BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AP012306	1,587,473	G→A	Q37* (CAG→TAG)	cspC ←	stress protein, member of the CspA‑family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AP012306	1,587,473	0	G	A	100.0%	1716.9 / NA	414	Q37* (CAG→TAG)	cspC	stress protein, member of the CspA‑family
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (229/185); total (229/185)

Only 100 of 424 total aligned reads displayed.

TATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGC  >  AP012306/1587326‑1587619

 ataGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGAt                                                                                                                                                  <  1:2503362/150‑1 (MQ=255)

 ataGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGAt                                                                                                                                                  >  2:419258/1‑150 (MQ=255)

     cGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCg                                                                                                                                              >  2:727999/1‑150 (MQ=255)

      gagaTTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCgg                                                                                                                                             >  2:1374887/1‑150 (MQ=255)

      gagaTTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCgg                                                                                                                                             <  2:3755589/150‑1 (MQ=255)

       agaTTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGga                                                                                                                                            >  1:4250594/1‑150 (MQ=255)

           tGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAg                                                                                                                                       >  2:1896351/1‑150 (MQ=255)

             aaGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAgtg                                                                                                                                     <  1:2638127/150‑1 (MQ=255)

              aGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAgtgt                                                                                                                                    >  1:1663470/1‑150 (MQ=255)

              aGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAgtgt                                                                                                                                    >  1:3897393/1‑150 (MQ=255)

               gCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTa                                                                                                                                   >  2:3492182/1‑150 (MQ=255)

                cGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTAc                                                                                                                                  <  1:3637535/150‑1 (MQ=255)

                 gTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACg                                                                                                                                 >  1:3490510/1‑150 (MQ=255)

                  tATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGa                                                                                                                                >  1:1898192/1‑150 (MQ=255)

                  tATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGCTTTGAAGCCATTACCCTAGATAGCGTAG‑AAGTGTACGa                                                                                                                                >  1:3313146/1‑150 (MQ=255)

                    ttCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAac                                                                                                                              <  1:2547868/150‑1 (MQ=255)

                    ttCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAac                                                                                                                              <  2:1594182/150‑1 (MQ=255)

                    ttCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAac                                                                                                                              >  2:553087/1‑150 (MQ=255)

                      cacaCTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAacac                                                                                                                            >  1:2021580/1‑150 (MQ=255)

                       acacTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAAcaca                                                                                                                           >  1:4350888/1‑150 (MQ=255)

                         acTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATc                                                                                                                         <  1:1366723/150‑1 (MQ=255)

                         acTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATc                                                                                                                         >  1:655788/1‑150 (MQ=255)

                          cTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCt                                                                                                                        <  1:619026/150‑1 (MQ=255)

                            tCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCttt                                                                                                                      <  2:4084106/150‑1 (MQ=255)

                               gATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTtgct                                                                                                                   <  2:3690611/150‑1 (MQ=255)

                               gATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTtgct                                                                                                                   <  2:4534689/150‑1 (MQ=255)

                                    gTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCAt                                                                                                              <  1:1593237/150‑1 (MQ=255)

                                      ggATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCa                                                                                                            <  2:2373467/150‑1 (MQ=255)

                                      ggATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCa                                                                                                            <  2:1088148/150‑1 (MQ=255)

                                       gATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAg                                                                                                           <  2:131612/150‑1 (MQ=255)

                                         ttCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAG‑TTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCg                                                                                                        >  1:1138732/1‑150 (MQ=255)

                                           cGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCgg                                                                                                       >  1:2763626/1‑150 (MQ=255)

                                            gATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGa                                                                                                      >  2:875340/1‑150 (MQ=255)

                                            gATCAGATAGCTGCTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGATTTGAAGCCATTACCCTAGATAGCCGAG‑AAGCGCACGACCACATCTTTGCTGCCATCAGCCGGa                                                                                                      >  2:1489806/1‑150 (MQ=255)

                                             aTCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAg                                                                                                     <  2:1447930/150‑1 (MQ=255)

                                             aTCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAg                                                                                                     <  1:1267468/150‑1 (MQ=255)

                                                  aTAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATg                                                                                                >  2:4502621/1‑150 (MQ=255)

                                                   tAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGa                                                                                               >  1:4168192/1‑150 (MQ=255)

                                                   tAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGa                                                                                               <  2:245939/150‑1 (MQ=255)

                                                       tGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGcc                                                                                           >  1:1728097/1‑150 (MQ=255)

                                                       tGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGcc                                                                                           >  2:2949936/1‑150 (MQ=255)

                                                       tGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGcc                                                                                           >  2:2545448/1‑150 (MQ=255)

                                                         ttACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCaa                                                                                         >  2:923850/1‑150 (MQ=255)

                                                            cGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc                                                                                      >  1:1161001/1‑150 (MQ=255)

                                                             gTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAcc                                                                                     >  1:99823/1‑150 (MQ=255)

                                                              ttAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCt                                                                                    >  1:2405139/1‑150 (MQ=255)

                                                              ttAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCt                                                                                    >  2:1657716/1‑150 (MQ=255)

                                                               tAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCtt                                                                                   >  1:1848374/1‑150 (MQ=255)

                                                                  cAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAg                                                                                <  1:1507721/150‑1 (MQ=255)

                                                                  cAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAg                                                                                <  1:4487332/150‑1 (MQ=255)

                                                                   aGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGa                                                                               >  1:2861350/1‑150 (MQ=255)

                                                                   aGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGa                                                                               >  2:1715717/1‑150 (MQ=255)

                                                                      tGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTc                                                                            >  1:3110713/1‑150 (MQ=255)

                                                                       gCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCg                                                                           <  2:1161001/150‑1 (MQ=255)

                                                                        ccGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGt                                                                          <  2:4237058/150‑1 (MQ=255)

                                                                         cGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGtt                                                                         >  2:3216700/1‑150 (MQ=255)

                                                                          ggACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTg                                                                        >  2:2994467/1‑150 (MQ=255)

                                                                              cTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAAcc                                                                    >  1:3715236/1‑150 (MQ=255)

                                                                                ttCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCAc                                                                  >  1:2998154/1‑150 (MQ=255)

                                                                                  cTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAg                                                                                                           >  2:2226719/1‑107 (MQ=255)

                                                                                   tGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTa                                                               <  2:21416/150‑1 (MQ=255)

                                                                                     gCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAAc                                                             <  1:3396913/150‑1 (MQ=255)

                                                                                       cGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCt                                                           >  1:2230495/1‑150 (MQ=255)

                                                                                         tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGa                                                         >  2:415938/1‑150 (MQ=255)

                                                                                          ccTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGAc                                                        >  1:317415/1‑150 (MQ=255)

                                                                                           cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGAcc                                                       <  2:4411020/150‑1 (MQ=255)

                                                                                            tGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑TAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGAAATGAAGCCAAAACATTTAGACTCGTTGAACCATTTAACCTGACCt                                                      >  2:2788319/1‑150 (MQ=255)

                                                                                             gAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCtt                                                     <  2:1750540/150‑1 (MQ=255)

                                                                                              aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCttt                                                    >  1:947905/1‑150 (MQ=255)

                                                                                                tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTaa                                                  >  1:4089654/1‑150 (MQ=255)

                                                                                                   cGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCt                                               <  2:3551646/150‑1 (MQ=255)

                                                                                                       cTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGc                                           <  1:1018589/150‑1 (MQ=255)

                                                                                                       cTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGc                                           >  2:257762/1‑150 (MQ=255)

                                                                                                        tCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGcc                                          <  1:198752/150‑1 (MQ=255)

                                                                                                          aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt                                        >  2:3982350/1‑150 (MQ=255)

                                                                                                          aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt                                        <  1:2124296/150‑1 (MQ=255)

                                                                                                              ttCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGa                                    <  1:471271/150‑1 (MQ=255)

                                                                                                               tCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaa                                   >  1:3570295/1‑150 (MQ=255)

                                                                                                               tCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaa                                   <  1:368870/150‑1 (MQ=255)

                                                                                                                   aCCTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAAt                               <  2:3244859/150‑1 (MQ=255)

                                                                                                                    ccTTCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAAtt                              <  1:3789747/150‑1 (MQ=255)

                                                                                                                      ttCAGCCAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTcc                            <  2:339174/150‑1 (MQ=255)

                                                                                                                           ccAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGa                       >  1:858667/1‑150 (MQ=255)

                                                                                                                           ccAGAGTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGa                       <  1:4538243/150‑1 (MQ=255)

                                                                                                                             agagTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGAtt                     <  2:661207/150‑1 (MQ=255)

                                                                                                                              gagTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTg                    >  1:3229529/1‑150 (MQ=255)

                                                                                                                               agTTTTGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGt                   >  1:4015000/1‑150 (MQ=255)

                                                                                                                                  tttGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGtttt                >  2:2884303/1‑150 (MQ=255)

                                                                                                                                  tttGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGtttt                >  1:3936489/1‑150 (MQ=255)

                                                                                                                                   ttGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTttc               <  1:2348375/150‑1 (MQ=255)

                                                                                                                                    tGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTttct              <  1:553833/150‑1 (MQ=255)

                                                                                                                                    tGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTttct              <  2:307211/150‑1 (MQ=255)

                                                                                                                                    tGAAGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTttct              <  2:1795530/150‑1 (MQ=255)

                                                                                                                                       aGCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCg           <  2:1770947/150‑1 (MQ=255)

                                                                                                                                        gCCATTACCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGc          >  2:3826436/1‑150 (MQ=255)

                                                                                                                                           aTTACCCTGAAAAG‑GGAGAAAGGTTACGAACCCTTCTTTGTGCCCATCGGCCGGAGT‑ATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGc      <  2:2665357/150‑1 (MQ=255)

                                                                                                                                              aCCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAg    >  2:3917788/1‑150 (MQ=255)

                                                                                                                                              aCCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAg    >  2:3029195/1‑150 (MQ=255)

                                                                                                                                              aCCCTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAg    >  2:2045634/1‑150 (MQ=255)

                                                                                                                                                ccTAGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGc  >  1:736554/1‑150 (MQ=255)

TATAGCGAGATTGAAGCGTATTCACACTTCAGATCAGTGGATTCGATCAGATAGCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAG‑AAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGC  >  AP012306/1587326‑1587619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`