTACCCGGGGGTTTTTTTTTGTGGGGGATTGTATTTAAGGTCGGCTGGTTgg                            >  2:2870775/1‑150 (MQ=255)
                                                                                                                           aTCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGt                           <  1:4033579/150‑1 (MQ=255)
                                                                                                                               cGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAt                       <  1:1827955/150‑1 (MQ=255)
                                                                                                                                gTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAtt                      <  2:2587751/150‑1 (MQ=255)
                                                                                                                                gTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAtt                      <  2:4251771/150‑1 (MQ=255)
                                                                                                                                gTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAtt                      <  1:2573466/150‑1 (MQ=255)
                                                                                                                                    gcggcgGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCCCGGCTGATTGGTTCATTGTCg                  <  1:2682268/150‑1 (MQ=255)
                                                                                                                                          gTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCa            >  1:1002206/1‑150 (MQ=255)
                                                                                                                                          gTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCa            <  1:2391871/150‑1 (MQ=255)
                                                                                                                                          gTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCa            >  2:1690524/1‑150 (MQ=255)
                                                                                                                                          gTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCa            >  2:2034882/1‑150 (MQ=255)
                                                                                                                                            c‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAcc          >  1:3908573/1‑150 (MQ=255)
                                                                                                                                            c‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAcc          >  2:2818601/1‑150 (MQ=255)
                                                                                                                                               ttttttCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTGTCTTGCCTGACTGAACTGGCGATTAAGGTTCCGCGGACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGc        >  2:3307605/1‑150 (MQ=255)
                                                                                                                                               ttttttCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGc        <  1:3174566/150‑1 (MQ=255)
                                                                                                                                                tttttctttttc‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCt       >  2:158908/1‑150 (MQ=255)
                                                                                                                                                tttttctttttc‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCt       >  1:1066240/1‑150 (MQ=255)
                                                                                                                                                 ttttctttttc‑tGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTg      >  1:444344/1‑150 (MQ=255)
                                                                                                                                                 ttttctttttc‑tGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTg      >  2:1752889/1‑150 (MQ=255)
                                                                                                                                                   ttctttttc‑tGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGa    >  2:3462494/1‑150 (MQ=255)
                                                                                                                                                   ttctttttc‑tGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGa    >  1:1706090/1‑150 (MQ=255)
                                                                                                                                                    tctttttc‑tGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc   <  1:1457958/150‑1 (MQ=255)
                                                                                                                                                    tctttttc‑tGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc   <  2:6361/150‑1 (MQ=255)
                                                                                                                                                    tctttttc‑tGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc   >  2:2553403/1‑150 (MQ=255)
                                                                                                                                                    tcttttcc‑tGCCATGAGAAGTGAGCGCAGAGAGGGATTTTCTTGCCTGACTGAACGGGCGATTAAGTTTCCGCGTCCCCGGCGTTTATTTATGGGGGAGGATGATATTGAAGCTCGGCTGTTTGTTTCATTGCCGCCACCCCCGCTGGAc   <  2:2953111/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTC‑ATTTTTTCTTTTTC‑TGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACG  >  AP012306/3938739‑3939036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑