Predicted mutation
evidence seq id position mutation annotation gene description
RA AP012306 574,429 G→C Y218* (TAC→TAG)  nagA ← N‑acetylglucosamine‑6‑phosphate deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP012306574,4290GC99.7% 1580.1 / ‑4.7 387Y218* (TAC→TAG) nagAN‑acetylglucosamine‑6‑phosphate deacetylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (225/161);  minor base T (0/1);  total (225/162)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.65e-01

Only 100 of 396 total aligned reads displayed.

TTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGT  >  AP012306/574282‑574575
                                                                                                                                                   |                                                                                                                                                  
tttGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTa                                                                                                                                                  >  1:1300126/1‑150 (MQ=255)
 ttGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTAc                                                                                                                                                 <  1:2141799/150‑1 (MQ=255)
     cGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGAt                                                                                                                                             >  2:2611200/1‑150 (MQ=255)
     cGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGAt                                                                                                                                             >  2:3759033/1‑150 (MQ=255)
      gCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATg                                                                                                                                            >  2:331671/1‑150 (MQ=255)
       ccTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATgg                                                                                                                                           >  1:3857403/1‑150 (MQ=255)
        cTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATggg                                                                                                                                          >  2:4320317/1‑150 (MQ=255)
         tttCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGt                                                                                                                                         >  1:2248090/1‑150 (MQ=255)
          ttCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTg                                                                                                                                        >  1:1959712/1‑150 (MQ=255)
           tCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTgg                                                                                                                                       <  2:3887775/150‑1 (MQ=255)
            cAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGc                                                                                                                                      <  1:718641/150‑1 (MQ=255)
             aGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACCTGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCa                                                                                                                                     >  2:860253/1‑150 (MQ=255)
                     aGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAAt                                                                                                                             >  2:2320534/1‑150 (MQ=255)
                     aGCGTTGCGAATGTTGGCATAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAAt                                                                                                                             <  1:300563/150‑1 (MQ=255)
                        gTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTAGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATccc                                                                                                                          <  1:2869929/150‑1 (MQ=255)
                         ttGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATcccc                                                                                                                         >  2:3980298/1‑150 (MQ=255)
                          tGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCcg                                                                                                                        <  2:698401/150‑1 (MQ=255)
                           gCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCcgc                                                                                                                       >  2:1770895/1‑150 (MQ=255)
                             gAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCcgcgc                                                                                                                     >  1:4044109/1‑150 (MQ=255)
                             gAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCcgcgc                                                                                                                     >  2:1352171/1‑150 (MQ=255)
                                 gTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGaa                                                                                                                 <  2:1232746/150‑1 (MQ=255)
                                   tGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAAc                                                                                                               <  1:802495/150‑1 (MQ=255)
                                        tAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCt                                                                                                          <  2:3583687/150‑1 (MQ=255)
                                        tAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCt                                                                                                          >  1:3273347/1‑150 (MQ=255)
                                         aaTCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCtt                                                                                                         >  1:3200372/1‑150 (MQ=255)
                                         aaTCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCtt                                                                                                         <  2:1676335/150‑1 (MQ=255)
                                           tCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCtttt                                                                                                       >  1:4516907/1‑150 (MQ=255)
                                             aaCATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGc                                                                                                     >  2:1567923/1‑150 (MQ=255)
                                             aaCATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGc                                                                                                     >  1:64480/1‑150 (MQ=255)
                                               cATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGctt                                                                                                   >  2:1941797/1‑150 (MQ=255)
                                                   cAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTGACGACCGGTAATATACGGCATCGCGTTCTACAAATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCttt                                                                                               >  2:3375691/1‑150 (MQ=255)
                                                      gCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCaa                                                                                            <  1:2972100/150‑1 (MQ=255)
                                                      gCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCaa                                                                                            <  2:1310972/150‑1 (MQ=255)
                                                       ccATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAAc                                                                                           >  2:307646/1‑150 (MQ=255)
                                                        cATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACg                                                                                          <  1:1613133/150‑1 (MQ=255)
                                                            aGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTcgc                                                                                      <  2:2731473/150‑1 (MQ=255)
                                                              cAATAATACCGCAATAAATGACAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGt                                                                                    >  2:4384129/1‑150 (MQ=255)
                                                                 taataCCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTgg                                                                                 <  1:1223078/150‑1 (MQ=255)
                                                                 taataCCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTgg                                                                                 >  2:1617402/1‑150 (MQ=255)
                                                                 taataCCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGACAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTgg                                                                                 <  2:4503559/150‑1 (MQ=255)
                                                                  aataCCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGa                                                                                >  1:3665468/1‑150 (MQ=255)
                                                                     aCCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTg                                                                             >  2:2773274/1‑150 (MQ=255)
                                                                         cAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCg                                                                         <  2:4026683/150‑1 (MQ=255)
                                                                         cAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCg                                                                         <  2:526906/150‑1 (MQ=255)
                                                                          aaTAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCgg                                                                        <  2:3354427/150‑1 (MQ=255)
                                                                             aaaTGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAg                                                                     >  2:3853828/1‑150 (MQ=255)
                                                                             aaaTGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAg                                                                     <  1:1538980/150‑1 (MQ=255)
                                                                               aTGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGaa                                                                   >  1:166279/1‑150 (MQ=255)
                                                                                tGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGaaa                                                                  <  2:3234769/150‑1 (MQ=255)
                                                                                  tCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAAcc                                                                >  1:4402003/1‑150 (MQ=255)
                                                                                    aGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACcac                                                              >  2:2252374/1‑150 (MQ=255)
                                                                                       ttCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAAt                                                           <  1:4109058/150‑1 (MQ=255)
                                                                                       ttCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAAt                                                           >  1:3384550/1‑150 (MQ=255)
                                                                                        tcgtcgAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGAGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATc                                                          <  1:4273711/150‑1 (MQ=255)
                                                                                         cgtcgAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATcc                                                         >  1:2413310/1‑150 (MQ=255)
                                                                                          gtcgAGGGTCGCGCCCCCCCGGCCCGGGTCACGACCGGGAAAATACGGGATCGCGGTCTACAGATGGGGGGCCAAGGGAATCCCCCCGCGGGAACCCGCCTTTGCTTCTTTCAACGTCGCGGTGGGGGGACCGGCAGAAAACACAAAccc                                                        >  2:4144230/1‑150 (MQ=255)
                                                                                          gtcgAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATccc                                                        >  2:3628410/1‑150 (MQ=255)
                                                                                          gtcgAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATccc                                                        <  1:1048599/150‑1 (MQ=255)
                                                                                            cgAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCgg                                                      <  2:2152973/150‑1 (MQ=255)
                                                                                             gAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGc                                                     >  2:650711/1‑150 (MQ=255)
                                                                                               ggATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCAt                                                   <  1:4218135/150‑1 (MQ=255)
                                                                                               ggATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCAt                                                   >  2:2999546/1‑150 (MQ=255)
                                                                                                 aTCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGACACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCAttt                                                 >  1:3046274/1‑150 (MQ=255)
                                                                                                 aTCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCAttt                                                 <  2:3493376/150‑1 (MQ=255)
                                                                                                    gcgcCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGcc                                              <  1:2641136/150‑1 (MQ=255)
                                                                                                       cccgccAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGt                                           >  1:1471158/1‑150 (MQ=255)
                                                                                                          gccaggccagGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTg                                        <  2:1057018/150‑1 (MQ=255)
                                                                                                          gccaggccagGTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTg                                        >  1:2803961/1‑150 (MQ=255)
                                                                                                           ccaggccaggTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGc                                       <  1:2736591/150‑1 (MQ=255)
                                                                                                           ccaggccaggTGCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGc                                       >  1:3987111/1‑150 (MQ=255)
                                                                                                            caggccaggTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCt                                      >  1:2340862/1‑150 (MQ=255)
                                                                                                              ggccaggTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCtga                                    <  2:2970309/150‑1 (MQ=255)
                                                                                                              ggccaggTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCtga                                    <  1:1105916/150‑1 (MQ=255)
                                                                                                               gccaggTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCtgat                                   <  2:1287606/150‑1 (MQ=255)
                                                                                                               gccaggTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCtgat                                   >  1:255810/1‑150 (MQ=255)
                                                                                                                ccaggTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCtgatg                                  >  1:858602/1‑150 (MQ=255)
                                                                                                                ccaggTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCtgatg                                  >  1:2062153/1‑150 (MQ=255)
                                                                                                                ccaggTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCtgatg                                  <  2:814483/150‑1 (MQ=255)
                                                                                                                ccaggTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCtgatg                                  >  1:4305720/1‑150 (MQ=255)
                                                                                                                 caggTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCtgatga                                 <  2:2873468/150‑1 (MQ=255)
                                                                                                                  aggTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGAc                                >  1:961041/1‑150 (MQ=255)
                                                                                                                  aggTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGAc                                >  2:2444389/1‑150 (MQ=255)
                                                                                                                  aggTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGAc                                >  1:1783585/1‑150 (MQ=255)
                                                                                                                   ggTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACt                               >  2:1138896/1‑150 (MQ=255)
                                                                                                                    gTTCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACtt                              >  1:2007759/1‑150 (MQ=255)
                                                                                                                     ttCACGACCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTc                             <  1:3607271/150‑1 (MQ=255)
                                                                                                                           aCCGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAgg                       >  1:3534178/1‑150 (MQ=255)
                                                                                                                             cGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGaa                     >  1:475485/1‑150 (MQ=255)
                                                                                                                             cGGTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGaa                     <  1:4461127/150‑1 (MQ=255)
                                                                                                                              ggTAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAAc                    <  2:3173291/150‑1 (MQ=255)
                                                                                                                                tAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCa                  >  1:1884876/1‑150 (MQ=255)
                                                                                                                                tAATATACGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCa                  >  1:2916557/1‑150 (MQ=255)
                                                                                                                                   tataCGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCAttt               <  1:3763680/150‑1 (MQ=255)
                                                                                                                                    ataCGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTc              >  2:902311/1‑150 (MQ=255)
                                                                                                                                    ataCGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTc              >  2:2026169/1‑150 (MQ=255)
                                                                                                                                      aCGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCg            >  1:2095203/1‑150 (MQ=255)
                                                                                                                                      aCGGCATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCg            >  2:2562415/1‑150 (MQ=255)
                                                                                                                                          cATCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGc        >  2:2368963/1‑150 (MQ=255)
                                                                                                                                            tCGCGTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCa      >  2:2099872/1‑150 (MQ=255)
                                                                                                                                               cgTTCTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAggg   <  1:1015298/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGT  >  AP012306/574282‑574575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑