New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_012967 3462691 =127 (0.670)3 (0.020) 3/136 14.2 1.9% coding (1190/1623 nt) pckA phosphoenolpyruvate carboxykinase (ATP)
?NC_012967 3462751 = 189 (1.050)coding (1250/1623 nt) pckA phosphoenolpyruvate carboxykinase (ATP)
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 128 total aligned reads displayed.

CCTGACTGCCGATCAAACCCAGTATCACTTCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCTGTCGCTGCACCCGACTCAG  >  NC_012967/3462620‑3462761
                                                                         |                                                                      
ccTGACTGCCGATCAAACCCAGTATCACTTCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑gg                                                                        <  1:6409548/72‑1 (MQ=255)
ccTGACTGCCGATCAAACCCAGTATCACTTCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACAGTGCgg‑gg                                                                        >  1:3096699/1‑72 (MQ=255)
 cTGACTGCCGATCAAACCCAGTATCACTTCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGc                                                                       >  2:3257842/1‑72 (MQ=255)
  tGACTGCCGATCAAACCCAGTATCACTTCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCa                                                                      <  1:1115345/72‑1 (MQ=255)
   gACGGCCGATCAAACCCAGTATCACTTCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCAt                                                                     <  2:3996121/72‑1 (MQ=255)
    ccTGCCGATCAAACCCAGTATCACTTCCTCTCTGGCTTCACCGCCAAANTGGCCGG‑TACTGAGCGT‑GGCATc                                                                    <  1:4264405/71‑1 (MQ=255)
    aCTGCCGATCAAACCCAGTATCACTTCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATc                                                                    <  1:1470526/72‑1 (MQ=255)
    aCTGCCGATCAAACCCAGTATCACTTCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATc                                                                    >  1:4610280/1‑72 (MQ=255)
    aCTGCCGATCAAACCCAGTATCACTTCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCCT‑GGCAtt                                                                    >  2:1621301/1‑71 (MQ=255)
    aCTGCCGATCAAACCCAGTATCACTTCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑GACTGAGCGT‑GGCacc                                                                    >  1:4612900/1‑70 (MQ=255)
     cTGCCGATCAAACCCAGTATCACTTCCTCTCTGGCTTCACCGCCAAACTGGCCGGCCCCCGAGCGT‑GGCacc                                                                    >  1:5788525/1‑70 (MQ=255)
     cTGCCGATCAAACCCAGTATCACTTCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCa                                                                   <  1:6437332/72‑1 (MQ=255)
      tGCCGATCAAACCCAGTATCACTTCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCac                                                                  <  2:6294911/72‑1 (MQ=255)
      tGCCGATCAAACCCAGTAGCACTTCCTCTCTGGCGTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCac                                                                  <  2:2745693/72‑1 (MQ=255)
       tccGATCAAACCCATTATCACTTCCTCTCTGCCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCacc                                                                 <  1:1812021/71‑1 (MQ=255)
       gCCGATCAAACCCAGTATCACTTCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCacc                                                                 <  2:6634158/72‑1 (MQ=255)
       gCCGATCAAACCCAGTATCACTTCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCacc                                                                 <  1:6247447/72‑1 (MQ=255)
         cGATCAACCCCAGTACCACTTCCTCTCGGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCaccga                                                               <  1:2380402/72‑1 (MQ=255)
         cGATCAAACCCAGTATCACTTCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCaccga                                                               <  1:1504822/72‑1 (MQ=255)
          gATCAAACCCAGTATCACTTCCTCTCTGGCTTCACCGCCCAACTGGCCGG‑TTCTTAGCGTGGGCTTCaccgc                                                               >  1:1081803/1‑71 (MQ=255)
          gATCAAACCCAGTATCACTTCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCaccgaa                                                              <  1:6533510/72‑1 (MQ=255)
           aTCAAACCCACAAACACTTCCTCTCGGGCTCCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCaccgaac                                                             <  2:3533897/72‑1 (MQ=255)
            tCAACCCCAGTATCCCTTCCTCTCTGGCGTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCaccgaacc                                                            <  2:6408401/72‑1 (MQ=255)
             cAACCCCGGTACCACGCCCTCTCTGGCTTCCCCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCaccgaaccg                                                           <  1:2606156/72‑1 (MQ=255)
             cAAACCCAGTCCCCCTCCCTCCCTGCCTCCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCaccgaaccg                                                           <  1:1584140/72‑1 (MQ=255)
              aaaCCCAGTATCACTTCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGGATCaccggaccca                                                          >  2:3319288/1‑70 (MQ=255)
              aaaCCCAGTATCACTTCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCaccgaaccga                                                          <  1:4676775/72‑1 (MQ=255)
              aaaCCCAGTATCACTTCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCaccgaaccga                                                          >  2:5250816/1‑72 (MQ=255)
                ccccATTACCCCTCCCTCTCGGGCTCCCCCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCACCGAACCGACg                                                        <  2:5570510/71‑1 (MQ=255)
                 cccAGTCCCACTCCCTCTCGGGCTCCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCACCGAACCGACGc                                                       <  2:383643/72‑1 (MQ=255)
                   ctgTAGCACTTCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCACCGAACCGACGCCa                                                     <  1:3194025/70‑1 (MQ=255)
                    aGTATCCCTTCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGGATCAACGTACCCACGACaa                                                    >  2:2587790/1‑72 (MQ=255)
                     gTATCACTTCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAAc                                                   <  1:2705451/72‑1 (MQ=255)
                      tATCCCTTCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAAcc                                                  <  1:5311698/72‑1 (MQ=255)
                      tATCACTTCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCACCGAACAGACGCCAAcc                                                  <  1:5371255/72‑1 (MQ=255)
                       accACTTCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCt                                                 <  1:1291073/70‑1 (MQ=255)
                       aTCACTTCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCt                                                 <  1:4739597/72‑1 (MQ=255)
                       aTCACTCCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCt                                                 <  2:4024765/72‑1 (MQ=255)
                         cACTTCCTCTCTGGCTTCACCTCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCACCGAACCGACGGCAACCTtc                                               >  1:4851604/1‑72 (MQ=255)
                          aCTTCCTCCCGGGCTCCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTtct                                              <  1:1458240/72‑1 (MQ=255)
                           ctcccTCTCAGGCCTCCCCCCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCCCCGAACCGACGCCAACCTtctc                                             <  2:3815132/69‑1 (MQ=255)
                            tcccTCTCGGCCTCCCCCGCCAAACTNGCCGT‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTcc                                            <  1:448714/70‑1 (MQ=255)
                             tCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAAACCTCTCCg                                           >  2:5234974/1‑72 (MQ=255)
                             cccTCTCGGGCTTCGCCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCg                                           <  2:2900553/71‑1 (MQ=255)
                             cccTCGCGGGCTCCACCGCCAAACTGGCCGG‑TACTGGGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCg                                           <  2:6182222/71‑1 (MQ=255)
                             cccTCCCTGGCTCCCCCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCg                                           <  2:3757890/71‑1 (MQ=255)
                              ccTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCgc                                          >  2:5091979/1‑72 (MQ=255)
                              ccTCTCGGCCTCCCCCGCCAAACGGGCCGG‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCgc                                          <  2:1202978/72‑1 (MQ=255)
                                tctctGGCTTCACCGCCAAACTGGCCGG‑CACTGAGGGC‑GGCATCCCCGCACCGACCCCCAACCTCTCCgcct                                        >  2:4482990/1‑70 (MQ=255)
                                 ctctGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCgcttg                                       <  2:6547802/72‑1 (MQ=255)
                                    tGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCgcatgctt                                    >  2:1288512/1‑72 (MQ=255)
                                    tGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCAACGAACCGACGCCAAACCTCTTCgcctgctt                                    >  1:2027588/1‑72 (MQ=255)
                                     ggCTCCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTc                                   <  2:3474322/72‑1 (MQ=255)
                                      ccTTCACCGCCAAACGGGCCGG‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCg                                  <  2:5697712/71‑1 (MQ=255)
                                        cccACCCCAAAACTGCCCGG‑TACGGGNCGT‑GCCACCACCAAACCGACGCCAACCTTCTCCGCTTGCTTCGgc                                <  2:716311/70‑1 (MQ=255)
                                         tCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCCCTTGCTTCcgcg                               >  2:205552/1‑72 (MQ=255)
                                          ccccGCCAACCGGGCCGG‑TACGGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGgcgc                              <  1:375358/70‑1 (MQ=255)
                                            ccGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCgg                            <  1:444281/72‑1 (MQ=255)
                                            ccGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCgg                            <  1:4369960/72‑1 (MQ=255)
                                             ccccAACCTGGCGGG‑TCCTGGCCGG‑GGCCTCCCCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGc                           <  1:2351192/70‑1 (MQ=255)
                                              cccACACGGGCCGG‑TACTGAGCGT‑GGCATCCCCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCa                          <  2:2091938/71‑1 (MQ=255)
                                                 caccTGGCGGG‑TAGTGGCCGG‑GCCATCCCCGACCCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTc                       <  1:954890/69‑1 (MQ=255)
                                                  aaCTGGCCGG‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTcc                      <  2:6477566/72‑1 (MQ=255)
                                                  aaCTGCCCGT‑TACTGAGCTT‑GCAACCACCAAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTcc                      <  2:1159703/72‑1 (MQ=255)
                                                   ccTGGCCGG‑TACTGCGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCt                     <  1:4098763/71‑1 (MQ=255)
                                                   ccTGCCCGG‑TACGGGGCGT‑GGCATCACCGAACCGACGCCACCCTTCTCCGCTTGCTTCGGCGCGGCATTCCt                     <  2:862073/71‑1 (MQ=255)
                                                   aCTGGCCGG‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCt                     <  2:4973688/72‑1 (MQ=255)
                                                    cTGGCCGG‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCTg                    <  2:4544932/72‑1 (MQ=255)
                                                      ggacGG‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCTGTc                  <  2:5062326/69‑1 (MQ=255)
                                                      gcccGG‑TACTGAGCGT‑GGCATCCCCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCTGTc                  <  1:4011709/70‑1 (MQ=255)
                                                       gCCGG‑TACTGAGCGT‑GGCATCCCCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCTGTCg                 <  2:5903253/72‑1 (MQ=255)
                                                        cggg‑TACTGAGCGT‑GGCANCACCGAACCAACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCTGTCGc                <  1:2559085/70‑1 (MQ=255)
                                                         ggg‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCTGTCGCt               <  1:2364936/71‑1 (MQ=255)
                                                         cGG‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCTGTCGCt               <  2:6353063/72‑1 (MQ=255)
                                                         cGG‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCCCCTGCTTCGGCGCCGGCTTCCTGTCGCt               >  1:5383003/1‑72 (MQ=255)
                                                          gg‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCTGTCGCTg              <  2:1498097/72‑1 (MQ=255)
                                                          gg‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGCCGTTCCTGTCGCTg              >  1:6584047/1‑72 (MQ=255)
                                                          gg‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCCTGCTTCCGCGCGGCCTTCCTGTCCCTg              >  1:5562781/1‑72 (MQ=255)
                                                          cgttACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCTGTCGCt               <  1:4807484/69‑1 (MQ=255)
                                                           g‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCCTCTCCGCTTTCTTCGGCGCGGCATTCCTGTCCCTGc             >  2:6675879/1‑72 (MQ=255)
                                                             tACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGGATTTCTTTCGCtcac            >  2:3093502/1‑69 (MQ=255)
                                                             tACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCTGTGGCTGCa            <  2:5029411/72‑1 (MQ=255)
                                                             tACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCTGTCGCTGCa            >  2:4445116/1‑72 (MQ=255)
                                                              aCTGGGCGT‑GGCATCGCCGACCCGACGCCAACTTTCGCCGCTTGCTTCGGCGCGGCATTCCTGTCGCTGCAc           <  2:6596175/72‑1 (MQ=255)
                                                              aCTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGGCTTCCCGTCGCCGCAc           >  1:3174089/1‑72 (MQ=255)
                                                               cTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCTGTCGCTGCAcc          >  1:1905553/1‑72 (MQ=255)
                                                                taaGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCTGTCGCTGCAccc         <  2:4610277/70‑1 (MQ=255)
                                                                 gAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCTGTCGCTGCACCCg        <  2:3663026/72‑1 (MQ=255)
                                                                 gAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCTGTCGCTGCACACg        >  2:4266395/1‑72 (MQ=255)
                                                                 gAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCAGTCCTGTCGCTGCACCCg        >  1:1013978/1‑72 (MQ=255)
                                                                  aGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCTGTCGCTGCACCCGa       >  1:346434/1‑72 (MQ=255)
                                                                   gCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCTGTCGCTGCACCCGAc      >  2:6188179/1‑72 (MQ=255)
                                                                    cGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCTGTCGCTGCACCCGCCt     >  2:1044839/1‑72 (MQ=255)
                                                                    cGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCAGTCCCGGCGCCGCACCCGCCt     >  2:2735313/1‑72 (MQ=255)
                                                                     gT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCTGTCGCTGCACCCGACTc    >  1:2600420/1‑72 (MQ=255)
                                                                     gT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCTGTCGCTGCACCCGACTc    <  1:5811984/72‑1 (MQ=255)
                                                                     gT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCTGTCGCTGCACCCGACTc    >  2:3372499/1‑72 (MQ=255)
                                                                      t‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCTGTCGCTGCACCCGACTCa   >  1:5211802/1‑72 (MQ=255)
                                                                      ggggCATCCCCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCTGTCGCTGCACCCGACTc    <  2:3069113/70‑1 (MQ=255)
                                                                        ggCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCTGTCGCTGCACCCGACTCAg  >  1:4964570/1‑72 (MQ=255)
                                                                         |                                                                      
CCTGACTGCCGATCAAACCCAGTATCACTTCCTCTCTGGCTTCACCGCCAAACTGGCCGG‑TACTGAGCGT‑GGCATCACCGAACCGACGCCAACCTTCTCCGCTTGCTTCGGCGCGGCATTCCTGTCGCTGCACCCGACTCAG  >  NC_012967/3462620‑3462761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.